Restriction map for
pF1KE6669
(Linear) (Six-base) MAPSORT of: KIEE6669.seq Check: 5413 from: 1 to: 888
KIEE6669.orf
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 608 888
Size: 608 280
AlwNI CAG_nnn'CTG
Cuts at: 0 327 888
Size: 327 561
ApoI r'AATT_y
Cuts at: 0 394 888
Size: 394 494
AvaI C'yCGr_G
Cuts at: 0 13 142 888
Size: 13 129 746
Fragments arranged by size:
746 129 13
BanII G_rGCy'C
Cuts at: 0 14 143 232 888
Size: 14 129 89 656
Fragments arranged by size:
656 129 89 14
BbvCI CC'TCA_GC
Cuts at: 0 99 888
Size: 99 789
BglI GCCn_nnn'nGGC
Cuts at: 0 56 888
Size: 56 832
BlpI GC'TnA_GC
Cuts at: 0 341 888
Size: 341 547
BmgBI CAC'GTC
Cuts at: 0 279 888
Size: 279 609
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 634 888
Size: 216 418 254
Fragments arranged by size:
418 254 216
Bpu10I CC'TnA_GC
Cuts at: 0 99 320 888
Size: 99 221 568
Fragments arranged by size:
568 221 99
BsaI GGTCTCn'nnnn_
Cuts at: 0 534 888
Size: 534 354
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 382 439 888
Size: 382 57 449
Fragments arranged by size:
449 382 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 888
Size: 820 68
BsiEI CG_ry'CG
Cuts at: 0 317 888
Size: 317 571
BsiHKAI G_wGCw'C
Cuts at: 0 143 232 888
Size: 143 89 656
Fragments arranged by size:
656 143 89
BsmI GAATG_Cn'
Cuts at: 0 9 496 888
Size: 9 487 392
Fragments arranged by size:
487 392 9
Bsp1286I G_dGCh'C
Cuts at: 0 14 143 232 888
Size: 14 129 89 656
Fragments arranged by size:
656 129 89 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 440 888
Size: 440 448
BsrBI CCG'CTC
Cuts at: 0 319 480 888
Size: 319 161 408
Fragments arranged by size:
408 319 161
BsrFI r'CCGG_y
Cuts at: 0 55 888
Size: 55 833
BssSI C'ACGA_G
Cuts at: 0 364 888
Size: 364 524
BstXI CCAn_nnnn'nTGG
Cuts at: 0 641 888
Size: 641 247
BstYI r'GATC_y
Cuts at: 0 860 888
Size: 860 28
BtsI GCAGTG_nn'
Cuts at: 0 228 837 888
Size: 228 609 51
Fragments arranged by size:
609 228 51
EaeI y'GGCC_r
Cuts at: 0 48 314 888
Size: 48 266 574
Fragments arranged by size:
574 266 48
EagI C'GGCC_G
Cuts at: 0 314 888
Size: 314 574
EarI CTCTTCn'nnn_
Cuts at: 0 457 470 566 851 888
Size: 457 13 96 285 37
Fragments arranged by size:
457 285 96 37 13
EcoICRI GAG'CTC
Cuts at: 0 141 230 888
Size: 141 89 658
Fragments arranged by size:
658 141 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 634 888
Size: 216 418 254
Fragments arranged by size:
418 254 216
EcoO109I rG'GnC_Cy
Cuts at: 0 658 707 888
Size: 658 49 181
Fragments arranged by size:
658 181 49
EcoRI G'AATT_C
Cuts at: 0 394 888
Size: 394 494
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 594 626 888
Size: 594 32 262
Fragments arranged by size:
594 262 32
HindIII A'AGCT_T
Cuts at: 0 687 888
Size: 687 201
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 888
Size: 449 439
MslI CAynn'nnrTG
Cuts at: 0 639 888
Size: 639 249
MspA1I CmG'CkG
Cuts at: 0 460 503 888
Size: 460 43 385
Fragments arranged by size:
460 385 43
NaeI GCC'GGC
Cuts at: 0 57 888
Size: 57 831
NgoMIV G'CCGG_C
Cuts at: 0 55 888
Size: 55 833
NotI GC'GGCC_GC
Cuts at: 0 314 888
Size: 314 574
NspI r_CATG'y
Cuts at: 0 376 888
Size: 376 512
PflMI CCAn_nnn'nTGG
Cuts at: 0 201 888
Size: 201 687
PpuMI rG'GwC_Cy
Cuts at: 0 658 707 888
Size: 658 49 181
Fragments arranged by size:
658 181 49
PstI C_TGCA'G
Cuts at: 0 223 888
Size: 223 665
PvuII CAG'CTG
Cuts at: 0 460 888
Size: 460 428
SacI G_AGCT'C
Cuts at: 0 143 232 888
Size: 143 89 656
Fragments arranged by size:
656 143 89
SapI GCTCTTCn'nnn_
Cuts at: 0 470 566 888
Size: 470 96 322
Fragments arranged by size:
470 322 96
SexAI A'CCwGG_T
Cuts at: 0 660 888
Size: 660 228
SfcI C'TryA_G
Cuts at: 0 219 888
Size: 219 669
SspI AAT'ATT
Cuts at: 0 871 888
Size: 871 17
StyI C'CwwG_G
Cuts at: 0 710 888
Size: 710 178
Tth111I GACn'n_nGTC
Cuts at: 0 303 662 888
Size: 303 359 226
Fragments arranged by size:
359 303 226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 198 700 888
Size: 198 502 188
Fragments arranged by size:
502 198 188
Enzymes that do cut:
AhdI AlwNI ApoI AvaI BanII BbvCI BglI BlpI
BmgBI BpmI Bpu10I BsaI BseRI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrFI BssSI BstXI BstYI
BtsI EaeI EagI EarI EcoICRI Eco57MI EcoO109I EcoRI
Hin4I HindIII MmeI MslI MspA1I NaeI NgoMIV NotI
NspI PflMI PpuMI PstI PvuII SacI SapI SexAI
SfcI SspI StyI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BcgI BciVI BclI BfrBI BglII Bme1580I BmrI BmtI
BplI BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EciI EcoNI EcoRV FalI FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MscI NarI NcoI NdeI NheI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StuI SwaI TaqII
TatI TstI XbaI XhoI XmaI XmnI ZraI