Restriction map for
pF1KE4224
(Linear) (Six-base) MAPSORT of: KIEE4224.seq Check: 2908 from: 1 to: 2358
KIEE4224.orf
With 182 enzymes: SgfI *
August 18, 2011 15:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1765 2358
Size: 1765 593
AccI GT'mk_AC
Cuts at: 0 1838 2358
Size: 1838 520
AhdI GACnn_n'nnGTC
Cuts at: 0 152 2358
Size: 152 2206
AlwNI CAG_nnn'CTG
Cuts at: 0 15 29 1581 2358
Size: 15 14 1552 777
Fragments arranged by size:
1552 777 15 14
ApoI r'AATT_y
Cuts at: 0 265 273 352 447 1335 2358
Size: 265 8 79 95 888 1023
Fragments arranged by size:
1023 888 265 95 79 8
AvaI C'yCGr_G
Cuts at: 0 85 535 2119 2198 2358
Size: 85 450 1584 79 160
Fragments arranged by size:
1584 450 160 85 79
BamHI G'GATC_C
Cuts at: 0 62 2358
Size: 62 2296
BanII G_rGCy'C
Cuts at: 0 116 746 1754 2358
Size: 116 630 1008 604
Fragments arranged by size:
1008 630 604 116
BbsI GAAGACnn'nnnn_
Cuts at: 0 1363 1387 2358
Size: 1363 24 971
Fragments arranged by size:
1363 971 24
BciVI GTATCCnnnnn_n'
Cuts at: 0 2219 2358
Size: 2219 139
BfrBI ATG'CAT
Cuts at: 0 245 1205 2358
Size: 245 960 1153
Fragments arranged by size:
1153 960 245
BglI GCCn_nnn'nGGC
Cuts at: 0 1791 2358
Size: 1791 567
BglII A'GATC_T
Cuts at: 0 922 2358
Size: 922 1436
BmrI ACTGGGnnnn_n'
Cuts at: 0 2022 2358
Size: 2022 336
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 310 947 1351 1873 2265 2358
Size: 37 273 637 404 522 392 93
Fragments arranged by size:
637 522 404 392 273 93 37
Bpu10I CC'TnA_GC
Cuts at: 0 1748 2358
Size: 1748 610
BsaAI yAC'GTr
Cuts at: 0 262 2358
Size: 262 2096
BsaHI Gr'CG_yC
Cuts at: 0 1726 2358
Size: 1726 632
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1404 2135 2358
Size: 1404 731 223
Fragments arranged by size:
1404 731 223
BseYI C'CCAG_C
Cuts at: 0 167 2358
Size: 167 2191
BsiEI CG_ry'CG
Cuts at: 0 2044 2358
Size: 2044 314
BsiHKAI G_wGCw'C
Cuts at: 0 116 746 920 2358
Size: 116 630 174 1438
Fragments arranged by size:
1438 630 174 116
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1937 2358
Size: 1937 421
Bsp1286I G_dGCh'C
Cuts at: 0 116 746 920 1754 2358
Size: 116 630 174 834 604
Fragments arranged by size:
834 630 604 174 116
BspHI T'CATG_A
Cuts at: 0 746 1409 2358
Size: 746 663 949
Fragments arranged by size:
949 746 663
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1765 2358
Size: 1765 593
BsrDI GCAATG_nn'
Cuts at: 0 1323 2358
Size: 1323 1035
BsrFI r'CCGG_y
Cuts at: 0 509 2358
Size: 509 1849
BsrGI T'GTAC_A
Cuts at: 0 1247 2271 2358
Size: 1247 1024 87
Fragments arranged by size:
1247 1024 87
BssSI C'ACGA_G
Cuts at: 0 1223 1469 2358
Size: 1223 246 889
Fragments arranged by size:
1223 889 246
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1911 2358
Size: 1911 447
BstYI r'GATC_y
Cuts at: 0 62 922 2358
Size: 62 860 1436
Fragments arranged by size:
1436 860 62
BtgI C'CryG_G
Cuts at: 0 46 1829 2358
Size: 46 1783 529
Fragments arranged by size:
1783 529 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 123 2358
Size: 123 2235
BtsI GCAGTG_nn'
Cuts at: 0 1090 2358
Size: 1090 1268
ClaI AT'CG_AT
Cuts at: 0 711 2358
Size: 711 1647
DrdI GACnn_nn'nnGTC
Cuts at: 0 2043 2358
Size: 2043 315
EaeI y'GGCC_r
Cuts at: 0 1832 2358
Size: 1832 526
EarI CTCTTCn'nnn_
Cuts at: 0 734 1064 1795 2358
Size: 734 330 731 563
Fragments arranged by size:
734 731 563 330
EcoICRI GAG'CTC
Cuts at: 0 114 744 2358
Size: 114 630 1614
Fragments arranged by size:
1614 630 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 310 947 1351 1873 2265 2358
Size: 37 273 637 404 522 392 93
Fragments arranged by size:
637 522 404 392 273 93 37
