Restriction map for
pF1KE2721
(Linear) (Six-base) MAPSORT of: KIEE2721.seq Check: 8809 from: 1 to: 591
KIEE2721.orf
With 182 enzymes: SgfI *
June 6, 2018 17:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 84 562 591
Size: 82 2 478 29
Fragments arranged by size:
478 82 29 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 310 591
Size: 310 281
AlwNI CAG_nnn'CTG
Cuts at: 0 399 591
Size: 399 192
ApaI G_GGCC'C
Cuts at: 0 10 591
Size: 10 581
AvaI C'yCGr_G
Cuts at: 0 126 228 591
Size: 126 102 363
Fragments arranged by size:
363 126 102
BanI G'GyrC_C
Cuts at: 0 586 591
Size: 586 5
BanII G_rGCy'C
Cuts at: 0 10 485 591
Size: 10 475 106
Fragments arranged by size:
475 106 10
BbeI G_GCGC'C
Cuts at: 0 590 591
Size: 590 1
BglI GCCn_nnn'nGGC
Cuts at: 0 274 591
Size: 274 317
BlpI GC'TnA_GC
Cuts at: 0 25 591
Size: 25 566
Bme1580I G_kGCm'C
Cuts at: 0 10 591
Size: 10 581
BmgBI CAC'GTC
Cuts at: 0 303 591
Size: 303 288
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 570 591
Size: 570 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 409 591
Size: 58 351 182
Fragments arranged by size:
351 182 58
BsaAI yAC'GTr
Cuts at: 0 384 591
Size: 384 207
BsaHI Gr'CG_yC
Cuts at: 0 587 591
Size: 587 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 154 439 591
Size: 154 285 152
Fragments arranged by size:
285 154 152
BseYI C'CCAG_C
Cuts at: 0 43 591
Size: 43 548
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 292 364 385 591
Size: 283 9 72 21 206
Fragments arranged by size:
283 206 72 21 9
BsiHKAI G_wGCw'C
Cuts at: 0 55 83 591
Size: 55 28 508
Fragments arranged by size:
508 55 28
Bsp1286I G_dGCh'C
Cuts at: 0 10 55 83 485 591
Size: 10 45 28 402 106
Fragments arranged by size:
402 106 45 28 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 317 591
Size: 317 274
BsrBI CCG'CTC
Cuts at: 0 29 591
Size: 29 562
BsrFI r'CCGG_y
Cuts at: 0 199 583 591
Size: 199 384 8
Fragments arranged by size:
384 199 8
BssSI C'ACGA_G
Cuts at: 0 117 246 591
Size: 117 129 345
Fragments arranged by size:
345 129 117
BstAPI GCAn_nnn'nTGC
Cuts at: 0 270 318 399 591
Size: 270 48 81 192
Fragments arranged by size:
270 192 81 48
BstXI CCAn_nnnn'nTGG
Cuts at: 0 18 591
Size: 18 573
BtsI GCAGTG_nn'
Cuts at: 0 376 591
Size: 376 215
EarI CTCTTCn'nnn_
Cuts at: 0 43 58 591
Size: 43 15 533
Fragments arranged by size:
533 43 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 82 84 409 562 591
Size: 58 24 2 325 153 29
Fragments arranged by size:
325 153 58 29 24 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 523 591
Size: 523 68
EcoO109I rG'GnC_Cy
Cuts at: 0 7 591
Size: 7 584
HaeII r_GCGC'y
Cuts at: 0 553 590 591
Size: 553 37 1
Fragments arranged by size:
553 37 1
KasI G'GCGC_C
Cuts at: 0 586 591
Size: 586 5
MslI CAynn'nnrTG
Cuts at: 0 292 361 591
Size: 292 69 230
Fragments arranged by size:
292 230 69
MspA1I CmG'CkG
Cuts at: 0 86 396 591
Size: 86 310 195
Fragments arranged by size:
310 195 86
NaeI GCC'GGC
Cuts at: 0 201 591
Size: 201 390
NarI GG'CG_CC
Cuts at: 0 587 591
Size: 587 4
NgoMIV G'CCGG_C
Cuts at: 0 199 591
Size: 199 392
NspI r_CATG'y
Cuts at: 0 220 591
Size: 220 371
PfoI T'CCnGG_A
Cuts at: 0 74 591
Size: 74 517
PmlI CAC'GTG
Cuts at: 0 384 591
Size: 384 207
PspOMI G'GGCC_C
Cuts at: 0 6 591
Size: 6 585
PstI C_TGCA'G
Cuts at: 0 314 591
Size: 314 277
SapI GCTCTTCn'nnn_
Cuts at: 0 43 591
Size: 43 548
SbfI CC_TGCA'GG
Cuts at: 0 314 591
Size: 314 277
SfcI C'TryA_G
Cuts at: 0 310 591
Size: 310 281
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 274 591
Size: 274 317
SfoI GGC'GCC
Cuts at: 0 588 591
Size: 588 3
SphI G_CATG'C
Cuts at: 0 220 591
Size: 220 371
StuI AGG'CCT
Cuts at: 0 15 529 591
Size: 15 514 62
Fragments arranged by size:
514 62 15
Enzymes that do cut:
AcuI AlfI AlwNI ApaI AvaI BanI BanII BbeI
BglI BlpI Bme1580I BmgBI BplI BpmI BsaAI BsaHI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI
BssSI BstAPI BstXI BtsI EarI Eco57MI EcoNI EcoO109I
HaeII KasI MslI MspA1I NaeI NarI NgoMIV NspI
PfoI PmlI PspOMI PstI SapI SbfI SfcI SfiI
SfoI SphI StuI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BmrI BmtI Bpu10I BpuEI BsaI
BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssHII BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI ScaI SexAI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI