Restriction map for
pF1KE4516
(Linear) (Six-base) MAPSORT of: KIEE4516.seq Check: 6490 from: 1 to: 1650
KIEE4516.orf
With 182 enzymes: SgfI *
December 8, 2011 13:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 15 755 1650
Size: 15 740 895
Fragments arranged by size:
895 740 15
AccI GT'mk_AC
Cuts at: 0 686 1330 1650
Size: 686 644 320
Fragments arranged by size:
686 644 320
AfeI AGC'GCT
Cuts at: 0 1494 1650
Size: 1494 156
AleI CACnn'nnGTG
Cuts at: 0 67 1650
Size: 67 1583
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1236 1650
Size: 1236 414
AlwNI CAG_nnn'CTG
Cuts at: 0 756 1533 1554 1650
Size: 756 777 21 96
Fragments arranged by size:
777 756 96 21
ApaI G_GGCC'C
Cuts at: 0 396 861 1650
Size: 396 465 789
Fragments arranged by size:
789 465 396
AvaI C'yCGr_G
Cuts at: 0 203 582 1394 1650
Size: 203 379 812 256
Fragments arranged by size:
812 379 256 203
BanI G'GyrC_C
Cuts at: 0 667 740 1650
Size: 667 73 910
Fragments arranged by size:
910 667 73
BanII G_rGCy'C
Cuts at: 0 311 396 861 1417 1442 1650
Size: 311 85 465 556 25 208
Fragments arranged by size:
556 465 311 208 85 25
BbeI G_GCGC'C
Cuts at: 0 744 1650
Size: 744 906
BbvCI CC'TCA_GC
Cuts at: 0 169 1626 1650
Size: 169 1457 24
Fragments arranged by size:
1457 169 24
BciVI GTATCCnnnnn_n'
Cuts at: 0 1355 1650
Size: 1355 295
BglI GCCn_nnn'nGGC
Cuts at: 0 738 1195 1650
Size: 738 457 455
Fragments arranged by size:
738 457 455
Bme1580I G_kGCm'C
Cuts at: 0 396 670 734 861 1650
Size: 396 274 64 127 789
Fragments arranged by size:
789 396 274 127 64
BmrI ACTGGGnnnn_n'
Cuts at: 0 405 1343 1650
Size: 405 938 307
Fragments arranged by size:
938 405 307
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 1576 1650
Size: 208 1368 74
Fragments arranged by size:
1368 208 74
Bpu10I CC'TnA_GC
Cuts at: 0 169 1626 1650
Size: 169 1457 24
Fragments arranged by size:
1457 169 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 765 1510 1650
Size: 765 745 140
Fragments arranged by size:
765 745 140
BsaHI Gr'CG_yC
Cuts at: 0 741 1650
Size: 741 909
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 434 464 814 844 1650
Size: 434 30 350 30 806
Fragments arranged by size:
806 434 350 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 568 816 819 1650
Size: 568 248 3 831
Fragments arranged by size:
831 568 248 3
BseYI C'CCAG_C
Cuts at: 0 1284 1650
Size: 1284 366
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 1184 1567 1650
Size: 297 887 383 83
Fragments arranged by size:
887 383 297 83
BsiEI CG_ry'CG
Cuts at: 0 42 1650
Size: 42 1608
BsiHKAI G_wGCw'C
Cuts at: 0 385 1442 1598 1650
Size: 385 1057 156 52
Fragments arranged by size:
1057 385 156 52
Bsp1286I G_dGCh'C
Cuts at: 0 311 385 396 670 734 861 1417 1442
Size: 311 74 11 274 64 127 556 25
Cuts at: 1442 1598 1650
Size: 156 52
Fragments arranged by size:
556 311 274 156 127 74 64 52
25 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 15 515 538 755 1085 1142 1650
Size: 15 500 23 217 330 57 508
Fragments arranged by size:
508 500 330 217 57 23 15
BsrFI r'CCGG_y
Cuts at: 0 1181 1650
Size: 1181 469
BsrGI T'GTAC_A
Cuts at: 0 419 1650
Size: 419 1231
BstEII G'GTnAC_C
Cuts at: 0 60 1650
Size: 60 1590
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1149 1650
Size: 1149 501
BstYI r'GATC_y
Cuts at: 0 53 326 1650
Size: 53 273 1324
Fragments arranged by size:
1324 273 53
BstZ17I GTA'TAC
Cuts at: 0 1331 1650
Size: 1331 319
Bsu36I CC'TnA_GG
Cuts at: 0 853 1650
Size: 853 797
BtgI C'CryG_G
Cuts at: 0 446 953 977 1388 1650
Size: 446 507 24 411 262
Fragments arranged by size:
507 446 411 262 24
BtsI GCAGTG_nn'
Cuts at: 0 119 141 259 1201 1531 1615 1650
Size: 119 22 118 942 330 84 35
Fragments arranged by size:
942 330 119 118 84 35 22
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1032 1067 1650
Size: 1032 35 583
Fragments arranged by size:
1032 583 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 519 1650
Size: 519 1131
EaeI y'GGCC_r
Cuts at: 0 39 974 1484 1650
Size: 39 935 510 166
Fragments arranged by size:
935 510 166 39
EagI C'GGCC_G
Cuts at: 0 39 1650
Size: 39 1611
EarI CTCTTCn'nnn_
Cuts at: 0 1018 1465 1650
Size: 1018 447 185
Fragments arranged by size:
1018 447 185
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 994 1650
Size: 994 656
EcoICRI GAG'CTC
Cuts at: 0 1440 1650
Size: 1440 210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 1576 1650
Size: 208 1368 74
Fragments arranged by size:
1368 208 74
EcoNI CCTnn'n_nnAGG
Cuts at: 0 22 834 1108 1650
Size: 22 812 274 542
Fragments arranged by size:
812 542 274 22
EcoO109I rG'GnC_Cy
Cuts at: 0 372 392 393 527 857 858 1551 1650
Size: 372 20 1 134 330 1 693 99
Fragments arranged by size:
693 372 330 134 99 20 1 1
FspI TGC'GCA
Cuts at: 0 534 1650
Size: 534 1116
HaeII r_GCGC'y
Cuts at: 0 744 1496 1650
Size: 744 752 154
Fragments arranged by size:
752 744 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 81 113 1082 1114 1650
Size: 81 32 969 32 536
Fragments arranged by size:
969 536 81 32 32
HindIII A'AGCT_T
Cuts at: 0 1144 1650
Size: 1144 506
KasI G'GCGC_C
Cuts at: 0 740 1650
Size: 740 910
MslI CAynn'nnrTG
Cuts at: 0 67 1375 1650
Size: 67 1308 275
Fragments arranged by size:
1308 275 67
MspA1I CmG'CkG
Cuts at: 0 405 517 818 1650
Size: 405 112 301 832
Fragments arranged by size:
832 405 301 112
NaeI GCC'GGC
Cuts at: 0 1183 1650
Size: 1183 467
NarI GG'CG_CC
Cuts at: 0 741 1650
Size: 741 909
NcoI C'CATG_G
Cuts at: 0 446 953 977 1388 1650
Size: 446 507 24 411 262
Fragments arranged by size:
507 446 411 262 24
NgoMIV G'CCGG_C
Cuts at: 0 1181 1650
Size: 1181 469
PasI CC'CwG_GG
Cuts at: 0 1173 1521 1650
Size: 1173 348 129
Fragments arranged by size:
1173 348 129
PflMI CCAn_nnn'nTGG
Cuts at: 0 1034 1650
Size: 1034 616
PfoI T'CCnGG_A
Cuts at: 0 832 1650
Size: 832 818
PpuMI rG'GwC_Cy
Cuts at: 0 372 527 1551 1650
Size: 372 155 1024 99
Fragments arranged by size:
1024 372 155 99
PspOMI G'GGCC_C
Cuts at: 0 392 857 1650
Size: 392 465 793
Fragments arranged by size:
793 465 392
PstI C_TGCA'G
Cuts at: 0 23 113 512 752 866 941 1082 1622
Size: 23 90 399 240 114 75 141 540
Cuts at: 1622 1650
Size: 28
Fragments arranged by size:
540 399 240 141 114 90 75 28
23
PvuII CAG'CTG
Cuts at: 0 405 517 1650
Size: 405 112 1133
Fragments arranged by size:
1133 405 112
SacI G_AGCT'C
Cuts at: 0 1442 1650
Size: 1442 208
SbfI CC_TGCA'GG
Cuts at: 0 1082 1650
Size: 1082 568
SfcI C'TryA_G
Cuts at: 0 19 109 508 687 748 862 937 1078
Size: 19 90 399 179 61 114 75 141
Cuts at: 1078 1618 1650
Size: 540 32
Fragments arranged by size:
540 399 179 141 114 90 75 61
32 19
SfoI GGC'GCC
Cuts at: 0 742 1650
Size: 742 908
SmaI CCC'GGG
Cuts at: 0 205 1650
Size: 205 1445
StuI AGG'CCT
Cuts at: 0 1624 1650
Size: 1624 26
StyI C'CwwG_G
Cuts at: 0 446 953 977 1388 1650
Size: 446 507 24 411 262
Fragments arranged by size:
507 446 411 262 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 561 1650
Size: 561 1089
TatI w'GTAC_w
Cuts at: 0 419 1650
Size: 419 1231
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 364 396 434 466 536 568 814 846
Size: 364 32 38 32 70 32 246 32
Cuts at: 846 1650
Size: 804
Fragments arranged by size:
804 364 246 70 38 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 912 1117 1395 1650
Size: 912 205 278 255
Fragments arranged by size:
912 278 255 205
XmaI C'CCGG_G
Cuts at: 0 203 1650
Size: 203 1447
Enzymes that do cut:
AarI AccI AfeI AleI AlfI AlwNI ApaI AvaI
BanI BanII BbeI BbvCI BciVI BglI Bme1580I BmrI
BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrFI BsrGI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtsI CspCI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I HindIII KasI MslI MspA1I NaeI NarI
NcoI NgoMIV PasI PflMI PfoI PpuMI PspOMI PstI
PvuII SacI SbfI SfcI SfoI SmaI StuI StyI
TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AflIII AgeI AhdI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BcgI BclI BfrBI BglII BlpI BmgBI
BmtI BplI BpuEI BsaAI BsaBI BsaWI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BssHII BssSI BstAPI
BstBI BtgZI ClaI DraI DraIII EcoRI EcoRV FalI
FseI FspAI HincII HpaI KpnI MfeI MluI MmeI
MscI NdeI NheI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI ScaI SexAI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI XhoI XmnI ZraI