Restriction map for
pF1KE4450
(Linear) (Six-base) MAPSORT of: KIEE4450.seq Check: 6700 from: 1 to: 1404
KIEE4450.orf
With 182 enzymes: SgfI *
June 11, 2014 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 804 1404
Size: 804 600
AccI GT'mk_AC
Cuts at: 0 419 1404
Size: 419 985
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 716 1404
Size: 716 688
AlwNI CAG_nnn'CTG
Cuts at: 0 916 1404
Size: 916 488
ApaI G_GGCC'C
Cuts at: 0 278 1404
Size: 278 1126
BamHI G'GATC_C
Cuts at: 0 883 1404
Size: 883 521
BanI G'GyrC_C
Cuts at: 0 5 1404
Size: 5 1399
BanII G_rGCy'C
Cuts at: 0 278 851 1404
Size: 278 573 553
Fragments arranged by size:
573 553 278
BbsI GAAGACnn'nnnn_
Cuts at: 0 1185 1372 1404
Size: 1185 187 32
Fragments arranged by size:
1185 187 32
BbvCI CC'TCA_GC
Cuts at: 0 763 1141 1404
Size: 763 378 263
Fragments arranged by size:
763 378 263
BfrBI ATG'CAT
Cuts at: 0 313 1404
Size: 313 1091
BglII A'GATC_T
Cuts at: 0 381 1404
Size: 381 1023
Bme1580I G_kGCm'C
Cuts at: 0 8 278 1404
Size: 8 270 1126
Fragments arranged by size:
1126 270 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 652 676 1404
Size: 652 24 728
Fragments arranged by size:
728 652 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 1404
Size: 432 972
Bpu10I CC'TnA_GC
Cuts at: 0 763 1141 1404
Size: 763 378 263
Fragments arranged by size:
763 378 263
BseYI C'CCAG_C
Cuts at: 0 101 176 1171 1404
Size: 101 75 995 233
Fragments arranged by size:
995 233 101 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 1404
Size: 782 622
BsiEI CG_ry'CG
Cuts at: 0 291 1404
Size: 291 1113
BsiHKAI G_wGCw'C
Cuts at: 0 851 1404
Size: 851 553
Bsp1286I G_dGCh'C
Cuts at: 0 8 278 851 1404
Size: 8 270 573 553
Fragments arranged by size:
573 553 270 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 804 901 1048 1404
Size: 804 97 147 356
Fragments arranged by size:
804 356 147 97
BsrDI GCAATG_nn'
Cuts at: 0 1286 1404
Size: 1286 118
BsrFI r'CCGG_y
Cuts at: 0 1369 1404
Size: 1369 35
BstXI CCAn_nnnn'nTGG
Cuts at: 0 468 1404
Size: 468 936
BstYI r'GATC_y
Cuts at: 0 381 883 1404
Size: 381 502 521
Fragments arranged by size:
521 502 381
BstZ17I GTA'TAC
Cuts at: 0 420 1404
Size: 420 984
BtsI GCAGTG_nn'
Cuts at: 0 95 681 1404
Size: 95 586 723
Fragments arranged by size:
723 586 95
DraIII CAC_nnn'GTG
Cuts at: 0 30 1404
Size: 30 1374
EcoICRI GAG'CTC
Cuts at: 0 849 1404
Size: 849 555
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 716 1404
Size: 432 284 688
Fragments arranged by size:
688 432 284
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1107 1404
Size: 1107 297
EcoO109I rG'GnC_Cy
Cuts at: 0 274 593 631 1404
Size: 274 319 38 773
Fragments arranged by size:
773 319 274 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 528 560 585 617 1009 1041 1404
Size: 528 32 25 32 392 32 363
Fragments arranged by size:
528 392 363 32 32 32 25
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 1404
Size: 485 919
MslI CAynn'nnrTG
Cuts at: 0 868 1384 1404
Size: 868 516 20
Fragments arranged by size:
868 516 20
MspA1I CmG'CkG
Cuts at: 0 197 287 576 1404
Size: 197 90 289 828
Fragments arranged by size:
828 289 197 90
NsiI A_TGCA'T
Cuts at: 0 315 1404
Size: 315 1089
NspI r_CATG'y
Cuts at: 0 1349 1404
Size: 1349 55
PasI CC'CwG_GG
Cuts at: 0 271 1125 1404
Size: 271 854 279
Fragments arranged by size:
854 279 271
PflMI CCAn_nnn'nTGG
Cuts at: 0 1073 1404
Size: 1073 331
PpuMI rG'GwC_Cy
Cuts at: 0 593 631 1404
Size: 593 38 773
Fragments arranged by size:
773 593 38
PshAI GACnn'nnGTC
Cuts at: 0 934 1006 1404
Size: 934 72 398
Fragments arranged by size:
934 398 72
PspOMI G'GGCC_C
Cuts at: 0 274 1404
Size: 274 1130
PstI C_TGCA'G
Cuts at: 0 575 1404
Size: 575 829
PvuII CAG'CTG
Cuts at: 0 197 576 1404
Size: 197 379 828
Fragments arranged by size:
828 379 197
SacI G_AGCT'C
Cuts at: 0 851 1404
Size: 851 553
ScaI AGT'ACT
Cuts at: 0 805 1404
Size: 805 599
SfcI C'TryA_G
Cuts at: 0 192 571 1404
Size: 192 379 833
Fragments arranged by size:
833 379 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 352 1404
Size: 352 1052
TatI w'GTAC_w
Cuts at: 0 803 1404
Size: 803 601
Tth111I GACn'n_nGTC
Cuts at: 0 682 1404
Size: 682 722
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 184 1404
Size: 184 1220
Enzymes that do cut:
AarI AccI AcuI AlwNI ApaI BamHI BanI BanII
BbsI BbvCI BfrBI BglII Bme1580I BmrI BpmI Bpu10I
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BsrFI
BstXI BstYI BstZ17I BtsI DraIII EcoICRI Eco57MI EcoNI
EcoO109I Hin4I MmeI MslI MspA1I NsiI NspI PasI
PflMI PpuMI PshAI PspOMI PstI PvuII SacI ScaI
SfcI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BcgI BciVI BclI BglI
BlpI BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII
Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PciI PfoI PmeI PmlI PpiI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TstI XbaI XhoI XmaI XmnI ZraI