Restriction map for
pF1KA0162
(Circular) (Six-base) MAPSORT of: pF1KA0162.seq Check: 9529 from: 1 to: 8291
pF1KA0162 8291 bp
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1908 2385 4713 1908
Size: 477 2328 5486
Fragments arranged by size:
5486 2328 477
AccI GT'mk_AC
Cuts at: 1496 5329 1496
Size: 3833 4458
Acc65I G'GTAC_C
Cuts at: 174 4592 5307 174
Size: 4418 715 3158
Fragments arranged by size:
4418 3158 715
AclI AA'CG_TT
Cuts at: 8131 8131
Size: 8291
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1052 1134 1594 2105 2333 3130 3139 4043 4266
Size: 82 460 511 228 797 9 904 223
Cuts at: 4266 6116 6548 6901 7563 1052
Size: 1850 432 353 662 1780
Fragments arranged by size:
1850 1780 904 797 662 511 460 432
353 228 223 82 9
AflIII A'CryG_T
Cuts at: 1673 5643 7443 7724 1673
Size: 3970 1800 281 2240
Fragments arranged by size:
3970 2240 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 2845 2845
Size: 8291
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4525 4525
Size: 8291
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3878 3910 3878
Size: 32 8259
AlwNI CAG_nnn'CTG
Cuts at: 684 1855 1923 2736 5151 5193 5596 7034 684
Size: 1171 68 813 2415 42 403 1438 1941
Fragments arranged by size:
2415 1941 1438 1171 813 403 68 42
ApaI G_GGCC'C
Cuts at: 1166 1166
Size: 8291
ApaLI G'TGCA_C
Cuts at: 7129 7129
Size: 8291
ApoI r'AATT_y
Cuts at: 193 426 781 2620 2884 2989 3835 4318 5295
Size: 233 355 1839 264 105 846 483 977
Cuts at: 5295 7644 7900 193
Size: 2349 256 584
Fragments arranged by size:
2349 1839 977 846 584 483 355 264
256 233 105
AseI AT'TA_AT
Cuts at: 20 20
Size: 8291
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8291
AvaI C'yCGr_G
Cuts at: 179 1735 3139 4376 4541 5311 179
Size: 1556 1404 1237 165 770 3159
Fragments arranged by size:
3159 1556 1404 1237 770 165
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 442 475 442
Size: 33 8258
BamHI G'GATC_C
Cuts at: 1328 4656 5316 1328
Size: 3328 660 4303
Fragments arranged by size:
4303 3328 660
BanI G'GyrC_C
Cuts at: 174 1224 1867 2373 4592 5307 5721 5969 6004
Size: 1050 643 506 2219 715 414 248 35
Cuts at: 6004 174
Size: 2461
Fragments arranged by size:
2461 2219 1050 715 643 506 414 248
35
BanII G_rGCy'C
Cuts at: 929 1166 1434 1652 1805 1907 2099 3372 5305
Size: 237 268 218 153 102 192 1273 1933
Cuts at: 5305 6335 929
Size: 1030 2885
Fragments arranged by size:
2885 1933 1273 1030 268 237 218 192
153 102
BbeI G_GCGC'C
Cuts at: 1871 5725 5973 1871
Size: 3854 248 4189
Fragments arranged by size:
4189 3854 248
BbsI GAAGACnn'nnnn_
Cuts at: 883 2093 2918 3180 883
Size: 1210 825 262 5994
Fragments arranged by size:
5994 1210 825 262
BbvCI CC'TCA_GC
Cuts at: 134 848 1924 134
Size: 714 1076 6501
Fragments arranged by size:
6501 1076 714
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1645 1679 1645
Size: 34 8257
BciVI GTATCCnnnnn_n'
Cuts at: 712 4525 6189 7245 8291 712
Size: 3813 1664 1056 1046 712
Fragments arranged by size:
3813 1664 1056 1046 712
BclI T'GATC_A
Cuts at: 246 5811 246
Size: 5565 2726
BglI GCCn_nnn'nGGC
Cuts at: 595 604 4858 4874 595
Size: 9 4254 16 4012
Fragments arranged by size:
4254 4012 16 9
BglII A'GATC_T
Cuts at: 1388 3551 4604 4746 5806 7843 1388
Size: 2163 1053 142 1060 2037 1836
Fragments arranged by size:
2163 2037 1836 1060 1053 142
BlpI GC'TnA_GC
Cuts at: 3496 5403 3496
Size: 1907 6384
Bme1580I G_kGCm'C
Cuts at: 1166 3703 3772 5916 6009 7133 1166
Size: 2537 69 2144 93 1124 2324
Fragments arranged by size:
2537 2324 2144 1124 93 69
BmrI ACTGGGnnnn_n'
Cuts at: 923 1012 4537 4848 5136 5503 5584 5919 8063
Size: 89 3525 311 288 367 81 335 2144
Cuts at: 8063 923
Size: 1151
Fragments arranged by size:
3525 2144 1151 367 335 311 288 89
81
BmtI G_CTAG'C
Cuts at: 1562 1562
Size: 8291
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6658 6658
Size: 8291
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 596 835 1087 1832 2813 3460 4562 4848
Size: 181 239 252 745 981 647 1102 286
Cuts at: 4848 5005 415
Size: 157 3701
Fragments arranged by size:
3701 1102 981 745 647 286 252 239
181 157
Bpu10I CC'TnA_GC
Cuts at: 134 848 1646 1924 134
Size: 714 798 278 6501
Fragments arranged by size:
6501 798 714 278
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1444 1636 2053 2278 2582 2911 3196 5467 6346
Size: 192 417 225 304 329 285 2271 879
Cuts at: 6346 7060 7358 1444
Size: 714 298 2377
Fragments arranged by size:
2377 2271 879 714 417 329 304 298
285 225 192
BsaI GGTCTCn'nnnn_
Cuts at: 3577 4144 7995 3577
Size: 567 3851 3873
Fragments arranged by size:
3873 3851 567
BsaAI yAC'GTr
Cuts at: 4914 5644 4914
Size: 730 7561
BsaBI GATnn'nnATC
Cuts at: 6 3356 5805 7842 6
Size: 3350 2449 2037 455
Fragments arranged by size:
3350 2449 2037 455
BsaHI Gr'CG_yC
Cuts at: 1868 2675 3639 5722 5970 6672 8168 1868
Size: 807 964 2083 248 702 1496 1991
Fragments arranged by size:
2083 1991 1496 964 807 702 248
BsaWI w'CCGG_w
Cuts at: 3518 4589 5480 6001 6679 7090 7237 7511 3518
Size: 1071 891 521 678 411 147 274 4298
Fragments arranged by size:
4298 1071 891 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 808 838 1252 1282 3211 3241 4560 4590 808
Size: 30 414 30 1929 30 1319 30 4509
Fragments arranged by size:
4509 1929 1319 414 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 151 220 235 238 241 244 247 325 436
Size: 69 15 3 3 3 3 78 111
Cuts at: 436 517 599 631 634 652 1645 2089 2182
Size: 81 82 32 3 18 993 444 93
Cuts at: 2182 2457 2977 3562 4060 4276 4453 151
Size: 275 520 585 498 216 177 3989
Fragments arranged by size:
3989 993 585 520 498 444 275 216
177 111 93 82 81 78 69 32
18 15 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 1165 1319 1696 3152 4725 4848 4931 5054 5060
Size: 154 377 1456 1573 123 83 123 6
Cuts at: 5060 5212 5716 6474 7139 7547 1165
