GENSCAN 1.0	Date run:  8-Nov-116	Time: 04:05:21

Sequence gi568815583f:74519197_74729880 : 210684 bp : 46.98% C+G : Isochore 2 (43 - 51 C+G%)

Parameter matrix: HumanIso.smat

Predicted genes/exons:

Gn.Ex Type S .Begin ...End .Len Fr Ph I/Ac Do/T CodRg P.... Tscr..
----- ---- - ------ ------ ---- -- -- ---- ---- ----- ----- ------

 1.01 Intr +  18564  18627   64  2  1   43   91    84 0.318   2.49
 1.02 Intr +  21920  22134  215  0  2   94  111    64 0.495   7.73
 1.03 Term +  22876  22959   84  0  0   42   38    95 0.564  -2.45
 1.04 PlyA +  23343  23348    6                              -0.45

 2.00 Prom +  23833  23872   40                              -3.66
 2.01 Init +  24741  25292  552  2  0   95   82   356 0.839  30.65
 2.02 Intr +  53666  53737   72  1  0  135   66    31 0.567   5.20
 2.03 Intr +  53936  54008   73  1  1    4   84   143 0.909   4.48
 2.04 Intr +  70624  70807  184  2  1   47   73   342 0.155  27.45
 2.05 Intr +  71955  72238  284  0  2  121   82   254 0.997  25.16
 2.06 Intr +  72364  72618  255  2  0   28   72   426 0.973  32.42
 2.07 Intr +  73939  74040  102  2  0   85   94   100 0.771  10.45
 2.08 Term +  76415  76578  164  0  2   70   45   150 0.437   7.00
 2.09 PlyA +  78911  78916    6                               1.05

 3.10 PlyA -  79746  79741    6                               1.05
 3.09 Term -  80260  80163   98  2  2   79   46    57 0.352  -1.27
 3.08 Intr -  83975  83794  182  1  2   15  105   113 0.529   5.21
 3.07 Intr -  84584  84491   94  0  1   37   80    63 0.310  -0.58
 3.06 Intr -  85140  85089   52  0  1  114   98     7 0.608   2.78
 3.05 Intr -  89292  89018  275  1  2   54   80   112 0.731   4.26
 3.04 Intr -  91556  91466   91  2  1   89   65    92 0.951   6.57
 3.03 Intr -  92398  92215  184  0  1   80   59   240 0.962  20.09
 3.02 Intr -  92601  92484  118  1  1   97   99     9 0.759   2.42
 3.01 Init -  93689  93647   43  2  1   84   80    -9 0.419  -1.49
 3.00 Prom -  98237  98198   40                              -8.36

 4.00 Prom +  99858  99897   40                             -11.53
 4.01 Init + 100001 100152  152  1  2   55  100   163 0.761  13.71
 4.02 Intr + 100609 101029  421  1  1  112  109   332 0.507  31.65
 4.03 Intr + 101085 101109   25  2  1   61  100   -15 0.319  -5.40
 4.04 Intr + 103298 103364   67  0  1  117   99    78 0.801   9.76
 4.05 Intr + 105706 105822  117  1  0   54   62   226 0.998  16.18
 4.06 Intr + 106606 106772  167  1  2   95   95   168 0.999  17.80
 4.07 Intr + 108156 108262  107  1  2   86   74   127 0.716  11.03
 4.08 Intr + 108343 108472  130  0  1  103   97   208 0.995  23.47
 4.09 Intr + 108774 108856   83  1  2   90   92    90 0.969   8.96
 4.10 Intr + 109408 109487   80  0  2   94  110    79 0.999   9.05
 4.11 Intr + 109746 109836   91  0  1  110   86    77 0.999   9.70
 4.12 Term + 110511 110687  177  2  0   42   48   275 0.969  16.59
 4.13 PlyA + 110897 110902    6                               1.05

 5.07 PlyA - 111384 111379    6                               1.05
 5.06 Term - 113750 113416  335  0  2   82   44   447 0.947  34.47
 5.05 Intr - 116430 116213  218  2  2  105  105   108 0.836  12.35
 5.04 Intr - 121423 121270  154  2  1   75   99    82 0.166   7.13
 5.03 Intr - 136872 136537  336  1  0  110  100   476 0.923  46.49
 5.02 Intr - 152578 152259  320  0  2   85   95   265 0.972  22.50
 5.01 Init - 157824 157745   80  0  2   63   89    73 0.716   5.43
 5.00 Prom - 196442 196403   40                              -3.06

 6.07 PlyA - 196948 196943    6                               1.05
 6.06 Term - 201578 201293  286  1  1  116   43   341 0.928  27.28
 6.05 Intr - 201857 201771   87  1  0  138  100    81 0.999  13.39
 6.04 Intr - 202126 202003  124  2  1  104  110   108 0.999  14.24
 6.03 Intr - 202307 202218   90  0  0  111   82    64 0.996   8.07
 6.02 Intr - 202521 202395  127  0  1   80   60   309 0.999  27.55
 6.01 Intr - 203930 203056  875  0  2   76   94   974 0.071  87.86

Suboptimal exons with probability > 0.800

Exnum Type S .Begin ...End .Len Fr Ph B/Ac Do/T CodRg P.... Tscr..
----- ---- - ------ ------ ---- -- -- ---- ---- ----- ----- ------

S.001 Intr +  53377  53501  125  2  2   98   67    20 0.801   1.20


Predicted peptide sequence(s):

Predicted coding sequence(s):


>gi568815583f:74519197_74729880|GENSCAN_predicted_peptide_1|120_aa
NSPGLSVVTGKIVAIIEKALHAASGFGPAPPPVAPPRPRPRPWLAVQPRGAASGAGPAPA
VAPGCGGVRDAAAAPGPELPPPPPPENPECPAQHSEKMVPQDKKQLFSDWQDLVEKLFRS

>gi568815583f:74519197_74729880|GENSCAN_predicted_CDS_1|363_bp
aactctccagggctttcagtggtgaccggcaaaattgttgccataattgagaaggccctg
catgcagcttccggcttcggtcccgccccgccccctgtggccccgccccgcccccgcccc
cgcccctggctggcggtccagccccgcggcgccgcttccggtgcgggccccgccccggct
gtggcccccggctgcggaggagtccgagacgcagctgccgcgccggggcctgagctgccg
cctcctccgccgcccgaaaacccggagtgccccgcacagcattcagagaagatggtgcca
caagacaagaagcagttgttcagtgactggcaggacttggttgagaagttattccgatcg
tga

>gi568815583f:74519197_74729880|GENSCAN_predicted_peptide_2|561_aa
MEPLQQQQQQQQQQQKQPHLAPLQMDAREKQGQQMREAQFLYAQKLVTQPTLLSATAGRP
SGSTPLGPLARVPPTAAVAQVFERGNMNSEPEEEDGGLEDEDGDDEVAEVAEKETQAASK
YFHVQKVARQDPRVAPMSNLLPAPGLPPHGQQAKEDHTKDASKASPSVSTAGQPNWNLDE
QLKQNGGLAWSDDADGGRGREISRDFAKLYELDGDPERKEFLDDLFVFMQKRGTPINRIP
IMAKQILDLYMLYKLVTEKGGLVEIINKKIWREITKGLNLPTSITSAAFTLRTQYMKYLY
AYECEKKALSSPAELQAAIDGNRREGRRPSYSSSLFGYSPAAATAAAAAGAPALLSPPKI
RFPILGLGSSSGTNTSSPRISPATTLRKGDGAPVTTVPVPNRLAVPVTLASQQAGTRTAA
LEQLRERLESGEPAEKKASRLSEEEQRLVQQAFQRNFFSMARQLPMKIRINGRAEDRAEA
SAAALNLTTSSIGSINMSVDIDGTTYAGVLFAQKPVVHLITGSAPQSLGSSASSSSSSHC
SPSPTSSRGTPSAEPSTSWSL

>gi568815583f:74519197_74729880|GENSCAN_predicted_CDS_2|1686_bp
atggagccacttcagcagcagcagcagcagcagcagcaacaacagaagcagccacacctg
gctcctctgcagatggatgccagagagaagcagggccagcagatgagagaagcccagttc
ttgtatgcccaaaagctggtcacacagccgactctcctttccgccacagctgggagacct
tctggcagcactcccttaggtcccttagccagagttccacccaccgcagcagtggcccaa
gtgtttgaacggggcaacatgaactcagagcctgaggaagaggacggaggtttggaagat
gaggatggggatgatgaagttgcagaggtggctgagaaagaaacccaggctgcttcaaaa
tattttcatgtgcagaaagtagctcgccaagatcccagagtggcacccatgtccaatcta
cttccagcaccagggctcccaccacatggacaacaagctaaagaagaccataccaaagat
gcttccaaggcctcaccttctgtctccacagcaggacagccgaactggaatctggatgag
cagctcaagcagaatggtggtttggcctggagtgatgatgcagatggaggccggggaaga
gagatctctcgagattttgccaagctgtatgaactggacggtgatcctgaaaggaaagag
ttcctggatgacctcttcgtctttatgcagaagagggggacccccatcaaccgaatcccc
atcatggccaaacagatcctggacctgtacatgctgtataagctggtgaccgagaaggga
ggcctggtggagatcatcaacaagaagatctggagggagatcaccaaaggcctaaacctg
cccacatccatcaccagcgccgccttcaccctcaggacgcagtacatgaagtatctgtat
gcctatgagtgtgagaagaaagccttgagttccccagccgagctccaggcagcaattgat
ggcaaccgcagggagggccggcggcccagctacagctcctccctctttggctactcacct
gctgcggctactgctgctgccgctgccggggcccctgcccttctctccccacccaagatc
cgctttcccatccttgggcttggctccagcagtggtaccaataccagtagccctcggata
tccccagcaaccactctcaggaaaggtgatggagccccagtgacaacagtgcctgtgcca
aatcgtctggctgtgcccgtgaccttggcaagccagcaggctggtactcggaccgccgca
ctggagcagctgcgggagcggctggagtcaggggagcctgctgagaagaaggcatcgagg
ctgtctgaggaggagcagcgcctggtgcagcaggccttccagcgcaactttttcagcatg
gcacggcagctccccatgaagatcaggatcaacggcagggcagaagacagagcagaggcc
tcggctgcagcactgaacctgaccacgagtagcattgggagcattaacatgtctgtggac
atcgatggcaccacctatgcaggtgtgctgtttgcccagaagcctgtggtccacctcatc
acggggtctgctccccagagcctcggcagcagcgccagcagcagcagcagctctcactgt
tcaccaagtcctacctcatcccggggcacccccagcgcagagccctccaccagctggtcc
ctctga

>gi568815583f:74519197_74729880|GENSCAN_predicted_peptide_3|378_aa
MESLSLLTGKTEHPGPTEAEPMDLMPSPARCLWNSYFLGALDRVCPPSPTEGCRAPERDW
RRAEKDLWEQLAESRQQRAELASRLRAVPEALSEQALVLQRREHKLGLGWSLKQKHATAV
LLASTLKLSAAGEQLERRCISGPAGVHKTIITEREWGGGDLGSMLYLDCTISSSTLQVLE
VPVPLRKELEAQESHPRLHEEMAEYWKARWHRLAVALKFKEEELQRLQRQSGTWPPQGRP
QQRPQASPRSLQMKAAFLPPIPQELWEPRSAKELESAVWTPPEANGDPGKDVEMEGDVRP
ALRGSWARQYYPYGERCGTQGKRGSEGSGVQGKCSGLQSPAQLGGEVKQFLKGGVLGTVE
GQDISGNSYPAAFVLSSS

>gi568815583f:74519197_74729880|GENSCAN_predicted_CDS_3|1137_bp
atggaaagcctgtcacttctaactggcaagacagagcacccaggtcccactgaggcagag
cccatggacttgatgcccagccctgcccgctgtctctggaactcctatttcctcggggca
ttggaccgtgtctgcccaccaagccccactgaagggtgcagggcccccgagcgggactgg
aggcgggcagagaaggacctgtgggagcagctggcagaaagcaggcagcagagggccgag
ctggcaagccgtcttcgggcagtgccggaggccctgtcggagcaagccctggtgctgcag
aggcgggagcacaagctgggacttggctggtccctaaagcagaaacatgccacagcagtc
ctgctggcctccaccctgaagctgtcagcagctggagagcagctggagaggcggtgtatc
tcgggcccagctggagttcataagacaataatcactgaaagggagtggggtgggggtgat
ctggggtccatgctgtacctggactgcaccatttcttccagtacactccaggtcctggag
gtaccagtgcccctcaggaaagagctagaggcacaagaatcacatcccaggcttcatgag
gagatggctgaatattggaaggcacgctggcaccggctggcagttgccctgaaatttaaa
gaggaggaactgcagagactccagaggcagagtgggacctggcctccccagggcagaccg
cagcagagaccccaggccagcccccggagtctgcaaatgaaagcagcgtttctgcctccc
atcccccaggagctgtgggagcccaggtctgccaaggagctggagtctgcagtgtggact
ccaccagaggccaatggggaccctggaaaggatgtggagatggagggcgatgtgaggcct
gccctcaggggctcctgggctaggcagtactacccgtatggggagcgctgcgggactcag
ggtaaaagagggagtgaaggatcaggggttcagggcaagtgttctggacttcagtcacct
gcacagcttggaggggaagtgaagcagtttcttaaaggaggggtccttggtaccgtggag
ggacaggacatctctgggaattcgtacccagctgcgttcgtgctgagttcttcatga

>gi568815583f:74519197_74729880|GENSCAN_predicted_peptide_4|538_aa
MHHCKRYRSPEPDPYLSYRWKRRRSYSREHEGRLRYPSRREPPPRRSRSRSWRMLSLDRA
LEGGLSTFAPSPLCPLPTSVDAFSTGCPPLALYSGLTTGLLLANSKDPADQRPHPPFGSH
DRLPYQRRYRERRDSDTYRCEERSPSFGEDYYGPSRSRHRRRSRERGPYRTRKHAHHCHK
RRTRSCSSASSPSLRLAGPDEIVGNLGEGTFGKVVECLDHARGKSQVALKIIRNVGKYRE
AARLEINVLKKIKEKDKENKFLCVLMSDWFNFHGHMCIAFELLGKNTFEFLKENNFQPYP
LPHVRHMAYQLCHALRFLHENQLTHTDLKPENILFVNSEFETLYNEHKVLVGSCEEKSVK
NTSIRVADFGSATFDHEHHTTIVATRHYRPPEVILELGWAQPCDVWSIGCILFEYYRGFT
LFQTHENREHLVMMEKILGPIPSHMIHRTRKQKYFYKGGLVWDENSSDGRYVKENCKPLK
SYMLQDSLEHVQLFDLMRRMLEFDPAQRITLAEALLHPFFAGLTPEERSFHTSRNPSR

>gi568815583f:74519197_74729880|GENSCAN_predicted_CDS_4|1617_bp
atgcatcactgtaagcgataccgctcccctgaaccagacccgtacctgagctaccgatgg
aagaggaggaggtcctacagtcgggaacatgaagggagactgcgatacccgtcccgaagg
gagcctcccccacgaagatctcggtccagaagctggaggatgctgtcgttggacagggct
ctggagggcgggcttagtacctttgcaccctcccctctctgcccacttcccaccagtgtg
gatgcttttagcacaggctgtccccctttagccctttacagtggccttaccacaggtctc
ttgcttgctaacagcaaggacccagctgaccagcgtccccatcccccttttggcagccat
gaccgcctgccctaccagaggaggtaccgggagcgccgtgacagcgatacataccggtgt
gaagagcggagcccatcctttggagaggactactatggaccttcacgttctcgtcatcgt
cggcgatcgcgggagagggggccataccggacccgcaagcatgcccaccactgccacaaa
cgccgcaccaggtcttgtagcagcgcctcctcgccttctctcaggctggctggtccggat
gagattgtggggaacctgggtgaaggcacctttggcaaggtggtggagtgcttggaccat
gccagagggaagtctcaggttgccctgaagatcatccgcaacgtgggcaagtaccgggag
gctgcccggctagaaatcaacgtgctcaaaaaaatcaaggagaaggacaaagaaaacaag
ttcctgtgtgtcttgatgtctgactggttcaacttccacggtcacatgtgcatcgccttt
gagctcctgggcaagaacacctttgagttcctgaaggagaataacttccagccttacccc
ctaccacatgtccggcacatggcctaccagctctgccacgcccttagatttctgcatgag
aatcagctgacccatacagacttgaaaccagagaacatcctgtttgtgaattctgagttt
gaaaccctctacaatgagcacaaggtattggtggggagctgtgaggagaagtcagtgaag
aacaccagcatccgagtggctgactttggcagtgccacatttgaccatgagcaccacacc
accattgtggccacccgtcactatcgcccgcctgaggtgatccttgagctgggctgggca
cagccctgtgacgtctggagcattggctgcattctctttgagtactaccggggcttcaca
ctcttccagacccacgaaaaccgagagcacctggtgatgatggagaagatcctagggccc
atcccatcacacatgatccaccgtaccaggaagcagaaatatttctacaaagggggccta
gtttgggatgagaacagctctgacggccggtatgtgaaggagaactgcaaacctctgaag
agttacatgctccaagactccctggagcacgtgcagctgtttgacctgatgaggaggatg
ttagaatttgaccctgcccagcgcatcacactggccgaggccctgctgcaccccttcttt
gctggcctgacccctgaggagcggtccttccacaccagccgcaacccaagcagatga

>gi568815583f:74519197_74729880|GENSCAN_predicted_peptide_5|480_aa
MNDGFCCSTSSSAFGVVSVLDFGHSDRAGDITELKILEIPGPGDNQHFGDLHQTELGPSG
AGCQVGINQNGTGKFVKKPASSSSAPQNIPKRTDVKSQDVAVSPQQQQCSKSYVDRHMES
LSQSKSFRRRHNSWSSSSRHPNQATPKKSGLKNGQMKNKDDECFGDDIEEIPDTDFDFEG
NLALFDKAAVFEEIDTYERRSGTRSRGIPNERPTRYRHDENILESEPIVYRRIIVPHNVS
KEFCTDSGLVVPSISYELHKKLLSVAEKHGLTLERRLEMTGVCASQMALTLLGGPNRLNP
KNVHQRPTVALLCGPHVKGAQGISCGRHLANHDVQVILFLPNFVKMLESITNELSLFSKT
QGQQVSSLKDLPTSPVDLVINCLDCPENVFLRDQPWYKAAVAWANQNRAPVLSIDPPVHE
VEQGIDAKWSLALGLPLPLGEHAGRIYLCDIGIPQQVFQEVGINYHSPFGCKFVIPLHSA

>gi568815583f:74519197_74729880|GENSCAN_predicted_CDS_5|1443_bp
atgaatgacggtttttgttgctccacatcctcgtcagcatttggtgttgtcagtgttttg
gatttcggccattctgacagggcaggtgacattacggagttaaaaattctggagatacca
ggacctggagacaaccaacattttggagaccttcatcaaacagaattaggcccctctggt
gctggctgccaagtgggcatcaatcagaatggcacaggcaagtttgtcaagaagccagcc
tcttccagcagtgcccctcagaatatccctaagaggacagatgtgaagagccaggatgtt
gccgtttccccgcagcagcaacagtgctcaaagagctatgtcgacaggcacatggaatcc
ttgagtcagtccaaaagtttccgtcgtcggcacaactcctggtcatctagtagcaggcac
ccaaatcaggcaactcccaagaaaagtggtttaaagaatggccagatgaagaataaagat
gacgagtgcttcggggatgatattgaggagatcccagacacagattttgattttgaaggg
aacctggctctttttgacaaggcagctgtgtttgaggagattgatacctatgaaaggaga
agtggtacccgttcccggggcatcccaaatgaaaggcccactcggtaccgccatgatgag
aacatcttggagtccgagcccattgtctatcgacggatcatagtgccccacaacgtgagc
aaggagttctgcacggactctggcctggttgtcccaagtatttcctatgagctgcataaa
aagctgttgtccgtggctgagaagcatgggctgacccttgagcggagactggagatgaca
ggtgtgtgtgccagtcagatggcactgaccctcctcggaggacctaacaggttgaatccc
aaaaatgttcaccagaggcctacagtggctctactgtgtggacctcatgtgaagggggct
cagggtatcagctgtggaaggcacctagccaaccatgatgtccaggtcatccttttcctg
cccaattttgtcaagatgttggaatctatcaccaatgagctgtcgctcttcagcaagacc
caaggccaacaagtgtctagcctcaaagatctgcccactagccctgtggacctggtcatc
aactgcctggattgccctgagaacgtcttcctgcgcgatcaaccctggtacaaggcagct
gtggcctgggccaaccagaaccgggcaccagtactcagcatagaccctcctgtgcatgaa
gtcgaacagggcattgatgccaaatggtcactggcactgggcctgcctctgccactgggg
gagcacgcaggccgtatctatttgtgcgacattggcattccccagcaggtcttccaggag
gtgggcatcaactaccactcgccctttggctgcaagtttgttatcccactgcactctgct
tag

>gi568815583f:74519197_74729880|GENSCAN_predicted_peptide_6|529_aa
XATSKIPTLIMLFPISMSATEFLLASVIFCLVFWVIRASRPQVPKGLKNPPGPWGWPLIG
HMLTLGKNPHLALSRMSQQYGDVLQIRIGSTPVVVLSGLDTIRQALVRQGDDFKGRPDLY
TFTLISNGQSMSFSPDSGPVWAARRRLAQNGLKSFSIASDPASSTSCYLEEHVSKEAEVL
ISTLQELMAGPGHFNPYRYVVVSVTNVICAICFGRRYDHNHQELLSLVNLNNNFGEVVGS
GNPADFIPILRYLPNPSLNAFKDLNEKFYSFMQKMVKEHYKTFEKVQSGERQGHIRDITD
SLIEHCQEKQLDENANVQLSDEKIINIVLDLFGAGFDTVTTAISWSLMYLVMNPRVQRKI
QEELDTVIGRSRRPRLSDRSHLPYMEAFILETFRHSSFVPFTIPHSTTRDTSLKGFYIPK
GRCVFVNQWQINHDQKLWVNPSEFLPERFLTPDGAIDKVLSEKVIIFGMGKRKCIGETIA
RWEVFLFLAILLQRVEFSVPLGVKVDMTPIYGLTMKHACCEHFQMQLRS

>gi568815583f:74519197_74729880|GENSCAN_predicted_CDS_6|1590_bp
ncagccacctccaagatccctacactgatcatgcttttcccaatctccatgtcggccacg
gagtttcttctggcctctgtcatcttctgtctggtattctgggtaatcagggcctcaaga
cctcaggtccccaaaggcctgaagaatccaccagggccatggggctggcctctgattggg
cacatgctgaccctgggaaagaacccgcacctggcactgtcaaggatgagccagcagtat
ggggacgtgctgcagatccgaattggctccacacccgtggtggtgctgagcggcctggac
accatccggcaggccctggtgcggcagggcgatgatttcaagggccggcccgacctctac
accttcaccctcatcagtaatggtcagagcatgtccttcagcccagactctggaccagtg
tgggctgcccgccggcgcctggcccagaatggcctgaaaagtttctccattgcctctgac
ccagcctcctcaacctcctgctacctggaagagcatgtgagcaaggaggctgaggtcctg
ataagcacgttgcaggagctgatggcagggcctgggcactttaacccctacaggtatgtg
gtggtatcagtgaccaatgtcatctgtgccatttgctttggccggcgctatgaccacaac
caccaagaactgcttagcctagtcaacctgaataataatttcggggaggtggttggctct
ggaaacccagctgacttcatccctattcttcgctacctacccaacccttccctgaatgcc
ttcaaggacctgaatgagaagttctacagcttcatgcagaagatggtcaaggagcactac
aaaacctttgagaaggtacagtctggggaaaggcagggccacatccgggacatcacagac
agcctgattgagcactgtcaggagaagcagctggatgagaacgccaatgtccagctgtca
gatgagaagatcattaacatcgtcttggacctctttggagctgggtttgacacagtcaca
actgctatctcctggagcctcatgtatttggtgatgaaccccagggtacagagaaagatc
caagaggagctagacacagtgattggcaggtcacggcggccccggctctctgacagatcc
catctgccctatatggaggccttcatcctggagaccttccgacactcttccttcgtcccc
ttcaccatcccccacagcacaacaagagacacaagtttgaaaggcttttacatccccaag
gggcgttgtgtctttgtaaaccagtggcagatcaaccatgaccagaagctatgggtcaac
ccatctgagttcctacctgaacggtttctcacccctgatggtgctatcgacaaggtgtta
agtgagaaggtgattatctttggcatgggcaagcggaagtgtatcggtgagaccattgcc
cgctgggaggtctttctcttcctggctatcctgctgcaacgggtggaattcagcgtgcca
ctgggcgtgaaggtggacatgacccccatctatgggctaaccatgaagcatgcctgctgt
gagcacttccaaatgcagctgcgctcttag