Restriction map for
pF1KE3190
(Linear) (Six-base) MAPSORT of: KIEE3190.seq Check: 4727 from: 1 to: 1326
KIEE3190.orf
With 182 enzymes: SgfI *
July 14, 2018 20:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 59 1326
Size: 59 1267
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 1307 1326
Size: 280 1027 19
Fragments arranged by size:
1027 280 19
AflIII A'CryG_T
Cuts at: 0 1223 1326
Size: 1223 103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 44 67 229 1326
Size: 44 23 162 1097
Fragments arranged by size:
1097 162 44 23
AlwNI CAG_nnn'CTG
Cuts at: 0 1285 1326
Size: 1285 41
ApoI r'AATT_y
Cuts at: 0 141 196 333 548 884 1237 1326
Size: 141 55 137 215 336 353 89
Fragments arranged by size:
353 336 215 141 137 89 55
AvrII C'CTAG_G
Cuts at: 0 177 1326
Size: 177 1149
BbsI GAAGACnn'nnnn_
Cuts at: 0 107 489 653 853 1326
Size: 107 382 164 200 473
Fragments arranged by size:
473 382 200 164 107
BglI GCCn_nnn'nGGC
Cuts at: 0 726 1326
Size: 726 600
BglII A'GATC_T
Cuts at: 0 1175 1326
Size: 1175 151
BmrI ACTGGGnnnn_n'
Cuts at: 0 1161 1326
Size: 1161 165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 523 624 1326
Size: 296 227 101 702
Fragments arranged by size:
702 296 227 101
BsaI GGTCTCn'nnnn_
Cuts at: 0 745 1326
Size: 745 581
BsaAI yAC'GTr
Cuts at: 0 784 1326
Size: 784 542
BsaBI GATnn'nnATC
Cuts at: 0 265 797 1326
Size: 265 532 529
Fragments arranged by size:
532 529 265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 59 1326
Size: 59 1267
BsrBI CCG'CTC
Cuts at: 0 57 1326
Size: 57 1269
BsrDI GCAATG_nn'
Cuts at: 0 384 1326
Size: 384 942
BsrFI r'CCGG_y
Cuts at: 0 4 1115 1326
Size: 4 1111 211
Fragments arranged by size:
1111 211 4
BsrGI T'GTAC_A
Cuts at: 0 381 1326
Size: 381 945
BstYI r'GATC_y
Cuts at: 0 1175 1326
Size: 1175 151
BtsI GCAGTG_nn'
Cuts at: 0 912 1326
Size: 912 414
EaeI y'GGCC_r
Cuts at: 0 2 1208 1326
Size: 2 1206 118
Fragments arranged by size:
1206 118 2
EarI CTCTTCn'nnn_
Cuts at: 0 299 679 821 1326
Size: 299 380 142 505
Fragments arranged by size:
505 380 299 142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 1307 1326
Size: 280 1027 19
Fragments arranged by size:
1027 280 19
EcoRI G'AATT_C
Cuts at: 0 884 1326
Size: 884 442
FseI GG_CCGG'CC
Cuts at: 0 1119 1326
Size: 1119 207
FspI TGC'GCA
Cuts at: 0 32 1326
Size: 32 1294
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 276 308 1326
Size: 276 32 1018
Fragments arranged by size:
1018 276 32
MfeI C'AATT_G
Cuts at: 0 476 1326
Size: 476 850
MslI CAynn'nnrTG
Cuts at: 0 663 1061 1326
Size: 663 398 265
Fragments arranged by size:
663 398 265
MspA1I CmG'CkG
Cuts at: 0 230 1136 1326
Size: 230 906 190
Fragments arranged by size:
906 230 190
NaeI GCC'GGC
Cuts at: 0 6 1117 1326
Size: 6 1111 209
Fragments arranged by size:
1111 209 6
NgoMIV G'CCGG_C
Cuts at: 0 4 1115 1326
Size: 4 1111 211
Fragments arranged by size:
1111 211 4
NspI r_CATG'y
Cuts at: 0 1227 1326
Size: 1227 99
PciI A'CATG_T
Cuts at: 0 1223 1326
Size: 1223 103
PfoI T'CCnGG_A
Cuts at: 0 276 1326
Size: 276 1050
PshAI GACnn'nnGTC
Cuts at: 0 152 230 1326
Size: 152 78 1096
Fragments arranged by size:
1096 152 78
PstI C_TGCA'G
Cuts at: 0 69 588 1326
Size: 69 519 738
Fragments arranged by size:
738 519 69
PvuII CAG'CTG
Cuts at: 0 230 1136 1326
Size: 230 906 190
Fragments arranged by size:
906 230 190
SfcI C'TryA_G
Cuts at: 0 65 584 1124 1326
Size: 65 519 540 202
Fragments arranged by size:
540 519 202 65
SmlI C'TyrA_G
Cuts at: 0 311 502 639 1326
Size: 311 191 137 687
Fragments arranged by size:
687 311 191 137
SspI AAT'ATT
Cuts at: 0 1010 1326
Size: 1010 316
StyI C'CwwG_G
Cuts at: 0 177 1326
Size: 177 1149
TatI w'GTAC_w
Cuts at: 0 381 952 1326
Size: 381 571 374
Fragments arranged by size:
571 381 374
XmnI GAAnn'nnTTC
Cuts at: 0 197 1326
Size: 197 1129
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApoI AvrII BbsI
BglI BglII BmrI BpuEI BsaI BsaAI BsaBI BspMI
BsrBI BsrDI BsrFI BsrGI BstYI BtsI EaeI EarI
Eco57MI EcoRI FseI FspI Hin4I MfeI MslI MspA1I
NaeI NgoMIV NspI PciI PfoI PshAI PstI PvuII
SfcI SmlI SspI StyI TatI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BlpI Bme1580I BmgBI BmtI BplI BpmI
Bpu10I BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoO109I EcoRV FalI FspAI HaeII HincII
HindIII HpaI KasI KpnI MluI MmeI MscI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI ZraI