Restriction map for
pF1KE3342
(Linear) (Six-base) MAPSORT of: KIEE3342.seq Check: 5852 from: 1 to: 2961
KIEE3342.orf
With 182 enzymes: SgfI *
March 2, 2011 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1858 2961
Size: 1858 1103
AatII G_ACGT'C
Cuts at: 0 1462 2961
Size: 1462 1499
AccI GT'mk_AC
Cuts at: 0 1766 2961
Size: 1766 1195
Acc65I G'GTAC_C
Cuts at: 0 1296 2186 2961
Size: 1296 890 775
Fragments arranged by size:
1296 890 775
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 1474 1510 1966 2961
Size: 109 1365 36 456 995
Fragments arranged by size:
1365 995 456 109 36
AflIII A'CryG_T
Cuts at: 0 254 2961
Size: 254 2707
AhdI GACnn_n'nnGTC
Cuts at: 0 491 2961
Size: 491 2470
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 574 1698 2961
Size: 574 1124 1263
Fragments arranged by size:
1263 1124 574
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 994 1026 1219 1251 2961
Size: 994 32 193 32 1710
Fragments arranged by size:
1710 994 193 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2791 2961
Size: 2791 170
ApaI G_GGCC'C
Cuts at: 0 1256 1550 2961
Size: 1256 294 1411
Fragments arranged by size:
1411 1256 294
ApaLI G'TGCA_C
Cuts at: 0 950 2961
Size: 950 2011
ApoI r'AATT_y
Cuts at: 0 1813 2961
Size: 1813 1148
AvaI C'yCGr_G
Cuts at: 0 198 283 302 635 944 1148 1370 2175
Size: 198 85 19 333 309 204 222 805
Cuts at: 2175 2663 2928 2961
Size: 488 265 33
Fragments arranged by size:
805 488 333 309 265 222 204 198
85 33 19
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1748 1781 2961
Size: 1748 33 1180
Fragments arranged by size:
1748 1180 33
BanI G'GyrC_C
Cuts at: 0 244 544 820 1296 1374 1423 2095 2186
Size: 244 300 276 476 78 49 672 91
Cuts at: 2186 2961
Size: 775
Fragments arranged by size:
775 672 476 300 276 244 91 78
49
BanII G_rGCy'C
Cuts at: 0 8 1166 1256 1550 2329 2961
Size: 8 1158 90 294 779 632
Fragments arranged by size:
1158 779 632 294 90 8
BbeI G_GCGC'C
Cuts at: 0 248 1427 2099 2961
Size: 248 1179 672 862
Fragments arranged by size:
1179 862 672 248
BbsI GAAGACnn'nnnn_
Cuts at: 0 330 1462 1796 2961
Size: 330 1132 334 1165
Fragments arranged by size:
1165 1132 334 330
BbvCI CC'TCA_GC
Cuts at: 0 785 2831 2961
Size: 785 2046 130
Fragments arranged by size:
2046 785 130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1617 1651 1703 1737 2961
Size: 1617 34 52 34 1224
Fragments arranged by size:
1617 1224 52 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1245 2961
Size: 1245 1716
BclI T'GATC_A
Cuts at: 0 2486 2961
Size: 2486 475
BglI GCCn_nnn'nGGC
Cuts at: 0 553 1004 2039 2368 2961
Size: 553 451 1035 329 593
Fragments arranged by size:
1035 593 553 451 329
BglII A'GATC_T
Cuts at: 0 2822 2961
Size: 2822 139
BlpI GC'TnA_GC
Cuts at: 0 505 2961
Size: 505 2456
Bme1580I G_kGCm'C
Cuts at: 0 198 653 816 823 933 954 1035 1256
Size: 198 455 163 7 110 21 81 221
Cuts at: 1256 1377 1550 1630 2961
Size: 121 173 80 1331
Fragments arranged by size:
1331 455 221 198 173 163 121 110
81 80 21 7
BmgBI CAC'GTC
Cuts at: 0 186 2484 2961
Size: 186 2298 477
Fragments arranged by size:
2298 477 186
BmrI ACTGGGnnnn_n'
Cuts at: 0 1373 2472 2961
Size: 1373 1099 489
Fragments arranged by size:
1373 1099 489
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1078 2319 2961
Size: 1078 1241 642
Fragments arranged by size:
1241 1078 642
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1060 2056 2314 2430 2961
Size: 1060 996 258 116 531
Fragments arranged by size:
1060 996 531 258 116
Bpu10I CC'TnA_GC
Cuts at: 0 785 1242 2831 2961
Size: 785 457 1589 130
Fragments arranged by size:
1589 785 457 130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1368 1885 2961
Size: 1368 517 1076
Fragments arranged by size:
1368 1076 517
BsaI GGTCTCn'nnnn_
Cuts at: 0 46 319 1118 2210 2961
Size: 46 273 799 1092 751
Fragments arranged by size:
1092 799 751 273 46
BsaAI yAC'GTr
Cuts at: 0 255 2961
Size: 255 2706
BsaBI GATnn'nnATC
Cuts at: 0 2347 2961
Size: 2347 614
BsaHI Gr'CG_yC
Cuts at: 0 245 1424 1459 2096 2961
Size: 245 1179 35 637 865
Fragments arranged by size:
1179 865 637 245 35
BsaWI w'CCGG_w
Cuts at: 0 2381 2579 2633 2961
Size: 2381 198 54 328
Fragments arranged by size:
2381 328 198 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 951 981 994 1024 2311 2341 2425 2443
Size: 951 30 13 30 1287 30 84 18
Cuts at: 2443 2455 2473 2675 2705 2961
Size: 12 18 202 30 256
Fragments arranged by size:
1287 951 256 202 84 30 30 30
30 18 18 13 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 999 2311 2961
Size: 999 1312 650
Fragments arranged by size:
1312 999 650
BseYI C'CCAG_C
Cuts at: 0 23 307 593 697 703 864 1339 1435
Size: 23 284 286 104 6 161 475 96
Cuts at: 1435 2961
Size: 1526
Fragments arranged by size:
1526 475 286 284 161 104 96 23
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 553 942 1000 1534 1886 2961
Size: 208 345 389 58 534 352 1075
Fragments arranged by size:
1075 534 389 352 345 208 58
BsiHKAI G_wGCw'C
Cuts at: 0 8 768 954 2018 2329 2961
Size: 8 760 186 1064 311 632
Fragments arranged by size:
1064 760 632 311 186 8
BsmI GAATG_Cn'
Cuts at: 0 2589 2961
Size: 2589 372
Bsp1286I G_dGCh'C
Cuts at: 0 8 198 653 768 816 823 933 954
Size: 8 190 455 115 48 7 110 21
Cuts at: 954 1035 1166 1256 1377 1550 1630 2018 2329
Size: 81 131 90 121 173 80 388 311
Cuts at: 2329 2961
Size: 632
Fragments arranged by size:
632 455 388 311 190 173 131 121
115 110 90 81 80 48 21 8
7
BspEI T'CCGG_A
Cuts at: 0 2381 2633 2961
Size: 2381 252 328
Fragments arranged by size:
2381 328 252
BspHI T'CATG_A
Cuts at: 0 2066 2961
Size: 2066 895
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1506 1858 2846 2961
Size: 1506 352 988 115
Fragments arranged by size:
1506 988 352 115
BsrBI CCG'CTC
Cuts at: 0 137 504 1965 2961
Size: 137 367 1461 996
Fragments arranged by size:
1461 996 367 137
BsrDI GCAATG_nn'
Cuts at: 0 1707 2372 2961
Size: 1707 665 589
Fragments arranged by size:
1707 665 589
BsrFI r'CCGG_y
Cuts at: 0 658 744 1537 2510 2946 2961
Size: 658 86 793 973 436 15
Fragments arranged by size:
973 793 658 436 86 15
BssHII G'CGCG_C
Cuts at: 0 1525 2961
Size: 1525 1436
BssSI C'ACGA_G
Cuts at: 0 2149 2322 2961
Size: 2149 173 639
Fragments arranged by size:
2149 639 173
BstAPI GCAn_nnn'nTGC
Cuts at: 0 162 2961
Size: 162 2799
BstEII G'GTnAC_C
Cuts at: 0 2046 2961
Size: 2046 915
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1509 2760 2961
Size: 1509 1251 201
Fragments arranged by size:
1509 1251 201
BstYI r'GATC_y
Cuts at: 0 2822 2961
Size: 2822 139
Bsu36I CC'TnA_GG
Cuts at: 0 107 1687 2961
Size: 107 1580 1274
Fragments arranged by size:
1580 1274 107
BtgI C'CryG_G
Cuts at: 0 235 368 425 653 979 1247 2961
Size: 235 133 57 228 326 268 1714
Fragments arranged by size:
1714 326 268 235 228 133 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 368 2154 2961
Size: 368 1786 807
Fragments arranged by size:
1786 807 368
BtsI GCAGTG_nn'
Cuts at: 0 127 1217 1313 2961
Size: 127 1090 96 1648
Fragments arranged by size:
1648 1090 127 96
ClaI AT'CG_AT
Cuts at: 0 1829 2961
Size: 1829 1132
DraIII CAC_nnn'GTG
Cuts at: 0 162 800 1381 2961
Size: 162 638 581 1580
Fragments arranged by size:
1580 638 581 162
DrdI GACnn_nn'nnGTC
Cuts at: 0 1402 2961
Size: 1402 1559
EaeI y'GGCC_r
Cuts at: 0 35 365 422 656 726 1050 1554 2885
Size: 35 330 57 234 70 324 504 1331
Cuts at: 2885 2961
Size: 76
Fragments arranged by size:
1331 504 330 324 234 76 70 57
35
EarI CTCTTCn'nnn_
Cuts at: 0 44 580 1847 2310 2961
Size: 44 536 1267 463 651
Fragments arranged by size:
1267 651 536 463 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2020 2385 2961
Size: 2020 365 576
Fragments arranged by size:
2020 576 365
EcoICRI GAG'CTC
Cuts at: 0 6 2327 2961
Size: 6 2321 634
Fragments arranged by size:
2321 634 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 1060 1474 1510 1966 2056 2314 2430
Size: 109 951 414 36 456 90 258 116
Cuts at: 2430 2961
Size: 531
Fragments arranged by size:
951 531 456 414 258 116 109 90
36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 145 1513 2961
Size: 145 1368 1448
Fragments arranged by size:
1448 1368 145
EcoO109I rG'GnC_Cy
Cuts at: 0 231 848 1059 1152 1252 1432 1547 1905
Size: 231 617 211 93 100 180 115 358
Cuts at: 1905 2678 2835 2961
Size: 773 157 126
Fragments arranged by size:
773 617 358 231 211 180 157 126
115 100 93
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 345 534 2898 2961
Size: 345 189 2364 63
Fragments arranged by size:
2364 345 189 63
FspI TGC'GCA
Cuts at: 0 166 2961
Size: 166 2795
FspAI rTGC'GCAy
Cuts at: 0 166 2961
Size: 166 2795
HaeII r_GCGC'y
Cuts at: 0 248 315 450 1427 2099 2618 2961
Size: 248 67 135 977 672 519 343
Fragments arranged by size:
977 672 519 343 248 135 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 90 122 277 309 477 509 618 650
Size: 90 32 155 32 168 32 109 32
Cuts at: 650 884 916 1727 1759 2425 2457 2961
Size: 234 32 811 32 666 32 504
Fragments arranged by size:
811 666 504 234 168 155 109 90
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 2244 2961
Size: 2244 717
KasI G'GCGC_C
Cuts at: 0 244 1423 2095 2961
Size: 244 1179 672 866
Fragments arranged by size:
1179 866 672 244
KpnI G_GTAC'C
Cuts at: 0 1300 2190 2961
Size: 1300 890 771
Fragments arranged by size:
1300 890 771
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1337 2961
Size: 1337 1624
MscI TGG'CCA
Cuts at: 0 728 2887 2961
Size: 728 2159 74
Fragments arranged by size:
2159 728 74
MslI CAynn'nnrTG
Cuts at: 0 409 1321 2758 2961
Size: 409 912 1437 203
Fragments arranged by size:
1437 912 409 203
MspA1I CmG'CkG
Cuts at: 0 427 597 761 784 981 1079 1611 1971
Size: 427 170 164 23 197 98 532 360
Cuts at: 1971 2703 2791 2961
Size: 732 88 170
Fragments arranged by size:
732 532 427 360 197 170 170 164
98 88 23
NaeI GCC'GGC
Cuts at: 0 1539 2961
Size: 1539 1422
NarI GG'CG_CC
Cuts at: 0 245 1424 2096 2961
Size: 245 1179 672 865
Fragments arranged by size:
1179 865 672 245
NgoMIV G'CCGG_C
Cuts at: 0 1537 2961
Size: 1537 1424
NspI r_CATG'y
Cuts at: 0 2061 2160 2184 2961
Size: 2061 99 24 777
Fragments arranged by size:
2061 777 99 24
PasI CC'CwG_GG
Cuts at: 0 451 816 1166 1909 2331 2961
Size: 451 365 350 743 422 630
Fragments arranged by size:
743 630 451 422 365 350
PflMI CCAn_nnn'nTGG
Cuts at: 0 2151 2354 2961
Size: 2151 203 607
Fragments arranged by size:
2151 607 203
PfoI T'CCnGG_A
Cuts at: 0 533 620 2291 2693 2961
Size: 533 87 1671 402 268
Fragments arranged by size:
1671 533 402 268 87
PmlI CAC'GTG
Cuts at: 0 255 2961
Size: 255 2706
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 994 1026 2961
Size: 994 32 1935
Fragments arranged by size:
1935 994 32
PpuMI rG'GwC_Cy
Cuts at: 0 231 848 1059 1152 1432 2835 2961
Size: 231 617 211 93 280 1403 126
Fragments arranged by size:
1403 617 280 231 211 126 93
PspOMI G'GGCC_C
Cuts at: 0 1252 1546 2961
Size: 1252 294 1415
Fragments arranged by size:
1415 1252 294
PspXI vC'TCGA_Gb
Cuts at: 0 283 2961
Size: 283 2678
PstI C_TGCA'G
Cuts at: 0 760 1308 2961
Size: 760 548 1653
Fragments arranged by size:
1653 760 548
PvuII CAG'CTG
Cuts at: 0 597 761 784 1611 2961
Size: 597 164 23 827 1350
Fragments arranged by size:
1350 827 597 164 23
SacI G_AGCT'C
Cuts at: 0 8 2329 2961
Size: 8 2321 632
Fragments arranged by size:
2321 632 8
SacII CC_GC'GG
Cuts at: 0 428 982 2961
Size: 428 554 1979
Fragments arranged by size:
1979 554 428
SanDI GG'GwC_CC
Cuts at: 0 231 1432 2961
Size: 231 1201 1529
Fragments arranged by size:
1529 1201 231
SexAI A'CCwGG_T
Cuts at: 0 1154 1508 2961
Size: 1154 354 1453
Fragments arranged by size:
1453 1154 354
SfcI C'TryA_G
Cuts at: 0 756 1304 2961
Size: 756 548 1657
Fragments arranged by size:
1657 756 548
SfoI GGC'GCC
Cuts at: 0 246 1425 2097 2961
Size: 246 1179 672 864
Fragments arranged by size:
1179 864 672 246
SmaI CCC'GGG
Cuts at: 0 200 946 1150 1372 2665 2930 2961
Size: 200 746 204 222 1293 265 31
Fragments arranged by size:
1293 746 265 222 204 200 31
SmlI C'TyrA_G
Cuts at: 0 283 1347 1900 2961
Size: 283 1064 553 1061
Fragments arranged by size:
1064 1061 553 283
SphI G_CATG'C
Cuts at: 0 2061 2160 2184 2961
Size: 2061 99 24 777
Fragments arranged by size:
2061 777 99 24
SspI AAT'ATT
Cuts at: 0 1720 2961
Size: 1720 1241
StuI AGG'CCT
Cuts at: 0 1993 2961
Size: 1993 968
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 84 1158 1304 1353 1392 1630 2961
Size: 84 1074 146 49 39 238 1331
Fragments arranged by size:
1331 1074 238 146 84 49 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 106 138 715 747 951 983 2675 2707
Size: 106 32 577 32 204 32 1692 32
Cuts at: 2707 2890 2922 2961
Size: 183 32 39
Fragments arranged by size:
1692 577 204 183 106 39 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1329 2961
Size: 1329 1632
XhoI C'TCGA_G
Cuts at: 0 283 2961
Size: 283 2678
XmaI C'CCGG_G
Cuts at: 0 198 944 1148 1370 2663 2928 2961
Size: 198 746 204 222 1293 265 33
Fragments arranged by size:
1293 746 265 222 204 198 33
ZraI GAC'GTC
Cuts at: 0 1460 2961
Size: 1460 1501
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BaeI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI PstI
PvuII SacI SacII SanDI SexAI SfcI SfoI SmaI
SmlI SphI SspI StuI TaqII TstI Tth111I XhoI
XmaI ZraI
Enzymes that do not cut:
AclI AfeI AflII AgeI AleI AscI AseI AsiSI
AvrII BamHI BfrBI BmtI BsiEI BsiWI BsmBI BsrGI
BstBI BstZ17I CspCI DraI EagI EcoRI EcoRV FseI
HindIII HpaI MfeI MluI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PshAI PsiI PsrI
PvuI RsrII SalI SapI SbfI ScaI SfiI SgfI
SgrAI SnaBI SpeI SrfI StyI SwaI TatI XbaI
XcmI XmnI