(Linear) (Six-base) MAPSORT of: KIEE5606.seq  Check: 7057  from: 1  to: 1533



KIEE5606.orf

 With 182 enzymes: SgfI * 

                        February 27, 2017 10:28  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1514    1533 
   Size:       1514      19
 
AfeI AGC'GCT

Cuts at:      0     920    1533 
   Size:        920     613
 
AflIII A'CryG_T

Cuts at:      0     647    1533 
   Size:        647     886
 
AlwNI CAG_nnn'CTG

Cuts at:      0     386     704    1533 
   Size:        386     318     829

  Fragments arranged by size: 

                 829     386     318
 
ApoI r'AATT_y

Cuts at:      0     277     808    1442    1533 
   Size:        277     531     634      91

  Fragments arranged by size: 

                 634     531     277      91
 
AseI AT'TA_AT

Cuts at:      0     341    1160    1418    1533 
   Size:        341     819     258     115

  Fragments arranged by size: 

                 819     341     258     115
 
BanI G'GyrC_C

Cuts at:      0    1208    1533 
   Size:       1208     325
 
BanII G_rGCy'C

Cuts at:      0      45    1533 
   Size:         45    1488
 
BbeI G_GCGC'C

Cuts at:      0    1212    1533 
   Size:       1212     321
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     529    1366    1533 
   Size:        529     837     167

  Fragments arranged by size: 

                 837     529     167
 
BglII A'GATC_T

Cuts at:      0     259    1533 
   Size:        259    1274
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     891    1197    1533 
   Size:        891     306     336

  Fragments arranged by size: 

                 891     336     306
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     574     677     986    1427    1533 
   Size:        574     103     309     441     106

  Fragments arranged by size: 

                 574     441     309     106     103
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     174    1533 
   Size:        174    1359
 
BsaAI yAC'GTr

Cuts at:      0     567    1533 
   Size:        567     966
 
BsaHI Gr'CG_yC

Cuts at:      0    1209    1533 
   Size:       1209     324
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     983    1533 
   Size:        983     550
 
BseYI C'CCAG_C

Cuts at:      0      38    1533 
   Size:         38    1495
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     389    1533 
   Size:        389    1144
 
Bsp1286I G_dGCh'C

Cuts at:      0      45    1533 
   Size:         45    1488
 
BsrDI GCAATG_nn'

Cuts at:      0    1188    1245    1533 
   Size:       1188      57     288

  Fragments arranged by size: 

                1188     288      57
 
BsrGI T'GTAC_A

Cuts at:      0    1004    1533 
   Size:       1004     529
 
BssSI C'ACGA_G

Cuts at:      0    1052    1533 
   Size:       1052     481
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1516    1533 
   Size:       1516      17
 
BstYI r'GATC_y

Cuts at:      0     259    1497    1533 
   Size:        259    1238      36

  Fragments arranged by size: 

                1238     259      36
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     117    1533 
   Size:        117    1416
 
BtsI GCAGTG_nn'

Cuts at:      0     783    1533 
   Size:        783     750
 
ClaI AT'CG_AT

Cuts at:      0    1203    1533 
   Size:       1203     330
 
EaeI y'GGCC_r

Cuts at:      0     795    1075    1251    1533 
   Size:        795     280     176     282

  Fragments arranged by size: 

                 795     282     280     176
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     574     677     986    1427    1514    1533 
   Size:        574     103     309     441      87      19

  Fragments arranged by size: 

                 574     441     309     103      87      19
 
EcoRI G'AATT_C

Cuts at:      0     808    1533 
   Size:        808     725
 
EcoRV GAT'ATC

Cuts at:      0     486    1533 
   Size:        486    1047
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     743    1533 
   Size:        743     790
 
HaeII r_GCGC'y

Cuts at:      0     922    1212    1533 
   Size:        922     290     321

  Fragments arranged by size: 

                 922     321     290
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     850     882     908     940    1330    1362    1533 
   Size:        850      32      26      32     390      32     171

  Fragments arranged by size: 

                 850     390     171      32      32      32      26
 
KasI G'GCGC_C

Cuts at:      0    1208    1533 
   Size:       1208     325
 
MfeI C'AATT_G

Cuts at:      0    1425    1533 
   Size:       1425     108
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     999    1533 
   Size:        999     534
 
MscI TGG'CCA

Cuts at:      0    1077    1253    1533 
   Size:       1077     176     280

  Fragments arranged by size: 

                1077     280     176
 
MslI CAynn'nnrTG

Cuts at:      0     350    1533 
   Size:        350    1183
 
NarI GG'CG_CC

Cuts at:      0    1209    1533 
   Size:       1209     324
 
NdeI CA'TA_TG

Cuts at:      0     347    1533 
   Size:        347    1186
 
NspI r_CATG'y

Cuts at:      0     651    1533 
   Size:        651     882
 
PciI A'CATG_T

Cuts at:      0     647    1533 
   Size:        647     886
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     439    1533 
   Size:        439    1094
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     366     398    1533 
   Size:        366      32    1135

  Fragments arranged by size: 

                1135     366      32
 
PstI C_TGCA'G

Cuts at:      0     717    1533 
   Size:        717     816
 
SbfI CC_TGCA'GG

Cuts at:      0     717    1533 
   Size:        717     816
 
SfcI C'TryA_G

Cuts at:      0     713    1533 
   Size:        713     820
 
SfoI GGC'GCC

Cuts at:      0    1210    1533 
   Size:       1210     323
 
StyI C'CwwG_G

Cuts at:      0     214    1533 
   Size:        214    1319
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     745    1533 
   Size:        745     788
 
TatI w'GTAC_w

Cuts at:      0    1004    1533 
   Size:       1004     529
 
Tth111I GACn'n_nGTC

Cuts at:      0    1189    1533 
   Size:       1189     344
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     440    1297    1533 
   Size:        440     857     236

  Fragments arranged by size: 

                 857     440     236
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII    AlwNI     ApoI     AseI     BanI    BanII
     BbeI     BbsI    BglII     BmrI     BpmI     BsaI    BsaAI    BsaHI
    BseRI    BseYI     BsgI Bsp1286I    BsrDI    BsrGI    BssSI   BstAPI
    BstYI    BtgZI     BtsI     ClaI     EaeI  Eco57MI    EcoRI    EcoRV
     FalI    HaeII    Hin4I     KasI     MfeI     MmeI     MscI     MslI
     NarI     NdeI     NspI     PciI    PflMI     PsrI     PstI     SbfI
     SfcI     SfoI     StyI    TaqII     TatI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI
    BsaBI    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MluI   MspA1I     NaeI
     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI
     XbaI     XhoI     XmaI     XmnI     ZraI