Restriction map for
pF1KE3139
(Linear) (Six-base) MAPSORT of: KIEE3139.seq Check: 6899 from: 1 to: 1050
KIEE3139.orf
With 182 enzymes: SgfI *
March 1, 2013 10:20 ..
AatII G_ACGT'C
Cuts at: 0 775 806 1050
Size: 775 31 244
Fragments arranged by size:
775 244 31
AfeI AGC'GCT
Cuts at: 0 601 1050
Size: 601 449
AflIII A'CryG_T
Cuts at: 0 215 1050
Size: 215 835
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 235 1050
Size: 235 815
AlwNI CAG_nnn'CTG
Cuts at: 0 375 746 842 1050
Size: 375 371 96 208
Fragments arranged by size:
375 371 208 96
ApoI r'AATT_y
Cuts at: 0 985 1050
Size: 985 65
AvaI C'yCGr_G
Cuts at: 0 187 480 910 1050
Size: 187 293 430 140
Fragments arranged by size:
430 293 187 140
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 375 408 1050
Size: 375 33 642
Fragments arranged by size:
642 375 33
BamHI G'GATC_C
Cuts at: 0 620 1050
Size: 620 430
BanI G'GyrC_C
Cuts at: 0 106 1050
Size: 106 944
BanII G_rGCy'C
Cuts at: 0 917 1050
Size: 917 133
BbsI GAAGACnn'nnnn_
Cuts at: 0 798 984 1050
Size: 798 186 66
Fragments arranged by size:
798 186 66
BbvCI CC'TCA_GC
Cuts at: 0 20 835 1050
Size: 20 815 215
Fragments arranged by size:
815 215 20
BciVI GTATCCnnnnn_n'
Cuts at: 0 403 1050
Size: 403 647
BglII A'GATC_T
Cuts at: 0 941 1050
Size: 941 109
BlpI GC'TnA_GC
Cuts at: 0 667 1050
Size: 667 383
Bme1580I G_kGCm'C
Cuts at: 0 340 494 1050
Size: 340 154 556
Fragments arranged by size:
556 340 154
BmgBI CAC'GTC
Cuts at: 0 507 933 959 1050
Size: 507 426 26 91
Fragments arranged by size:
507 426 91 26
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 48 432 1050
Size: 48 384 618
Fragments arranged by size:
618 384 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 484 1050
Size: 18 466 566
Fragments arranged by size:
566 466 18
Bpu10I CC'TnA_GC
Cuts at: 0 20 835 1050
Size: 20 815 215
Fragments arranged by size:
815 215 20
BsaAI yAC'GTr
Cuts at: 0 489 1050
Size: 489 561
BsaHI Gr'CG_yC
Cuts at: 0 296 772 803 881 1050
Size: 296 476 31 78 169
Fragments arranged by size:
476 296 169 78 31
BsaWI w'CCGG_w
Cuts at: 0 388 575 1050
Size: 388 187 475
Fragments arranged by size:
475 388 187
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 439 469 1050
Size: 439 30 581
Fragments arranged by size:
581 439 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 346 1050
Size: 37 309 704
Fragments arranged by size:
704 309 37
BseYI C'CCAG_C
Cuts at: 0 7 1050
Size: 7 1043
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 712 922 1050
Size: 266 446 210 128
Fragments arranged by size:
446 266 210 128
BsiHKAI G_wGCw'C
Cuts at: 0 130 785 917 1050
Size: 130 655 132 133
Fragments arranged by size:
655 133 132 130
BsiWI C'GTAC_G
Cuts at: 0 240 1050
Size: 240 810
BsmBI CGTCTCn'nnnn_
Cuts at: 0 538 1050
Size: 538 512
Bsp1286I G_dGCh'C
Cuts at: 0 130 340 494 785 917 1050
Size: 130 210 154 291 132 133
Fragments arranged by size:
291 210 154 133 132 130
BspEI T'CCGG_A
Cuts at: 0 575 1050
Size: 575 475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 652 1050
Size: 652 398
BsrFI r'CCGG_y
Cuts at: 0 109 883 1050
Size: 109 774 167
Fragments arranged by size:
774 167 109
BssHII G'CGCG_C
Cuts at: 0 411 1050
Size: 411 639
BssSI C'ACGA_G
Cuts at: 0 718 1050
Size: 718 332
BstAPI GCAn_nnn'nTGC
Cuts at: 0 507 1050
Size: 507 543
BstEII G'GTnAC_C
Cuts at: 0 474 1050
Size: 474 576
BstXI CCAn_nnnn'nTGG
Cuts at: 0 42 1050
Size: 42 1008
BstYI r'GATC_y
Cuts at: 0 564 620 941 1050
Size: 564 56 321 109
Fragments arranged by size:
564 321 109 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 642 1050
Size: 642 408
BtsI GCAGTG_nn'
Cuts at: 0 624 1050
Size: 624 426
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1020 1050
Size: 1020 30
EaeI y'GGCC_r
Cuts at: 0 233 1050
Size: 233 817
EarI CTCTTCn'nnn_
Cuts at: 0 118 173 790 1050
Size: 118 55 617 260
Fragments arranged by size:
617 260 118 55
EcoICRI GAG'CTC
Cuts at: 0 915 1050
Size: 915 135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 484 1050
Size: 18 466 566
Fragments arranged by size:
566 466 18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 822 1050
Size: 822 228
HaeII r_GCGC'y
Cuts at: 0 471 603 667 674 1050
Size: 471 132 64 7 376
Fragments arranged by size:
471 376 132 64 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 154 186 1050
Size: 154 32 864
Fragments arranged by size:
864 154 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 482 1050
Size: 338 144 568
Fragments arranged by size:
568 338 144
MscI TGG'CCA
Cuts at: 0 235 1050
Size: 235 815
MslI CAynn'nnrTG
Cuts at: 0 898 1050
Size: 898 152
MspA1I CmG'CkG
Cuts at: 0 372 1050
Size: 372 678
NaeI GCC'GGC
Cuts at: 0 885 1050
Size: 885 165
NgoMIV G'CCGG_C
Cuts at: 0 883 1050
Size: 883 167
NruI TCG'CGA
Cuts at: 0 519 1050
Size: 519 531
NspI r_CATG'y
Cuts at: 0 717 924 1050
Size: 717 207 126
Fragments arranged by size:
717 207 126
PfoI T'CCnGG_A
Cuts at: 0 118 848 1050
Size: 118 730 202
Fragments arranged by size:
730 202 118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 535 567 1050
Size: 535 32 483
Fragments arranged by size:
535 483 32
PspXI vC'TCGA_Gb
Cuts at: 0 187 910 1050
Size: 187 723 140
Fragments arranged by size:
723 187 140
SacI G_AGCT'C
Cuts at: 0 917 1050
Size: 917 133
SapI GCTCTTCn'nnn_
Cuts at: 0 118 790 1050
Size: 118 672 260
Fragments arranged by size:
672 260 118
SmaI CCC'GGG
Cuts at: 0 482 1050
Size: 482 568
SmlI C'TyrA_G
Cuts at: 0 187 910 1050
Size: 187 723 140
Fragments arranged by size:
723 187 140
SphI G_CATG'C
Cuts at: 0 717 1050
Size: 717 333
StuI AGG'CCT
Cuts at: 0 400 1050
Size: 400 650
StyI C'CwwG_G
Cuts at: 0 347 1050
Size: 347 703
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 94 1050
Size: 94 956
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 248 280 1050
Size: 248 32 770
Fragments arranged by size:
770 248 32
Tth111I GACn'n_nGTC
Cuts at: 0 1000 1050
Size: 1000 50
XhoI C'TCGA_G
Cuts at: 0 187 910 1050
Size: 187 723 140
Fragments arranged by size:
723 187 140
XmaI C'CCGG_G
Cuts at: 0 480 1050
Size: 480 570
ZraI GAC'GTC
Cuts at: 0 773 804 1050
Size: 773 31 246
Fragments arranged by size:
773 246 31
Enzymes that do cut:
AatII AfeI AflIII AlfI AlwNI ApoI AvaI BaeI
BamHI BanI BanII BbsI BbvCI BciVI BglII BlpI
Bme1580I BmgBI BplI BpmI Bpu10I BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmBI Bsp1286I
BspEI BspMI BsrFI BssHII BssSI BstAPI BstEII BstXI
BstYI BtgZI BtsI CspCI EaeI EarI EcoICRI Eco57MI
FalI HaeII Hin4I MmeI MscI MslI MspA1I NaeI
NgoMIV NruI NspI PfoI PpiI PspXI SacI SapI
SmaI SmlI SphI StuI StyI TaqII TstI Tth111I
XhoI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BbeI BcgI BclI BfrBI BglI BmrI BmtI BpuEI
BsaI BsaBI BsiEI BsmI BspHI BsrBI BsrDI BsrGI
BstBI BstZ17I Bsu36I BtgI ClaI DraI DraIII DrdI
EagI EciI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
NarI NcoI NdeI NheI NotI NsiI PacI PasI
PciI PflMI PmeI PmlI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI TatI XbaI XcmI
XmnI