Restriction map for
pF1KE9407
(Linear) (Six-base) MAPSORT of: KIEE9407.seq Check: 7171 from: 1 to: 999
KIEE9407.orf
With 182 enzymes: SgfI *
November 11, 2015 11:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 999
Size: 310 689
AflIII A'CryG_T
Cuts at: 0 14 16 302 999
Size: 14 2 286 697
Fragments arranged by size:
697 286 14 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 355 999
Size: 355 644
ApoI r'AATT_y
Cuts at: 0 849 999
Size: 849 150
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3 36 999
Size: 3 33 963
Fragments arranged by size:
963 33 3
BbsI GAAGACnn'nnnn_
Cuts at: 0 625 906 999
Size: 625 281 93
Fragments arranged by size:
625 281 93
BclI T'GATC_A
Cuts at: 0 428 999
Size: 428 571
BglI GCCn_nnn'nGGC
Cuts at: 0 258 999
Size: 258 741
BglII A'GATC_T
Cuts at: 0 660 999
Size: 660 339
BmrI ACTGGGnnnn_n'
Cuts at: 0 534 859 999
Size: 534 325 140
Fragments arranged by size:
534 325 140
BsaI GGTCTCn'nnnn_
Cuts at: 0 103 271 999
Size: 103 168 728
Fragments arranged by size:
728 168 103
BsaAI yAC'GTr
Cuts at: 0 17 999
Size: 17 982
BsaBI GATnn'nnATC
Cuts at: 0 644 999
Size: 644 355
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 451 999
Size: 451 548
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 999
Size: 725 274
BspHI T'CATG_A
Cuts at: 0 243 999
Size: 243 756
BsrDI GCAATG_nn'
Cuts at: 0 185 413 999
Size: 185 228 586
Fragments arranged by size:
586 228 185
BsrGI T'GTAC_A
Cuts at: 0 404 999
Size: 404 595
BstXI CCAn_nnnn'nTGG
Cuts at: 0 664 999
Size: 664 335
BstYI r'GATC_y
Cuts at: 0 224 510 660 999
Size: 224 286 150 339
Fragments arranged by size:
339 286 224 150
BtgI C'CryG_G
Cuts at: 0 650 999
Size: 650 349
BtsI GCAGTG_nn'
Cuts at: 0 74 631 999
Size: 74 557 368
Fragments arranged by size:
557 368 74
EaeI y'GGCC_r
Cuts at: 0 250 999
Size: 250 749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 999
Size: 310 689
EcoRI G'AATT_C
Cuts at: 0 849 999
Size: 849 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 466 498 999
Size: 466 32 501
Fragments arranged by size:
501 466 32
HincII GTy'rAC
Cuts at: 0 300 531 999
Size: 300 231 468
Fragments arranged by size:
468 300 231
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 960 999
Size: 960 39
MscI TGG'CCA
Cuts at: 0 252 999
Size: 252 747
MslI CAynn'nnrTG
Cuts at: 0 88 337 999
Size: 88 249 662
Fragments arranged by size:
662 249 88
NcoI C'CATG_G
Cuts at: 0 650 999
Size: 650 349
NspI r_CATG'y
Cuts at: 0 276 306 999
Size: 276 30 693
Fragments arranged by size:
693 276 30
PciI A'CATG_T
Cuts at: 0 302 999
Size: 302 697
PmlI CAC'GTG
Cuts at: 0 17 999
Size: 17 982
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 258 999
Size: 258 741
SspI AAT'ATT
Cuts at: 0 815 999
Size: 815 184
StyI C'CwwG_G
Cuts at: 0 650 999
Size: 650 349
TatI w'GTAC_w
Cuts at: 0 404 447 999
Size: 404 43 552
Fragments arranged by size:
552 404 43
Tth111I GACn'n_nGTC
Cuts at: 0 865 999
Size: 865 134
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 504 999
Size: 504 495
Enzymes that do cut:
AcuI AflIII AlfI ApoI BaeI BbsI BclI BglI
BglII BmrI BsaI BsaAI BsaBI BseRI BsgI BspHI
BsrDI BsrGI BstXI BstYI BtgI BtsI EaeI Eco57MI
EcoRI Hin4I HincII MmeI MscI MslI NcoI NspI
PciI PmlI SfiI SspI StyI TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbvCI
BcgI BciVI BfrBI BlpI Bme1580I BmgBI BmtI BplI
BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI StuI SwaI TaqII TstI XbaI
XhoI XmaI XmnI ZraI