Size: 152 504 758 665 408 1909
Fragments arranged by size:
1909 1573 1456 758 665 504 408 377
154 152 123 123 83 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2233 2296 2919 3956 2233
Size: 63 623 1037 6568
Fragments arranged by size:
6568 1037 623 63
BsiEI CG_ry'CG
Cuts at: 104 5833 5879 7109 104
Size: 5729 46 1230 1286
Fragments arranged by size:
5729 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1652 5305 6083 6273 7133 1652
Size: 3653 778 190 860 2810
Fragments arranged by size:
3653 2810 860 778 190
BsmI GAATG_Cn'
Cuts at: 1080 2441 4770 1080
Size: 1361 2329 4601
Fragments arranged by size:
4601 2329 1361
BsmBI CGTCTCn'nnnn_
Cuts at: 2853 2853
Size: 8291
Bsp1286I G_dGCh'C
Cuts at: 929 1166 1434 1652 1805 1907 2099 3372 3703
Size: 237 268 218 153 102 192 1273 331
Cuts at: 3703 3772 5305 5916 6009 6083 6273 6335 7133
Size: 69 1533 611 93 74 190 62 798
Cuts at: 7133 929
Size: 2087
Fragments arranged by size:
2087 1533 1273 798 611 331 268 237
218 192 190 153 102 93 74 69
62
BspEI T'CCGG_A
Cuts at: 3518 3518
Size: 8291
BspHI T'CATG_A
Cuts at: 3191 3908 6723 3191
Size: 717 2815 4759
Fragments arranged by size:
4759 2815 717
BspMI ACCTGCnnnn'nnnn_
Cuts at: 995 1763 1908 2385 3955 4713 5341 5555 5857
Size: 768 145 477 1570 758 628 214 302
Cuts at: 5857 6238 995
Size: 381 3048
Fragments arranged by size:
3048 1570 768 758 628 477 381 302
214 145
BsrBI CCG'CTC
Cuts at: 50 1010 2583 3533 6583 6637 8121 8286 50
Size: 960 1573 950 3050 54 1484 165 55
Fragments arranged by size:
3050 1573 1484 960 950 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2456 3035 3621 3626 3681 4805 5243 6203 2456
Size: 579 586 5 55 1124 438 960 4544
Fragments arranged by size:
4544 1124 960 586 579 438 55 5
BsrFI r'CCGG_y
Cuts at: 461 2225 3817 6289 6470 461
Size: 1764 1592 2472 181 2282
Fragments arranged by size:
2472 2282 1764 1592 181
BsrGI T'GTAC_A
Cuts at: 1676 2709 1676
Size: 1033 7258
BssSI C'ACGA_G
Cuts at: 1777 6562 7270 1777
Size: 4785 708 2798
Fragments arranged by size:
4785 2798 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3322 4372 5151 5668 3322
Size: 1050 779 517 5945
Fragments arranged by size:
5945 1050 779 517
BstBI TT'CG_AA
Cuts at: 4322 6652 4322
Size: 2330 5961
BstEII G'GTnAC_C
Cuts at: 3159 3159
Size: 8291
BstXI CCAn_nnnn'nTGG
Cuts at: 1449 1696 4856 1449
Size: 247 3160 4884
Fragments arranged by size:
4884 3160 247
BstYI r'GATC_y
Cuts at: 1 1064 1328 1388 3113 3551 4604 4656 4746
Size: 1063 264 60 1725 438 1053 52 90
Cuts at: 4746 5316 5783 5806 6141 6387 6693 6705 6791
Size: 570 467 23 335 246 306 12 86
Cuts at: 6791 7843 1
Size: 1052 449
Fragments arranged by size:
1725 1063 1053 1052 570 467 449 438
335 306 264 246 90 86 60 52
23 12
BtgI C'CryG_G
Cuts at: 590 602 1796 2237 4154 4361 590
Size: 12 1194 441 1917 207 4520
Fragments arranged by size:
4520 1917 1194 441 207 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5267 6241 6422 6585 5267
Size: 974 181 163 6973
Fragments arranged by size:
6973 974 181 163
BtsI GCAGTG_nn'
Cuts at: 330 1306 3323 3703 3897 4893 4908 5152 5534
Size: 976 2017 380 194 996 15 244 382
Cuts at: 5534 330
Size: 3087
Fragments arranged by size:
3087 2017 996 976 382 380 244 194
15
DraI TTT'AAA
Cuts at: 2027 5290 2027
Size: 3263 5028
DraIII CAC_nnn'GTG
Cuts at: 2211 2487 3167 4301 2211
Size: 276 680 1134 6201
Fragments arranged by size:
6201 1134 680 276
DrdI GACnn_nn'nnGTC
Cuts at: 3811 5997 7341 3811
Size: 2186 1344 4761
Fragments arranged by size:
4761 2186 1344
EaeI y'GGCC_r
Cuts at: 354 1521 1712 1814 2240 2282 2774 3862 4943
Size: 1167 191 102 426 42 492 1088 1081
Cuts at: 4943 5876 6050 6441 6468 7813 354
Size: 933 174 391 27 1345 832
Fragments arranged by size:
1345 1167 1088 1081 933 832 492 426
391 191 174 102 42 27
EagI C'GGCC_G
Cuts at: 5876 5876
Size: 8291
EarI CTCTTCn'nnn_
Cuts at: 197 358 815 1583 2573 3428 5073 6314 6524
Size: 161 457 768 990 855 1645 1241 210
Cuts at: 6524 197
Size: 1964
Fragments arranged by size:
1964 1645 1241 990 855 768 457 210
161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 717 5155 5190 6499 7241 7387 8101 717
Size: 4438 35 1309 742 146 714 907
Fragments arranged by size:
4438 1309 907 742 714 146 35
EcoICRI GAG'CTC
Cuts at: 1650 5303 1650
Size: 3653 4638
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 596 835 1052 1087 1134 1594 1832 2105
Size: 181 239 217 35 47 460 238 273
Cuts at: 2105 2333 2813 3130 3139 3460 4043 4266 4562
Size: 228 480 317 9 321 583 223 296
Cuts at: 4562 4848 5005 6116 6548 6901 7563 415
Size: 286 157 1111 432 353 662 1143
Fragments arranged by size:
1143 1111 662 583 480 460 432 353
321 317 296 286 273 239 238 228
223 217 181 157 47 35 9
EcoNI CCTnn'n_nnAGG
Cuts at: 418 3092 4664 418
Size: 2674 1572 4045
Fragments arranged by size:
4045 2674 1572
EcoO109I rG'GnC_Cy
Cuts at: 597 1162 1191 1642 2871 3085 3098 3178 3513
Size: 565 29 451 1229 214 13 80 335
Cuts at: 3513 4380 4694 5430 597
Size: 867 314 736 3458
Fragments arranged by size:
3458 1229 867 736 565 451 335 314
214 80 29 13
EcoRI G'AATT_C
Cuts at: 4318 5295 4318
Size: 977 7314
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4491 6310 4491
Size: 1819 6472
FspI TGC'GCA
Cuts at: 6072 6072
Size: 8291
HaeII r_GCGC'y
Cuts at: 1871 5725 5973 7203 7974 1871
Size: 3854 248 1230 771 2188
Fragments arranged by size:
3854 2188 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 625 657 1456 1488 2831 2863 3211 3243 4329
Size: 32 799 32 1343 32 348 32 1086
Cuts at: 4329 4361 6133 6163 6165 6195 625
Size: 32 1772 30 2 30 2721
Fragments arranged by size:
2721 1772 1343 1086 799 348 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2763 3027 3566 5330 2763
Size: 264 539 1764 5724
Fragments arranged by size:
5724 1764 539 264
