Restriction map for
pF1KA0015
(Circular) (Six-base) MAPSORT of: pF1KA0015.seq Check: 38 from: 1 to: 4475
pF1KA0015 4475 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 525 525
Size: 4475
AatII G_ACGT'C
Cuts at: 1202 1202
Size: 4475
AccI GT'mk_AC
Cuts at: 1513 1513
Size: 4475
Acc65I G'GTAC_C
Cuts at: 1491 1491
Size: 4475
AclI AA'CG_TT
Cuts at: 4315 4315
Size: 4475
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2300 2732 3085 3747 2300
Size: 432 353 662 3028
Fragments arranged by size:
3028 662 432 353
AflIII A'CryG_T
Cuts at: 626 1115 1827 3627 3908 626
Size: 489 712 1800 281 1193
Fragments arranged by size:
1800 1193 712 489 281
AleI CACnn'nnGTG
Cuts at: 877 877
Size: 4475
AlwNI CAG_nnn'CTG
Cuts at: 573 787 1444 1780 3218 573
Size: 214 657 336 1438 1830
Fragments arranged by size:
1830 1438 657 336 214
ApaLI G'TGCA_C
Cuts at: 745 3313 745
Size: 2568 1907
ApoI r'AATT_y
Cuts at: 388 1479 3828 4084 388
Size: 1091 2349 256 779
Fragments arranged by size:
2349 1091 779 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4475
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
AvaI C'yCGr_G
Cuts at: 551 922 1142 1298 1495 551
Size: 371 220 156 197 3531
Fragments arranged by size:
3531 371 220 197 156
BamHI G'GATC_C
Cuts at: 1500 1500
Size: 4475
BanI G'GyrC_C
Cuts at: 537 868 1491 1905 2153 2188 537
Size: 331 623 414 248 35 2824
Fragments arranged by size:
2824 623 414 331 248 35
BanII G_rGCy'C
Cuts at: 123 798 1159 1268 1312 1489 2519 123
Size: 675 361 109 44 177 1030 2079
Fragments arranged by size:
2079 1030 675 361 177 109 44
BbeI G_GCGC'C
Cuts at: 1909 2157 1909
Size: 248 4227
BbsI GAAGACnn'nnnn_
Cuts at: 1092 1332 1092
Size: 240 4235
BbvCI CC'TCA_GC
Cuts at: 155 155
Size: 4475
BciVI GTATCCnnnnn_n'
Cuts at: 2373 3429 4475 2373
Size: 1056 1046 2373
Fragments arranged by size:
2373 1056 1046
BclI T'GATC_A
Cuts at: 1995 1995
Size: 4475
BglII A'GATC_T
Cuts at: 1990 4027 1990
Size: 2037 2438
BlpI GC'TnA_GC
Cuts at: 253 284 1587 253
Size: 31 1303 3141
Fragments arranged by size:
3141 1303 31
Bme1580I G_kGCm'C
Cuts at: 749 2100 2193 3317 749
Size: 1351 93 1124 1907
Fragments arranged by size:
1907 1351 1124 93
BmgBI CAC'GTC
Cuts at: 912 912
Size: 4475
BmrI ACTGGGnnnn_n'
Cuts at: 1687 1768 2103 4247 1687
Size: 81 335 2144 1915
Fragments arranged by size:
2144 1915 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1326 2842 1326
Size: 1516 2959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 1065 1074 1387 1413 137
Size: 928 9 313 26 3199
Fragments arranged by size:
3199 928 313 26 9
Bpu10I CC'TnA_GC
Cuts at: 155 155
Size: 4475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1651 2530 3244 3542 1651
Size: 879 714 298 2584
Fragments arranged by size:
2584 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 154 582 1441 4179 154
Size: 428 859 2738 450
Fragments arranged by size:
2738 859 450 428
BsaAI yAC'GTr
Cuts at: 627 744 1116 1828 627
Size: 117 372 712 3274
Fragments arranged by size:
3274 712 372 117
BsaBI GATnn'nnATC
Cuts at: 6 1989 4026 6
Size: 1983 2037 455
Fragments arranged by size:
2037 1983 455
BsaHI Gr'CG_yC
Cuts at: 1199 1906 2154 2856 4352 1199
Size: 707 248 702 1496 1322
Fragments arranged by size:
1496 1322 707 702 248
BsaWI w'CCGG_w
Cuts at: 593 1664 2185 2863 3274 3421 3695 593
Size: 1071 521 678 411 147 274 1373
Fragments arranged by size:
1373 1071 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1411 1440 1441 1470 1411
Size: 29 1 29 4416
Fragments arranged by size:
4416 29 29 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 172 277 436 439 454 1294 1425 172
Size: 105 159 3 15 840 131 3222
Fragments arranged by size:
3222 840 159 131 105 15 3
BseYI C'CCAG_C
Cuts at: 198 1900 2658 3323 3731 198
Size: 1702 758 665 408 942
Fragments arranged by size:
1702 942 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 892 892
Size: 4475
BsiEI CG_ry'CG
Cuts at: 104 2017 2063 3293 104
Size: 1913 46 1230 1286
Fragments arranged by size:
1913 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 254 749 1489 2267 2457 3317 254
Size: 495 740 778 190 860 1412
Fragments arranged by size:
1412 860 778 740 495 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1083 1083
Size: 4475
Bsp1286I G_dGCh'C
Cuts at: 123 254 749 798 1159 1268 1312 1489 2100
Size: 131 495 49 361 109 44 177 611
Cuts at: 2100 2193 2267 2457 2519 3317 123
Size: 93 74 190 62 798 1281
Fragments arranged by size:
1281 798 611 495 361 190 177 131
109 93 74 62 49 44
BspEI T'CCGG_A
Cuts at: 593 593
Size: 4475
BspHI T'CATG_A
Cuts at: 2907 2907
Size: 4475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 525 1178 1525 1739 2041 2422 525
Size: 653 347 214 302 381 2578
Fragments arranged by size:
2578 653 381 347 302 214
BsrBI CCG'CTC
Cuts at: 50 855 866 1305 2767 2821 4305 4470 50
Size: 805 11 439 1462 54 1484 165 55
Fragments arranged by size:
1484 1462 805 439 165 55 54 11
BsrDI GCAATG_nn'
Cuts at: 890 2387 890
Size: 1497 2978
BsrFI r'CCGG_y
Cuts at: 517 620 2473 2654 517
Size: 103 1853 181 2338
Fragments arranged by size:
2338 1853 181 103
BssHII G'CGCG_C
Cuts at: 1009 1009
Size: 4475
BssSI C'ACGA_G
Cuts at: 2746 3454 2746
Size: 708 3767
BstAPI GCAn_nnn'nTGC
Cuts at: 1422 1852 1422
Size: 430 4045
BstBI TT'CG_AA
Cuts at: 2836 2836
Size: 4475
BstYI r'GATC_y
Cuts at: 1 1500 1967 1990 2325 2571 2877 2889 2975
Size: 1499 467 23 335 246 306 12 86
Cuts at: 2975 4027 1
Size: 1052 449
Fragments arranged by size:
1499 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 788 1345 788
Size: 557 3918
BtgI C'CryG_G
Cuts at: 229 293 229
Size: 64 4411
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2425 2606 2769 2425
Size: 181 163 4131
Fragments arranged by size:
4131 181 163
BtsI GCAGTG_nn'
Cuts at: 574 1718 574
Size: 1144 3331
DraI TTT'AAA
Cuts at: 1474 1474
Size: 4475
DrdI GACnn_nn'nnGTC
Cuts at: 2181 3525 2181
Size: 1344 3131
EaeI y'GGCC_r
Cuts at: 143 290 519 1073 2060 2234 2625 2652 3997
Size: 147 229 554 987 174 391 27 1345
Cuts at: 3997 143
Size: 621
Fragments arranged by size:
1345 987 621 554 391 229 174 147
27
EagI C'GGCC_G
Cuts at: 2060 2060
Size: 4475
EarI CTCTTCn'nnn_
Cuts at: 124 413 658 2498 2708 124
Size: 289 245 1840 210 1891
Fragments arranged by size:
1891 1840 289 245 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2683 3425 3571 4285 2683
Size: 742 146 714 2873
Fragments arranged by size:
2873 742 714 146
EcoICRI GAG'CTC
Cuts at: 1487 1487
Size: 4475
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 1065 1074 1387 1413 2300 2732 3085 3747
Size: 928 9 313 26 887 432 353 662
Cuts at: 3747 137
Size: 865
Fragments arranged by size:
928 887 865 662 432 353 313 26
9
EcoO109I rG'GnC_Cy
Cuts at: 219 237 318 450 1067 1350 1392 1614 219
Size: 18 81 132 617 283 42 222 3080
Fragments arranged by size:
3080 617 283 222 132 81 42 18
EcoRI G'AATT_C
Cuts at: 388 1479 388
Size: 1091 3384
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2494 2494
Size: 4475
FspI TGC'GCA
Cuts at: 2256 2256
Size: 4475
HaeII r_GCGC'y
Cuts at: 1150 1909 2157 3387 4158 1150
Size: 759 248 1230 771 1467
Fragments arranged by size:
1467 1230 771 759 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1411 1440 1443 1472 2317 2347 2349 2379 1411
Size: 29 3 29 845 30 2 30 3507
Fragments arranged by size:
3507 845 30 30 29 29 3 2
HincII GTy'rAC
Cuts at: 1062 1514 1062
Size: 452 4023
KasI G'GCGC_C
Cuts at: 1905 2153 1905
Size: 248 4227
KpnI G_GTAC'C
Cuts at: 1495 1495
Size: 4475
MluI A'CGCG_T
Cuts at: 3908 3908
Size: 4475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3234 3418 3234
Size: 184 4291
MscI TGG'CCA
Cuts at: 145 292 2236 145
Size: 147 1944 2384
Fragments arranged by size:
2384 1944 147
MslI CAynn'nnrTG
Cuts at: 877 1330 2464 2591 877
Size: 453 1134 127 2761
Fragments arranged by size:
2761 1134 453 127
MspA1I CmG'CkG
Cuts at: 366 564 736 1262 1586 1900 2260 3044 3289
Size: 198 172 526 324 314 360 784 245
Cuts at: 3289 366
Size: 1552
Fragments arranged by size:
1552 784 526 360 324 314 245 198
172
NaeI GCC'GGC
Cuts at: 519 2656 519
Size: 2137 2338
NarI GG'CG_CC
Cuts at: 1906 2154 1906
Size: 248 4227
NcoI C'CATG_G
Cuts at: 229 293 229
Size: 64 4411
NgoMIV G'CCGG_C
Cuts at: 517 2654 517
Size: 2137 2338
NspI r_CATG'y
Cuts at: 1528 2028 3631 1528
Size: 500 1603 2372
Fragments arranged by size:
2372 1603 500
PasI CC'CwG_GG
Cuts at: 241 404 241
Size: 163 4312
PciI A'CATG_T
Cuts at: 3627 3627
Size: 4475
PflMI CCAn_nnn'nTGG
Cuts at: 480 480
Size: 4475
PfoI T'CCnGG_A
Cuts at: 173 173
Size: 4475
PmeI GTTT'AAAC
Cuts at: 1474 1474
Size: 4475
PmlI CAC'GTG
Cuts at: 627 744 627
Size: 117 4358
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2921 2953 2921
Size: 32 4443
PpuMI rG'GwC_Cy
Cuts at: 219 1067 1350 1392 219
Size: 848 283 42 3302
Fragments arranged by size:
3302 848 283 42
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 667 699 667
Size: 32 4443
PstI C_TGCA'G
Cuts at: 863 1522 2207 863
Size: 659 685 3131
Fragments arranged by size:
3131 685 659
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4475
PvuII CAG'CTG
Cuts at: 366 1900 2260 366
Size: 1534 360 2581
Fragments arranged by size:
2581 1534 360
RsrII CG'GwC_CG
Cuts at: 2670 2670
Size: 4475
SacI G_AGCT'C
Cuts at: 1489 1489
Size: 4475
SalI G'TCGA_C
Cuts at: 1512 1512
Size: 4475
SanDI GG'GwC_CC
Cuts at: 1067 1350 1392 1067
Size: 283 42 4150
Fragments arranged by size:
4150 283 42
SapI GCTCTTCn'nnn_
Cuts at: 124 658 2498 2708 124
Size: 534 1840 210 1891
Fragments arranged by size:
1891 1840 534 210
SbfI CC_TGCA'GG
Cuts at: 1522 1522
Size: 4475
ScaI AGT'ACT
Cuts at: 3992 3992
Size: 4475
SfcI C'TryA_G
Cuts at: 33 370 859 1518 2203 3171 3362 33
Size: 337 489 659 685 968 191 1146
Fragments arranged by size:
1146 968 685 659 489 337 191
SfoI GGC'GCC
Cuts at: 1907 2155 1907
Size: 248 4227
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
SmaI CCC'GGG
Cuts at: 553 1144 1300 1497 553
Size: 591 156 197 3531
Fragments arranged by size:
3531 591 197 156
SmlI C'TyrA_G
Cuts at: 1630 2545 3259 3521 1630
Size: 915 714 262 2584
Fragments arranged by size:
2584 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4475
SphI G_CATG'C
Cuts at: 1528 2028 1528
Size: 500 3975
SrfI GCCC'GGGC
Cuts at: 553 553
Size: 4475
StyI C'CwwG_G
Cuts at: 229 293 1609 1963 229
Size: 64 1316 354 2741
Fragments arranged by size:
2741 1316 354 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1015 2058 2191 2650 2859 1015
Size: 1043 133 459 209 2631
Fragments arranged by size:
2631 1043 459 209 133
TatI w'GTAC_w
Cuts at: 3990 3990
Size: 4475
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 947 979 3992 4024 947
Size: 32 3013 32 1398
Fragments arranged by size:
3013 1398 32 32
Tth111I GACn'n_nGTC
Cuts at: 2272 2272
Size: 4475
XbaI T'CTAG_A
Cuts at: 1506 1506
Size: 4475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 300 1219 1970 300
Size: 919 751 2805
Fragments arranged by size:
2805 919 751
XmaI C'CCGG_G
Cuts at: 551 1142 1298 1495 551
Size: 591 156 197 3531
Fragments arranged by size:
3531 591 197 156
ZraI GAC'GTC
Cuts at: 1200 1200
Size: 4475
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmtI BsiWI BsmI
BsrGI BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PshAI PsiI PspOMI PspXI
SacII SexAI SfiI SgrAI SnaBI SspI StuI SwaI
XhoI XmnI