(Linear) (Six-base) MAPSORT of: KIEE3023.seq  Check: 6243  from: 1  to: 414



KIEE3023.orf

 With 182 enzymes: SgfI * 

                        June 20, 2019 10:05  ..

AccI GT'mk_AC

Cuts at:      0      79     414 
   Size:         79     335
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     357     390     414 
   Size:        357      33      24

  Fragments arranged by size: 

                 357      33      24
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      90     120     414 
   Size:         90      30     294

  Fragments arranged by size: 

                 294      90      30
 
BstZ17I GTA'TAC

Cuts at:      0      80     414 
   Size:         80     334
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      67     414 
   Size:         67     347
 
EcoRV GAT'ATC

Cuts at:      0     165     414 
   Size:        165     249
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     215     247     248     280     395     414 
   Size:        215      32       1      32     115      19

  Fragments arranged by size: 

                 215     115      32      32      19       1
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     414 
   Size:         94     320
 
NspI r_CATG'y

Cuts at:      0      44     414 
   Size:         44     370
 
SfcI C'TryA_G

Cuts at:      0     234     242     333     414 
   Size:        234       8      91      81

  Fragments arranged by size: 

                 234      91      81       8
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      90     122     414 
   Size:         90      32     292

  Fragments arranged by size: 

                 292      90      32
 

 Enzymes that do cut:

     AccI     BaeI    BsaXI  BstZ17I    BtgZI    EcoRV    Hin4I     MmeI
     NspI     SfcI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I
     BtgI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI