Restriction map for
pF1KE5665
(Linear) (Six-base) MAPSORT of: KIEE5665.seq Check: 1889 from: 1 to: 1584
KIEE5665.orf
With 182 enzymes: SgfI *
October 12, 2018 16:45 ..
AflIII A'CryG_T
Cuts at: 0 674 1220 1253 1584
Size: 674 546 33 331
Fragments arranged by size:
674 546 331 33
AleI CACnn'nnGTG
Cuts at: 0 505 782 1584
Size: 505 277 802
Fragments arranged by size:
802 505 277
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 800 1538 1584
Size: 800 738 46
Fragments arranged by size:
800 738 46
AlwNI CAG_nnn'CTG
Cuts at: 0 571 700 937 1584
Size: 571 129 237 647
Fragments arranged by size:
647 571 237 129
ApaI G_GGCC'C
Cuts at: 0 11 1584
Size: 11 1573
AvaI C'yCGr_G
Cuts at: 0 1245 1584
Size: 1245 339
BamHI G'GATC_C
Cuts at: 0 215 1584
Size: 215 1369
BanI G'GyrC_C
Cuts at: 0 192 321 529 602 784 1329 1485 1584
Size: 192 129 208 73 182 545 156 99
Fragments arranged by size:
545 208 192 182 156 129 99 73
BanII G_rGCy'C
Cuts at: 0 11 815 869 1174 1306 1466 1584
Size: 11 804 54 305 132 160 118
Fragments arranged by size:
804 305 160 132 118 54 11
BbeI G_GCGC'C
Cuts at: 0 1489 1584
Size: 1489 95
BbsI GAAGACnn'nnnn_
Cuts at: 0 1131 1367 1584
Size: 1131 236 217
Fragments arranged by size:
1131 236 217
BbvCI CC'TCA_GC
Cuts at: 0 68 107 585 1584
Size: 68 39 478 999
Fragments arranged by size:
999 478 68 39
BlpI GC'TnA_GC
Cuts at: 0 952 1584
Size: 952 632
Bme1580I G_kGCm'C
Cuts at: 0 11 197 1334 1584
Size: 11 186 1137 250
Fragments arranged by size:
1137 250 186 11
BmgBI CAC'GTC
Cuts at: 0 648 1223 1281 1365 1584
Size: 648 575 58 84 219
Fragments arranged by size:
648 575 219 84 58
BmrI ACTGGGnnnn_n'
Cuts at: 0 253 1513 1584
Size: 253 1260 71
Fragments arranged by size:
1260 253 71
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 462 1584
Size: 462 1122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1480 1584
Size: 1480 104
Bpu10I CC'TnA_GC
Cuts at: 0 68 107 585 1202 1584
Size: 68 39 478 617 382
Fragments arranged by size:
617 478 382 68 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 1584
Size: 411 1173
BsaI GGTCTCn'nnnn_
Cuts at: 0 890 1339 1584
Size: 890 449 245
Fragments arranged by size:
890 449 245
BsaAI yAC'GTr
Cuts at: 0 1254 1584
Size: 1254 330
BsaHI Gr'CG_yC
Cuts at: 0 56 256 823 1064 1486 1508 1570 1584
Size: 56 200 567 241 422 22 62 14
Fragments arranged by size:
567 422 241 200 62 56 22 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 88 1111 1584
Size: 85 3 1023 473
Fragments arranged by size:
1023 473 85 3
BseYI C'CCAG_C
Cuts at: 0 27 63 708 1192 1563 1584
Size: 27 36 645 484 371 21
Fragments arranged by size:
645 484 371 36 27 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 529 778 1432 1482 1584
Size: 416 113 249 654 50 102
Fragments arranged by size:
654 416 249 113 102 50
BsiEI CG_ry'CG
Cuts at: 0 996 1584
Size: 996 588
BsiHKAI G_wGCw'C
Cuts at: 0 815 1174 1584
Size: 815 359 410
Fragments arranged by size:
815 410 359
BsmBI CGTCTCn'nnnn_
Cuts at: 0 247 1584
Size: 247 1337
Bsp1286I G_dGCh'C
Cuts at: 0 11 197 815 869 1174 1306 1334 1466
Size: 11 186 618 54 305 132 28 132
Cuts at: 1466 1584
Size: 118
Fragments arranged by size:
618 305 186 132 132 118 54 28
11
BspHI T'CATG_A
Cuts at: 0 1014 1584
Size: 1014 570
BsrBI CCG'CTC
Cuts at: 0 1298 1584
Size: 1298 286
BsrDI GCAATG_nn'
Cuts at: 0 441 1245 1584
Size: 441 804 339
Fragments arranged by size:
804 441 339
BsrFI r'CCGG_y
Cuts at: 0 526 1584
Size: 526 1058
BssHII G'CGCG_C
Cuts at: 0 1378 1584
Size: 1378 206
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1401 1410 1584
Size: 1401 9 174
Fragments arranged by size:
1401 174 9
BstEII G'GTnAC_C
Cuts at: 0 492 1584
Size: 492 1092
BstYI r'GATC_y
Cuts at: 0 215 887 1584
Size: 215 672 697
Fragments arranged by size:
697 672 215
Bsu36I CC'TnA_GG
Cuts at: 0 34 1584
Size: 34 1550
BtgI C'CryG_G
Cuts at: 0 197 266 344 683 966 1005 1350 1584
Size: 197 69 78 339 283 39 345 234
Fragments arranged by size:
345 339 283 234 197 78 69 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 194 597 1584
Size: 194 403 987
Fragments arranged by size:
987 403 194
BtsI GCAGTG_nn'
Cuts at: 0 1537 1584
Size: 1537 47
DraIII CAC_nnn'GTG
Cuts at: 0 546 1401 1584
Size: 546 855 183
Fragments arranged by size:
855 546 183
EaeI y'GGCC_r
Cuts at: 0 263 993 1544 1584
Size: 263 730 551 40
Fragments arranged by size:
730 551 263 40
EagI C'GGCC_G
Cuts at: 0 993 1584
Size: 993 591
EarI CTCTTCn'nnn_
Cuts at: 0 175 1584
Size: 175 1409
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 674 1584
Size: 674 910
EcoICRI GAG'CTC
Cuts at: 0 813 1172 1584
Size: 813 359 412
Fragments arranged by size:
813 412 359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1480 1584
Size: 1480 104
EcoNI CCTnn'n_nnAGG
Cuts at: 0 757 1584
Size: 757 827
EcoO109I rG'GnC_Cy
Cuts at: 0 7 8 111 247 689 1550 1584
Size: 7 1 103 136 442 861 34
Fragments arranged by size:
861 442 136 103 34 7 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 390 1584
Size: 390 1194
HaeII r_GCGC'y
Cuts at: 0 1489 1584
Size: 1489 95
KasI G'GCGC_C
Cuts at: 0 1485 1584
Size: 1485 99
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 1584
Size: 656 928
MscI TGG'CCA
Cuts at: 0 265 1584
Size: 265 1319
MslI CAynn'nnrTG
Cuts at: 0 448 505 646 782 1019 1133 1584
Size: 448 57 141 136 237 114 451
Fragments arranged by size:
451 448 237 141 136 114 57
MspA1I CmG'CkG
Cuts at: 0 67 106 712 968 1123 1576 1584
Size: 67 39 606 256 155 453 8
Fragments arranged by size:
606 453 256 155 67 39 8
NaeI GCC'GGC
Cuts at: 0 528 1584
Size: 528 1056
NarI GG'CG_CC
Cuts at: 0 1486 1584
Size: 1486 98
NcoI C'CATG_G
Cuts at: 0 266 683 1005 1584
Size: 266 417 322 579
Fragments arranged by size:
579 417 322 266
NgoMIV G'CCGG_C
Cuts at: 0 526 1584
Size: 526 1058
NotI GC'GGCC_GC
Cuts at: 0 993 1584
Size: 993 591
NspI r_CATG'y
Cuts at: 0 579 678 1438 1584
Size: 579 99 760 146
Fragments arranged by size:
760 579 146 99
PasI CC'CwG_GG
Cuts at: 0 11 51 1584
Size: 11 40 1533
Fragments arranged by size:
1533 40 11
PciI A'CATG_T
Cuts at: 0 674 1584
Size: 674 910
PflMI CCAn_nnn'nTGG
Cuts at: 0 852 1584
Size: 852 732
PpuMI rG'GwC_Cy
Cuts at: 0 689 1550 1584
Size: 689 861 34
Fragments arranged by size:
861 689 34
PshAI GACnn'nnGTC
Cuts at: 0 1007 1114 1584
Size: 1007 107 470
Fragments arranged by size:
1007 470 107
PspOMI G'GGCC_C
Cuts at: 0 7 1584
Size: 7 1577
PstI C_TGCA'G
Cuts at: 0 137 1584
Size: 137 1447
PvuII CAG'CTG
Cuts at: 0 67 106 712 1584
Size: 67 39 606 872
Fragments arranged by size:
872 606 67 39
SacI G_AGCT'C
Cuts at: 0 815 1174 1584
Size: 815 359 410
Fragments arranged by size:
815 410 359
SacII CC_GC'GG
Cuts at: 0 969 1584
Size: 969 615
SanDI GG'GwC_CC
Cuts at: 0 689 1550 1584
Size: 689 861 34
Fragments arranged by size:
861 689 34
SexAI A'CCwGG_T
Cuts at: 0 752 1397 1584
Size: 752 645 187
Fragments arranged by size:
752 645 187
SfcI C'TryA_G
Cuts at: 0 40 133 1584
Size: 40 93 1451
Fragments arranged by size:
1451 93 40
SfoI GGC'GCC
Cuts at: 0 1487 1584
Size: 1487 97
SmaI CCC'GGG
Cuts at: 0 1247 1584
Size: 1247 337
SmlI C'TyrA_G
Cuts at: 0 390 1584
Size: 390 1194
SrfI GCCC'GGGC
Cuts at: 0 1247 1584
Size: 1247 337
StyI C'CwwG_G
Cuts at: 0 266 654 683 1005 1584
Size: 266 388 29 322 579
Fragments arranged by size:
579 388 322 266 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3 1532 1584
Size: 3 1529 52
Fragments arranged by size:
1529 52 3
TatI w'GTAC_w
Cuts at: 0 671 1584
Size: 671 913
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1182 1584
Size: 1182 402
XmaI C'CCGG_G
Cuts at: 0 1245 1584
Size: 1245 339
Enzymes that do cut:
AflIII AleI AlfI AlwNI ApaI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BsrBI
BsrDI BsrFI BssHII BstAPI BstEII BstYI Bsu36I BtgI
BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PasI PciI PflMI PpuMI PshAI PspOMI PstI PvuII
SacI SacII SanDI SexAI SfcI SfoI SmaI SmlI
SrfI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BcgI BciVI BclI BfrBI BglI BglII
BmtI BsaBI BsaWI BsaXI BsiWI BsmI BspEI BspMI
BsrGI BssSI BstBI BstXI BstZ17I ClaI CspCI DraI
DrdI EcoRI EcoRV FseI FspI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI NdeI NheI NruI
NsiI PacI PfoI PmeI PmlI PpiI PsiI PspXI
PsrI PvuI RsrII SalI SapI SbfI ScaI SfiI
SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI
TstI Tth111I XbaI XhoI XmnI ZraI