Restriction map for
pF1KE3103
(Linear) (Six-base) MAPSORT of: KIEE3103.seq Check: 6861 from: 1 to: 807
KIEE3103.orf
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 402 807
Size: 402 405
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 807
Size: 165 642
ApaI G_GGCC'C
Cuts at: 0 123 290 331 337 559 807
Size: 123 167 41 6 222 248
Fragments arranged by size:
248 222 167 123 41 6
AscI GG'CGCG_CC
Cuts at: 0 41 807
Size: 41 766
AvaI C'yCGr_G
Cuts at: 0 330 530 574 807
Size: 330 200 44 233
Fragments arranged by size:
330 233 200 44
AvrII C'CTAG_G
Cuts at: 0 123 807
Size: 123 684
BanI G'GyrC_C
Cuts at: 0 136 807
Size: 136 671
BanII G_rGCy'C
Cuts at: 0 123 242 290 331 337 368 559 807
Size: 123 119 48 41 6 31 191 248
Fragments arranged by size:
248 191 123 119 48 41 31 6
BbeI G_GCGC'C
Cuts at: 0 140 807
Size: 140 667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 588 622 807
Size: 588 34 185
Fragments arranged by size:
588 185 34
BglI GCCn_nnn'nGGC
Cuts at: 0 26 89 326 807
Size: 26 63 237 481
Fragments arranged by size:
481 237 63 26
Bme1580I G_kGCm'C
Cuts at: 0 123 215 290 331 337 559 807
Size: 123 92 75 41 6 222 248
Fragments arranged by size:
248 222 123 92 75 41 6
BmgBI CAC'GTC
Cuts at: 0 476 807
Size: 476 331
BmrI ACTGGGnnnn_n'
Cuts at: 0 381 807
Size: 381 426
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 313 807
Size: 241 72 494
Fragments arranged by size:
494 241 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 807
Size: 340 467
BsaHI Gr'CG_yC
Cuts at: 0 137 807
Size: 137 670
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 181 211 807
Size: 181 30 596
Fragments arranged by size:
596 181 30
BseYI C'CCAG_C
Cuts at: 0 763 807
Size: 763 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 682 807
Size: 682 125
BsiEI CG_ry'CG
Cuts at: 0 452 785 807
Size: 452 333 22
Fragments arranged by size:
452 333 22
BsiHKAI G_wGCw'C
Cuts at: 0 242 807
Size: 242 565
Bsp1286I G_dGCh'C
Cuts at: 0 123 215 242 290 331 337 368 559
Size: 123 92 27 48 41 6 31 191
Cuts at: 559 807
Size: 248
Fragments arranged by size:
248 191 123 92 48 41 31 27
6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 402 807
Size: 402 405
BsrBI CCG'CTC
Cuts at: 0 343 807
Size: 343 464
BsrFI r'CCGG_y
Cuts at: 0 544 581 733 780 807
Size: 544 37 152 47 27
Fragments arranged by size:
544 152 47 37 27
BssHII G'CGCG_C
Cuts at: 0 34 41 316 807
Size: 34 7 275 491
Fragments arranged by size:
491 275 34 7
BtgI C'CryG_G
Cuts at: 0 102 200 434 564 807
Size: 102 98 234 130 243
Fragments arranged by size:
243 234 130 102 98
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 591 807
Size: 591 216
EaeI y'GGCC_r
Cuts at: 0 203 416 449 731 782 807
Size: 203 213 33 282 51 25
Fragments arranged by size:
282 213 203 51 33 25
EagI C'GGCC_G
Cuts at: 0 449 782 807
Size: 449 333 25
Fragments arranged by size:
449 333 25
EarI CTCTTCn'nnn_
Cuts at: 0 19 247 259 532 807
Size: 19 228 12 273 275
Fragments arranged by size:
275 273 228 19 12
EcoICRI GAG'CTC
Cuts at: 0 240 807
Size: 240 567
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 241 313 807
Size: 165 76 72 494
Fragments arranged by size:
494 165 76 72
EcoO109I rG'GnC_Cy
Cuts at: 0 119 120 286 287 334 555 726 807
Size: 119 1 166 1 47 221 171 81
Fragments arranged by size:
221 171 166 119 81 47 1 1
FseI GG_CCGG'CC
Cuts at: 0 737 807
Size: 737 70
FspI TGC'GCA
Cuts at: 0 426 616 807
Size: 426 190 191
Fragments arranged by size:
426 191 190
FspAI rTGC'GCAy
Cuts at: 0 426 807
Size: 426 381
HaeII r_GCGC'y
Cuts at: 0 8 140 173 188 545 807
Size: 8 132 33 15 357 262
Fragments arranged by size:
357 262 132 33 15 8
KasI G'GCGC_C
Cuts at: 0 136 807
Size: 136 671
MslI CAynn'nnrTG
Cuts at: 0 610 807
Size: 610 197
MspA1I CmG'CkG
Cuts at: 0 104 566 588 759 807
Size: 104 462 22 171 48
Fragments arranged by size:
462 171 104 48 22
NaeI GCC'GGC
Cuts at: 0 546 735 782 807
Size: 546 189 47 25
Fragments arranged by size:
546 189 47 25
NarI GG'CG_CC
Cuts at: 0 137 807
Size: 137 670
NcoI C'CATG_G
Cuts at: 0 200 807
Size: 200 607
NgoMIV G'CCGG_C
Cuts at: 0 544 733 780 807
Size: 544 189 47 27
Fragments arranged by size:
544 189 47 27
NotI GC'GGCC_GC
Cuts at: 0 449 807
Size: 449 358
PflMI CCAn_nnn'nTGG
Cuts at: 0 708 807
Size: 708 99
PpuMI rG'GwC_Cy
Cuts at: 0 726 807
Size: 726 81
PspOMI G'GGCC_C
Cuts at: 0 119 286 327 333 555 807
Size: 119 167 41 6 222 252
Fragments arranged by size:
252 222 167 119 41 6
PvuII CAG'CTG
Cuts at: 0 759 807
Size: 759 48
RsrII CG'GwC_CG
Cuts at: 0 561 807
Size: 561 246
SacI G_AGCT'C
Cuts at: 0 242 807
Size: 242 565
SacII CC_GC'GG
Cuts at: 0 105 567 807
Size: 105 462 240
Fragments arranged by size:
462 240 105
SapI GCTCTTCn'nnn_
Cuts at: 0 19 247 532 807
Size: 19 228 285 275
Fragments arranged by size:
285 275 228 19
SfoI GGC'GCC
Cuts at: 0 138 807
Size: 138 669
SmaI CCC'GGG
Cuts at: 0 332 532 807
Size: 332 200 275
Fragments arranged by size:
332 275 200
SmlI C'TyrA_G
Cuts at: 0 355 807
Size: 355 452
SrfI GCCC'GGGC
Cuts at: 0 332 807
Size: 332 475
StyI C'CwwG_G
Cuts at: 0 123 200 323 807
Size: 123 77 123 484
Fragments arranged by size:
484 123 123 77
XmaI C'CCGG_G
Cuts at: 0 330 530 807
Size: 330 200 277
Fragments arranged by size:
330 277 200
Enzymes that do cut:
AarI AcuI ApaI AscI AvaI AvrII BanI BanII
BbeI BcgI BglI Bme1580I BmgBI BmrI BpmI BpuEI
BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrFI BssHII BtgI BtgZI EaeI EagI EarI
EcoICRI Eco57MI EcoO109I FseI FspI FspAI HaeII KasI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI PflMI
PpuMI PspOMI PvuII RsrII SacI SacII SapI SfoI
SmaI SmlI SrfI StyI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI BaeI BamHI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI BmtI BplI Bpu10I BsaI BsaAI BsaBI
BsaWI BseRI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EciI
EcoNI EcoRI EcoRV FalI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI NdeI NheI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PstI PvuI SalI
SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SnaBI SpeI SphI SspI StuI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmnI ZraI