(Linear) (Six-base) MAPSORT of: KIEE3148.seq  Check: 6677  from: 1  to: 1086



KIEE3148.orf

 With 182 enzymes: SgfI * 

                        November 18, 2016 17:23  ..

AccI GT'mk_AC

Cuts at:      0      29    1086 
   Size:         29    1057
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     967    1086 
   Size:        967     119
 
AflII C'TTAA_G

Cuts at:      0     432    1086 
   Size:        432     654
 
AgeI A'CCGG_T

Cuts at:      0     636    1086 
   Size:        636     450
 
AlwNI CAG_nnn'CTG

Cuts at:      0     205     588    1086 
   Size:        205     383     498

  Fragments arranged by size: 

                 498     383     205
 
ApoI r'AATT_y

Cuts at:      0     166     672     764    1023    1086 
   Size:        166     506      92     259      63

  Fragments arranged by size: 

                 506     259     166      92      63
 
BanI G'GyrC_C

Cuts at:      0     142     697    1086 
   Size:        142     555     389

  Fragments arranged by size: 

                 555     389     142
 
BanII G_rGCy'C

Cuts at:      0      39     359    1086 
   Size:         39     320     727

  Fragments arranged by size: 

                 727     320      39
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     436    1086 
   Size:        436     650
 
BlpI GC'TnA_GC

Cuts at:      0      38    1086 
   Size:         38    1048
 
BmgBI CAC'GTC

Cuts at:      0     794    1086 
   Size:        794     292
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      42     871    1086 
   Size:         42     829     215

  Fragments arranged by size: 

                 829     215      42
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196    1086 
   Size:        196     890
 
Bpu10I CC'TnA_GC

Cuts at:      0     365    1086 
   Size:        365     721
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     116    1086 
   Size:        116     970
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     910    1086 
   Size:        910     176
 
BsaHI Gr'CG_yC

Cuts at:      0      19    1086 
   Size:         19    1067
 
BsaWI w'CCGG_w

Cuts at:      0     636     919    1086 
   Size:        636     283     167

  Fragments arranged by size: 

                 636     283     167
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     143     173    1086 
   Size:        143      30     913

  Fragments arranged by size: 

                 913     143      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     359     821    1086 
   Size:        359     462     265

  Fragments arranged by size: 

                 462     359     265
 
Bsp1286I G_dGCh'C

Cuts at:      0      39     359     821    1086 
   Size:         39     320     462     265

  Fragments arranged by size: 

                 462     320     265      39
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     219    1086 
   Size:        219     867
 
BsrDI GCAATG_nn'

Cuts at:      0     937    1086 
   Size:        937     149
 
BsrFI r'CCGG_y

Cuts at:      0     636    1086 
   Size:        636     450
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     664    1086 
   Size:        664     422
 
BstYI r'GATC_y

Cuts at:      0     488    1079    1086 
   Size:        488     591       7

  Fragments arranged by size: 

                 591     488       7
 
BtgI C'CryG_G

Cuts at:      0     701    1086 
   Size:        701     385
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     287     322    1086 
   Size:        287      35     764

  Fragments arranged by size: 

                 764     287      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     842    1086 
   Size:        842     244
 
EarI CTCTTCn'nnn_

Cuts at:      0     368    1086 
   Size:        368     718
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     889    1086 
   Size:        889     197
 
EcoICRI GAG'CTC

Cuts at:      0     357    1086 
   Size:        357     729
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     967    1086 
   Size:        196     771     119

  Fragments arranged by size: 

                 771     196     119
 
HaeII r_GCGC'y

Cuts at:      0     381    1059    1086 
   Size:        381     678      27

  Fragments arranged by size: 

                 678     381      27
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     143     175     503     535    1086 
   Size:        143      32     328      32     551

  Fragments arranged by size: 

                 551     328     143      32      32
 
HincII GTy'rAC

Cuts at:      0      17     498    1086 
   Size:         17     481     588

  Fragments arranged by size: 

                 588     481      17
 
MfeI C'AATT_G

Cuts at:      0     617     758    1086 
   Size:        617     141     328

  Fragments arranged by size: 

                 617     328     141
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     417    1086 
   Size:        417     669
 
MslI CAynn'nnrTG

Cuts at:      0     802     864    1086 
   Size:        802      62     222

  Fragments arranged by size: 

                 802     222      62
 
NcoI C'CATG_G

Cuts at:      0     701    1086 
   Size:        701     385
 
NspI r_CATG'y

Cuts at:      0      28     789    1086 
   Size:         28     761     297

  Fragments arranged by size: 

                 761     297      28
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     558     588    1086 
   Size:        558      30     498

  Fragments arranged by size: 

                 558     498      30
 
PfoI T'CCnGG_A

Cuts at:      0     484    1086 
   Size:        484     602
 
PsiI TTA'TAA

Cuts at:      0     997    1040    1086 
   Size:        997      43      46

  Fragments arranged by size: 

                 997      46      43
 
SacI G_AGCT'C

Cuts at:      0     359    1086 
   Size:        359     727
 
SapI GCTCTTCn'nnn_

Cuts at:      0     368    1086 
   Size:        368     718
 
SgrAI Cr'CCGG_yG

Cuts at:      0     636    1086 
   Size:        636     450
 
SmlI C'TyrA_G

Cuts at:      0     131     432    1086 
   Size:        131     301     654

  Fragments arranged by size: 

                 654     301     131
 
SspI AAT'ATT

Cuts at:      0     908    1086 
   Size:        908     178
 
StyI C'CwwG_G

Cuts at:      0     701    1086 
   Size:        701     385
 
TatI w'GTAC_w

Cuts at:      0     243    1086 
   Size:        243     843
 
Tth111I GACn'n_nGTC

Cuts at:      0     157    1086 
   Size:        157     929
 

 Enzymes that do cut:

     AccI     AcuI    AflII     AgeI    AlwNI     ApoI     BanI    BanII
    BciVI     BlpI    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaHI    BsaWI    BsaXI  BsiHKAI Bsp1286I    BspMI    BsrDI    BsrFI
    BstXI    BstYI     BtgI    CspCI   DraIII     EarI     EciI  EcoICRI
  Eco57MI    HaeII    Hin4I   HincII     MfeI     MmeI     MslI     NcoI
     NspI    PflMI     PfoI     PsiI     SacI     SapI    SgrAI     SmlI
     SspI     StyI     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI     BclI
    BfrBI     BglI    BglII Bme1580I     BmtI     BplI    BsaAI    BsaBI
    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI
     EagI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     HpaI     KasI     KpnI     MluI     MscI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
    TaqII     TstI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI