Restriction map for
pF1KE9573
(Linear) (Six-base) MAPSORT of: KIEE9573.seq Check: 678 from: 1 to: 1422
KIEE9573.orf
With 182 enzymes: SgfI *
September 11, 2018 18:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 1036 1422
Size: 445 591 386
Fragments arranged by size:
591 445 386
AflIII A'CryG_T
Cuts at: 0 557 1007 1422
Size: 557 450 415
Fragments arranged by size:
557 450 415
AleI CACnn'nnGTG
Cuts at: 0 487 1422
Size: 487 935
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 679 1422
Size: 679 743
AlwNI CAG_nnn'CTG
Cuts at: 0 1291 1338 1422
Size: 1291 47 84
Fragments arranged by size:
1291 84 47
ApaI G_GGCC'C
Cuts at: 0 1221 1422
Size: 1221 201
ApoI r'AATT_y
Cuts at: 0 579 1229 1422
Size: 579 650 193
Fragments arranged by size:
650 579 193
BanI G'GyrC_C
Cuts at: 0 813 1256 1422
Size: 813 443 166
Fragments arranged by size:
813 443 166
BanII G_rGCy'C
Cuts at: 0 107 632 1221 1422
Size: 107 525 589 201
Fragments arranged by size:
589 525 201 107
BbeI G_GCGC'C
Cuts at: 0 1260 1422
Size: 1260 162
BbvCI CC'TCA_GC
Cuts at: 0 1339 1422
Size: 1339 83
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1357 1391 1422
Size: 1357 34 31
Fragments arranged by size:
1357 34 31
BfrBI ATG'CAT
Cuts at: 0 433 1422
Size: 433 989
Bme1580I G_kGCm'C
Cuts at: 0 1049 1221 1422
Size: 1049 172 201
Fragments arranged by size:
1049 201 172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 1101 1422
Size: 281 820 321
Fragments arranged by size:
820 321 281
Bpu10I CC'TnA_GC
Cuts at: 0 1339 1422
Size: 1339 83
BsaI GGTCTCn'nnnn_
Cuts at: 0 1077 1422
Size: 1077 345
BsaAI yAC'GTr
Cuts at: 0 1122 1422
Size: 1122 300
BsaBI GATnn'nnATC
Cuts at: 0 305 592 1422
Size: 305 287 830
Fragments arranged by size:
830 305 287
BsaHI Gr'CG_yC
Cuts at: 0 1257 1422
Size: 1257 165
BsaWI w'CCGG_w
Cuts at: 0 297 1422
Size: 297 1125
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 617 647 1422
Size: 617 30 775
Fragments arranged by size:
775 617 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1384 1422
Size: 1384 38
BseYI C'CCAG_C
Cuts at: 0 242 797 1053 1422
Size: 242 555 256 369
Fragments arranged by size:
555 369 256 242
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 1422
Size: 532 890
BsiHKAI G_wGCw'C
Cuts at: 0 632 1422
Size: 632 790
BsmI GAATG_Cn'
Cuts at: 0 179 435 1422
Size: 179 256 987
Fragments arranged by size:
987 256 179
Bsp1286I G_dGCh'C
Cuts at: 0 107 632 1049 1221 1422
Size: 107 525 417 172 201
Fragments arranged by size:
525 417 201 172 107
BspEI T'CCGG_A
Cuts at: 0 297 1422
Size: 297 1125
BsrBI CCG'CTC
Cuts at: 0 1271 1422
Size: 1271 151
BsrDI GCAATG_nn'
Cuts at: 0 408 1422
Size: 408 1014
BsrFI r'CCGG_y
Cuts at: 0 20 255 1422
Size: 20 235 1167
Fragments arranged by size:
1167 235 20
BssSI C'ACGA_G
Cuts at: 0 631 1422
Size: 631 791
BstAPI GCAn_nnn'nTGC
Cuts at: 0 774 1291 1422
Size: 774 517 131
Fragments arranged by size:
774 517 131
BstEII G'GTnAC_C
Cuts at: 0 540 1422
Size: 540 882
BstYI r'GATC_y
Cuts at: 0 722 1422
Size: 722 700
BtgI C'CryG_G
Cuts at: 0 903 1422
Size: 903 519
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1012 1422
Size: 1012 410
EcoICRI GAG'CTC
Cuts at: 0 630 1422
Size: 630 792
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 445 1036 1101 1422
Size: 281 164 591 65 321
Fragments arranged by size:
591 321 281 164 65
EcoO109I rG'GnC_Cy
Cuts at: 0 642 1422
Size: 642 780
EcoRI G'AATT_C
Cuts at: 0 579 1422
Size: 579 843
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 789 1422
Size: 789 633
HaeII r_GCGC'y
Cuts at: 0 1212 1260 1422
Size: 1212 48 162
Fragments arranged by size:
1212 162 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 985 1017 1116 1148 1396 1422
Size: 985 32 99 32 248 26
Fragments arranged by size:
985 248 99 32 32 26
KasI G'GCGC_C
Cuts at: 0 1256 1422
Size: 1256 166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 410 1115 1289 1422
Size: 118 292 705 174 133
Fragments arranged by size:
705 292 174 133 118
MslI CAynn'nnrTG
Cuts at: 0 487 745 1422
Size: 487 258 677
Fragments arranged by size:
677 487 258
MspA1I CmG'CkG
Cuts at: 0 810 1291 1422
Size: 810 481 131
Fragments arranged by size:
810 481 131
NarI GG'CG_CC
Cuts at: 0 1257 1422
Size: 1257 165
NcoI C'CATG_G
Cuts at: 0 903 1422
Size: 903 519
NdeI CA'TA_TG
Cuts at: 0 435 1422
Size: 435 987
NsiI A_TGCA'T
Cuts at: 0 435 1422
Size: 435 987
NspI r_CATG'y
Cuts at: 0 561 1011 1422
Size: 561 450 411
Fragments arranged by size:
561 450 411
PasI CC'CwG_GG
Cuts at: 0 498 1422
Size: 498 924
PciI A'CATG_T
Cuts at: 0 557 1007 1422
Size: 557 450 415
Fragments arranged by size:
557 450 415
PpuMI rG'GwC_Cy
Cuts at: 0 642 1422
Size: 642 780
PspOMI G'GGCC_C
Cuts at: 0 1217 1422
Size: 1217 205
PstI C_TGCA'G
Cuts at: 0 1287 1422
Size: 1287 135
SacI G_AGCT'C
Cuts at: 0 632 1422
Size: 632 790
SanDI GG'GwC_CC
Cuts at: 0 642 1422
Size: 642 780
SexAI A'CCwGG_T
Cuts at: 0 379 602 1422
Size: 379 223 820
Fragments arranged by size:
820 379 223
SfcI C'TryA_G
Cuts at: 0 1283 1422
Size: 1283 139
SfoI GGC'GCC
Cuts at: 0 1258 1422
Size: 1258 164
StyI C'CwwG_G
Cuts at: 0 195 525 903 1362 1422
Size: 195 330 378 459 60
Fragments arranged by size:
459 378 330 195 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 68 185 1422
Size: 68 117 1237
Fragments arranged by size:
1237 117 68
TatI w'GTAC_w
Cuts at: 0 155 443 680 848 1422
Size: 155 288 237 168 574
Fragments arranged by size:
574 288 237 168 155
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 617 649 699 731 1422
Size: 617 32 50 32 691
Fragments arranged by size:
691 617 50 32 32
Enzymes that do cut:
AcuI AflIII AleI AlfI AlwNI ApaI ApoI BanI
BanII BbeI BbvCI BcgI BfrBI Bme1580I BpmI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspEI BsrBI BsrDI BsrFI
BssSI BstAPI BstEII BstYI BtgI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI HaeII Hin4I KasI MmeI MslI
MspA1I NarI NcoI NdeI NsiI NspI PasI PciI
PpuMI PspOMI PstI SacI SanDI SexAI SfcI SfoI
StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbsI BciVI BclI BglI BglII BlpI
BmgBI BmrI BmtI BplI BpuEI BsiEI BsiWI BsmBI
BspHI BspMI BsrGI BssHII BstBI BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EcoNI EcoRV FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MscI NaeI NgoMIV
NheI NotI NruI PacI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacII SalI SapI SbfI ScaI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI