Restriction map for
pF1KE3718
(Linear) (Six-base) MAPSORT of: KIEE3718.seq Check: 1469 from: 1 to: 7413
KIEE3718.orf
With 182 enzymes: SgfI *
June 11, 2014 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 598 1063 1213 1783 5686 6988 7413
Size: 598 465 150 570 3903 1302 425
Fragments arranged by size:
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AccI GT'mk_AC
Cuts at: 0 3843 5588 7413
Size: 3843 1745 1825
Fragments arranged by size:
3843 1825 1745
Acc65I G'GTAC_C
Cuts at: 0 937 3133 4633 4708 7413
Size: 937 2196 1500 75 2705
Fragments arranged by size:
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AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1354 2691 5156 5333 6301 6887 7413
Size: 1354 1337 2465 177 968 586 526
Fragments arranged by size:
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AflIII A'CryG_T
Cuts at: 0 1435 2086 2684 3001 3115 6473 7373 7413
Size: 1435 651 598 317 114 3358 900 40
Fragments arranged by size:
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AhdI GACnn_n'nnGTC
Cuts at: 0 4122 7272 7413
Size: 4122 3150 141
Fragments arranged by size:
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AleI CACnn'nnGTG
Cuts at: 0 1690 3368 7413
Size: 1690 1678 4045
Fragments arranged by size:
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AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 935 4711 7190 7413
Size: 935 3776 2479 223
Fragments arranged by size:
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AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4658 4690 7413
Size: 4658 32 2723
Fragments arranged by size:
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AlwNI CAG_nnn'CTG
Cuts at: 0 1554 3186 5784 5956 7135 7413
Size: 1554 1632 2598 172 1179 278
Fragments arranged by size:
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ApaI G_GGCC'C
Cuts at: 0 5291 6260 7003 7413
Size: 5291 969 743 410
Fragments arranged by size:
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ApaLI G'TGCA_C
Cuts at: 0 390 674 2970 4072 7413
Size: 390 284 2296 1102 3341
Fragments arranged by size:
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ApoI r'AATT_y
Cuts at: 0 2556 7413
Size: 2556 4857
AseI AT'TA_AT
Cuts at: 0 1931 1988 7413
Size: 1931 57 5425
Fragments arranged by size:
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AvaI C'yCGr_G
Cuts at: 0 1789 2125 3650 3689 4002 4112 4218 4301
Size: 1789 336 1525 39 313 110 106 83
Cuts at: 4301 6053 6137 6149 6890 7413
Size: 1752 84 12 741 523
Fragments arranged by size:
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106 84 83 39 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1944 1977 2325 2358 2613 2646 3218 3251
Size: 1944 33 348 33 255 33 572 33
Cuts at: 3251 4625 4658 7413
Size: 1374 33 2755
Fragments arranged by size:
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33 33 33
BamHI G'GATC_C
Cuts at: 0 3055 7413
Size: 3055 4358
BanI G'GyrC_C
Cuts at: 0 586 700 937 1312 1801 3133 3283 3598
Size: 586 114 237 375 489 1332 150 315
Cuts at: 3598 4022 4245 4633 4708 5596 6217 6829 7413
Size: 424 223 388 75 888 621 612 584
Fragments arranged by size:
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388 375 315 237 223 150 114 75
BanII G_rGCy'C
Cuts at: 0 2983 5291 6260 6899 6963 7003 7413
Size: 2983 2308 969 639 64 40 410
Fragments arranged by size:
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BbeI G_GCGC'C
Cuts at: 0 1316 1805 7413
Size: 1316 489 5608
Fragments arranged by size:
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BbsI GAAGACnn'nnnn_
Cuts at: 0 5766 6124 7413
Size: 5766 358 1289
Fragments arranged by size:
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BbvCI CC'TCA_GC
Cuts at: 0 4172 6274 6824 7413
Size: 4172 2102 550 589
Fragments arranged by size:
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BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3439 3473 4440 4474 7413
Size: 3439 34 967 34 2939
Fragments arranged by size:
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BciVI GTATCCnnnnn_n'
Cuts at: 0 139 372 1972 2326 3502 7413
Size: 139 233 1600 354 1176 3911
Fragments arranged by size:
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BclI T'GATC_A
Cuts at: 0 5888 7413
Size: 5888 1525
BfrBI ATG'CAT
Cuts at: 0 73 1132 1136 7413
Size: 73 1059 4 6277
Fragments arranged by size:
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BglI GCCn_nnn'nGGC
Cuts at: 0 350 515 632 1214 1904 3158 3416 3644
Size: 350 165 117 582 690 1254 258 228
Cuts at: 3644 5480 5860 6215 6364 7413
Size: 1836 380 355 149 1049
Fragments arranged by size:
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BglII A'GATC_T
Cuts at: 0 6265 7413
Size: 6265 1148
BlpI GC'TnA_GC
Cuts at: 0 370 7193 7413
Size: 370 6823 220
Fragments arranged by size:
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Bme1580I G_kGCm'C
Cuts at: 0 394 628 671 678 1093 1164 2974 3040
Size: 394 234 43 7 415 71 1810 66
Cuts at: 3040 3302 3412 3690 4076 4209 4704 5291 6260
Size: 262 110 278 386 133 495 587 969
Cuts at: 6260 6832 7003 7413
Size: 572 171 410
Fragments arranged by size:
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66 43 7
BmgBI CAC'GTC
Cuts at: 0 1910 3297 7413
Size: 1910 1387 4116
Fragments arranged by size:
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BmrI ACTGGGnnnn_n'
Cuts at: 0 1583 2834 3176 3249 3434 4100 4714 5280
Size: 1583 1251 342 73 185 666 614 566
Cuts at: 5280 5955 6311 6568 6736 6780 7040 7199 7413
Size: 675 356 257 168 44 260 159 214
Fragments arranged by size:
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BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2076 2649 3822 7413
Size: 2076 573 1173 3591
Fragments arranged by size:
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BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1008 1028 2225 3312 4660 5000 5590 6197
Size: 1008 20 1197 1087 1348 340 590 607
Cuts at: 6197 6680 6803 6971 7207 7364 7413
Size: 483 123 168 236 157 49
Fragments arranged by size:
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Bpu10I CC'TnA_GC
Cuts at: 0 2173 4172 6274 6824 7413
Size: 2173 1999 2102 550 589
Fragments arranged by size:
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BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6741 7413
Size: 6741 672
BsaI GGTCTCn'nnnn_
Cuts at: 0 188 1114 2215 2662 3212 6775 7413
Size: 188 926 1101 447 550 3563 638
Fragments arranged by size:
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BsaAI yAC'GTr
Cuts at: 0 266 6476 7413
Size: 266 6210 937
Fragments arranged by size:
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BsaBI GATnn'nnATC
Cuts at: 0 1366 7413
Size: 1366 6047
BsaHI Gr'CG_yC
Cuts at: 0 1313 1802 4310 7413
Size: 1313 489 2508 3103
Fragments arranged by size:
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BsaWI w'CCGG_w
Cuts at: 0 5621 7413
Size: 5621 1792
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 481 511 753 783 3420 3450 4384 4414
Size: 481 30 242 30 2637 30 934 30
Cuts at: 4414 4658 4688 6483 6513 6969 6999 7413
Size: 244 30 1795 30 456 30 414
Fragments arranged by size:
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BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1717 2740 3454 4389 4588 5023 5417 5528
Size: 1717 1023 714 935 199 435 394 111
Cuts at: 5528 6458 7361 7413
Size: 930 903 52
Fragments arranged by size:
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199 111 52
BseYI C'CCAG_C
Cuts at: 0 252 2143 2243 3769 4252 4415 5082 5291
Size: 252 1891 100 1526 483 163 667 209
Cuts at: 5291 6584 6747 6965 7076 7110 7128 7413
Size: 1293 163 218 111 34 18 285
Fragments arranged by size:
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209 163 163 111 100 34 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 867 1218 4547 5469 5626 5720 7413
Size: 867 351 3329 922 157 94 1693
Fragments arranged by size:
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BsiEI CG_ry'CG
Cuts at: 0 1065 4460 7413
Size: 1065 3395 2953
Fragments arranged by size:
3395 2953 1065
BsiHKAI G_wGCw'C
Cuts at: 0 394 678 766 2586 2974 2983 3634 4076
Size: 394 284 88 1820 388 9 651 442
Cuts at: 4076 7413
Size: 3337
Fragments arranged by size:
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9
BsmI GAATG_Cn'
Cuts at: 0 907 1669 2173 2284 2535 3088 4489 5916
Size: 907 762 504 111 251 553 1401 1427
Cuts at: 5916 7413
Size: 1497
Fragments arranged by size:
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111
BsmBI CGTCTCn'nnnn_
Cuts at: 0 5689 7413
Size: 5689 1724
Bsp1286I G_dGCh'C
Cuts at: 0 394 628 671 678 766 1093 1164 2586
Size: 394 234 43 7 88 327 71 1422
Cuts at: 2586 2974 2983 3040 3302 3412 3634 3690 4076
Size: 388 9 57 262 110 222 56 386
Cuts at: 4076 4209 4704 5291 6260 6832 6899 6963 7003
Size: 133 495 587 969 572 67 64 40
Cuts at: 7003 7413
Size: 410
Fragments arranged by size:
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386 327 262 234 222 133 110 88
71 67 64 57 56 43 40 9
7
BspHI T'CATG_A
Cuts at: 0 6612 7413
Size: 6612 801
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 598 608 1063 1096 1213 1783 2434 2896
Size: 598 10 455 33 117 570 651 462
Cuts at: 2896 4736 5686 6988 7080 7392 7413
Size: 1840 950 1302 92 312 21
Fragments arranged by size:
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312 117 92 33 21 10
BsrBI CCG'CTC
Cuts at: 0 19 7413
Size: 19 7394
BsrDI GCAATG_nn'
Cuts at: 0 73 960 3699 7413
Size: 73 887 2739 3714
Fragments arranged by size:
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BsrFI r'CCGG_y
Cuts at: 0 3893 7413
Size: 3893 3520
BstAPI GCAn_nnn'nTGC
Cuts at: 0 75 1148 1844 2183 4071 4505 5761 6373
Size: 75 1073 696 339 1888 434 1256 612
Cuts at: 6373 7413
Size: 1040
Fragments arranged by size:
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75
BstBI TT'CG_AA
Cuts at: 0 2287 7413
Size: 2287 5126
BstEII G'GTnAC_C
Cuts at: 0 7216 7413
Size: 7216 197
BstXI CCAn_nnnn'nTGG
Cuts at: 0 226 583 3275 4351 6755 7413
Size: 226 357 2692 1076 2404 658
Fragments arranged by size:
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BstYI r'GATC_y
Cuts at: 0 3055 4822 6080 6265 6521 7413
Size: 3055 1767 1258 185 256 892
Fragments arranged by size:
3055 1767 1258 892 256 185
BstZ17I GTA'TAC
Cuts at: 0 3844 7413
Size: 3844 3569
Bsu36I CC'TnA_GG
Cuts at: 0 279 629 5429 7413
Size: 279 350 4800 1984
Fragments arranged by size:
4800 1984 350 279
BtgI C'CryG_G
Cuts at: 0 1259 1740 1965 2190 2652 4373 5362 5469
Size: 1259 481 225 225 462 1721 989 107
Cuts at: 5469 5925 7413
Size: 456 1488
Fragments arranged by size:
1721 1488 1259 989 481 462 456 225
225 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 5393 7413
Size: 5393 2020
BtsI GCAGTG_nn'
Cuts at: 0 85 570 1011 1528 1818 3498 4072 4911
Size: 85 485 441 517 290 1680 574 839
Cuts at: 4911 5756 5921 6554 6708 6714 7061 7413
Size: 845 165 633 154 6 347 352
Fragments arranged by size:
1680 845 839 633 574 517 485 441
352 347 290 165 154 85 6
ClaI AT'CG_AT
Cuts at: 0 1643 7413
Size: 1643 5770
DraI TTT'AAA
Cuts at: 0 2361 7413
Size: 2361 5052
DraIII CAC_nnn'GTG
Cuts at: 0 688 1168 2463 6690 7413
Size: 688 480 1295 4227 723
Fragments arranged by size:
4227 1295 723 688 480
DrdI GACnn_nn'nnGTC
Cuts at: 0 7374 7413
Size: 7374 39
EaeI y'GGCC_r
Cuts at: 0 485 1262 3891 3962 5359 6551 7413
Size: 485 777 2629 71 1397 1192 862
Fragments arranged by size:
2629 1397 1192 862 777 485 71
EarI CTCTTCn'nnn_
Cuts at: 0 3662 5153 5565 5945 6023 7413
Size: 3662 1491 412 380 78 1390
Fragments arranged by size:
3662 1491 1390 412 380 78
EcoICRI GAG'CTC
Cuts at: 0 2981 7413
Size: 2981 4432
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1008 1028 1354 2225 2691 3312 4660 5000
Size: 1008 20 326 871 466 621 1348 340
Cuts at: 5000 5156 5333 5590 6197 6301 6680 6803 6887
Size: 156 177 257 607 104 379 123 84
Cuts at: 6887 6971 7207 7364 7413
Size: 84 236 157 49
Fragments arranged by size:
1348 1008 871 621 607 466 379 340
326 257 236 177 157 156 123 104
84 84 49 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 771 3106 6358 7413
Size: 771 2335 3252 1055
Fragments arranged by size:
3252 2335 1055 771
EcoO109I rG'GnC_Cy
Cuts at: 0 1348 2131 2885 3654 3694 4970 5287 5288
Size: 1348 783 754 769 40 1276 317 1
Cuts at: 5288 7000 7360 7413
Size: 1712 360 53
Fragments arranged by size:
1712 1348 1276 783 769 754 360 317
53 40 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1973 2373 7413
Size: 1973 400 5040
Fragments arranged by size:
5040 1973 400
FspI TGC'GCA
Cuts at: 0 4732 7413
Size: 4732 2681
HaeII r_GCGC'y
Cuts at: 0 34 43 1316 1805 4179 7413
Size: 34 9 1273 489 2374 3234
Fragments arranged by size:
3234 2374 1273 489 34 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3445 3477 4108 4140 6219 6251 6257 6289
Size: 3445 32 631 32 2079 32 6 32
Cuts at: 6289 6478 6510 7258 7290 7413
Size: 189 32 748 32 123
Fragments arranged by size:
3445 2079 748 631 189 123 32 32
32 32 32 32 6
HincII GTy'rAC
Cuts at: 0 187 837 981 2904 3546 6227 7413
Size: 187 650 144 1923 642 2681 1186
Fragments arranged by size:
2681 1923 1186 650 642 187 144
HindIII A'AGCT_T
Cuts at: 0 6717 7413
Size: 6717 696
KasI G'GCGC_C
Cuts at: 0 1312 1801 7413
Size: 1312 489 5612
Fragments arranged by size:
5612 1312 489
KpnI G_GTAC'C
Cuts at: 0 941 3137 4637 4712 7413
Size: 941 2196 1500 75 2701
Fragments arranged by size:
2701 2196 1500 941 75
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3488 3833 6441 6509 7413
Size: 3488 345 2608 68 904
Fragments arranged by size:
3488 2608 904 345 68
MscI TGG'CCA
Cuts at: 0 487 1264 3964 6553 7413
Size: 487 777 2700 2589 860
Fragments arranged by size:
2700 2589 860 777 487
MslI CAynn'nnrTG
Cuts at: 0 78 455 1124 1440 1652 1690 3273 3368
Size: 78 377 669 316 212 38 1583 95
Cuts at: 3368 6292 6476 6772 7066 7172 7413
Size: 2924 184 296 294 106 241
Fragments arranged by size:
2924 1583 669 377 316 296 294 241
212 184 106 95 78 38
MspA1I CmG'CkG
Cuts at: 0 215 854 2114 2147 2604 3143 3149 4034
Size: 215 639 1260 33 457 539 6 885
Cuts at: 4034 4094 4163 4229 4792 5732 5758 5953 6823
Size: 60 69 66 563 940 26 195 870
Cuts at: 6823 6955 6991 7356 7413
Size: 132 36 365 57
Fragments arranged by size:
1260 940 885 870 639 563 539 457
365 215 195 132 69 66 60 57
36 33 26 6
NaeI GCC'GGC
Cuts at: 0 3895 7413
Size: 3895 3518
NarI GG'CG_CC
Cuts at: 0 1313 1802 7413
Size: 1313 489 5611
Fragments arranged by size:
5611 1313 489
NcoI C'CATG_G
Cuts at: 0 1259 1740 1965 2190 2652 4373 5362 5925
Size: 1259 481 225 225 462 1721 989 563
Cuts at: 5925 7413
Size: 1488
Fragments arranged by size:
1721 1488 1259 989 563 481 462 225
225
NdeI CA'TA_TG
Cuts at: 0 365 6122 7413
Size: 365 5757 1291
Fragments arranged by size:
5757 1291 365
NgoMIV G'CCGG_C
Cuts at: 0 3893 7413
Size: 3893 3520
NsiI A_TGCA'T
Cuts at: 0 75 1134 1138 7413
Size: 75 1059 4 6275
Fragments arranged by size:
6275 1059 75 4
NspI r_CATG'y
Cuts at: 0 73 362 1089 1136 1288 1439 1449 2090
Size: 73 289 727 47 152 151 10 641
Cuts at: 2090 2688 3005 3491 3975 4508 6015 6162 6592
Size: 598 317 486 484 533 1507 147 430
Cuts at: 6592 7377 7401 7413
Size: 785 24 12
Fragments arranged by size:
1507 785 727 641 598 533 486 484
430 317 289 152 151 147 73 47
24 12 10
PasI CC'CwG_GG
Cuts at: 0 2673 3156 3414 4385 4749 6517 7333 7413
Size: 2673 483 258 971 364 1768 816 80
Fragments arranged by size:
2673 1768 971 816 483 364 258 80
PciI A'CATG_T
Cuts at: 0 1435 2086 2684 3001 7373 7413
Size: 1435 651 598 317 4372 40
Fragments arranged by size:
4372 1435 651 598 317 40
PflMI CCAn_nnn'nTGG
Cuts at: 0 6703 7413
Size: 6703 710
PfoI T'CCnGG_A
Cuts at: 0 564 3328 4676 5687 7413
Size: 564 2764 1348 1011 1726
Fragments arranged by size:
2764 1726 1348 1011 564
PmlI CAC'GTG
Cuts at: 0 266 7413
Size: 266 7147
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4658 4690 7413
Size: 4658 32 2723
Fragments arranged by size:
4658 2723 32
PpuMI rG'GwC_Cy
Cuts at: 0 1348 2131 2885 3694 4970 7360 7413
Size: 1348 783 754 809 1276 2390 53
Fragments arranged by size:
2390 1348 1276 809 783 754 53
PshAI GACnn'nnGTC
Cuts at: 0 544 7413
Size: 544 6869
PspOMI G'GGCC_C
Cuts at: 0 5287 6256 6999 7413
Size: 5287 969 743 414
Fragments arranged by size:
5287 969 743 414
PstI C_TGCA'G
Cuts at: 0 914 1006 1813 3148 3202 3820 5391 5751
Size: 914 92 807 1335 54 618 1571 360
Cuts at: 5751 7413
Size: 1662
Fragments arranged by size:
1662 1571 1335 914 807 618 360 92
54
PvuII CAG'CTG
Cuts at: 0 2147 3149 4034 4163 4792 5758 5953 6823
Size: 2147 1002 885 129 629 966 195 870
Cuts at: 6823 6991 7413
Size: 168 422
Fragments arranged by size:
2147 1002 966 885 870 629 422 195
168 129
SacI G_AGCT'C
Cuts at: 0 2983 7413
Size: 2983 4430
SanDI GG'GwC_CC
Cuts at: 0 2131 3694 4970 7413
Size: 2131 1563 1276 2443
Fragments arranged by size:
2443 2131 1563 1276
SapI GCTCTTCn'nnn_
Cuts at: 0 5945 7413
Size: 5945 1468
ScaI AGT'ACT
Cuts at: 0 307 649 1614 7413
Size: 307 342 965 5799
Fragments arranged by size:
5799 965 342 307
SexAI A'CCwGG_T
Cuts at: 0 1871 7413
Size: 1871 5542
SfcI C'TryA_G
Cuts at: 0 910 1002 1809 1995 3144 3198 3741 3816
Size: 910 92 807 186 1149 54 543 75
Cuts at: 3816 5387 5747 7413
Size: 1571 360 1666
Fragments arranged by size:
1666 1571 1149 910 807 543 360 186
92 75 54
SfoI GGC'GCC
Cuts at: 0 1314 1803 7413
Size: 1314 489 5610
Fragments arranged by size:
5610 1314 489
SmaI CCC'GGG
Cuts at: 0 1791 3691 4004 4114 6055 6139 7413
Size: 1791 1900 313 110 1941 84 1274
Fragments arranged by size:
1941 1900 1791 1274 313 110 84
SmlI C'TyrA_G
Cuts at: 0 6720 7413
Size: 6720 693
SphI G_CATG'C
Cuts at: 0 73 1136 1288 1449 6162 6592 7413
Size: 73 1063 152 161 4713 430 821
Fragments arranged by size:
4713 1063 821 430 161 152 73
SspI AAT'ATT
Cuts at: 0 181 780 2385 7413
Size: 181 599 1605 5028
Fragments arranged by size:
5028 1605 599 181
StuI AGG'CCT
Cuts at: 0 4324 5323 6532 7413
Size: 4324 999 1209 881
Fragments arranged by size:
4324 1209 999 881
StyI C'CwwG_G
Cuts at: 0 1259 1740 1965 2190 2418 2652 4373 5362
Size: 1259 481 225 225 228 234 1721 989
Cuts at: 5362 5766 5925 6359 6377 7413
Size: 404 159 434 18 1036
Fragments arranged by size:
1721 1259 1036 989 481 434 404 234
228 225 225 159 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2686 3194 6552 6847 7218 7413
Size: 2686 508 3358 295 371 195
Fragments arranged by size:
3358 2686 508 371 295 195
TatI w'GTAC_w
Cuts at: 0 305 647 1039 1612 2200 2341 6788 7413
Size: 305 342 392 573 588 141 4447 625
Fragments arranged by size:
4447 625 588 573 392 342 305 141
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 753 785 1283 1315 3420 3452 5828 5860
Size: 753 32 498 32 2105 32 2376 32
Cuts at: 5860 6483 6515 6636 6668 6969 7001 7413
Size: 623 32 121 32 301 32 412
Fragments arranged by size:
2376 2105 753 623 498 412 301 121
32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1873 2899 4974 5584 7413
Size: 1873 1026 2075 610 1829
Fragments arranged by size:
2075 1873 1829 1026 610
XbaI T'CTAG_A
Cuts at: 0 5808 6307 7413
Size: 5808 499 1106
Fragments arranged by size:
5808 1106 499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3706 4033 4412 5685 6546 7413
Size: 3706 327 379 1273 861 867
Fragments arranged by size:
3706 1273 867 861 379 327
XmaI C'CCGG_G
Cuts at: 0 1789 3689 4002 4112 6053 6137 7413
Size: 1789 1900 313 110 1941 84 1276
Fragments arranged by size:
1941 1900 1789 1276 313 110 84
XmnI GAAnn'nnTTC
Cuts at: 0 2107 2794 3622 7413
Size: 2107 687 828 3791
Fragments arranged by size:
3791 2107 828 687
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuII SacI SanDI SapI ScaI SexAI SfcI SfoI
SmaI SmlI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AscI AsiSI AvrII
BmtI BsiWI BspEI BsrGI BssHII BssSI CspCI EagI
EciI EcoRI EcoRV FseI FspAI HpaI MfeI MluI
NheI NotI NruI PacI PmeI PsiI PspXI PsrI
PvuI RsrII SacII SalI SbfI SfiI SgfI SgrAI
SnaBI SpeI SrfI SwaI XhoI ZraI