Restriction map for
pF1KE9257
(Linear) (Six-base) MAPSORT of: KIEE9257.seq Check: 570 from: 1 to: 2577
KIEE9257.orf
With 182 enzymes: SgfI *
May 12, 2014 09:13 ..
AatII G_ACGT'C
Cuts at: 0 1793 2577
Size: 1793 784
Acc65I G'GTAC_C
Cuts at: 0 2441 2577
Size: 2441 136
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1420 1543 1714 2036 2495 2577
Size: 1420 123 171 322 459 82
Fragments arranged by size:
1420 459 322 171 123 82
AflIII A'CryG_T
Cuts at: 0 1529 2577
Size: 1529 1048
AleI CACnn'nnGTG
Cuts at: 0 526 2203 2368 2577
Size: 526 1677 165 209
Fragments arranged by size:
1677 526 209 165
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1162 2577
Size: 1162 1415
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 218 250 1495 1527 2577
Size: 218 32 1245 32 1050
Fragments arranged by size:
1245 1050 218 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 483 1677 2577
Size: 483 1194 900
Fragments arranged by size:
1194 900 483
ApaLI G'TGCA_C
Cuts at: 0 915 1383 2577
Size: 915 468 1194
Fragments arranged by size:
1194 915 468
ApoI r'AATT_y
Cuts at: 0 1186 2577
Size: 1186 1391
AvaI C'yCGr_G
Cuts at: 0 6 198 1431 2098 2577
Size: 6 192 1233 667 479
Fragments arranged by size:
1233 667 479 192 6
BanI G'GyrC_C
Cuts at: 0 1113 1517 2129 2441 2577
Size: 1113 404 612 312 136
Fragments arranged by size:
1113 612 404 312 136
BanII G_rGCy'C
Cuts at: 0 1508 1946 2577
Size: 1508 438 631
Fragments arranged by size:
1508 631 438
BbeI G_GCGC'C
Cuts at: 0 1117 2577
Size: 1117 1460
BclI T'GATC_A
Cuts at: 0 1091 2577
Size: 1091 1486
BglI GCCn_nnn'nGGC
Cuts at: 0 1122 2434 2577
Size: 1122 1312 143
Fragments arranged by size:
1312 1122 143
BglII A'GATC_T
Cuts at: 0 254 1427 2577
Size: 254 1173 1150
Fragments arranged by size:
1173 1150 254
Bme1580I G_kGCm'C
Cuts at: 0 199 919 1387 2577
Size: 199 720 468 1190
Fragments arranged by size:
1190 720 468 199
BmgBI CAC'GTC
Cuts at: 0 489 1221 2298 2577
Size: 489 732 1077 279
Fragments arranged by size:
1077 732 489 279
BmrI ACTGGGnnnn_n'
Cuts at: 0 921 1235 2577
Size: 921 314 1342
Fragments arranged by size:
1342 921 314
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 1042 2577
Size: 364 678 1535
Fragments arranged by size:
1535 678 364
BsaI GGTCTCn'nnnn_
Cuts at: 0 2504 2577
Size: 2504 73
BsaAI yAC'GTr
Cuts at: 0 1532 2370 2577
Size: 1532 838 207
Fragments arranged by size:
1532 838 207
BsaHI Gr'CG_yC
Cuts at: 0 1114 1298 1576 1790 1856 2577
Size: 1114 184 278 214 66 721
Fragments arranged by size:
1114 721 278 214 184 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1495 1525 1598 1628 1775 1805 2577
Size: 1495 30 73 30 147 30 772
Fragments arranged by size:
1495 772 147 73 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 216 1936 2313 2577
Size: 216 1720 377 264
Fragments arranged by size:
1720 377 264 216
BseYI C'CCAG_C
Cuts at: 0 454 1373 1617 1710 2397 2577
Size: 454 919 244 93 687 180
Fragments arranged by size:
919 687 454 244 180 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1660 1675 1912 2133 2140 2361 2577
Size: 1660 15 237 221 7 221 216
Fragments arranged by size:
1660 237 221 221 216 15 7
BsiEI CG_ry'CG
Cuts at: 0 1122 2577
Size: 1122 1455
BsiHKAI G_wGCw'C
Cuts at: 0 919 1387 1946 2577
Size: 919 468 559 631
Fragments arranged by size:
919 631 559 468
BsmBI CGTCTCn'nnnn_
Cuts at: 0 463 1430 2577
Size: 463 967 1147
Fragments arranged by size:
1147 967 463
Bsp1286I G_dGCh'C
Cuts at: 0 199 919 1387 1508 1946 2577
Size: 199 720 468 121 438 631
Fragments arranged by size:
720 631 468 438 199 121
BspHI T'CATG_A
Cuts at: 0 494 2577
Size: 494 2083
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2022 2577
Size: 2022 555
BsrBI CCG'CTC
Cuts at: 0 899 2166 2577
Size: 899 1267 411
Fragments arranged by size:
1267 899 411
BsrDI GCAATG_nn'
Cuts at: 0 852 1355 1829 2577
Size: 852 503 474 748
Fragments arranged by size:
852 748 503 474
BsrFI r'CCGG_y
Cuts at: 0 13 1752 1901 2577
Size: 13 1739 149 676
Fragments arranged by size:
1739 676 149 13
BssSI C'ACGA_G
Cuts at: 0 2044 2577
Size: 2044 533
BstAPI GCAn_nnn'nTGC
Cuts at: 0 34 1638 2577
Size: 34 1604 939
Fragments arranged by size:
1604 939 34
BstBI TT'CG_AA
Cuts at: 0 134 2577
Size: 134 2443
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2491 2577
Size: 2491 86
BstYI r'GATC_y
Cuts at: 0 254 1170 1427 2339 2577
Size: 254 916 257 912 238
Fragments arranged by size:
916 912 257 254 238
BtgI C'CryG_G
Cuts at: 0 527 1838 1931 2577
Size: 527 1311 93 646
Fragments arranged by size:
1311 646 527 93
BtsI GCAGTG_nn'
Cuts at: 0 1639 2577
Size: 1639 938
DraI TTT'AAA
Cuts at: 0 246 720 2577
Size: 246 474 1857
Fragments arranged by size:
1857 474 246
DraIII CAC_nnn'GTG
Cuts at: 0 1634 2424 2451 2577
Size: 1634 790 27 126
Fragments arranged by size:
1634 790 126 27
EaeI y'GGCC_r
Cuts at: 0 1625 1925 2054 2525 2577
Size: 1625 300 129 471 52
Fragments arranged by size:
1625 471 300 129 52
EarI CTCTTCn'nnn_
Cuts at: 0 820 1124 2119 2149 2577
Size: 820 304 995 30 428
Fragments arranged by size:
995 820 428 304 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1507 2577
Size: 1507 1070
EcoICRI GAG'CTC
Cuts at: 0 1944 2577
Size: 1944 633
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 1042 1420 1543 1714 2036 2495 2577
Size: 364 678 378 123 171 322 459 82
Fragments arranged by size:
678 459 378 364 322 171 123 82
EcoO109I rG'GnC_Cy
Cuts at: 0 95 480 2577
Size: 95 385 2097
Fragments arranged by size:
2097 385 95
EcoRV GAT'ATC
Cuts at: 0 180 2577
Size: 180 2397
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2300 2577
Size: 2300 277
FspI TGC'GCA
Cuts at: 0 2560 2577
Size: 2560 17
HaeII r_GCGC'y
Cuts at: 0 1117 1380 2404 2577
Size: 1117 263 1024 173
Fragments arranged by size:
1117 1024 263 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1775 1807 1996 2028 2577
Size: 1775 32 189 32 549
Fragments arranged by size:
1775 549 189 32 32
HincII GTy'rAC
Cuts at: 0 210 2577
Size: 210 2367
HpaI GTT'AAC
Cuts at: 0 210 2577
Size: 210 2367
KasI G'GCGC_C
Cuts at: 0 1113 2577
Size: 1113 1464
KpnI G_GTAC'C
Cuts at: 0 2445 2577
Size: 2445 132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 969 1706 2577
Size: 969 737 871
Fragments arranged by size:
969 871 737
MscI TGG'CCA
Cuts at: 0 1627 2056 2527 2577
Size: 1627 429 471 50
Fragments arranged by size:
1627 471 429 50
MslI CAynn'nnrTG
Cuts at: 0 307 526 2116 2203 2368 2468 2577
Size: 307 219 1590 87 165 100 109
Fragments arranged by size:
1590 307 219 165 109 100 87
MspA1I CmG'CkG
Cuts at: 0 885 1548 2355 2577
Size: 885 663 807 222
Fragments arranged by size:
885 807 663 222
NaeI GCC'GGC
Cuts at: 0 15 2577
Size: 15 2562
NarI GG'CG_CC
Cuts at: 0 1114 2577
Size: 1114 1463
NcoI C'CATG_G
Cuts at: 0 1931 2577
Size: 1931 646
NgoMIV G'CCGG_C
Cuts at: 0 13 2577
Size: 13 2564
NspI r_CATG'y
Cuts at: 0 1311 1449 2577
Size: 1311 138 1128
Fragments arranged by size:
1311 1128 138
PasI CC'CwG_GG
Cuts at: 0 1740 2577
Size: 1740 837
PflMI CCAn_nnn'nTGG
Cuts at: 0 398 483 2451 2523 2577
Size: 398 85 1968 72 54
Fragments arranged by size:
1968 398 85 72 54
PfoI T'CCnGG_A
Cuts at: 0 100 2577
Size: 100 2477
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1495 1527 2577
Size: 1495 32 1050
Fragments arranged by size:
1495 1050 32
PpuMI rG'GwC_Cy
Cuts at: 0 95 2577
Size: 95 2482
PshAI GACnn'nnGTC
Cuts at: 0 1288 2577
Size: 1288 1289
PstI C_TGCA'G
Cuts at: 0 890 1241 1547 2339 2577
Size: 890 351 306 792 238
Fragments arranged by size:
890 792 351 306 238
PvuI CG_AT'CG
Cuts at: 0 1122 2577
Size: 1122 1455
PvuII CAG'CTG
Cuts at: 0 1548 2355 2577
Size: 1548 807 222
Fragments arranged by size:
1548 807 222
SacI G_AGCT'C
Cuts at: 0 1946 2577
Size: 1946 631
SapI GCTCTTCn'nnn_
Cuts at: 0 2149 2577
Size: 2149 428
SexAI A'CCwGG_T
Cuts at: 0 2420 2447 2577
Size: 2420 27 130
Fragments arranged by size:
2420 130 27
SfcI C'TryA_G
Cuts at: 0 886 1237 1543 2335 2577
Size: 886 351 306 792 242
Fragments arranged by size:
886 792 351 306 242
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2434 2577
Size: 2434 143
SfoI GGC'GCC
Cuts at: 0 1115 2577
Size: 1115 1462
SgrAI Cr'CCGG_yG
Cuts at: 0 1752 2577
Size: 1752 825
SmaI CCC'GGG
Cuts at: 0 2100 2577
Size: 2100 477
SmlI C'TyrA_G
Cuts at: 0 1431 2577
Size: 1431 1146
SphI G_CATG'C
Cuts at: 0 1449 2577
Size: 1449 1128
SspI AAT'ATT
Cuts at: 0 648 2577
Size: 648 1929
StuI AGG'CCT
Cuts at: 0 2068 2283 2577
Size: 2068 215 294
Fragments arranged by size:
2068 294 215
StyI C'CwwG_G
Cuts at: 0 54 1931 2528 2577
Size: 54 1877 597 49
Fragments arranged by size:
1877 597 54 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 390 2236 2577
Size: 390 1846 341
Fragments arranged by size:
1846 390 341
TatI w'GTAC_w
Cuts at: 0 2 2567 2577
Size: 2 2565 10
Fragments arranged by size:
2565 10 2
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 493 525 822 854 999 1031 1598 1630
Size: 493 32 297 32 145 32 567 32
Cuts at: 1630 2577
Size: 947
Fragments arranged by size:
947 567 493 297 145 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 490 1677 2008 2577
Size: 490 1187 331 569
Fragments arranged by size:
1187 569 490 331
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1836 2445 2577
Size: 1836 609 132
Fragments arranged by size:
1836 609 132
XhoI C'TCGA_G
Cuts at: 0 1431 2577
Size: 1431 1146
XmaI C'CCGG_G
Cuts at: 0 2098 2577
Size: 2098 479
XmnI GAAnn'nnTTC
Cuts at: 0 1405 2577
Size: 1405 1172
ZraI GAC'GTC
Cuts at: 0 1791 2577
Size: 1791 786
Enzymes that do cut:
AatII Acc65I AcuI AflIII AleI AlfI AloI AlwNI
ApaLI ApoI AvaI BanI BanII BbeI BclI BglI
BglII Bme1580I BmgBI BmrI BpmI BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtsI DraI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRV FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI
PfoI PpiI PpuMI PshAI PstI PvuI PvuII SacI
SapI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AgeI AhdI ApaI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BcgI BciVI BfrBI BlpI BmtI BplI Bpu10I BpuEI
BsaBI BsaWI BsiWI BsmI BspEI BsrGI BssHII BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DrdI EagI EcoNI
EcoRI FseI FspAI HindIII MfeI MluI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PsiI
PspOMI PspXI PsrI RsrII SacII SalI SanDI SbfI
ScaI SgfI SnaBI SpeI SrfI SwaI XbaI