Restriction map for
pF1KE4294
(Linear) (Six-base) MAPSORT of: KIEE4294.seq Check: 974 from: 1 to: 6045
KIEE4294.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3881 4891 5726 6045
Size: 3881 1010 835 319
Fragments arranged by size:
3881 1010 835 319
AatII G_ACGT'C
Cuts at: 0 4196 6045
Size: 4196 1849
AccI GT'mk_AC
Cuts at: 0 803 6045
Size: 803 5242
Acc65I G'GTAC_C
Cuts at: 0 3782 6045
Size: 3782 2263
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1267 1375 1729 1826 4180 5691 6045
Size: 1267 108 354 97 2354 1511 354
Fragments arranged by size:
2354 1511 1267 354 354 108 97
AfeI AGC'GCT
Cuts at: 0 1591 3811 6045
Size: 1591 2220 2234
Fragments arranged by size:
2234 2220 1591
AflIII A'CryG_T
Cuts at: 0 5354 6045
Size: 5354 691
AhdI GACnn_n'nnGTC
Cuts at: 0 2679 4161 6045
Size: 2679 1482 1884
Fragments arranged by size:
2679 1884 1482
AleI CACnn'nnGTG
Cuts at: 0 4648 4690 5908 6045
Size: 4648 42 1218 137
Fragments arranged by size:
4648 1218 137 42
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 718 6045
Size: 718 5327
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4456 4488 6045
Size: 4456 32 1557
Fragments arranged by size:
4456 1557 32
AlwNI CAG_nnn'CTG
Cuts at: 0 270 2511 2607 2922 3651 5484 5718 6045
Size: 270 2241 96 315 729 1833 234 327
Fragments arranged by size:
2241 1833 729 327 315 270 234 96
ApaI G_GGCC'C
Cuts at: 0 1989 3519 3780 6045
Size: 1989 1530 261 2265
Fragments arranged by size:
2265 1989 1530 261
ApaLI G'TGCA_C
Cuts at: 0 5913 6045
Size: 5913 132
ApoI r'AATT_y
Cuts at: 0 1621 3826 4914 6045
Size: 1621 2205 1088 1131
Fragments arranged by size:
2205 1621 1131 1088
AvaI C'yCGr_G
Cuts at: 0 209 4113 5849 6045
Size: 209 3904 1736 196
Fragments arranged by size:
3904 1736 209 196
AvrII C'CTAG_G
Cuts at: 0 4263 6045
Size: 4263 1782
BanI G'GyrC_C
Cuts at: 0 97 205 491 2710 3020 3760 3782 3869
Size: 97 108 286 2219 310 740 22 87
Cuts at: 3869 4258 4978 5255 5418 5812 5942 5968 6045
Size: 389 720 277 163 394 130 26 77
Fragments arranged by size:
2219 740 720 394 389 310 286 277
163 130 108 97 87 77 26 22
BanII G_rGCy'C
Cuts at: 0 353 848 1727 1760 1989 2039 2818 3269
Size: 353 495 879 33 229 50 779 451
Cuts at: 3269 3519 3780 4007 5186 6045
Size: 250 261 227 1179 859
Fragments arranged by size:
1179 879 859 779 495 451 353 261
250 229 227 50 33
BbeI G_GCGC'C
Cuts at: 0 209 4262 5816 5972 6045
Size: 209 4053 1554 156 73
Fragments arranged by size:
4053 1554 209 156 73
BbsI GAAGACnn'nnnn_
Cuts at: 0 626 1814 2861 3578 5057 6045
Size: 626 1188 1047 717 1479 988
Fragments arranged by size:
1479 1188 1047 988 717 626
BbvCI CC'TCA_GC
Cuts at: 0 3865 6045
Size: 3865 2180
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 838 872 2478 2512 6045
Size: 838 34 1606 34 3533
Fragments arranged by size:
3533 1606 838 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 4857 6045
Size: 4857 1188
BclI T'GATC_A
Cuts at: 0 1922 2843 3107 3423 6045
Size: 1922 921 264 316 2622
Fragments arranged by size:
2622 1922 921 316 264
BglI GCCn_nnn'nGGC
Cuts at: 0 376 2116 2563 3752 5893 6045
Size: 376 1740 447 1189 2141 152
Fragments arranged by size:
2141 1740 1189 447 376 152
BglII A'GATC_T
Cuts at: 0 1967 5165 6045
Size: 1967 3198 880
Fragments arranged by size:
3198 1967 880
BlpI GC'TnA_GC
Cuts at: 0 1883 4015 6045
Size: 1883 2132 2030
Fragments arranged by size:
2132 2030 1883
Bme1580I G_kGCm'C
Cuts at: 0 494 1989 3491 3519 3780 4279 4837 4981
Size: 494 1495 1502 28 261 499 558 144
Cuts at: 4981 5319 5476 5917 6045
Size: 338 157 441 128
Fragments arranged by size:
1502 1495 558 499 494 441 338 261
157 144 128 28
BmgBI CAC'GTC
Cuts at: 0 2463 2484 2598 3315 3408 5445 6045
Size: 2463 21 114 717 93 2037 600
Fragments arranged by size:
2463 2037 717 600 114 93 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 3527 4402 5068 6045
Size: 3527 875 666 977
Fragments arranged by size:
3527 977 875 666
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5448 6045
Size: 5448 597
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 319 1218 1497 1984 2023 2191 2346
Size: 241 78 899 279 487 39 168 155
Cuts at: 2346 2644 3718 3733 4021 4393 4747 4853 5182
Size: 298 1074 15 288 372 354 106 329
Cuts at: 5182 5200 5818 5976 6040 6045
Size: 18 618 158 64 5
Fragments arranged by size:
1074 899 618 487 372 354 329 298
288 279 241 168 158 155 106 78
64 39 18 15 5
Bpu10I CC'TnA_GC
Cuts at: 0 1766 2542 3865 6045
Size: 1766 776 1323 2180
Fragments arranged by size:
2180 1766 1323 776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 1452 1454 2040 4807 5553 6045
Size: 439 1013 2 586 2767 746 492
Fragments arranged by size:
2767 1013 746 586 492 439 2
BsaI GGTCTCn'nnnn_
Cuts at: 0 188 5695 5741 6045
Size: 188 5507 46 304
Fragments arranged by size:
5507 304 188 46
BsaAI yAC'GTr
Cuts at: 0 2160 2468 3486 6045
Size: 2160 308 1018 2559
Fragments arranged by size:
2559 2160 1018 308
BsaBI GATnn'nnATC
Cuts at: 0 1924 3106 5485 6045
Size: 1924 1182 2379 560
Fragments arranged by size:
2379 1924 1182 560
BsaHI Gr'CG_yC
Cuts at: 0 206 2644 3466 4193 4259 5813 5969 6045
Size: 206 2438 822 727 66 1554 156 76
Fragments arranged by size:
2438 1554 822 727 206 156 76 66
BsaWI w'CCGG_w
Cuts at: 0 911 6045
Size: 911 5134
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1674 1704 3592 3622 4981 5011 5140 5170
Size: 1674 30 1888 30 1359 30 129 30
Cuts at: 5170 5500 5530 6045
Size: 330 30 515
Fragments arranged by size:
1888 1674 1359 515 330 129 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 238 2203 4684 5257 5479 5523 6019
Size: 67 171 1965 2481 573 222 44 496
Cuts at: 6019 6045
Size: 26
Fragments arranged by size:
2481 1965 573 496 222 171 67 44
26
BseYI C'CCAG_C
Cuts at: 0 25 248 366 446 691 739 1770 1779
Size: 25 223 118 80 245 48 1031 9
Cuts at: 1779 2181 3518 4505 5338 5469 5578 5586 5816
Size: 402 1337 987 833 131 109 8 230
Cuts at: 5816 5963 6009 6045
Size: 147 46 36
Fragments arranged by size:
1337 1031 987 833 402 245 230 223
147 131 118 109 80 48 46 36
25 9 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 979 2490 2620 3574 3916 4114 4373
Size: 427 552 1511 130 954 342 198 259
Cuts at: 4373 4489 4741 5241 6045
Size: 116 252 500 804
Fragments arranged by size:
1511 954 804 552 500 427 342 259
252 198 130 116
BsiEI CG_ry'CG
Cuts at: 0 4531 6045
Size: 4531 1514
BsiHKAI G_wGCw'C
Cuts at: 0 848 1727 1760 1884 2039 3719 4007 4205
Size: 848 879 33 124 155 1680 288 198
Cuts at: 4205 4361 5011 5917 6045
Size: 156 650 906 128
Fragments arranged by size:
1680 906 879 848 650 288 198 156
155 128 124 33
BsmBI CGTCTCn'nnnn_
Cuts at: 0 79 6045
Size: 79 5966
Bsp1286I G_dGCh'C
Cuts at: 0 353 494 848 1727 1760 1884 1989 2039
Size: 353 141 354 879 33 124 105 50
Cuts at: 2039 2818 3269 3491 3519 3719 3780 4007 4205
Size: 779 451 222 28 200 61 227 198
Cuts at: 4205 4279 4361 4837 4981 5011 5186 5319 5476
Size: 74 82 476 144 30 175 133 157
Cuts at: 5476 5917 6045
Size: 441 128
Fragments arranged by size:
879 779 476 451 441 354 353 227
222 200 198 175 157 144 141 133
128 124 105 82 74 61 50 33
30 28
BspEI T'CCGG_A
Cuts at: 0 911 6045
Size: 911 5134
BspHI T'CATG_A
Cuts at: 0 3668 4493 6045
Size: 3668 825 1552
Fragments arranged by size:
3668 1552 825
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 224 1492 1883 2684 3137 3881 4136 4891
Size: 224 1268 391 801 453 744 255 755
Cuts at: 4891 5726 6045
Size: 835 319
Fragments arranged by size:
1268 835 801 755 744 453 391 319
255 224
BsrBI CCG'CTC
Cuts at: 0 2348 3597 3987 4014 6045
Size: 2348 1249 390 27 2031
Fragments arranged by size:
2348 2031 1249 390 27
BsrDI GCAATG_nn'
Cuts at: 0 623 1830 1868 5130 5831 6045
Size: 623 1207 38 3262 701 214
Fragments arranged by size:
3262 1207 701 623 214 38
BsrFI r'CCGG_y
Cuts at: 0 2046 2106 2707 3849 6014 6045
Size: 2046 60 601 1142 2165 31
Fragments arranged by size:
2165 2046 1142 601 60 31
BsrGI T'GTAC_A
Cuts at: 0 5456 6045
Size: 5456 589
BstAPI GCAn_nnn'nTGC
Cuts at: 0 414 3486 3939 5871 6045
Size: 414 3072 453 1932 174
Fragments arranged by size:
3072 1932 453 414 174
BstBI TT'CG_AA
Cuts at: 0 1103 6045
Size: 1103 4942
BstEII G'GTnAC_C
Cuts at: 0 136 6045
Size: 136 5909
BstXI CCAn_nnnn'nTGG
Cuts at: 0 867 1265 1962 5091 5280 6045
Size: 867 398 697 3129 189 765
Fragments arranged by size:
3129 867 765 697 398 189
BstYI r'GATC_y
Cuts at: 0 294 1260 1506 1967 2798 4774 5165 5480
Size: 294 966 246 461 831 1976 391 315
Cuts at: 5480 5732 6045
Size: 252 313
Fragments arranged by size:
1976 966 831 461 391 315 313 294
252 246
Bsu36I CC'TnA_GG
Cuts at: 0 2746 2860 5347 5423 6045
Size: 2746 114 2487 76 622
Fragments arranged by size:
2746 2487 622 114 76
BtgI C'CryG_G
Cuts at: 0 80 198 353 371 2075 2195 2249 2739
Size: 80 118 155 18 1704 120 54 490
Cuts at: 2739 3009 3386 4646 4967 5084 5201 5690 5888
Size: 270 377 1260 321 117 117 489 198
Cuts at: 5888 6045
Size: 157
Fragments arranged by size:
1704 1260 490 489 377 321 270 198
157 155 120 118 117 117 80 54
18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 5025 5421 6045
Size: 5025 396 624
Fragments arranged by size:
5025 624 396
BtsI GCAGTG_nn'
Cuts at: 0 3762 6045
Size: 3762 2283
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1459 1494 2231 2266 6045
Size: 1459 35 737 35 3779
Fragments arranged by size:
3779 1459 737 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 2030 5939 6045
Size: 2030 3909 106
Fragments arranged by size:
3909 2030 106
EaeI y'GGCC_r
Cuts at: 0 599 2108 2117 2198 2828 2924 3587 3683
Size: 599 1509 9 81 630 96 663 96
Cuts at: 3683 3800 4892 5405 5948 6045
Size: 117 1092 513 543 97
Fragments arranged by size:
1509 1092 663 630 599 543 513 117
97 96 96 81 9
EarI CTCTTCn'nnn_
Cuts at: 0 1705 2897 3697 6045
Size: 1705 1192 800 2348
Fragments arranged by size:
2348 1705 1192 800
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3133 4429 6045
Size: 3133 1296 1616
Fragments arranged by size:
3133 1616 1296
EcoICRI GAG'CTC
Cuts at: 0 846 1725 1758 2037 4005 6045
Size: 846 879 33 279 1968 2040
Fragments arranged by size:
2040 1968 879 846 279 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 319 1218 1267 1375 1497 1729 1826
Size: 241 78 899 49 108 122 232 97
Cuts at: 1826 1984 2023 2191 2346 2644 3718 3733 4021
Size: 158 39 168 155 298 1074 15 288
Cuts at: 4021 4180 4393 4747 4853 5182 5200 5691 5818
Size: 159 213 354 106 329 18 491 127
Cuts at: 5818 5976 6040 6045
Size: 158 64 5
Fragments arranged by size:
1074 899 491 354 329 298 288 241
232 213 168 159 158 158 155 127
122 108 106 97 78 64 49 39
18 15 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 220 2551 3745 4714 5664 5800 6045
Size: 220 2331 1194 969 950 136 245
Fragments arranged by size:
2331 1194 969 950 245 220 136
EcoO109I rG'GnC_Cy
Cuts at: 0 213 433 650 897 1852 1933 1985 1986
Size: 213 220 217 247 955 81 52 1
Cuts at: 1986 2151 2238 2673 4293 4563 4707 5334 5561
Size: 165 87 435 1620 270 144 627 227
Cuts at: 5561 5760 6045
Size: 199 285
Fragments arranged by size:
1620 955 627 435 285 270 247 227
220 217 213 199 165 144 87 81
52 1
EcoRI G'AATT_C
Cuts at: 0 4914 6045
Size: 4914 1131
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1661 3078 6045
Size: 1661 1417 2967
Fragments arranged by size:
2967 1661 1417
HaeII r_GCGC'y
Cuts at: 0 209 1593 2182 2580 3813 3964 4262 4386
Size: 209 1384 589 398 1233 151 298 124
Cuts at: 4386 4492 5816 5972 6045
Size: 106 1324 156 73
Fragments arranged by size:
1384 1324 1233 589 398 298 209 156
151 124 106 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2665 2697 3592 3624 4067 4099 4147 4179
Size: 2665 32 895 32 443 32 48 32
Cuts at: 4179 4293 4325 4327 4357 4359 4389 4981 5013
Size: 114 32 2 30 2 30 592 32
Cuts at: 5013 5140 5172 5500 5532 5768 5800 6045
Size: 127 32 328 32 236 32 245
Fragments arranged by size:
2665 895 592 443 328 245 236 127
114 48 32 32 32 32 32 32
32 32 32 30 30 2 2
HincII GTy'rAC
Cuts at: 0 2127 5097 6045
Size: 2127 2970 948
Fragments arranged by size:
2970 2127 948
HindIII A'AGCT_T
Cuts at: 0 1292 6045
Size: 1292 4753
KasI G'GCGC_C
Cuts at: 0 205 4258 5812 5968 6045
Size: 205 4053 1554 156 77
Fragments arranged by size:
4053 1554 205 156 77
KpnI G_GTAC'C
Cuts at: 0 3786 6045
Size: 3786 2259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 953 3335 4145 5687 6045
Size: 953 2382 810 1542 358
Fragments arranged by size:
2382 1542 953 810 358
MscI TGG'CCA
Cuts at: 0 2119 2200 2830 2926 6045
Size: 2119 81 630 96 3119
Fragments arranged by size:
3119 2119 630 96 81
MslI CAynn'nnrTG
Cuts at: 0 2402 4177 4582 4648 4690 4717 5089 5908
Size: 2402 1775 405 66 42 27 372 819
Cuts at: 5908 6045
Size: 137
Fragments arranged by size:
2402 1775 819 405 372 137 66 42
27
MspA1I CmG'CkG
Cuts at: 0 61 200 252 450 1452 1779 1797 1887
Size: 61 139 52 198 1002 327 18 90
Cuts at: 1887 2451 2622 2631 3642 4533 4873 4994 5102
Size: 564 171 9 1011 891 340 121 108
Cuts at: 5102 5310 5820 6045
Size: 208 510 225
Fragments arranged by size:
1011 1002 891 564 510 340 327 225
208 198 171 139 121 108 90 61
52 18 9
NaeI GCC'GGC
Cuts at: 0 2048 6016 6045
Size: 2048 3968 29
Fragments arranged by size:
3968 2048 29
NarI GG'CG_CC
Cuts at: 0 206 4259 5813 5969 6045
Size: 206 4053 1554 156 76
Fragments arranged by size:
4053 1554 206 156 76
NcoI C'CATG_G
Cuts at: 0 80 3386 5084 5201 5690 6045
Size: 80 3306 1698 117 489 355
Fragments arranged by size:
3306 1698 489 355 117 80
NgoMIV G'CCGG_C
Cuts at: 0 2046 6014 6045
Size: 2046 3968 31
Fragments arranged by size:
3968 2046 31
NspI r_CATG'y
Cuts at: 0 12 33 3930 4083 4833 5358 5640 6045
Size: 12 21 3897 153 750 525 282 405
Fragments arranged by size:
3897 750 525 405 282 153 21 12
PasI CC'CwG_GG
Cuts at: 0 797 893 1265 1398 4071 4629 4738 6045
Size: 797 96 372 133 2673 558 109 1307
Fragments arranged by size:
2673 1307 797 558 372 133 109 96
PciI A'CATG_T
Cuts at: 0 5354 6045
Size: 5354 691
PflMI CCAn_nnn'nTGG
Cuts at: 0 1124 2030 2511 2922 3254 3798 6045
Size: 1124 906 481 411 332 544 2247
Fragments arranged by size:
2247 1124 906 544 481 411 332
PfoI T'CCnGG_A
Cuts at: 0 750 1035 1594 1751 1961 2726 3695 4675
Size: 750 285 559 157 210 765 969 980
Cuts at: 4675 6045
Size: 1370
Fragments arranged by size:
1370 980 969 765 750 559 285 210
157
PmlI CAC'GTG
Cuts at: 0 2468 6045
Size: 2468 3577
PpuMI rG'GwC_Cy
Cuts at: 0 213 433 1933 2673 4563 5334 5561 5760
Size: 213 220 1500 740 1890 771 227 199
Cuts at: 5760 6045
Size: 285
Fragments arranged by size:
1890 1500 771 740 285 227 220 213
199
PspOMI G'GGCC_C
Cuts at: 0 1985 3515 3776 6045
Size: 1985 1530 261 2269
Fragments arranged by size:
2269 1985 1530 261
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2902 2934 6045
Size: 2902 32 3111
Fragments arranged by size:
3111 2902 32
PstI C_TGCA'G
Cuts at: 0 147 442 1489 1836 2552 2681 2795 3134
Size: 147 295 1047 347 716 129 114 339
Cuts at: 3134 3647 3944 4487 5173 5723 6045
Size: 513 297 543 686 550 322
Fragments arranged by size:
1047 716 686 550 543 513 347 339
322 297 295 147 129 114
PvuII CAG'CTG
Cuts at: 0 61 252 1452 1779 1797 1887 2451 2622
Size: 61 191 1200 327 18 90 564 171
Cuts at: 2622 2631 4873 4994 5310 6045
Size: 9 2242 121 316 735
Fragments arranged by size:
2242 1200 735 564 327 316 191 171
121 90 61 18 9
SacI G_AGCT'C
Cuts at: 0 848 1727 1760 2039 4007 6045
Size: 848 879 33 279 1968 2038
Fragments arranged by size:
2038 1968 879 848 279 33
SacII CC_GC'GG
Cuts at: 0 201 6045
Size: 201 5844
SanDI GG'GwC_CC
Cuts at: 0 1933 5334 5760 6045
Size: 1933 3401 426 285
Fragments arranged by size:
3401 1933 426 285
SapI GCTCTTCn'nnn_
Cuts at: 0 1705 2897 3697 6045
Size: 1705 1192 800 2348
Fragments arranged by size:
2348 1705 1192 800
SbfI CC_TGCA'GG
Cuts at: 0 1489 2552 5723 6045
Size: 1489 1063 3171 322
Fragments arranged by size:
3171 1489 1063 322
ScaI AGT'ACT
Cuts at: 0 715 2911 3559 6045
Size: 715 2196 648 2486
Fragments arranged by size:
2486 2196 715 648
SexAI A'CCwGG_T
Cuts at: 0 429 4922 6045
Size: 429 4493 1123
Fragments arranged by size:
4493 1123 429
SfcI C'TryA_G
Cuts at: 0 143 438 1144 1485 1832 2548 2677 2791
Size: 143 295 706 341 347 716 129 114
Cuts at: 2791 3130 3216 3643 3940 4483 5169 5719 6045
Size: 339 86 427 297 543 686 550 326
Fragments arranged by size:
716 706 686 550 543 427 347 341
339 326 297 295 143 129 114 86
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2116 6045
Size: 2116 3929
SfoI GGC'GCC
Cuts at: 0 207 4260 5814 5970 6045
Size: 207 4053 1554 156 75
Fragments arranged by size:
4053 1554 207 156 75
SmaI CCC'GGG
Cuts at: 0 211 6045
Size: 211 5834
SmlI C'TyrA_G
Cuts at: 0 418 1431 1469 2055 4822 5568 6045
Size: 418 1013 38 586 2767 746 477
Fragments arranged by size:
2767 1013 746 586 477 418 38
SphI G_CATG'C
Cuts at: 0 3930 6045
Size: 3930 2115
SspI AAT'ATT
Cuts at: 0 632 2808 6045
Size: 632 2176 3237
Fragments arranged by size:
3237 2176 632
StuI AGG'CCT
Cuts at: 0 229 4308 4858 6045
Size: 229 4079 550 1187
Fragments arranged by size:
4079 1187 550 229
StyI C'CwwG_G
Cuts at: 0 80 473 2063 2234 3386 4263 4430 4859
Size: 80 393 1590 171 1152 877 167 429
Cuts at: 4859 5084 5201 5279 5372 5690 6045
Size: 225 117 78 93 318 355
Fragments arranged by size:
1590 1152 877 429 393 355 318 225
171 167 117 93 80 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2810 3022 3688 4102 6045
Size: 2810 212 666 414 1943
Fragments arranged by size:
2810 1943 666 414 212
TatI w'GTAC_w
Cuts at: 0 32 713 2909 3557 4640 5456 6045
Size: 32 681 2196 648 1083 816 589
Fragments arranged by size:
2196 1083 816 681 648 589 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1674 1706 3397 3429 6045
Size: 1674 32 1691 32 2616
Fragments arranged by size:
2616 1691 1674 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1684 3406 3577 6045
Size: 1684 1722 171 2468
Fragments arranged by size:
2468 1722 1684 171
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1033 1305 1999 2047 2298 3693 3754 6045
Size: 1033 272 694 48 251 1395 61 2291
Fragments arranged by size:
2291 1395 1033 694 272 251 61 48
XmaI C'CCGG_G
Cuts at: 0 209 6045
Size: 209 5836
XmnI GAAnn'nnTTC
Cuts at: 0 565 3898 6045
Size: 565 3333 2147
Fragments arranged by size:
3333 2147 565
ZraI GAC'GTC
Cuts at: 0 4194 6045
Size: 4194 1851
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI
AvrII BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmlI PpuMI
PspOMI PsrI PstI PvuII SacI SacII SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SmaI SmlI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AflII AgeI AscI AseI AsiSI BaeI BamHI
BfrBI BmtI BsiWI BsmI BssHII BssSI BstZ17I ClaI
DraI DrdI EagI EcoRV FseI FspI FspAI HpaI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PmeI PpiI PshAI PsiI PspXI PvuI RsrII SalI
SgfI SgrAI SnaBI SpeI SrfI SwaI XbaI XhoI