(Linear) (Six-base) MAPSORT of: KIEE2755.seq  Check: 7120  from: 1  to: 399



KIEE2755.orf

 With 182 enzymes: SgfI * 

                        June 30, 2019 14:46  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     166     399 
   Size:        166     233
 
ApaLI G'TGCA_C

Cuts at:      0      23     399 
   Size:         23     376
 
ApoI r'AATT_y

Cuts at:      0     387     399 
   Size:        387      12
 
BanII G_rGCy'C

Cuts at:      0      64     399 
   Size:         64     335
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     361     399 
   Size:        361      38
 
Bme1580I G_kGCm'C

Cuts at:      0      27     399 
   Size:         27     372
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      30     134     399 
   Size:         30     104     265

  Fragments arranged by size: 

                 265     104      30
 
Bpu10I CC'TnA_GC

Cuts at:      0      63     399 
   Size:         63     336
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     269     399 
   Size:        269     130
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     399 
   Size:        148     251
 
BsiHKAI G_wGCw'C

Cuts at:      0      27      64     399 
   Size:         27      37     335

  Fragments arranged by size: 

                 335      37      27
 
Bsp1286I G_dGCh'C

Cuts at:      0      27      64     399 
   Size:         27      37     335

  Fragments arranged by size: 

                 335      37      27
 
BsrFI r'CCGG_y

Cuts at:      0     377     399 
   Size:        377      22
 
BtgI C'CryG_G

Cuts at:      0     356     399 
   Size:        356      43
 
EarI CTCTTCn'nnn_

Cuts at:      0     146     399 
   Size:        146     253
 
EcoICRI GAG'CTC

Cuts at:      0      62     399 
   Size:         62     337
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      30     134     399 
   Size:         30     104     265

  Fragments arranged by size: 

                 265     104      30
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     115     147     265     297     335     367     399 
   Size:        115      32     118      32      38      32      32

  Fragments arranged by size: 

                 118     115      38      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     134     399 
   Size:        134     265
 
MslI CAynn'nnrTG

Cuts at:      0     124     399 
   Size:        124     275
 
MspA1I CmG'CkG

Cuts at:      0     286     399 
   Size:        286     113
 
NcoI C'CATG_G

Cuts at:      0     356     399 
   Size:        356      43
 
PvuII CAG'CTG

Cuts at:      0     286     399 
   Size:        286     113
 
SacI G_AGCT'C

Cuts at:      0      64     399 
   Size:         64     335
 
SfcI C'TryA_G

Cuts at:      0     293     399 
   Size:        293     106
 
StuI AGG'CCT

Cuts at:      0     103     399 
   Size:        103     296
 
StyI C'CwwG_G

Cuts at:      0     356     399 
   Size:        356      43
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     153     399 
   Size:        153     246
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      17      49     399 
   Size:         17      32     350

  Fragments arranged by size: 

                 350      32      17
 

 Enzymes that do cut:

    AlwNI    ApaLI     ApoI    BanII    BciVI Bme1580I     BpmI   Bpu10I
     BsaI     BsgI  BsiHKAI Bsp1286I    BsrFI     BtgI     EarI  EcoICRI
  Eco57MI    Hin4I   HincII     MslI   MspA1I     NcoI    PvuII     SacI
     SfcI     StuI     StyI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI