Restriction map for
pF1KE2788
(Linear) (Six-base) MAPSORT of: KIEE2788.seq Check: 2693 from: 1 to: 903
KIEE2788.orf
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 66 903
Size: 66 837
AccI GT'mk_AC
Cuts at: 0 746 903
Size: 746 157
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 487 903
Size: 182 305 416
Fragments arranged by size:
416 305 182
AlwNI CAG_nnn'CTG
Cuts at: 0 717 903
Size: 717 186
ApoI r'AATT_y
Cuts at: 0 105 903
Size: 105 798
AseI AT'TA_AT
Cuts at: 0 410 903
Size: 410 493
BbsI GAAGACnn'nnnn_
Cuts at: 0 751 903
Size: 751 152
BmrI ACTGGGnnnn_n'
Cuts at: 0 512 903
Size: 512 391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 661 805 903
Size: 72 589 144 98
Fragments arranged by size:
589 144 98 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 903
Size: 49 854
BsaI GGTCTCn'nnnn_
Cuts at: 0 319 781 903
Size: 319 462 122
Fragments arranged by size:
462 319 122
BsaAI yAC'GTr
Cuts at: 0 165 903
Size: 165 738
BsaHI Gr'CG_yC
Cuts at: 0 721 903
Size: 721 182
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 903
Size: 112 791
BseYI C'CCAG_C
Cuts at: 0 82 903
Size: 82 821
BsmI GAATG_Cn'
Cuts at: 0 204 903
Size: 204 699
BspHI T'CATG_A
Cuts at: 0 693 734 903
Size: 693 41 169
Fragments arranged by size:
693 169 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 66 903
Size: 66 837
BsrDI GCAATG_nn'
Cuts at: 0 897 903
Size: 897 6
BtgI C'CryG_G
Cuts at: 0 491 903
Size: 491 412
BtsI GCAGTG_nn'
Cuts at: 0 629 903
Size: 629 274
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 37 72 903
Size: 37 35 831
Fragments arranged by size:
831 37 35
EaeI y'GGCC_r
Cuts at: 0 404 903
Size: 404 499
EarI CTCTTCn'nnn_
Cuts at: 0 89 201 903
Size: 89 112 702
Fragments arranged by size:
702 112 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 182 487 661 805 903
Size: 72 110 305 174 144 98
Fragments arranged by size:
305 174 144 110 98 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 418 450 794 826 903
Size: 418 32 344 32 77
Fragments arranged by size:
418 344 77 32 32
HincII GTy'rAC
Cuts at: 0 839 903
Size: 839 64
MfeI C'AATT_G
Cuts at: 0 829 903
Size: 829 74
MscI TGG'CCA
Cuts at: 0 406 903
Size: 406 497
MspA1I CmG'CkG
Cuts at: 0 546 903
Size: 546 357
NcoI C'CATG_G
Cuts at: 0 491 903
Size: 491 412
PflMI CCAn_nnn'nTGG
Cuts at: 0 402 717 903
Size: 402 315 186
Fragments arranged by size:
402 315 186
PsiI TTA'TAA
Cuts at: 0 68 371 903
Size: 68 303 532
Fragments arranged by size:
532 303 68
PstI C_TGCA'G
Cuts at: 0 254 297 903
Size: 254 43 606
Fragments arranged by size:
606 254 43
ScaI AGT'ACT
Cuts at: 0 34 903
Size: 34 869
SfcI C'TryA_G
Cuts at: 0 250 293 903
Size: 250 43 610
Fragments arranged by size:
610 250 43
SmlI C'TyrA_G
Cuts at: 0 28 903
Size: 28 875
StyI C'CwwG_G
Cuts at: 0 491 903
Size: 491 412
TatI w'GTAC_w
Cuts at: 0 32 671 903
Size: 32 639 232
Fragments arranged by size:
639 232 32
XbaI T'CTAG_A
Cuts at: 0 769 903
Size: 769 134
Enzymes that do cut:
AarI AccI AcuI AlwNI ApoI AseI BbsI BmrI
BpmI BpuEI BsaI BsaAI BsaHI BseRI BseYI BsmI
BspHI BspMI BsrDI BtgI BtsI CspCI EaeI EarI
Eco57MI Hin4I HincII MfeI MscI MspA1I NcoI PflMI
PsiI PstI ScaI SfcI SmlI StyI TatI XbaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmtI BplI Bpu10I BsaBI BsaWI BsaXI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BspEI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MluI MmeI
MslI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI Tth111I XcmI
XhoI XmaI XmnI ZraI