Restriction map for
pF1KE2761
(Linear) (Six-base) MAPSORT of: KIEE2761.seq Check: 1240 from: 1 to: 2505
KIEE2761.orf
With 182 enzymes: SgfI *
October 3, 2019 17:18 ..
AccI GT'mk_AC
Cuts at: 0 1107 1344 1731 2505
Size: 1107 237 387 774
Fragments arranged by size:
1107 774 387 237
Acc65I G'GTAC_C
Cuts at: 0 544 2505
Size: 544 1961
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1697 2505
Size: 1697 808
AflIII A'CryG_T
Cuts at: 0 104 2505
Size: 104 2401
AhdI GACnn_n'nnGTC
Cuts at: 0 2425 2505
Size: 2425 80
AlwNI CAG_nnn'CTG
Cuts at: 0 815 2505
Size: 815 1690
ApoI r'AATT_y
Cuts at: 0 243 1378 2046 2505
Size: 243 1135 668 459
Fragments arranged by size:
1135 668 459 243
AvaI C'yCGr_G
Cuts at: 0 668 716 2505
Size: 668 48 1789
Fragments arranged by size:
1789 668 48
BanI G'GyrC_C
Cuts at: 0 544 1573 2505
Size: 544 1029 932
Fragments arranged by size:
1029 932 544
BanII G_rGCy'C
Cuts at: 0 2059 2505
Size: 2059 446
BbsI GAAGACnn'nnnn_
Cuts at: 0 1618 2503 2505
Size: 1618 885 2
Fragments arranged by size:
1618 885 2
BbvCI CC'TCA_GC
Cuts at: 0 2279 2505
Size: 2279 226
BclI T'GATC_A
Cuts at: 0 944 2505
Size: 944 1561
BfrBI ATG'CAT
Cuts at: 0 24 209 2505
Size: 24 185 2296
Fragments arranged by size:
2296 185 24
BglII A'GATC_T
Cuts at: 0 125 2505
Size: 125 2380
BlpI GC'TnA_GC
Cuts at: 0 703 1418 2505
Size: 703 715 1087
Fragments arranged by size:
1087 715 703
BmgBI CAC'GTC
Cuts at: 0 1111 1729 2505
Size: 1111 618 776
Fragments arranged by size:
1111 776 618
BmrI ACTGGGnnnn_n'
Cuts at: 0 1609 2505
Size: 1609 896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1510 2505
Size: 1510 995
Bpu10I CC'TnA_GC
Cuts at: 0 2279 2505
Size: 2279 226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 2505
Size: 288 2217
BsaAI yAC'GTr
Cuts at: 0 1633 2505
Size: 1633 872
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2459 2489 2505
Size: 2459 30 16
Fragments arranged by size:
2459 30 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 824 1248 2505
Size: 824 424 1257
Fragments arranged by size:
1257 824 424
BseYI C'CCAG_C
Cuts at: 0 636 1428 2068 2383 2505
Size: 636 792 640 315 122
Fragments arranged by size:
792 640 636 315 122
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2208 2505
Size: 2208 297
BsmI GAATG_Cn'
Cuts at: 0 26 1365 2505
Size: 26 1339 1140
Fragments arranged by size:
1339 1140 26
Bsp1286I G_dGCh'C
Cuts at: 0 2059 2505
Size: 2059 446
BspHI T'CATG_A
Cuts at: 0 623 1461 2505
Size: 623 838 1044
Fragments arranged by size:
1044 838 623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1926 2505
Size: 1926 579
BsrDI GCAATG_nn'
Cuts at: 0 2123 2505
Size: 2123 382
BssSI C'ACGA_G
Cuts at: 0 473 1607 1855 2505
Size: 473 1134 248 650
Fragments arranged by size:
1134 650 473 248
BstAPI GCAn_nnn'nTGC
Cuts at: 0 427 2505
Size: 427 2078
BstBI TT'CG_AA
Cuts at: 0 18 2505
Size: 18 2487
BstYI r'GATC_y
Cuts at: 0 125 2505
Size: 125 2380
BtgI C'CryG_G
Cuts at: 0 1802 2063 2505
Size: 1802 261 442
Fragments arranged by size:
1802 442 261
BtsI GCAGTG_nn'
Cuts at: 0 183 1694 2505
Size: 183 1511 811
Fragments arranged by size:
1511 811 183
EarI CTCTTCn'nnn_
Cuts at: 0 155 1154 1938 2505
Size: 155 999 784 567
Fragments arranged by size:
999 784 567 155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1510 1697 2505
Size: 1510 187 808
Fragments arranged by size:
1510 808 187
EcoO109I rG'GnC_Cy
Cuts at: 0 493 2505
Size: 493 2012
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1026 2505
Size: 1026 1479
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 765 797 810 842 1018 1050 1261 1293
Size: 765 32 13 32 176 32 211 32
Cuts at: 1293 1443 1475 2411 2443 2505
Size: 150 32 936 32 62
Fragments arranged by size:
936 765 211 176 150 62 32 32
32 32 32 32 13
HincII GTy'rAC
Cuts at: 0 253 2034 2505
Size: 253 1781 471
Fragments arranged by size:
1781 471 253
HindIII A'AGCT_T
Cuts at: 0 368 1504 2505
Size: 368 1136 1001
Fragments arranged by size:
1136 1001 368
KpnI G_GTAC'C
Cuts at: 0 548 2505
Size: 548 1957
MfeI C'AATT_G
Cuts at: 0 1098 2505
Size: 1098 1407
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 464 1421 2505
Size: 115 349 957 1084
Fragments arranged by size:
1084 957 349 115
MslI CAynn'nnrTG
Cuts at: 0 88 103 1466 1807 2505
Size: 88 15 1363 341 698
Fragments arranged by size:
1363 698 341 88 15
MspA1I CmG'CkG
Cuts at: 0 2222 2505
Size: 2222 283
NcoI C'CATG_G
Cuts at: 0 1802 2063 2505
Size: 1802 261 442
Fragments arranged by size:
1802 442 261
NsiI A_TGCA'T
Cuts at: 0 26 211 2505
Size: 26 185 2294
Fragments arranged by size:
2294 185 26
NspI r_CATG'y
Cuts at: 0 108 597 2321 2505
Size: 108 489 1724 184
Fragments arranged by size:
1724 489 184 108
PasI CC'CwG_GG
Cuts at: 0 50 1316 2505
Size: 50 1266 1189
Fragments arranged by size:
1266 1189 50
PciI A'CATG_T
Cuts at: 0 104 2505
Size: 104 2401
PmlI CAC'GTG
Cuts at: 0 1633 2505
Size: 1633 872
PpuMI rG'GwC_Cy
Cuts at: 0 493 2505
Size: 493 2012
PsiI TTA'TAA
Cuts at: 0 187 1198 1550 2505
Size: 187 1011 352 955
Fragments arranged by size:
1011 955 352 187
PstI C_TGCA'G
Cuts at: 0 1650 2505
Size: 1650 855
PvuII CAG'CTG
Cuts at: 0 2222 2505
Size: 2222 283
ScaI AGT'ACT
Cuts at: 0 1283 2505
Size: 1283 1222
SfcI C'TryA_G
Cuts at: 0 1070 1345 1646 1732 2237 2505
Size: 1070 275 301 86 505 268
Fragments arranged by size:
1070 505 301 275 268 86
SmlI C'TyrA_G
Cuts at: 0 303 2505
Size: 303 2202
SphI G_CATG'C
Cuts at: 0 2321 2505
Size: 2321 184
SspI AAT'ATT
Cuts at: 0 468 2505
Size: 468 2037
StyI C'CwwG_G
Cuts at: 0 650 1802 1982 2063 2478 2505
Size: 650 1152 180 81 415 27
Fragments arranged by size:
1152 650 415 180 81 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2245 2505
Size: 2245 260
TatI w'GTAC_w
Cuts at: 0 319 844 1004 1281 1903 2505
Size: 319 525 160 277 622 602
Fragments arranged by size:
622 602 525 319 277 160
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1593 1625 2505
Size: 1593 32 880
Fragments arranged by size:
1593 880 32
Enzymes that do cut:
AccI Acc65I AcuI AflIII AhdI AlwNI ApoI AvaI
BanI BanII BbsI BbvCI BclI BfrBI BglII BlpI
BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaXI BseRI
BseYI BsgI BsmI Bsp1286I BspHI BspMI BsrDI BssSI
BstAPI BstBI BstYI BtgI BtsI EarI Eco57MI EcoO109I
FalI Hin4I HincII HindIII KpnI MfeI MmeI MslI
MspA1I NcoI NsiI NspI PasI PciI PmlI PpuMI
PsiI PstI PvuII ScaI SfcI SmlI SphI SspI
StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BcgI BciVI BglI Bme1580I BmtI BplI
BsaI BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmBI
BspEI BsrBI BsrFI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HpaI KasI MluI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PflMI PfoI
PmeI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI
SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI