Restriction map for
pF1KE3483
(Linear) (Six-base) MAPSORT of: KIEE3483.seq Check: 5062 from: 1 to: 5238
KIEE3483.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4003 4464 5238
Size: 4003 461 774
Fragments arranged by size:
4003 774 461
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 807 2507 2753 3206 3963 4208 4283
Size: 470 337 1700 246 453 757 245 75
Cuts at: 4283 4334 4864 5238
Size: 51 530 374
Fragments arranged by size:
1700 757 530 470 453 374 337 246
245 75 51
AfeI AGC'GCT
Cuts at: 0 2770 5238
Size: 2770 2468
AflIII A'CryG_T
Cuts at: 0 27 5238
Size: 27 5211
AhdI GACnn_n'nnGTC
Cuts at: 0 3636 3969 5238
Size: 3636 333 1269
Fragments arranged by size:
3636 1269 333
AleI CACnn'nnGTG
Cuts at: 0 646 5238
Size: 646 4592
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1658 1690 5238
Size: 1658 32 3548
Fragments arranged by size:
3548 1658 32
AlwNI CAG_nnn'CTG
Cuts at: 0 97 2407 2713 4140 4920 4995 5238
Size: 97 2310 306 1427 780 75 243
Fragments arranged by size:
2310 1427 780 306 243 97 75
ApaI G_GGCC'C
Cuts at: 0 1474 3779 5008 5238
Size: 1474 2305 1229 230
Fragments arranged by size:
2305 1474 1229 230
ApoI r'AATT_y
Cuts at: 0 966 1780 5222 5238
Size: 966 814 3442 16
Fragments arranged by size:
3442 966 814 16
AvaI C'yCGr_G
Cuts at: 0 1465 3173 4702 4904 5238
Size: 1465 1708 1529 202 334
Fragments arranged by size:
1708 1529 1465 334 202
BamHI G'GATC_C
Cuts at: 0 3177 4218 4958 5238
Size: 3177 1041 740 280
Fragments arranged by size:
3177 1041 740 280
BanI G'GyrC_C
Cuts at: 0 105 5238
Size: 105 5133
BanII G_rGCy'C
Cuts at: 0 1154 1474 2101 3779 4622 5008 5238
Size: 1154 320 627 1678 843 386 230
Fragments arranged by size:
1678 1154 843 627 386 320 230
BbsI GAAGACnn'nnnn_
Cuts at: 0 2047 2144 2955 3281 3923 4274 5238
Size: 2047 97 811 326 642 351 964
Fragments arranged by size:
2047 964 811 642 351 326 97
BbvCI CC'TCA_GC
Cuts at: 0 3687 5238
Size: 3687 1551
BciVI GTATCCnnnnn_n'
Cuts at: 0 471 5238
Size: 471 4767
BclI T'GATC_A
Cuts at: 0 3476 5108 5238
Size: 3476 1632 130
Fragments arranged by size:
3476 1632 130
BfrBI ATG'CAT
Cuts at: 0 1900 3067 5238
Size: 1900 1167 2171
Fragments arranged by size:
2171 1900 1167
BglI GCCn_nnn'nGGC
Cuts at: 0 2445 2867 5238
Size: 2445 422 2371
Fragments arranged by size:
2445 2371 422
BglII A'GATC_T
Cuts at: 0 4076 5016 5238
Size: 4076 940 222
Fragments arranged by size:
4076 940 222
BlpI GC'TnA_GC
Cuts at: 0 265 5238
Size: 265 4973
Bme1580I G_kGCm'C
Cuts at: 0 1474 1563 3779 5008 5238
Size: 1474 89 2216 1229 230
Fragments arranged by size:
2216 1474 1229 230 89
BmrI ACTGGGnnnn_n'
Cuts at: 0 3588 4749 5238
Size: 3588 1161 489
Fragments arranged by size:
3588 1161 489
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2228 3519 5238
Size: 2228 1291 1719
Fragments arranged by size:
2228 1719 1291
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 498 1505 2324 2384 2882 3747 4972 5238
Size: 498 1007 819 60 498 865 1225 266
Fragments arranged by size:
1225 1007 865 819 498 498 266 60
Bpu10I CC'TnA_GC
Cuts at: 0 389 2765 3687 5238
Size: 389 2376 922 1551
Fragments arranged by size:
2376 1551 922 389
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 2198 2972 3859 4531 5176 5238
Size: 351 1847 774 887 672 645 62
Fragments arranged by size:
1847 887 774 672 645 351 62
BsaI GGTCTCn'nnnn_
Cuts at: 0 981 1225 3669 5238
Size: 981 244 2444 1569
Fragments arranged by size:
2444 1569 981 244
BsaHI Gr'CG_yC
Cuts at: 0 4271 4973 5238
Size: 4271 702 265
Fragments arranged by size:
4271 702 265
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 825 855 1720 1750 2544 2574 2616 2646
Size: 825 30 865 30 794 30 42 30
Cuts at: 2646 5238
Size: 2592
Fragments arranged by size:
2592 865 825 794 42 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 715 751 754 2327 2830 2882 2885 3108
Size: 715 36 3 1573 503 52 3 223
Cuts at: 3108 3161 4380 5238
Size: 53 1219 858
Fragments arranged by size:
1573 1219 858 715 503 223 53 52
36 3 3
BseYI C'CCAG_C
Cuts at: 0 1330 2190 3931 4439 4621 5238
Size: 1330 860 1741 508 182 617
Fragments arranged by size:
1741 1330 860 617 508 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3201 4792 5238
Size: 3201 1591 446
Fragments arranged by size:
3201 1591 446
BsiHKAI G_wGCw'C
Cuts at: 0 643 746 1463 2425 4517 5175 5238
Size: 643 103 717 962 2092 658 63
Fragments arranged by size:
2092 962 717 658 643 103 63
BsmI GAATG_Cn'
Cuts at: 0 52 995 3069 5238
Size: 52 943 2074 2169
Fragments arranged by size:
2169 2074 943 52
Bsp1286I G_dGCh'C
Cuts at: 0 643 746 1154 1463 1474 1563 2101 2425
Size: 643 103 408 309 11 89 538 324
Cuts at: 2425 3779 4517 4622 5008 5175 5238
Size: 1354 738 105 386 167 63
Fragments arranged by size:
1354 738 643 538 408 386 324 309
167 105 103 89 63 11
BspHI T'CATG_A
Cuts at: 0 1830 4349 5105 5238
Size: 1830 2519 756 133
Fragments arranged by size:
2519 1830 756 133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2635 4003 4464 4571 4880 5238
Size: 2635 1368 461 107 309 358
Fragments arranged by size:
2635 1368 461 358 309 107
BsrBI CCG'CTC
Cuts at: 0 4892 4956 5238
Size: 4892 64 282
Fragments arranged by size:
4892 282 64
BsrDI GCAATG_nn'
Cuts at: 0 129 5238
Size: 129 5109
BsrFI r'CCGG_y
Cuts at: 0 668 3767 5238
Size: 668 3099 1471
Fragments arranged by size:
3099 1471 668
BsrGI T'GTAC_A
Cuts at: 0 1287 2969 5238
Size: 1287 1682 2269
Fragments arranged by size:
2269 1682 1287
BssSI C'ACGA_G
Cuts at: 0 745 4862 5238
Size: 745 4117 376
Fragments arranged by size:
4117 745 376
BstAPI GCAn_nnn'nTGC
Cuts at: 0 56 1249 2755 3064 3215 3436 4018 5238
Size: 56 1193 1506 309 151 221 582 1220
Fragments arranged by size:
1506 1220 1193 582 309 221 151 56
BstEII G'GTnAC_C
Cuts at: 0 1114 2647 5238
Size: 1114 1533 2591
Fragments arranged by size:
2591 1533 1114
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3750 4606 5238
Size: 3750 856 632
Fragments arranged by size:
3750 856 632
BstYI r'GATC_y
Cuts at: 0 2198 3177 3285 4050 4076 4218 4706 4958
Size: 2198 979 108 765 26 142 488 252
Cuts at: 4958 5016 5238
Size: 58 222
Fragments arranged by size:
2198 979 765 488 252 222 142 108
58 26
BtgI C'CryG_G
Cuts at: 0 117 782 1448 3524 5238
Size: 117 665 666 2076 1714
Fragments arranged by size:
2076 1714 666 665 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3384 5238
Size: 3384 1854
BtsI GCAGTG_nn'
Cuts at: 0 2379 3460 3597 4539 5098 5238
Size: 2379 1081 137 942 559 140
Fragments arranged by size:
2379 1081 942 559 140 137
DraI TTT'AAA
Cuts at: 0 63 2784 5238
Size: 63 2721 2454
Fragments arranged by size:
2721 2454 63
DraIII CAC_nnn'GTG
Cuts at: 0 4377 5238
Size: 4377 861
EaeI y'GGCC_r
Cuts at: 0 779 1451 2225 4922 5238
Size: 779 672 774 2697 316
Fragments arranged by size:
2697 779 774 672 316
EarI CTCTTCn'nnn_
Cuts at: 0 704 764 1259 1361 2375 3085 4250 4678
Size: 704 60 495 102 1014 710 1165 428
Cuts at: 4678 5026 5238
Size: 348 212
Fragments arranged by size:
1165 1014 710 704 495 428 348 212
102 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2785 5238
Size: 2785 2453
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 498 807 1505 2324 2384 2507 2753
Size: 470 28 309 698 819 60 123 246
Cuts at: 2753 2882 3206 3747 3963 4208 4283 4334 4864
Size: 129 324 541 216 245 75 51 530
Cuts at: 4864 4972 5238
Size: 108 266
Fragments arranged by size:
819 698 541 530 470 324 309 266
246 245 216 129 123 108 75 60
51 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2987 3654 5238
Size: 2987 667 1584
Fragments arranged by size:
2987 1584 667
EcoO109I rG'GnC_Cy
Cuts at: 0 1470 1471 3510 3776 5004 5005 5238
Size: 1470 1 2039 266 1228 1 233
Fragments arranged by size:
2039 1470 1228 266 233 1 1
EcoRV GAT'ATC
Cuts at: 0 2777 3567 5238
Size: 2777 790 1671
Fragments arranged by size:
2777 1671 790
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 75 1264 2006 4072 5238
Size: 75 1189 742 2066 1166
Fragments arranged by size:
2066 1189 1166 742 75
FspI TGC'GCA
Cuts at: 0 3667 5238
Size: 3667 1571
HaeII r_GCGC'y
Cuts at: 0 2772 2910 5238
Size: 2772 138 2328
Fragments arranged by size:
2772 2328 138
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1009 1041 3120 3152 3622 3654 3955 3987
Size: 1009 32 2079 32 470 32 301 32
Cuts at: 3987 5238
Size: 1251
Fragments arranged by size:
2079 1251 1009 470 301 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1479 2705 5238
Size: 1479 1226 2533
Fragments arranged by size:
2533 1479 1226
HindIII A'AGCT_T
Cuts at: 0 571 2366 2787 5238
Size: 571 1795 421 2451
Fragments arranged by size:
2451 1795 571 421
HpaI GTT'AAC
Cuts at: 0 1479 5238
Size: 1479 3759
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2346 2782 3767 5238
Size: 2346 436 985 1471
Fragments arranged by size:
2346 1471 985 436
MscI TGG'CCA
Cuts at: 0 781 1453 2227 4924 5238
Size: 781 672 774 2697 314
Fragments arranged by size:
2697 781 774 672 314
MslI CAynn'nnrTG
Cuts at: 0 122 646 1991 2881 3884 5238
Size: 122 524 1345 890 1003 1354
Fragments arranged by size:
1354 1345 1003 890 524 122
MspA1I CmG'CkG
Cuts at: 0 2752 3968 4920 4928 5238
Size: 2752 1216 952 8 310
Fragments arranged by size:
2752 1216 952 310 8
NaeI GCC'GGC
Cuts at: 0 3769 5238
Size: 3769 1469
NcoI C'CATG_G
Cuts at: 0 117 782 1448 3524 5238
Size: 117 665 666 2076 1714
Fragments arranged by size:
2076 1714 666 665 117
NdeI CA'TA_TG
Cuts at: 0 1902 4041 5238
Size: 1902 2139 1197
Fragments arranged by size:
2139 1902 1197
NgoMIV G'CCGG_C
Cuts at: 0 3767 5238
Size: 3767 1471
NsiI A_TGCA'T
Cuts at: 0 1902 3069 5238
Size: 1902 1167 2169
Fragments arranged by size:
2169 1902 1167
NspI r_CATG'y
Cuts at: 0 31 625 2886 5238
Size: 31 594 2261 2352
Fragments arranged by size:
2352 2261 594 31
PasI CC'CwG_GG
Cuts at: 0 4211 5238
Size: 4211 1027
PciI A'CATG_T
Cuts at: 0 27 5238
Size: 27 5211
PflMI CCAn_nnn'nTGG
Cuts at: 0 4377 4695 5238
Size: 4377 318 543
Fragments arranged by size:
4377 543 318
PfoI T'CCnGG_A
Cuts at: 0 4712 5238
Size: 4712 526
PpuMI rG'GwC_Cy
Cuts at: 0 3510 5238
Size: 3510 1728
PshAI GACnn'nnGTC
Cuts at: 0 4172 5238
Size: 4172 1066
PsiI TTA'TAA
Cuts at: 0 1793 5238
Size: 1793 3445
PspOMI G'GGCC_C
Cuts at: 0 1470 3775 5004 5238
Size: 1470 2305 1229 234
Fragments arranged by size:
2305 1470 1229 234
PspXI vC'TCGA_Gb
Cuts at: 0 1465 5238
Size: 1465 3773
PstI C_TGCA'G
Cuts at: 0 905 4617 4916 5231 5238
Size: 905 3712 299 315 7
Fragments arranged by size:
3712 905 315 299 7
PvuII CAG'CTG
Cuts at: 0 2752 3968 4920 4928 5238
Size: 2752 1216 952 8 310
Fragments arranged by size:
2752 1216 952 310 8
SapI GCTCTTCn'nnn_
Cuts at: 0 764 1361 2375 4678 5238
Size: 764 597 1014 2303 560
Fragments arranged by size:
2303 1014 764 597 560
SexAI A'CCwGG_T
Cuts at: 0 39 2698 5238
Size: 39 2659 2540
Fragments arranged by size:
2659 2540 39
SfcI C'TryA_G
Cuts at: 0 209 901 1319 2169 3073 4613 4912 5195
Size: 209 692 418 850 904 1540 299 283
Cuts at: 5195 5227 5238
Size: 32 11
Fragments arranged by size:
1540 904 850 692 418 299 283 209
32 11
SmaI CCC'GGG
Cuts at: 0 4906 5238
Size: 4906 332
SmlI C'TyrA_G
Cuts at: 0 330 1465 2213 2987 3838 4510 5155 5238
Size: 330 1135 748 774 851 672 645 83
Fragments arranged by size:
1135 851 774 748 672 645 330 83
SpeI A'CTAG_T
Cuts at: 0 1654 5238
Size: 1654 3584
StuI AGG'CCT
Cuts at: 0 3660 5238
Size: 3660 1578
StyI C'CwwG_G
Cuts at: 0 117 782 1448 1681 3377 3506 3524 3650
Size: 117 665 666 233 1696 129 18 126
Cuts at: 3650 4599 5238
Size: 949 639
Fragments arranged by size:
1696 949 666 665 639 233 129 126
117 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 123 130 1731 3447 5238
Size: 123 7 1601 1716 1791
Fragments arranged by size:
1791 1716 1601 123 7
TatI w'GTAC_w
Cuts at: 0 717 1287 2523 2969 5052 5238
Size: 717 570 1236 446 2083 186
Fragments arranged by size:
2083 1236 717 570 446 186
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 59 825 857 2493 2525 2544 2576
Size: 27 32 766 32 1636 32 19 32
Cuts at: 2576 5238
Size: 2662
Fragments arranged by size:
2662 1636 766 32 32 32 32 27
19
XbaI T'CTAG_A
Cuts at: 0 3337 5238
Size: 3337 1901
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 231 3384 3952 4582 5238
Size: 231 3153 568 630 656
Fragments arranged by size:
3153 656 630 568 231
XhoI C'TCGA_G
Cuts at: 0 1465 5238
Size: 1465 3773
XmaI C'CCGG_G
Cuts at: 0 4904 5238
Size: 4904 334
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AloI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII EaeI EarI EciI
Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PpuMI PshAI PsiI PspOMI PspXI PstI PvuII
SapI SexAI SfcI SmaI SmlI SpeI StuI StyI
TaqII TatI TstI XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AlfI ApaLI
AscI AseI AsiSI AvrII BaeI BbeI BcgI BmgBI
BmtI BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI
BssHII BstBI BstZ17I Bsu36I ClaI CspCI DrdI EagI
EcoICRI EcoRI FseI FspAI KasI KpnI MfeI MluI
NarI NheI NotI NruI PacI PmeI PmlI PpiI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SnaBI SphI SrfI
SspI SwaI Tth111I XmnI ZraI