Restriction map for
pF1KE3341
(Linear) (Six-base) MAPSORT of: KIEE3341.seq Check: 4741 from: 1 to: 2958
KIEE3341.orf
With 182 enzymes: SgfI *
August 18, 2011 15:44 ..
AccI GT'mk_AC
Cuts at: 0 74 2958
Size: 74 2884
Acc65I G'GTAC_C
Cuts at: 0 762 2958
Size: 762 2196
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1554 2958
Size: 1554 1404
AflIII A'CryG_T
Cuts at: 0 2627 2958
Size: 2627 331
AgeI A'CCGG_T
Cuts at: 0 2777 2958
Size: 2777 181
AleI CACnn'nnGTG
Cuts at: 0 466 2958
Size: 466 2492
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1687 2958
Size: 1687 1271
AlwNI CAG_nnn'CTG
Cuts at: 0 661 1291 1555 2803 2958
Size: 661 630 264 1248 155
Fragments arranged by size:
1248 661 630 264 155
ApaLI G'TGCA_C
Cuts at: 0 918 2830 2958
Size: 918 1912 128
Fragments arranged by size:
1912 918 128
ApoI r'AATT_y
Cuts at: 0 304 1891 2398 2608 2725 2958
Size: 304 1587 507 210 117 233
Fragments arranged by size:
1587 507 304 233 210 117
AvaI C'yCGr_G
Cuts at: 0 269 1659 2303 2958
Size: 269 1390 644 655
Fragments arranged by size:
1390 655 644 269
BamHI G'GATC_C
Cuts at: 0 2709 2958
Size: 2709 249
BanI G'GyrC_C
Cuts at: 0 762 2958
Size: 762 2196
BanII G_rGCy'C
Cuts at: 0 282 1580 2686 2958
Size: 282 1298 1106 272
Fragments arranged by size:
1298 1106 282 272
BbsI GAAGACnn'nnnn_
Cuts at: 0 322 662 1186 2958
Size: 322 340 524 1772
Fragments arranged by size:
1772 524 340 322
BclI T'GATC_A
Cuts at: 0 2087 2958
Size: 2087 871
BfrBI ATG'CAT
Cuts at: 0 2125 2958
Size: 2125 833
BglII A'GATC_T
Cuts at: 0 109 1940 2958
Size: 109 1831 1018
Fragments arranged by size:
1831 1018 109
Bme1580I G_kGCm'C
Cuts at: 0 880 922 2834 2958
Size: 880 42 1912 124
Fragments arranged by size:
1912 880 124 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 630 2958
Size: 630 2328
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1067 2130 2958
Size: 1067 1063 828
Fragments arranged by size:
1067 1063 828
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2670 2743 2958
Size: 2670 73 215
Fragments arranged by size:
2670 215 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 333 2332 2958
Size: 333 1999 626
Fragments arranged by size:
1999 626 333
BsaI GGTCTCn'nnnn_
Cuts at: 0 1656 2958
Size: 1656 1302
BsaAI yAC'GTr
Cuts at: 0 1536 1802 2835 2958
Size: 1536 266 1033 123
Fragments arranged by size:
1536 1033 266 123
BsaBI GATnn'nnATC
Cuts at: 0 2359 2958
Size: 2359 599
BsaHI Gr'CG_yC
Cuts at: 0 338 1914 2958
Size: 338 1576 1044
Fragments arranged by size:
1576 1044 338
BsaWI w'CCGG_w
Cuts at: 0 2322 2363 2777 2958
Size: 2322 41 414 181
Fragments arranged by size:
2322 414 181 41
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1704 1734 2958
Size: 1704 30 1224
Fragments arranged by size:
1704 1224 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 814 2004 2607 2958
Size: 814 1190 603 351
Fragments arranged by size:
1190 814 603 351
BseYI C'CCAG_C
Cuts at: 0 4 232 790 1120 2958
Size: 4 228 558 330 1838
Fragments arranged by size:
1838 558 330 228 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 957 2958
Size: 957 2001
BsiHKAI G_wGCw'C
Cuts at: 0 922 2686 2834 2958
Size: 922 1764 148 124
Fragments arranged by size:
1764 922 148 124
BsiWI C'GTAC_G
Cuts at: 0 1802 2456 2958
Size: 1802 654 502
Fragments arranged by size:
1802 654 502
BsmI GAATG_Cn'
Cuts at: 0 820 2125 2958
Size: 820 1305 833
Fragments arranged by size:
1305 833 820
BsmBI CGTCTCn'nnnn_
Cuts at: 0 352 1707 1921 2958
Size: 352 1355 214 1037
Fragments arranged by size:
1355 1037 352 214
Bsp1286I G_dGCh'C
Cuts at: 0 282 880 922 1580 2686 2834 2958
Size: 282 598 42 658 1106 148 124
Fragments arranged by size:
1106 658 598 282 148 124 42
BspEI T'CCGG_A
Cuts at: 0 2322 2958
Size: 2322 636
BspHI T'CATG_A
Cuts at: 0 488 2958
Size: 488 2470
BsrBI CCG'CTC
Cuts at: 0 804 809 2958
Size: 804 5 2149
Fragments arranged by size:
2149 804 5
BsrDI GCAATG_nn'
Cuts at: 0 225 440 1328 1688 2549 2918 2958
Size: 225 215 888 360 861 369 40
Fragments arranged by size:
888 861 369 360 225 215 40
BsrFI r'CCGG_y
Cuts at: 0 2777 2958
Size: 2777 181
BssSI C'ACGA_G
Cuts at: 0 915 2958
Size: 915 2043
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2934 2958
Size: 2934 24
BstEII G'GTnAC_C
Cuts at: 0 1114 2525 2958
Size: 1114 1411 433
Fragments arranged by size:
1411 1114 433
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1335 2019 2772 2958
Size: 1335 684 753 186
Fragments arranged by size:
1335 753 684 186
BstYI r'GATC_y
Cuts at: 0 109 506 1809 1940 2709 2958
Size: 109 397 1303 131 769 249
Fragments arranged by size:
1303 769 397 249 131 109
BstZ17I GTA'TAC
Cuts at: 0 75 2958
Size: 75 2883
Bsu36I CC'TnA_GG
Cuts at: 0 2131 2958
Size: 2131 827
BtgI C'CryG_G
Cuts at: 0 854 2958
Size: 854 2104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 558 2958
Size: 558 2400
BtsI GCAGTG_nn'
Cuts at: 0 1671 1829 1914 2696 2907 2958
Size: 1671 158 85 782 211 51
Fragments arranged by size:
1671 782 211 158 85 51
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1086 1121 2958
Size: 1086 35 1837
Fragments arranged by size:
1837 1086 35
DraIII CAC_nnn'GTG
Cuts at: 0 917 2958
Size: 917 2041
EaeI y'GGCC_r
Cuts at: 0 1065 2240 2958
Size: 1065 1175 718
Fragments arranged by size:
1175 1065 718
EarI CTCTTCn'nnn_
Cuts at: 0 43 707 1748 2659 2958
Size: 43 664 1041 911 299
Fragments arranged by size:
1041 911 664 299 43
EcoICRI GAG'CTC
Cuts at: 0 2684 2958
Size: 2684 274
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1554 2670 2743 2958
Size: 1554 1116 73 215
Fragments arranged by size:
1554 1116 215 73
EcoNI CCTnn'n_nnAGG
Cuts at: 0 149 2005 2958
Size: 149 1856 953
Fragments arranged by size:
1856 953 149
EcoO109I rG'GnC_Cy
Cuts at: 0 566 2468 2847 2958
Size: 566 1902 379 111
Fragments arranged by size:
1902 566 379 111
EcoRI G'AATT_C
Cuts at: 0 2725 2958
Size: 2725 233
EcoRV GAT'ATC
Cuts at: 0 1497 2958
Size: 1497 1461
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2808 2958
Size: 2808 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 94 126 946 978 2141 2173 2701 2733
Size: 94 32 820 32 1163 32 528 32
Cuts at: 2733 2958
Size: 225
Fragments arranged by size:
1163 820 528 225 94 32 32 32
32
HincII GTy'rAC
Cuts at: 0 702 1017 2625 2958
Size: 702 315 1608 333
Fragments arranged by size:
1608 702 333 315
HindIII A'AGCT_T
Cuts at: 0 821 2958
Size: 821 2137
KpnI G_GTAC'C
Cuts at: 0 766 2958
Size: 766 2192
MfeI C'AATT_G
Cuts at: 0 2384 2958
Size: 2384 574
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2612 2687 2958
Size: 2612 75 271
Fragments arranged by size:
2612 271 75
MslI CAynn'nnrTG
Cuts at: 0 466 2257 2770 2958
Size: 466 1791 513 188
Fragments arranged by size:
1791 513 466 188
MspA1I CmG'CkG
Cuts at: 0 1480 1555 2166 2565 2800 2958
Size: 1480 75 611 399 235 158
Fragments arranged by size:
1480 611 399 235 158 75
NdeI CA'TA_TG
Cuts at: 0 1785 2958
Size: 1785 1173
NsiI A_TGCA'T
Cuts at: 0 2127 2958
Size: 2127 831
NspI r_CATG'y
Cuts at: 0 2178 2301 2631 2958
Size: 2178 123 330 327
Fragments arranged by size:
2178 330 327 123
PciI A'CATG_T
Cuts at: 0 2627 2958
Size: 2627 331
PfoI T'CCnGG_A
Cuts at: 0 509 1412 1609 2958
Size: 509 903 197 1349
Fragments arranged by size:
1349 903 509 197
PmlI CAC'GTG
Cuts at: 0 1536 2835 2958
Size: 1536 1299 123
Fragments arranged by size:
1536 1299 123
PpuMI rG'GwC_Cy
Cuts at: 0 2847 2958
Size: 2847 111
PvuII CAG'CTG
Cuts at: 0 1480 1555 2166 2565 2800 2958
Size: 1480 75 611 399 235 158
Fragments arranged by size:
1480 611 399 235 158 75
SacI G_AGCT'C
Cuts at: 0 2686 2958
Size: 2686 272
SmlI C'TyrA_G
Cuts at: 0 312 2311 2958
Size: 312 1999 647
Fragments arranged by size:
1999 647 312
SnaBI TAC'GTA
Cuts at: 0 1802 2958
Size: 1802 1156
SphI G_CATG'C
Cuts at: 0 2178 2958
Size: 2178 780
StuI AGG'CCT
Cuts at: 0 1505 2604 2958
Size: 1505 1099 354
Fragments arranged by size:
1505 1099 354
StyI C'CwwG_G
Cuts at: 0 1580 2117 2958
Size: 1580 537 841
Fragments arranged by size:
1580 841 537
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 119 478 944 2958
Size: 119 359 466 2014
Fragments arranged by size:
2014 466 359 119
TatI w'GTAC_w
Cuts at: 0 371 734 2099 2958
Size: 371 363 1365 859
Fragments arranged by size:
1365 859 371 363
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 142 174 605 637 2237 2269 2958
Size: 142 32 431 32 1600 32 689
Fragments arranged by size:
1600 689 431 142 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 55 2958
Size: 55 2903
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 472 1644 2958
Size: 472 1172 1314
Fragments arranged by size:
1314 1172 472
Enzymes that do cut:
AccI Acc65I AcuI AflIII AgeI AleI AlfI AlwNI
ApaLI ApoI AvaI BamHI BanI BanII BbsI BclI
BfrBI BglII Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI
BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I
HincII HindIII KpnI MfeI MmeI MslI MspA1I NdeI
NsiI NspI PciI PfoI PmlI PpuMI PvuII SacI
SmlI SnaBI SphI StuI StyI TaqII TatI TstI
Tth111I XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AhdI AloI ApaI
AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI
BciVI BglI BlpI BmgBI BmtI Bpu10I BsiEI BspMI
BsrGI BssHII BstBI ClaI DraI DrdI EagI EciI
FseI FspI FspAI HaeII HpaI KasI MluI MscI
NaeI NarI NcoI NgoMIV NheI NotI NruI PacI
PasI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SpeI SrfI SspI SwaI XbaI XhoI XmaI
XmnI ZraI