Restriction map for
pF1KE3281
(Linear) (Six-base) MAPSORT of: KIEE3281.seq Check: 4733 from: 1 to: 2136
KIEE3281.orf
With 182 enzymes: SgfI *
March 4, 2011 18:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 846 2136
Size: 846 1290
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1323 1507 1901 2136
Size: 1323 184 394 235
Fragments arranged by size:
1323 394 235 184
AgeI A'CCGG_T
Cuts at: 0 879 2136
Size: 879 1257
AleI CACnn'nnGTG
Cuts at: 0 2062 2136
Size: 2062 74
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1298 2136
Size: 1298 838
AlwNI CAG_nnn'CTG
Cuts at: 0 241 2136
Size: 241 1895
ApoI r'AATT_y
Cuts at: 0 255 285 645 980 1035 1464 2136
Size: 255 30 360 335 55 429 672
Fragments arranged by size:
672 429 360 335 255 55 30
AseI AT'TA_AT
Cuts at: 0 1258 1517 2136
Size: 1258 259 619
Fragments arranged by size:
1258 619 259
AvaI C'yCGr_G
Cuts at: 0 212 659 2136
Size: 212 447 1477
Fragments arranged by size:
1477 447 212
AvrII C'CTAG_G
Cuts at: 0 1129 2136
Size: 1129 1007
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 656 689 2136
Size: 656 33 1447
Fragments arranged by size:
1447 656 33
BamHI G'GATC_C
Cuts at: 0 432 2136
Size: 432 1704
BanI G'GyrC_C
Cuts at: 0 98 655 2136
Size: 98 557 1481
Fragments arranged by size:
1481 557 98
BanII G_rGCy'C
Cuts at: 0 185 213 381 630 842 1129 1830 2136
Size: 185 28 168 249 212 287 701 306
Fragments arranged by size:
701 306 287 249 212 185 168 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 31 1124 2136
Size: 31 1093 1012
Fragments arranged by size:
1093 1012 31
BbvCI CC'TCA_GC
Cuts at: 0 302 2136
Size: 302 1834
BciVI GTATCCnnnnn_n'
Cuts at: 0 869 1464 2136
Size: 869 595 672
Fragments arranged by size:
869 672 595
BclI T'GATC_A
Cuts at: 0 6 1047 2136
Size: 6 1041 1089
Fragments arranged by size:
1089 1041 6
BglI GCCn_nnn'nGGC
Cuts at: 0 193 2136
Size: 193 1943
BglII A'GATC_T
Cuts at: 0 81 323 1397 1962 2136
Size: 81 242 1074 565 174
Fragments arranged by size:
1074 565 242 174 81
BlpI GC'TnA_GC
Cuts at: 0 1917 2136
Size: 1917 219
Bme1580I G_kGCm'C
Cuts at: 0 159 660 2136
Size: 159 501 1476
Fragments arranged by size:
1476 501 159
BmgBI CAC'GTC
Cuts at: 0 2060 2136
Size: 2060 76
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 945 2136
Size: 945 1191
Bpu10I CC'TnA_GC
Cuts at: 0 302 2136
Size: 302 1834
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1143 1347 2136
Size: 1143 204 789
Fragments arranged by size:
1143 789 204
BsaI GGTCTCn'nnnn_
Cuts at: 0 961 2136
Size: 961 1175
BsaAI yAC'GTr
Cuts at: 0 1426 2136
Size: 1426 710
BsaWI w'CCGG_w
Cuts at: 0 879 2136
Size: 879 1257
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 869 899 2136
Size: 869 30 1237
Fragments arranged by size:
1237 869 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 877 910 1044 2136
Size: 877 33 134 1092
Fragments arranged by size:
1092 877 134 33
BseYI C'CCAG_C
Cuts at: 0 1662 2136
Size: 1662 474
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 854 2113 2136
Size: 854 1259 23
Fragments arranged by size:
1259 854 23
BsiHKAI G_wGCw'C
Cuts at: 0 213 1199 2136
Size: 213 986 937
Fragments arranged by size:
986 937 213
BsmI GAATG_Cn'
Cuts at: 0 1287 2136
Size: 1287 849
Bsp1286I G_dGCh'C
Cuts at: 0 159 185 213 381 630 660 842 1129
Size: 159 26 28 168 249 30 182 287
Cuts at: 1129 1199 1830 2136
Size: 70 631 306
Fragments arranged by size:
631 306 287 249 182 168 159 70
30 28 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 846 2136
Size: 846 1290
BsrDI GCAATG_nn'
Cuts at: 0 1643 2136
Size: 1643 493
BsrFI r'CCGG_y
Cuts at: 0 879 2136
Size: 879 1257
BsrGI T'GTAC_A
Cuts at: 0 1799 2136
Size: 1799 337
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1084 2136
Size: 1084 1052
BstEII G'GTnAC_C
Cuts at: 0 612 2136
Size: 612 1524
BstYI r'GATC_y
Cuts at: 0 81 323 432 1397 1962 2136
Size: 81 242 109 965 565 174
Fragments arranged by size:
965 565 242 174 109 81
DraI TTT'AAA
Cuts at: 0 1368 2136
Size: 1368 768
DraIII CAC_nnn'GTG
Cuts at: 0 543 1221 2136
Size: 543 678 915
Fragments arranged by size:
915 678 543
DrdI GACnn_nn'nnGTC
Cuts at: 0 553 2136
Size: 553 1583
EaeI y'GGCC_r
Cuts at: 0 2129 2136
Size: 2129 7
EarI CTCTTCn'nnn_
Cuts at: 0 524 1342 1928 1939 2136
Size: 524 818 586 11 197
Fragments arranged by size:
818 586 524 197 11
EcoICRI GAG'CTC
Cuts at: 0 211 2136
Size: 211 1925
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 945 1323 1507 1901 2136
Size: 945 378 184 394 235
Fragments arranged by size:
945 394 378 235 184
EcoO109I rG'GnC_Cy
Cuts at: 0 960 2136
Size: 960 1176
EcoRV GAT'ATC
Cuts at: 0 1431 2136
Size: 1431 705
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1601 1759 2136
Size: 1601 158 377
Fragments arranged by size:
1601 377 158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 392 424 596 628 1039 1071 2136
Size: 392 32 172 32 411 32 1065
Fragments arranged by size:
1065 411 392 172 32 32 32
HincII GTy'rAC
Cuts at: 0 558 2136
Size: 558 1578
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2049 2136
Size: 2049 87
MscI TGG'CCA
Cuts at: 0 2131 2136
Size: 2131 5
MslI CAynn'nnrTG
Cuts at: 0 782 1807 2062 2136
Size: 782 1025 255 74
Fragments arranged by size:
1025 782 255 74
MspA1I CmG'CkG
Cuts at: 0 811 1911 2136
Size: 811 1100 225
Fragments arranged by size:
1100 811 225
PflMI CCAn_nnn'nTGG
Cuts at: 0 462 1221 1669 2136
Size: 462 759 448 467
Fragments arranged by size:
759 467 462 448
PpuMI rG'GwC_Cy
Cuts at: 0 960 2136
Size: 960 1176
PstI C_TGCA'G
Cuts at: 0 591 1089 2136
Size: 591 498 1047
Fragments arranged by size:
1047 591 498
PvuII CAG'CTG
Cuts at: 0 1911 2136
Size: 1911 225
SacI G_AGCT'C
Cuts at: 0 213 2136
Size: 213 1923
SapI GCTCTTCn'nnn_
Cuts at: 0 524 1928 2136
Size: 524 1404 208
Fragments arranged by size:
1404 524 208
SexAI A'CCwGG_T
Cuts at: 0 1217 2136
Size: 1217 919
SfcI C'TryA_G
Cuts at: 0 587 1085 2136
Size: 587 498 1051
Fragments arranged by size:
1051 587 498
SmlI C'TyrA_G
Cuts at: 0 1122 1326 2136
Size: 1122 204 810
Fragments arranged by size:
1122 810 204
SspI AAT'ATT
Cuts at: 0 1506 1710 1901 2136
Size: 1506 204 191 235
Fragments arranged by size:
1506 235 204 191
StyI C'CwwG_G
Cuts at: 0 335 1129 2136
Size: 335 794 1007
Fragments arranged by size:
1007 794 335
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 454 2136
Size: 454 1682
TatI w'GTAC_w
Cuts at: 0 1799 2136
Size: 1799 337
Tth111I GACn'n_nGTC
Cuts at: 0 174 2136
Size: 174 1962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 463 2136
Size: 463 1673
Enzymes that do cut:
AarI AcuI AgeI AleI AlfI AlwNI ApoI AseI
AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BpmI
Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI
BstAPI BstEII BstYI DraI DraIII DrdI EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRV FalI Hin4I HincII MmeI
MscI MslI MspA1I PflMI PpuMI PstI PvuII SacI
SapI SexAI SfcI SmlI SspI StyI TaqII TatI
Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AloI ApaI ApaLI AscI AsiSI BbeI BcgI BfrBI
BmrI BmtI BplI BsaBI BsaHI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BssHII BssSI BstBI BstXI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI EagI EciI
EcoNI EcoRI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TstI XbaI XhoI XmaI
XmnI ZraI