HindIII A'AGCT_T
Cuts at: 709 709
Size: 8291
HpaI GTT'AAC
Cuts at: 3566 3566
Size: 8291
KasI G'GCGC_C
Cuts at: 1867 5721 5969 1867
Size: 3854 248 4189
Fragments arranged by size:
4189 3854 248
KpnI G_GTAC'C
Cuts at: 178 4596 5311 178
Size: 4418 715 3158
Fragments arranged by size:
4418 3158 715
MfeI C'AATT_G
Cuts at: 2358 2358
Size: 8291
MluI A'CGCG_T
Cuts at: 7724 7724
Size: 8291
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1135 4203 5129 7050 7234 1135
Size: 3068 926 1921 184 2192
Fragments arranged by size:
3068 2192 1921 926 184
MscI TGG'CCA
Cuts at: 356 1714 1816 2242 2284 2776 4945 6052 356
Size: 1358 102 426 42 492 2169 1107 2595
Fragments arranged by size:
2595 2169 1358 1107 492 426 102 42
MslI CAynn'nnrTG
Cuts at: 1277 1447 4250 5236 6280 6407 1277
Size: 170 2803 986 1044 127 3161
Fragments arranged by size:
3161 2803 1044 986 170 127
MspA1I CmG'CkG
Cuts at: 1323 1773 1798 2736 3156 3761 4071 4415 5190
Size: 450 25 938 420 605 310 344 775
Cuts at: 5190 5402 5716 6076 6860 7105 1323
Size: 212 314 360 784 245 2509
Fragments arranged by size:
2509 938 784 775 605 450 420 360
344 314 310 245 212 25
NaeI GCC'GGC
Cuts at: 6472 6472
Size: 8291
NarI GG'CG_CC
Cuts at: 1868 5722 5970 1868
Size: 3854 248 4189
Fragments arranged by size:
4189 3854 248
NcoI C'CATG_G
Cuts at: 590 602 2237 4154 4361 590
Size: 12 1635 1917 207 4520
Fragments arranged by size:
4520 1917 1635 207 12
NgoMIV G'CCGG_C
Cuts at: 6470 6470
Size: 8291
NheI G'CTAG_C
Cuts at: 1558 1558
Size: 8291
NspI r_CATG'y
Cuts at: 1677 2953 4249 5344 5844 7447 1677
Size: 1276 1296 1095 500 1603 2521
Fragments arranged by size:
2521 1603 1296 1276 1095 500
PasI CC'CwG_GG
Cuts at: 1194 3231 3645 1194
Size: 2037 414 5840
Fragments arranged by size:
5840 2037 414
PciI A'CATG_T
Cuts at: 1673 7443 1673
Size: 5770 2521
PflMI CCAn_nnn'nTGG
Cuts at: 1476 4248 4301 4362 5126 1476
Size: 2772 53 61 764 4641
Fragments arranged by size:
4641 2772 764 61 53
PfoI T'CCnGG_A
Cuts at: 557 714 1103 2816 2867 5267 557
Size: 157 389 1713 51 2400 3581
Fragments arranged by size:
3581 2400 1713 389 157 51
PmeI GTTT'AAAC
Cuts at: 5290 5290
Size: 8291
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2797 2829 6737 6769 2797
Size: 32 3908 32 4319
Fragments arranged by size:
4319 3908 32 32
PpuMI rG'GwC_Cy
Cuts at: 2871 3085 3098 3178 3513 4380 4694 2871
Size: 214 13 80 335 867 314 6468
Fragments arranged by size:
6468 867 335 314 214 80 13
PspOMI G'GGCC_C
Cuts at: 1162 1162
Size: 8291
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2240 2272 2240
Size: 32 8259
PstI C_TGCA'G
Cuts at: 1892 3366 4032 4414 5156 5338 6023 1892
Size: 1474 666 382 742 182 685 4160
Fragments arranged by size:
4160 1474 742 685 666 382 182
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8291
PvuII CAG'CTG
Cuts at: 1323 2736 3156 5716 6076 1323
Size: 1413 420 2560 360 3538
Fragments arranged by size:
3538 2560 1413 420 360
RsrII CG'GwC_CG
Cuts at: 6486 6486
Size: 8291
SacI G_AGCT'C
Cuts at: 1652 5305 1652
Size: 3653 4638
SacII CC_GC'GG
Cuts at: 1799 1799
Size: 8291
SalI G'TCGA_C
Cuts at: 5328 5328
Size: 8291
SanDI GG'GwC_CC
Cuts at: 3098 3178 3098
Size: 80 8211
SapI GCTCTTCn'nnn_
Cuts at: 2573 3428 6314 6524 2573
Size: 855 2886 210 4340
Fragments arranged by size:
4340 2886 855 210
SbfI CC_TGCA'GG
Cuts at: 3366 5338 3366
Size: 1972 6319
ScaI AGT'ACT
Cuts at: 7808 7808
Size: 8291
SexAI A'CCwGG_T
Cuts at: 2207 2207
Size: 8291
SfcI C'TryA_G
Cuts at: 33 1068 1888 2340 3362 4028 4410 5152 5334
Size: 1035 820 452 1022 666 382 742 182
Cuts at: 5334 6019 6987 7178 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 1035 1022 968 820 742 685 666
452 382 191 182
SfiI GGCCn_nnn'nGGCC
Cuts at: 595 595
Size: 8291
SfoI GGC'GCC
Cuts at: 1869 5723 5971 1869
Size: 3854 248 4189
Fragments arranged by size:
4189 3854 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8291
SmaI CCC'GGG
Cuts at: 4378 4543 5313 4378
Size: 165 770 7356
Fragments arranged by size:
7356 770 165
SmlI C'TyrA_G
Cuts at: 1459 1651 2068 2293 2597 2926 3139 3211 5446
Size: 192 417 225 304 329 213 72 2235
Cuts at: 5446 6361 7075 7337 1459
Size: 915 714 262 2413
Fragments arranged by size:
2413 2235 915 714 417 329 304 262
225 213 192 72
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8291
SphI G_CATG'C
Cuts at: 5344 5844 5344
Size: 500 7791
StuI AGG'CCT
Cuts at: 2338 3058 2338
Size: 720 7571
StyI C'CwwG_G
Cuts at: 590 602 1805 1817 2237 2318 2430 4154 4361
Size: 12 1203 12 420 81 112 1724 207
Cuts at: 4361 4824 5425 5779 590
Size: 463 601 354 3102
Fragments arranged by size:
3102 1724 1203 601 463 420 354 207
112 81 12 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 421 1142 4118 5874 6007 6466 6675 421
Size: 721 2976 1756 133 459 209 2037
Fragments arranged by size:
2976 2037 1756 721 459 209 133
TatI w'GTAC_w
Cuts at: 1676 2709 2981 7806 1676
Size: 1033 272 4825 2161
Fragments arranged by size:
4825 2161 1033 272
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1297 1329 4212 4244 4296 4328 7808 7840 1297
Size: 32 2883 32 52 32 3480 32 1748
Fragments arranged by size:
3480 2883 1748 52 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6088 6088
Size: 8291
XbaI T'CTAG_A
Cuts at: 5322 5322
Size: 8291
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 195 3073 3171 4876 5265 5786 195
Size: 2878 98 1705 389 521 2700
Fragments arranged by size:
2878 2700 1705 521 389 98
XhoI C'TCGA_G
Cuts at: 3139 3139
Size: 8291
XmaI C'CCGG_G
Cuts at: 4376 4541 5311 4376
Size: 165 770 7356
Fragments arranged by size:
7356 770 165
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AscI AvrII BfrBI
BmgBI BsiWI BssHII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI NdeI NotI NruI NsiI PacI PmlI
PshAI PsiI PspXI SgrAI SnaBI SrfI SspI SwaI
XmnI ZraI