Restriction map for
pF1KE3319
(Linear) (Six-base) MAPSORT of: KIEE3319.seq Check: 22 from: 1 to: 2667
KIEE3319.orf
With 182 enzymes: SgfI *
March 2, 2011 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1032 2401 2592 2667
Size: 1032 1369 191 75
Fragments arranged by size:
1369 1032 191 75
Acc65I G'GTAC_C
Cuts at: 0 1209 2667
Size: 1209 1458
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 373 481 785 1651 1789 2074 2113
Size: 112 261 108 304 866 138 285 39
Cuts at: 2113 2436 2667
Size: 323 231
Fragments arranged by size:
866 323 304 285 261 231 138 112
108 39
AflIII A'CryG_T
Cuts at: 0 386 2667
Size: 386 2281
AhdI GACnn_n'nnGTC
Cuts at: 0 2643 2667
Size: 2643 24
AleI CACnn'nnGTG
Cuts at: 0 959 2667
Size: 959 1708
AlwNI CAG_nnn'CTG
Cuts at: 0 165 168 444 526 684 717 873 1221
Size: 165 3 276 82 158 33 156 348
Cuts at: 1221 2667
Size: 1446
Fragments arranged by size:
1446 348 276 165 158 156 82 33
3
AvaI C'yCGr_G
Cuts at: 0 761 1401 1969 2182 2404 2667
Size: 761 640 568 213 222 263
Fragments arranged by size:
761 640 568 263 222 213
AvrII C'CTAG_G
Cuts at: 0 1609 2667
Size: 1609 1058
BamHI G'GATC_C
Cuts at: 0 1826 2667
Size: 1826 841
BanI G'GyrC_C
Cuts at: 0 12 314 400 586 780 1209 1364 1853
Size: 12 302 86 186 194 429 155 489
Cuts at: 1853 2161 2667
Size: 308 506
Fragments arranged by size:
506 489 429 308 302 194 186 155
86 12
BanII G_rGCy'C
Cuts at: 0 312 1095 1976 2667
Size: 312 783 881 691
Fragments arranged by size:
881 783 691 312
BbeI G_GCGC'C
Cuts at: 0 16 590 1368 2667
Size: 16 574 778 1299
Fragments arranged by size:
1299 778 574 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 1504 1956 2667
Size: 1504 452 711
Fragments arranged by size:
1504 711 452
BbvCI CC'TCA_GC
Cuts at: 0 581 647 1201 2667
Size: 581 66 554 1466
Fragments arranged by size:
1466 581 554 66
BciVI GTATCCnnnnn_n'
Cuts at: 0 781 2667
Size: 781 1886
BglI GCCn_nnn'nGGC
Cuts at: 0 149 857 910 2667
Size: 149 708 53 1757
Fragments arranged by size:
1757 708 149 53
BlpI GC'TnA_GC
Cuts at: 0 263 1683 1786 2667
Size: 263 1420 103 881
Fragments arranged by size:
1420 881 263 103
Bme1580I G_kGCm'C
Cuts at: 0 1856 2272 2392 2667
Size: 1856 416 120 275
Fragments arranged by size:
1856 416 275 120
BmgBI CAC'GTC
Cuts at: 0 1621 2667
Size: 1621 1046
BmrI ACTGGGnnnn_n'
Cuts at: 0 1472 2667
Size: 1472 1195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 517 739 1105 1123 1852 1878 2365
Size: 211 306 222 366 18 729 26 487
Cuts at: 2365 2667
Size: 302
Fragments arranged by size:
729 487 366 306 302 222 211 26
18
Bpu10I CC'TnA_GC
Cuts at: 0 304 527 581 647 1201 2667
Size: 304 223 54 66 554 1466
Fragments arranged by size:
1466 554 304 223 66 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1183 2380 2667
Size: 1183 1197 287
Fragments arranged by size:
1197 1183 287
BsaAI yAC'GTr
Cuts at: 0 389 1992 2667
Size: 389 1603 675
Fragments arranged by size:
1603 675 389
BsaBI GATnn'nnATC
Cuts at: 0 484 1948 2667
Size: 484 1464 719
Fragments arranged by size:
1464 719 484
BsaHI Gr'CG_yC
Cuts at: 0 13 587 1365 2324 2667
Size: 13 574 778 959 343
Fragments arranged by size:
959 778 574 343 13
BsaWI w'CCGG_w
Cuts at: 0 329 2141 2667
Size: 329 1812 526
Fragments arranged by size:
1812 526 329
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 674 704 2667
Size: 674 30 1963
Fragments arranged by size:
1963 674 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 556 1588 2368 2443 2590 2667
Size: 19 537 1032 780 75 147 77
Fragments arranged by size:
1032 780 537 147 77 75 19
BseYI C'CCAG_C
Cuts at: 0 258 434 659 794 897 1034 1692 2232
Size: 258 176 225 135 103 137 658 540
Cuts at: 2232 2667
Size: 435
Fragments arranged by size:
658 540 435 258 225 176 137 135
103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 205 544 1830 2570 2667
Size: 178 27 339 1286 740 97
Fragments arranged by size:
1286 740 339 178 97 27
BsiEI CG_ry'CG
Cuts at: 0 289 905 2667
Size: 289 616 1762
Fragments arranged by size:
1762 616 289
BsiHKAI G_wGCw'C
Cuts at: 0 1408 1787 2667
Size: 1408 379 880
Fragments arranged by size:
1408 880 379
Bsp1286I G_dGCh'C
Cuts at: 0 312 1095 1408 1787 1856 1976 2272 2392
Size: 312 783 313 379 69 120 296 120
Cuts at: 2392 2667
Size: 275
Fragments arranged by size:
783 379 313 312 296 275 120 120
69
BspEI T'CCGG_A
Cuts at: 0 329 2667
Size: 329 2338
BspHI T'CATG_A
Cuts at: 0 1373 1940 2667
Size: 1373 567 727
Fragments arranged by size:
1373 727 567
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 125 834 1032 1388 2401 2540 2592 2667
Size: 125 709 198 356 1013 139 52 75
Fragments arranged by size:
1013 709 356 198 139 125 75 52
BssSI C'ACGA_G
Cuts at: 0 1954 2026 2667
Size: 1954 72 641
Fragments arranged by size:
1954 641 72
BstAPI GCAn_nnn'nTGC
Cuts at: 0 165 168 444 558 1221 1782 2475 2667
Size: 165 3 276 114 663 561 693 192
Fragments arranged by size:
693 663 561 276 192 165 114 3
BstEII G'GTnAC_C
Cuts at: 0 1236 1933 2667
Size: 1236 697 734
Fragments arranged by size:
1236 734 697
BstXI CCAn_nnnn'nTGG
Cuts at: 0 597 2667
Size: 597 2070
BstYI r'GATC_y
Cuts at: 0 1826 1949 2667
Size: 1826 123 718
Fragments arranged by size:
1826 718 123
Bsu36I CC'TnA_GG
Cuts at: 0 276 1324 2667
Size: 276 1048 1343
Fragments arranged by size:
1343 1048 276
BtgI C'CryG_G
Cuts at: 0 16 289 2667
Size: 16 273 2378
Fragments arranged by size:
2378 273 16
BtsI GCAGTG_nn'
Cuts at: 0 838 1447 2603 2667
Size: 838 609 1156 64
Fragments arranged by size:
1156 838 609 64
DrdI GACnn_nn'nnGTC
Cuts at: 0 2311 2667
Size: 2311 356
EaeI y'GGCC_r
Cuts at: 0 128 286 902 911 963 1046 2667
Size: 128 158 616 9 52 83 1621
Fragments arranged by size:
1621 616 158 128 83 52 9
EagI C'GGCC_G
Cuts at: 0 286 902 2667
Size: 286 616 1765
Fragments arranged by size:
1765 616 286
EarI CTCTTCn'nnn_
Cuts at: 0 1508 2288 2667
Size: 1508 780 379
Fragments arranged by size:
1508 780 379
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 58 553 838 2667
Size: 58 495 285 1829
Fragments arranged by size:
1829 495 285 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 211 373 481 517 739 785 1105
Size: 112 99 162 108 36 222 46 320
Cuts at: 1105 1123 1651 1789 1852 1878 2074 2113 2365
Size: 18 528 138 63 26 196 39 252
Cuts at: 2365 2436 2667
Size: 71 231
Fragments arranged by size:
528 320 252 231 222 196 162 138
112 108 99 71 63 46 39 36
26 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1555 2667
Size: 1555 1112
EcoO109I rG'GnC_Cy
Cuts at: 0 280 509 523 1716 2049 2667
Size: 280 229 14 1193 333 618
Fragments arranged by size:
1193 618 333 280 229 14
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1521 2667
Size: 1521 1146
FspI TGC'GCA
Cuts at: 0 748 2667
Size: 748 1919
HaeII r_GCGC'y
Cuts at: 0 16 590 894 1368 2403 2667
Size: 16 574 304 474 1035 264
Fragments arranged by size:
1035 574 474 304 264 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1552 1584 2629 2661 2667
Size: 1552 32 1045 32 6
Fragments arranged by size:
1552 1045 32 32 6
HincII GTy'rAC
Cuts at: 0 2316 2667
Size: 2316 351
KasI G'GCGC_C
Cuts at: 0 12 586 1364 2667
Size: 12 574 778 1303
Fragments arranged by size:
1303 778 574 12
KpnI G_GTAC'C
Cuts at: 0 1213 2667
Size: 1213 1454
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 2039 2667
Size: 203 1836 628
Fragments arranged by size:
1836 628 203
MscI TGG'CCA
Cuts at: 0 130 913 2667
Size: 130 783 1754
Fragments arranged by size:
1754 783 130
MslI CAynn'nnrTG
Cuts at: 0 959 1958 2667
Size: 959 999 709
Fragments arranged by size:
999 959 709
MspA1I CmG'CkG
Cuts at: 0 18 165 198 262 339 430 459 574
Size: 18 147 33 64 77 91 29 115
Cuts at: 574 714 901 1679 2667
Size: 140 187 778 988
Fragments arranged by size:
988 778 187 147 140 115 91 77
64 33 29 18
NarI GG'CG_CC
Cuts at: 0 13 587 1365 2667
Size: 13 574 778 1302
Fragments arranged by size:
1302 778 574 13
NdeI CA'TA_TG
Cuts at: 0 1601 2667
Size: 1601 1066
NotI GC'GGCC_GC
Cuts at: 0 902 2667
Size: 902 1765
NspI r_CATG'y
Cuts at: 0 346 2667
Size: 346 2321
PasI CC'CwG_GG
Cuts at: 0 247 2082 2278 2667
Size: 247 1835 196 389
Fragments arranged by size:
1835 389 247 196
PflMI CCAn_nnn'nTGG
Cuts at: 0 2465 2667
Size: 2465 202
PfoI T'CCnGG_A
Cuts at: 0 1172 1829 2627 2657 2667
Size: 1172 657 798 30 10
Fragments arranged by size:
1172 798 657 30 10
PmlI CAC'GTG
Cuts at: 0 389 2667
Size: 389 2278
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2257 2289 2536 2568 2667
Size: 2257 32 247 32 99
Fragments arranged by size:
2257 247 99 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 280 509 1716 2049 2667
Size: 280 229 1207 333 618
Fragments arranged by size:
1207 618 333 280 229
PshAI GACnn'nnGTC
Cuts at: 0 1072 2667
Size: 1072 1595
PstI C_TGCA'G
Cuts at: 0 845 1280 1396 2018 2667
Size: 845 435 116 622 649
Fragments arranged by size:
845 649 622 435 116
PvuII CAG'CTG
Cuts at: 0 165 198 262 339 430 459 574 714
Size: 165 33 64 77 91 29 115 140
Cuts at: 714 2667
Size: 1953
Fragments arranged by size:
1953 165 140 115 91 77 64 33
29
SacII CC_GC'GG
Cuts at: 0 19 2667
Size: 19 2648
SanDI GG'GwC_CC
Cuts at: 0 509 2667
Size: 509 2158
SfcI C'TryA_G
Cuts at: 0 547 664 841 1276 1392 2014 2667
Size: 547 117 177 435 116 622 653
Fragments arranged by size:
653 622 547 435 177 117 116
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 910 2667
Size: 910 1757
SfoI GGC'GCC
Cuts at: 0 14 588 1366 2667
Size: 14 574 778 1301
Fragments arranged by size:
1301 778 574 14
SmlI C'TyrA_G
Cuts at: 0 1162 2359 2667
Size: 1162 1197 308
Fragments arranged by size:
1197 1162 308
StuI AGG'CCT
Cuts at: 0 1459 2667
Size: 1459 1208
StyI C'CwwG_G
Cuts at: 0 407 704 944 1505 1609 2667
Size: 407 297 240 561 104 1058
Fragments arranged by size:
1058 561 407 297 240 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1498 1545 2224 2251 2667
Size: 1498 47 679 27 416
Fragments arranged by size:
1498 679 416 47 27
TatI w'GTAC_w
Cuts at: 0 1811 2667
Size: 1811 856
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 830 862 1180 1212 1578 1605 1610 1637
Size: 830 32 318 32 366 27 5 27
Cuts at: 1637 2667
Size: 1030
Fragments arranged by size:
1030 830 366 318 32 32 27 27
5
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 186 987 2667
Size: 186 801 1680
Fragments arranged by size:
1680 801 186
Enzymes that do cut:
AarI Acc65I AcuI AflIII AhdI AleI AlwNI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI
BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I HincII KasI KpnI MmeI MscI MslI
MspA1I NarI NdeI NotI NspI PasI PflMI PfoI
PmlI PpiI PpuMI PshAI PstI PvuII SacII SanDI
SfcI SfiI SfoI SmlI StuI StyI TaqII TatI
TstI XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AlfI AloI
ApaI ApaLI ApoI AscI AseI AsiSI BaeI BcgI
BclI BfrBI BglII BmtI BplI BsaI BsiWI BsmI
BsmBI BsrBI BsrDI BsrFI BsrGI BssHII BstBI BstZ17I
BtgZI ClaI CspCI DraI DraIII EcoICRI EcoRI EcoRV
FseI FspAI HindIII HpaI MfeI MluI NaeI NcoI
NgoMIV NheI NruI NsiI PacI PciI PmeI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SalI SapI
SbfI ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI
XmnI ZraI