EcoRI G'AATT_C
Cuts at: 0 1335 2358
Size: 1335 1023
EcoRV GAT'ATC
Cuts at: 0 492 2358
Size: 492 1866
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 385 2358
Size: 385 1973
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 54 86 138 170 907 939 2002 2034
Size: 54 32 52 32 737 32 1063 32
Cuts at: 2034 2358
Size: 324
Fragments arranged by size:
1063 737 324 54 52 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1070 1151 1508 2101 2117 2170 2358
Size: 1070 81 357 593 16 53 188
Fragments arranged by size:
1070 593 357 188 81 53 16
MslI CAynn'nnrTG
Cuts at: 0 521 2358
Size: 521 1837
MspA1I CmG'CkG
Cuts at: 0 171 1948 2101 2358
Size: 171 1777 153 257
Fragments arranged by size:
1777 257 171 153
NsiI A_TGCA'T
Cuts at: 0 247 1207 2358
Size: 247 960 1151
Fragments arranged by size:
1151 960 247
NspI r_CATG'y
Cuts at: 0 876 1316 2358
Size: 876 440 1042
Fragments arranged by size:
1042 876 440
PasI CC'CwG_GG
Cuts at: 0 1954 2358
Size: 1954 404
PflMI CCAn_nnn'nTGG
Cuts at: 0 1491 1986 2358
Size: 1491 495 372
Fragments arranged by size:
1491 495 372
PfoI T'CCnGG_A
Cuts at: 0 53 821 1962 2358
Size: 53 768 1141 396
Fragments arranged by size:
1141 768 396 53
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 518 550 2358
Size: 518 32 1808
Fragments arranged by size:
1808 518 32
PspXI vC'TCGA_Gb
Cuts at: 0 2198 2358
Size: 2198 160
PstI C_TGCA'G
Cuts at: 0 11 557 1344 2358
Size: 11 546 787 1014
Fragments arranged by size:
1014 787 546 11
PvuI CG_AT'CG
Cuts at: 0 2044 2358
Size: 2044 314
PvuII CAG'CTG
Cuts at: 0 1948 2101 2358
Size: 1948 153 257
Fragments arranged by size:
1948 257 153
SacI G_AGCT'C
Cuts at: 0 116 746 2358
Size: 116 630 1612
Fragments arranged by size:
1612 630 116
SapI GCTCTTCn'nnn_
Cuts at: 0 734 2358
Size: 734 1624
SfcI C'TryA_G
Cuts at: 0 7 553 682 1340 1943 2156 2358
Size: 7 546 129 658 603 213 202
Fragments arranged by size:
658 603 546 213 202 129 7
SmaI CCC'GGG
Cuts at: 0 2121 2358
Size: 2121 237
SmlI C'TyrA_G
Cuts at: 0 85 535 2198 2358
Size: 85 450 1663 160
Fragments arranged by size:
1663 450 160 85
SspI AAT'ATT
Cuts at: 0 1680 2358
Size: 1680 678
StuI AGG'CCT
Cuts at: 0 834 2358
Size: 834 1524
StyI C'CwwG_G
Cuts at: 0 344 770 1239 1789 1886 2358
Size: 344 426 469 550 97 472
Fragments arranged by size:
550 472 469 426 344 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1090 2358
Size: 1090 1268
TatI w'GTAC_w
Cuts at: 0 1247 2271 2358
Size: 1247 1024 87
Fragments arranged by size:
1247 1024 87
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1827 2358
Size: 1827 531
XhoI C'TCGA_G
Cuts at: 0 85 535 2198 2358
Size: 85 450 1663 160
Fragments arranged by size:
1663 450 160 85
XmaI C'CCGG_G
Cuts at: 0 2119 2358
Size: 2119 239
Enzymes that do cut:
AarI AccI AhdI AlwNI ApoI AvaI BamHI BanII
BbsI BciVI BfrBI BglI BglII BmrI BpmI Bpu10I
BsaAI BsaHI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrDI BsrFI BsrGI BssSI BstXI BstYI
BtgI BtgZI BtsI ClaI DrdI EaeI EarI EcoICRI
Eco57MI EcoRI EcoRV FalI Hin4I MmeI MslI MspA1I
NsiI NspI PasI PflMI PfoI PpiI PspXI PstI
PvuI PvuII SacI SapI SfcI SmaI SmlI SspI
StuI StyI TaqII TatI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BanI BbeI BbvCI BcgI BclI BlpI
Bme1580I BmgBI BmtI BplI BpuEI BsaI BsaBI BsaWI
BsaXI BsgI BsiWI BsmI BspEI BsrBI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I CspCI DraI DraIII EagI
EciI EcoNI EcoO109I FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PciI PmeI PmlI PpuMI PshAI PsiI PspOMI PsrI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI
TstI Tth111I XbaI XmnI ZraI