Restriction map for
pF1KE3426
(Linear) (Six-base) MAPSORT of: KIEE3426.seq Check: 2304 from: 1 to: 1779
KIEE3426.orf
With 182 enzymes: SgfI *
October 12, 2011 16:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1665 1752 1779
Size: 1665 87 27
Fragments arranged by size:
1665 87 27
AatII G_ACGT'C
Cuts at: 0 92 1779
Size: 92 1687
Acc65I G'GTAC_C
Cuts at: 0 305 1779
Size: 305 1474
AleI CACnn'nnGTG
Cuts at: 0 1231 1508 1510 1779
Size: 1231 277 2 269
Fragments arranged by size:
1231 277 269 2
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 98 130 1669 1701 1779
Size: 98 32 1539 32 78
Fragments arranged by size:
1539 98 78 32 32
ApoI r'AATT_y
Cuts at: 0 514 1779
Size: 514 1265
AvaI C'yCGr_G
Cuts at: 0 1642 1779
Size: 1642 137
BanI G'GyrC_C
Cuts at: 0 288 305 314 363 468 739 798 1092
Size: 288 17 9 49 105 271 59 294
Cuts at: 1092 1779
Size: 687
Fragments arranged by size:
687 294 288 271 105 59 49 17
9
BanII G_rGCy'C
Cuts at: 0 1066 1779
Size: 1066 713
BbeI G_GCGC'C
Cuts at: 0 318 743 802 1779
Size: 318 425 59 977
Fragments arranged by size:
977 425 318 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 969 993 1260 1779
Size: 969 24 267 519
Fragments arranged by size:
969 519 267 24
BbvCI CC'TCA_GC
Cuts at: 0 1552 1595 1779
Size: 1552 43 184
Fragments arranged by size:
1552 184 43
BglI GCCn_nnn'nGGC
Cuts at: 0 1737 1779
Size: 1737 42
BglII A'GATC_T
Cuts at: 0 110 1779
Size: 110 1669
BlpI GC'TnA_GC
Cuts at: 0 55 758 1779
Size: 55 703 1021
Fragments arranged by size:
1021 703 55
Bme1580I G_kGCm'C
Cuts at: 0 366 389 479 1097 1779
Size: 366 23 90 618 682
Fragments arranged by size:
682 618 366 90 23
BmgBI CAC'GTC
Cuts at: 0 488 1779
Size: 488 1291
BmrI ACTGGGnnnn_n'
Cuts at: 0 132 1779
Size: 132 1647
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1740 1779
Size: 1740 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1654 1779
Size: 1654 125
Bpu10I CC'TnA_GC
Cuts at: 0 131 1552 1595 1704 1733 1779
Size: 131 1421 43 109 29 46
Fragments arranged by size:
1421 131 109 46 43 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 1080 1779
Size: 139 941 699
Fragments arranged by size:
941 699 139
BsaI GGTCTCn'nnnn_
Cuts at: 0 1165 1299 1630 1639 1779
Size: 1165 134 331 9 140
Fragments arranged by size:
1165 331 140 134 9
BsaBI GATnn'nnATC
Cuts at: 0 207 1779
Size: 207 1572
BsaHI Gr'CG_yC
Cuts at: 0 89 315 598 740 799 1253 1779
Size: 89 226 283 142 59 454 526
Fragments arranged by size:
526 454 283 226 142 89 59
BsaWI w'CCGG_w
Cuts at: 0 393 1208 1779
Size: 393 815 571
Fragments arranged by size:
815 571 393
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 895 925 1048 1078 1779
Size: 895 30 123 30 701
Fragments arranged by size:
895 701 123 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 289 787 790 1732 1779
Size: 289 498 3 942 47
Fragments arranged by size:
942 498 289 47 3
BseYI C'CCAG_C
Cuts at: 0 357 458 983 1052 1626 1779
Size: 357 101 525 69 574 153
Fragments arranged by size:
574 525 357 153 101 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 628 633 901 1779
Size: 106 522 5 268 878
Fragments arranged by size:
878 522 268 106 5
BsiEI CG_ry'CG
Cuts at: 0 1185 1779
Size: 1185 594
BsiHKAI G_wGCw'C
Cuts at: 0 56 132 620 731 1066 1118 1154 1238
Size: 56 76 488 111 335 52 36 84
Cuts at: 1238 1779
Size: 541
Fragments arranged by size:
541 488 335 111 84 76 56 52
36
BsiWI C'GTAC_G
Cuts at: 0 65 1779
Size: 65 1714
Bsp1286I G_dGCh'C
Cuts at: 0 56 132 366 389 479 620 731 1066
Size: 56 76 234 23 90 141 111 335
Cuts at: 1066 1097 1118 1154 1238 1779
Size: 31 21 36 84 541
Fragments arranged by size:
541 335 234 141 111 90 84 76
56 36 31 23 21
BspEI T'CCGG_A
Cuts at: 0 393 1779
Size: 393 1386
BspHI T'CATG_A
Cuts at: 0 92 1779
Size: 92 1687
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 293 923 1665 1752 1779
Size: 293 630 742 87 27
Fragments arranged by size:
742 630 293 87 27
BsrBI CCG'CTC
Cuts at: 0 1737 1779
Size: 1737 42
BsrDI GCAATG_nn'
Cuts at: 0 456 1779
Size: 456 1323
BsrFI r'CCGG_y
Cuts at: 0 826 1180 1184 1779
Size: 826 354 4 595
Fragments arranged by size:
826 595 354 4
BssHII G'CGCG_C
Cuts at: 0 696 783 1779
Size: 696 87 996
Fragments arranged by size:
996 696 87
BssSI C'ACGA_G
Cuts at: 0 533 1779
Size: 533 1246
BstXI CCAn_nnnn'nTGG
Cuts at: 0 186 1779
Size: 186 1593
BstYI r'GATC_y
Cuts at: 0 110 1779
Size: 110 1669
BtgI C'CryG_G
Cuts at: 0 575 1493 1661 1779
Size: 575 918 168 118
Fragments arranged by size:
918 575 168 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 774 1779
Size: 774 1005
BtsI GCAGTG_nn'
Cuts at: 0 645 1571 1779
Size: 645 926 208
Fragments arranged by size:
926 645 208
DraIII CAC_nnn'GTG
Cuts at: 0 370 1049 1779
Size: 370 679 730
Fragments arranged by size:
730 679 370
DrdI GACnn_nn'nnGTC
Cuts at: 0 213 1779
Size: 213 1566
EaeI y'GGCC_r
Cuts at: 0 352 875 1037 1182 1274 1587 1605 1738
Size: 352 523 162 145 92 313 18 133
Cuts at: 1738 1779
Size: 41
Fragments arranged by size:
523 352 313 162 145 133 92 41
18
EagI C'GGCC_G
Cuts at: 0 1182 1779
Size: 1182 597
EarI CTCTTCn'nnn_
Cuts at: 0 1135 1342 1754 1779
Size: 1135 207 412 25
Fragments arranged by size:
1135 412 207 25
EcoICRI GAG'CTC
Cuts at: 0 1064 1779
Size: 1064 715
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1654 1779
Size: 1654 125
EcoNI CCTnn'n_nnAGG
Cuts at: 0 633 769 1714 1753 1779
Size: 633 136 945 39 26
Fragments arranged by size:
945 633 136 39 26
EcoO109I rG'GnC_Cy
Cuts at: 0 162 1622 1779
Size: 162 1460 157
Fragments arranged by size:
1460 162 157
HaeII r_GCGC'y
Cuts at: 0 34 318 743 765 802 1779
Size: 34 284 425 22 37 977
Fragments arranged by size:
977 425 284 37 34 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 232 264 1048 1080 1346 1378 1779
Size: 232 32 784 32 266 32 401
Fragments arranged by size:
784 401 266 232 32 32 32
KasI G'GCGC_C
Cuts at: 0 314 739 798 1779
Size: 314 425 59 981
Fragments arranged by size:
981 425 314 59
KpnI G_GTAC'C
Cuts at: 0 309 1779
Size: 309 1470
MscI TGG'CCA
Cuts at: 0 877 1039 1276 1607 1779
Size: 877 162 237 331 172
Fragments arranged by size:
877 331 237 172 162
MslI CAynn'nnrTG
Cuts at: 0 667 931 1090 1231 1508 1510 1779
Size: 667 264 159 141 277 2 269
Fragments arranged by size:
667 277 269 264 159 141 2
MspA1I CmG'CkG
Cuts at: 0 357 458 823 997 1427 1466 1599 1779
Size: 357 101 365 174 430 39 133 180
Fragments arranged by size:
430 365 357 180 174 133 101 39
NaeI GCC'GGC
Cuts at: 0 828 1182 1779
Size: 828 354 597
Fragments arranged by size:
828 597 354
NarI GG'CG_CC
Cuts at: 0 315 740 799 1779
Size: 315 425 59 980
Fragments arranged by size:
980 425 315 59
NcoI C'CATG_G
Cuts at: 0 1493 1661 1779
Size: 1493 168 118
Fragments arranged by size:
1493 168 118
NgoMIV G'CCGG_C
Cuts at: 0 826 1180 1779
Size: 826 354 599
Fragments arranged by size:
826 599 354
NspI r_CATG'y
Cuts at: 0 672 1779
Size: 672 1107
PfoI T'CCnGG_A
Cuts at: 0 1066 1779
Size: 1066 713
PpuMI rG'GwC_Cy
Cuts at: 0 162 1622 1779
Size: 162 1460 157
Fragments arranged by size:
1460 162 157
PshAI GACnn'nnGTC
Cuts at: 0 1519 1779
Size: 1519 260
PstI C_TGCA'G
Cuts at: 0 170 844 1779
Size: 170 674 935
Fragments arranged by size:
935 674 170
PvuII CAG'CTG
Cuts at: 0 458 1599 1779
Size: 458 1141 180
Fragments arranged by size:
1141 458 180
SacI G_AGCT'C
Cuts at: 0 1066 1779
Size: 1066 713
SanDI GG'GwC_CC
Cuts at: 0 1622 1779
Size: 1622 157
SapI GCTCTTCn'nnn_
Cuts at: 0 1342 1779
Size: 1342 437
SexAI A'CCwGG_T
Cuts at: 0 366 1779
Size: 366 1413
SfcI C'TryA_G
Cuts at: 0 166 498 840 1479 1753 1779
Size: 166 332 342 639 274 26
Fragments arranged by size:
639 342 332 274 166 26
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1737 1779
Size: 1737 42
SfoI GGC'GCC
Cuts at: 0 316 741 800 1779
Size: 316 425 59 979
Fragments arranged by size:
979 425 316 59
SmlI C'TyrA_G
Cuts at: 0 154 1059 1642 1779
Size: 154 905 583 137
Fragments arranged by size:
905 583 154 137
StuI AGG'CCT
Cuts at: 0 1709 1723 1779
Size: 1709 14 56
Fragments arranged by size:
1709 56 14
StyI C'CwwG_G
Cuts at: 0 1493 1661 1673 1779
Size: 1493 168 12 106
Fragments arranged by size:
1493 168 106 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 306 1779
Size: 306 1473
XhoI C'TCGA_G
Cuts at: 0 1642 1779
Size: 1642 137
ZraI GAC'GTC
Cuts at: 0 90 1779
Size: 90 1689
Enzymes that do cut:
AarI AatII Acc65I AleI AloI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstXI BstYI BtgI BtgZI BtsI DraIII
DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I KasI KpnI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PfoI PpuMI PshAI PstI
PvuII SacI SanDI SapI SexAI SfcI SfiI SfoI
SmlI StuI StyI TaqII XhoI ZraI
Enzymes that do not cut:
AccI AclI AcuI AfeI AflII AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BclI BfrBI BmtI BsaAI
BsmI BsmBI BsrGI BstAPI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI EciI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI MfeI MluI MmeI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SbfI ScaI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI
TstI Tth111I XbaI XcmI XmaI XmnI