Restriction map for
pF1KE3356
(Linear) (Six-base) MAPSORT of: KIEE3356.seq Check: 6613 from: 1 to: 3144
KIEE3356.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1722 3144
Size: 1722 1422
AccI GT'mk_AC
Cuts at: 0 2253 3144
Size: 2253 891
Acc65I G'GTAC_C
Cuts at: 0 733 3144
Size: 733 2411
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1343 1613 1646 2399 2473 2779 3144
Size: 1343 270 33 753 74 306 365
Fragments arranged by size:
1343 753 365 306 270 74 33
AfeI AGC'GCT
Cuts at: 0 1767 3144
Size: 1767 1377
AflII C'TTAA_G
Cuts at: 0 1726 3144
Size: 1726 1418
AflIII A'CryG_T
Cuts at: 0 246 476 1255 3144
Size: 246 230 779 1889
Fragments arranged by size:
1889 779 246 230
AleI CACnn'nnGTG
Cuts at: 0 2995 3144
Size: 2995 149
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2034 3144
Size: 2034 1110
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 187 219 333 365 3144
Size: 187 32 114 32 2779
Fragments arranged by size:
2779 187 114 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2190 2895 3144
Size: 2190 705 249
Fragments arranged by size:
2190 705 249
ApaLI G'TGCA_C
Cuts at: 0 613 709 3144
Size: 613 96 2435
Fragments arranged by size:
2435 613 96
ApoI r'AATT_y
Cuts at: 0 379 1504 1665 2061 2731 3099 3144
Size: 379 1125 161 396 670 368 45
Fragments arranged by size:
1125 670 396 379 368 161 45
AvaI C'yCGr_G
Cuts at: 0 780 2569 2794 3144
Size: 780 1789 225 350
Fragments arranged by size:
1789 780 350 225
BamHI G'GATC_C
Cuts at: 0 1089 3144
Size: 1089 2055
BanI G'GyrC_C
Cuts at: 0 733 1687 2676 3144
Size: 733 954 989 468
Fragments arranged by size:
989 954 733 468
BanII G_rGCy'C
Cuts at: 0 2079 2386 2744 2923 3144
Size: 2079 307 358 179 221
Fragments arranged by size:
2079 358 307 221 179
BbsI GAAGACnn'nnnn_
Cuts at: 0 239 1331 1537 3144
Size: 239 1092 206 1607
Fragments arranged by size:
1607 1092 239 206
BbvCI CC'TCA_GC
Cuts at: 0 863 3144
Size: 863 2281
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1013 1047 1570 1604 3005 3039 3144
Size: 1013 34 523 34 1401 34 105
Fragments arranged by size:
1401 1013 523 105 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2891 3144
Size: 2891 253
BfrBI ATG'CAT
Cuts at: 0 857 3144
Size: 857 2287
Bme1580I G_kGCm'C
Cuts at: 0 617 713 2679 3144
Size: 617 96 1966 465
Fragments arranged by size:
1966 617 465 96
BmgBI CAC'GTC
Cuts at: 0 1256 3144
Size: 1256 1888
BmrI ACTGGGnnnn_n'
Cuts at: 0 1097 3144
Size: 1097 2047
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 648 3144
Size: 648 2496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1164 2212 2907 3144
Size: 1164 1048 695 237
Fragments arranged by size:
1164 1048 695 237
Bpu10I CC'TnA_GC
Cuts at: 0 863 2917 3144
Size: 863 2054 227
Fragments arranged by size:
2054 863 227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 831 3144
Size: 831 2313
BsaI GGTCTCn'nnnn_
Cuts at: 0 807 1999 3144
Size: 807 1192 1145
Fragments arranged by size:
1192 1145 807
BsaWI w'CCGG_w
Cuts at: 0 1199 1950 2260 2550 3144
Size: 1199 751 310 290 594
Fragments arranged by size:
1199 751 594 310 290
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 333 363 490 520 741 771 1245 1275
Size: 333 30 127 30 221 30 474 30
Cuts at: 1275 1932 1962 3144
Size: 657 30 1182
Fragments arranged by size:
1182 657 474 333 221 127 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 624 1934 1937 3144
Size: 624 1310 3 1207
Fragments arranged by size:
1310 1207 624 3
BseYI C'CCAG_C
Cuts at: 0 2280 3144
Size: 2280 864
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1043 2531 3144
Size: 1043 1488 613
Fragments arranged by size:
1488 1043 613
BsiEI CG_ry'CG
Cuts at: 0 623 3144
Size: 623 2521
BsiHKAI G_wGCw'C
Cuts at: 0 617 701 713 964 2923 3144
Size: 617 84 12 251 1959 221
Fragments arranged by size:
1959 617 251 221 84 12
BsmI GAATG_Cn'
Cuts at: 0 776 3117 3144
Size: 776 2341 27
Fragments arranged by size:
2341 776 27
Bsp1286I G_dGCh'C
Cuts at: 0 617 701 713 964 2079 2386 2679 2744
Size: 617 84 12 251 1115 307 293 65
Cuts at: 2744 2923 3144
Size: 179 221
Fragments arranged by size:
1115 617 307 293 251 221 179 84
65 12
BspEI T'CCGG_A
Cuts at: 0 1950 3144
Size: 1950 1194
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1722 3144
Size: 1722 1422
BsrFI r'CCGG_y
Cuts at: 0 263 1207 3144
Size: 263 944 1937
Fragments arranged by size:
1937 944 263
BsrGI T'GTAC_A
Cuts at: 0 47 3144
Size: 47 3097
BssHII G'CGCG_C
Cuts at: 0 743 3144
Size: 743 2401
BstAPI GCAn_nnn'nTGC
Cuts at: 0 206 2895 3144
Size: 206 2689 249
Fragments arranged by size:
2689 249 206
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1233 3144
Size: 1233 1911
BstYI r'GATC_y
Cuts at: 0 431 1089 1330 3144
Size: 431 658 241 1814
Fragments arranged by size:
1814 658 431 241
BtgI C'CryG_G
Cuts at: 0 1062 1079 1298 2069 2671 3144
Size: 1062 17 219 771 602 473
Fragments arranged by size:
1062 771 602 473 219 17
BtsI GCAGTG_nn'
Cuts at: 0 1125 3144
Size: 1125 2019
DraI TTT'AAA
Cuts at: 0 2060 3144
Size: 2060 1084
EaeI y'GGCC_r
Cuts at: 0 620 3144
Size: 620 2524
EagI C'GGCC_G
Cuts at: 0 620 3144
Size: 620 2524
EarI CTCTTCn'nnn_
Cuts at: 0 2084 2594 2682 2805 3144
Size: 2084 510 88 123 339
Fragments arranged by size:
2084 510 339 123 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1948 3144
Size: 1948 1196
EcoICRI GAG'CTC
Cuts at: 0 2921 3144
Size: 2921 223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1164 1343 1613 1646 2212 2399 2473 2779
Size: 1164 179 270 33 566 187 74 306
Cuts at: 2779 2907 3144
Size: 128 237
Fragments arranged by size:
1164 566 306 270 237 187 179 128
74 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2393 3144
Size: 2393 751
EcoO109I rG'GnC_Cy
Cuts at: 0 2403 3144
Size: 2403 741
FspI TGC'GCA
Cuts at: 0 1655 3144
Size: 1655 1489
HaeII r_GCGC'y
Cuts at: 0 1075 1769 2896 3144
Size: 1075 694 1127 248
Fragments arranged by size:
1127 1075 694 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 741 773 1245 1277 2145 2177
Size: 97 32 612 32 472 32 868 32
Cuts at: 2177 3144
Size: 967
Fragments arranged by size:
967 868 612 472 97 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2254 2787 3144
Size: 2254 533 357
Fragments arranged by size:
2254 533 357
HindIII A'AGCT_T
Cuts at: 0 1145 3144
Size: 1145 1999
KpnI G_GTAC'C
Cuts at: 0 737 3144
Size: 737 2407
MfeI C'AATT_G
Cuts at: 0 2085 3144
Size: 2085 1059
MslI CAynn'nnrTG
Cuts at: 0 1231 1481 1497 1811 2995 3144
Size: 1231 250 16 314 1184 149
Fragments arranged by size:
1231 1184 314 250 149 16
MspA1I CmG'CkG
Cuts at: 0 130 900 1167 1645 1819 2014 3144
Size: 130 770 267 478 174 195 1130
Fragments arranged by size:
1130 770 478 267 195 174 130
NaeI GCC'GGC
Cuts at: 0 1209 3144
Size: 1209 1935
NdeI CA'TA_TG
Cuts at: 0 2837 3144
Size: 2837 307
NgoMIV G'CCGG_C
Cuts at: 0 1207 3144
Size: 1207 1937
NsiI A_TGCA'T
Cuts at: 0 859 3144
Size: 859 2285
NspI r_CATG'y
Cuts at: 0 27 204 250 480 525 3144
Size: 27 177 46 230 45 2619
Fragments arranged by size:
2619 230 177 46 45 27
PciI A'CATG_T
Cuts at: 0 246 476 3144
Size: 246 230 2668
Fragments arranged by size:
2668 246 230
PflMI CCAn_nnn'nTGG
Cuts at: 0 1167 2190 3144
Size: 1167 1023 954
Fragments arranged by size:
1167 1023 954
PfoI T'CCnGG_A
Cuts at: 0 2682 3144
Size: 2682 462
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 333 365 3144
Size: 333 32 2779
Fragments arranged by size:
2779 333 32
PshAI GACnn'nnGTC
Cuts at: 0 2760 3144
Size: 2760 384
PspXI vC'TCGA_Gb
Cuts at: 0 780 3144
Size: 780 2364
PstI C_TGCA'G
Cuts at: 0 211 566 800 1524 2023 2727 3144
Size: 211 355 234 724 499 704 417
Fragments arranged by size:
724 704 499 417 355 234 211
PvuII CAG'CTG
Cuts at: 0 900 1167 1819 2014 3144
Size: 900 267 652 195 1130
Fragments arranged by size:
1130 900 652 267 195
SacI G_AGCT'C
Cuts at: 0 2923 3144
Size: 2923 221
SalI G'TCGA_C
Cuts at: 0 2252 3144
Size: 2252 892
SapI GCTCTTCn'nnn_
Cuts at: 0 2084 3144
Size: 2084 1060
SfcI C'TryA_G
Cuts at: 0 207 562 796 1520 1920 2019 2723 3144
Size: 207 355 234 724 400 99 704 421
Fragments arranged by size:
724 704 421 400 355 234 207 99
SmaI CCC'GGG
Cuts at: 0 2796 3144
Size: 2796 348
SmlI C'TyrA_G
Cuts at: 0 780 810 1726 3144
Size: 780 30 916 1418
Fragments arranged by size:
1418 916 780 30
SspI AAT'ATT
Cuts at: 0 1461 3144
Size: 1461 1683
StuI AGG'CCT
Cuts at: 0 1028 3045 3144
Size: 1028 2017 99
Fragments arranged by size:
2017 1028 99
StyI C'CwwG_G
Cuts at: 0 191 353 502 1928 2487 2908 3144
Size: 191 162 149 1426 559 421 236
Fragments arranged by size:
1426 559 421 236 191 162 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1981 3144
Size: 1981 1163
TatI w'GTAC_w
Cuts at: 0 47 3144
Size: 47 3097
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2276 2308 3144
Size: 2276 32 836
Fragments arranged by size:
2276 836 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1230 2835 3144
Size: 1230 1605 309
Fragments arranged by size:
1605 1230 309
XhoI C'TCGA_G
Cuts at: 0 780 3144
Size: 780 2364
XmaI C'CCGG_G
Cuts at: 0 2794 3144
Size: 2794 350
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AflIII AleI
AlfI AloI AlwNI ApaLI ApoI AvaI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BfrBI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrFI BsrGI BssHII BstAPI BstXI BstYI BtgI
BtsI DraI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I HincII HindIII KpnI
MfeI MslI MspA1I NaeI NdeI NgoMIV NsiI NspI
PciI PflMI PfoI PpiI PshAI PspXI PstI PvuII
SacI SalI SapI SfcI SmaI SmlI SspI StuI
StyI TaqII TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AclI AgeI AhdI ApaI AscI AseI AsiSI
AvrII BaeI BbeI BclI BglI BglII BlpI BmtI
BsaAI BsaBI BsaHI BsiWI BsmBI BspHI BsrBI BsrDI
BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraIII DrdI EcoRI EcoRV FalI FseI FspAI HpaI
KasI MluI MmeI MscI NarI NcoI NheI NotI
NruI PacI PasI PmeI PmlI PpuMI PsiI PspOMI
PsrI PvuI RsrII SacII SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SwaI Tth111I XbaI XmnI ZraI