Restriction map for
pF1KE2678
(Linear) (Six-base) MAPSORT of: KIEE2678.seq Check: 7201 from: 1 to: 4452
KIEE2678.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AatII G_ACGT'C
Cuts at: 0 3820 4452
Size: 3820 632
AccI GT'mk_AC
Cuts at: 0 1568 3446 4452
Size: 1568 1878 1006
Fragments arranged by size:
1878 1568 1006
Acc65I G'GTAC_C
Cuts at: 0 192 3576 4452
Size: 192 3384 876
Fragments arranged by size:
3384 876 192
AclI AA'CG_TT
Cuts at: 0 2905 4452
Size: 2905 1547
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1035 1214 1722 2859 2944 3286 3351 4200
Size: 1035 179 508 1137 85 342 65 849
Cuts at: 4200 4452
Size: 252
Fragments arranged by size:
1137 1035 849 508 342 252 179 85
65
AfeI AGC'GCT
Cuts at: 0 1665 3361 4452
Size: 1665 1696 1091
Fragments arranged by size:
1696 1665 1091
AflII C'TTAA_G
Cuts at: 0 1393 4452
Size: 1393 3059
AflIII A'CryG_T
Cuts at: 0 617 761 2468 2936 4013 4452
Size: 617 144 1707 468 1077 439
Fragments arranged by size:
1707 1077 617 468 439 144
AgeI A'CCGG_T
Cuts at: 0 3623 4452
Size: 3623 829
AhdI GACnn_n'nnGTC
Cuts at: 0 921 4452
Size: 921 3531
AleI CACnn'nnGTG
Cuts at: 0 3682 3919 4452
Size: 3682 237 533
Fragments arranged by size:
3682 533 237
AlwNI CAG_nnn'CTG
Cuts at: 0 808 1192 3786 4452
Size: 808 384 2594 666
Fragments arranged by size:
2594 808 666 384
ApaI G_GGCC'C
Cuts at: 0 3515 4269 4452
Size: 3515 754 183
Fragments arranged by size:
3515 754 183
ApoI r'AATT_y
Cuts at: 0 1041 1400 3121 4452
Size: 1041 359 1721 1331
Fragments arranged by size:
1721 1331 1041 359
AvaI C'yCGr_G
Cuts at: 0 197 3699 4452
Size: 197 3502 753
Fragments arranged by size:
3502 753 197
BanI G'GyrC_C
Cuts at: 0 192 1239 1760 3576 4452
Size: 192 1047 521 1816 876
Fragments arranged by size:
1816 1047 876 521 192
BanII G_rGCy'C
Cuts at: 0 78 94 388 830 950 1534 3008 3515
Size: 78 16 294 442 120 584 1474 507
Cuts at: 3515 3853 4003 4269 4452
Size: 338 150 266 183
Fragments arranged by size:
1474 584 507 442 338 294 266 183
150 120 78 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 2454 2574 4452
Size: 2454 120 1878
Fragments arranged by size:
2454 1878 120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3208 3242 4452
Size: 3208 34 1210
Fragments arranged by size:
3208 1210 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 786 4452
Size: 786 3666
BclI T'GATC_A
Cuts at: 0 1016 4452
Size: 1016 3436
BfrBI ATG'CAT
Cuts at: 0 2106 2149 4452
Size: 2106 43 2303
Fragments arranged by size:
2303 2106 43
BglI GCCn_nnn'nGGC
Cuts at: 0 55 1522 3808 3949 4452
Size: 55 1467 2286 141 503
Fragments arranged by size:
2286 1467 503 141 55
BlpI GC'TnA_GC
Cuts at: 0 4117 4452
Size: 4117 335
Bme1580I G_kGCm'C
Cuts at: 0 130 776 1583 2060 3515 4070 4269 4452
Size: 130 646 807 477 1455 555 199 183
Fragments arranged by size:
1455 807 646 555 477 199 183 130
BmrI ACTGGGnnnn_n'
Cuts at: 0 770 833 3033 3444 3799 4452
Size: 770 63 2200 411 355 653
Fragments arranged by size:
2200 770 653 411 355 63
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3789 4452
Size: 3789 663
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1252 4386 4452
Size: 1252 3134 66
Fragments arranged by size:
3134 1252 66
Bpu10I CC'TnA_GC
Cuts at: 0 944 1996 4452
Size: 944 1052 2456
Fragments arranged by size:
2456 1052 944
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 4452
Size: 643 3809
BsaI GGTCTCn'nnnn_
Cuts at: 0 217 2010 3533 4296 4452
Size: 217 1793 1523 763 156
Fragments arranged by size:
1793 1523 763 217 156
BsaAI yAC'GTr
Cuts at: 0 764 3444 4452
Size: 764 2680 1008
Fragments arranged by size:
2680 1008 764
BsaBI GATnn'nnATC
Cuts at: 0 1211 4452
Size: 1211 3241
BsaHI Gr'CG_yC
Cuts at: 0 3817 4240 4452
Size: 3817 423 212
Fragments arranged by size:
3817 423 212
BsaWI w'CCGG_w
Cuts at: 0 272 3623 3719 3881 4452
Size: 272 3351 96 162 571
Fragments arranged by size:
3351 571 272 162 96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 169 199 370 400 1099 1129 1487 1517
Size: 169 30 171 30 699 30 358 30
Cuts at: 1517 2626 2656 2668 2698 3610 3640 3658 3688
Size: 1109 30 12 30 912 30 18 30
Cuts at: 3688 4452
Size: 764
Fragments arranged by size:
1109 912 764 699 358 171 169 30
30 30 30 30 30 30 30 18
12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 378 613 1249 1357 2647 2878 3075 3521
Size: 378 235 636 108 1290 231 197 446
Cuts at: 3521 4452
Size: 931
Fragments arranged by size:
1290 931 636 446 378 235 231 197
108
BseYI C'CCAG_C
Cuts at: 0 97 587 4452
Size: 97 490 3865
Fragments arranged by size:
3865 490 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1879 3566 3649 4452
Size: 1879 1687 83 803
Fragments arranged by size:
1879 1687 803 83
BsiEI CG_ry'CG
Cuts at: 0 1558 2630 3141 3337 4452
Size: 1558 1072 511 196 1115
Fragments arranged by size:
1558 1115 1072 511 196
BsiHKAI G_wGCw'C
Cuts at: 0 78 932 2810 3443 4042 4452
Size: 78 854 1878 633 599 410
Fragments arranged by size:
1878 854 633 599 410 78
BsmI GAATG_Cn'
Cuts at: 0 2826 4452
Size: 2826 1626
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1710 4452
Size: 1710 2742
Bsp1286I G_dGCh'C
Cuts at: 0 78 94 130 388 776 830 932 950
Size: 78 16 36 258 388 54 102 18
Cuts at: 950 1534 1583 2060 2810 3008 3443 3515 3853
Size: 584 49 477 750 198 435 72 338
Cuts at: 3853 4003 4042 4070 4269 4452
Size: 150 39 28 199 183
Fragments arranged by size:
750 584 477 435 388 338 258 199
198 183 150 102 78 72 54 49
39 36 28 18 16
BspHI T'CATG_A
Cuts at: 0 1505 1958 2423 4452
Size: 1505 453 465 2029
Fragments arranged by size:
2029 1505 465 453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1291 2924 3588 3935 4452
Size: 1291 1633 664 347 517
Fragments arranged by size:
1633 1291 664 517 347
BsrBI CCG'CTC
Cuts at: 0 13 3170 3344 3471 4452
Size: 13 3157 174 127 981
Fragments arranged by size:
3157 981 174 127 13
BsrDI GCAATG_nn'
Cuts at: 0 107 1629 2149 2288 2541 3231 4413 4452
Size: 107 1522 520 139 253 690 1182 39
Fragments arranged by size:
1522 1182 690 520 253 139 107 39
BsrFI r'CCGG_y
Cuts at: 0 2966 3623 3795 3896 4284 4452
Size: 2966 657 172 101 388 168
Fragments arranged by size:
2966 657 388 172 168 101
BssSI C'ACGA_G
Cuts at: 0 121 160 973 4452
Size: 121 39 813 3479
Fragments arranged by size:
3479 813 121 39
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3641 4452
Size: 3641 811
BstEII G'GTnAC_C
Cuts at: 0 1440 2244 4452
Size: 1440 804 2208
Fragments arranged by size:
2208 1440 804
BstXI CCAn_nnnn'nTGG
Cuts at: 0 783 1890 1990 2721 2980 4452
Size: 783 1107 100 731 259 1472
Fragments arranged by size:
1472 1107 783 731 259 100
BstYI r'GATC_y
Cuts at: 0 278 329 554 644 4452
Size: 278 51 225 90 3808
Fragments arranged by size:
3808 278 225 90 51
BtgI C'CryG_G
Cuts at: 0 188 378 1712 2483 2616 4194 4226 4452
Size: 188 190 1334 771 133 1578 32 226
Fragments arranged by size:
1578 1334 771 226 190 188 133 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 306 4452
Size: 306 4146
BtsI GCAGTG_nn'
Cuts at: 0 902 1532 1937 2207 2262 2660 2991 4070
Size: 902 630 405 270 55 398 331 1079
Cuts at: 4070 4452
Size: 382
Fragments arranged by size:
1079 902 630 405 398 382 331 270
55
ClaI AT'CG_AT
Cuts at: 0 2999 4452
Size: 2999 1453
DraIII CAC_nnn'GTG
Cuts at: 0 3564 4452
Size: 3564 888
EaeI y'GGCC_r
Cuts at: 0 47 56 143 728 1928 2480 2711 3150
Size: 47 9 87 585 1200 552 231 439
Cuts at: 3150 3284 4452
Size: 134 1168
Fragments arranged by size:
1200 1168 585 552 439 231 134 87
47 9
EarI CTCTTCn'nnn_
Cuts at: 0 82 973 1190 1504 2878 4452
Size: 82 891 217 314 1374 1574
Fragments arranged by size:
1574 1374 891 314 217 82
EcoICRI GAG'CTC
Cuts at: 0 76 4452
Size: 76 4376
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1035 1214 1252 1722 2859 2944 3286 3351
Size: 1035 179 38 470 1137 85 342 65
Cuts at: 3351 4200 4386 4452
Size: 849 186 66
Fragments arranged by size:
1137 1035 849 470 342 186 179 85
66 65 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1234 1287 3931 4452
Size: 1234 53 2644 521
Fragments arranged by size:
2644 1234 521 53
EcoO109I rG'GnC_Cy
Cuts at: 0 603 1514 3511 3512 4266 4452
Size: 603 911 1997 1 754 186
Fragments arranged by size:
1997 911 754 603 186 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3381 4452
Size: 3381 1071
FspI TGC'GCA
Cuts at: 0 2707 4452
Size: 2707 1745
HaeII r_GCGC'y
Cuts at: 0 1667 2322 2640 2762 3363 4452
Size: 1667 655 318 122 601 1089
Fragments arranged by size:
1667 1089 655 601 318 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 250 282 403 435 636 668 907 939
Size: 250 32 121 32 201 32 239 32
Cuts at: 939 1487 1519 1540 1572 2626 2658 2824 2856
Size: 548 32 21 32 1054 32 166 32
Cuts at: 2856 3610 3642 4452
Size: 754 32 810
Fragments arranged by size:
1054 810 754 548 250 239 201 166
121 32 32 32 32 32 32 32
32 32 21
HincII GTy'rAC
Cuts at: 0 1569 4452
Size: 1569 2883
HindIII A'AGCT_T
Cuts at: 0 458 4452
Size: 458 3994
KpnI G_GTAC'C
Cuts at: 0 196 3580 4452
Size: 196 3384 872
Fragments arranged by size:
3384 872 196
MfeI C'AATT_G
Cuts at: 0 446 4452
Size: 446 4006
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 2562 2711 2799 4379 4452
Size: 158 2404 149 88 1580 73
Fragments arranged by size:
2404 1580 158 149 88 73
MscI TGG'CCA
Cuts at: 0 145 730 1930 3152 4452
Size: 145 585 1200 1222 1300
Fragments arranged by size:
1300 1222 1200 585 145
MslI CAynn'nnrTG
Cuts at: 0 121 1525 3682 3919 4418 4452
Size: 121 1404 2157 237 499 34
Fragments arranged by size:
2157 1404 499 237 121 34
MspA1I CmG'CkG
Cuts at: 0 2436 2609 2922 3503 3572 3650 4030 4452
Size: 2436 173 313 581 69 78 380 422
Fragments arranged by size:
2436 581 422 380 313 173 78 69
NaeI GCC'GGC
Cuts at: 0 3797 3898 4452
Size: 3797 101 554
Fragments arranged by size:
3797 554 101
NcoI C'CATG_G
Cuts at: 0 2483 2616 4194 4452
Size: 2483 133 1578 258
Fragments arranged by size:
2483 1578 258 133
NdeI CA'TA_TG
Cuts at: 0 843 4452
Size: 843 3609
NgoMIV G'CCGG_C
Cuts at: 0 3795 3896 4452
Size: 3795 101 556
Fragments arranged by size:
3795 556 101
NsiI A_TGCA'T
Cuts at: 0 2108 2151 4452
Size: 2108 43 2301
Fragments arranged by size:
2301 2108 43
NspI r_CATG'y
Cuts at: 0 621 921 2110 2472 4017 4116 4417 4452
Size: 621 300 1189 362 1545 99 301 35
Fragments arranged by size:
1545 1189 621 362 301 300 99 35
PasI CC'CwG_GG
Cuts at: 0 4385 4452
Size: 4385 67
PciI A'CATG_T
Cuts at: 0 617 2468 4013 4452
Size: 617 1851 1545 439
Fragments arranged by size:
1851 1545 617 439
PflMI CCAn_nnn'nTGG
Cuts at: 0 35 288 2622 4452
Size: 35 253 2334 1830
Fragments arranged by size:
2334 1830 253 35
PmlI CAC'GTG
Cuts at: 0 764 4452
Size: 764 3688
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2890 2922 4452
Size: 2890 32 1530
Fragments arranged by size:
2890 1530 32
PpuMI rG'GwC_Cy
Cuts at: 0 603 4452
Size: 603 3849
PshAI GACnn'nnGTC
Cuts at: 0 2781 4452
Size: 2781 1671
PsiI TTA'TAA
Cuts at: 0 1754 4452
Size: 1754 2698
PspOMI G'GGCC_C
Cuts at: 0 3511 4265 4452
Size: 3511 754 187
Fragments arranged by size:
3511 754 187
PstI C_TGCA'G
Cuts at: 0 1154 2345 2921 3782 3932 4452
Size: 1154 1191 576 861 150 520
Fragments arranged by size:
1191 1154 861 576 520 150
PvuI CG_AT'CG
Cuts at: 0 1558 2630 4452
Size: 1558 1072 1822
Fragments arranged by size:
1822 1558 1072
PvuII CAG'CTG
Cuts at: 0 2436 2609 2922 3650 4030 4452
Size: 2436 173 313 728 380 422
Fragments arranged by size:
2436 728 422 380 313 173
RsrII CG'GwC_CG
Cuts at: 0 3626 4452
Size: 3626 826
SacI G_AGCT'C
Cuts at: 0 78 4452
Size: 78 4374
SalI G'TCGA_C
Cuts at: 0 1567 4452
Size: 1567 2885
SapI GCTCTTCn'nnn_
Cuts at: 0 82 1190 4452
Size: 82 1108 3262
Fragments arranged by size:
3262 1108 82
SbfI CC_TGCA'GG
Cuts at: 0 3782 4452
Size: 3782 670
ScaI AGT'ACT
Cuts at: 0 1162 1735 4452
Size: 1162 573 2717
Fragments arranged by size:
2717 1162 573
SexAI A'CCwGG_T
Cuts at: 0 1436 1982 4452
Size: 1436 546 2470
Fragments arranged by size:
2470 1436 546
SfcI C'TryA_G
Cuts at: 0 1150 2341 2604 2917 3144 3778 3928 4452
Size: 1150 1191 263 313 227 634 150 524
Fragments arranged by size:
1191 1150 634 524 313 263 227 150
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 55 4452
Size: 55 4397
SmaI CCC'GGG
Cuts at: 0 199 4452
Size: 199 4253
SmlI C'TyrA_G
Cuts at: 0 658 1393 4452
Size: 658 735 3059
Fragments arranged by size:
3059 735 658
SphI G_CATG'C
Cuts at: 0 2110 4452
Size: 2110 2342
SspI AAT'ATT
Cuts at: 0 2082 4452
Size: 2082 2370
StyI C'CwwG_G
Cuts at: 0 698 2483 2616 3586 3863 4194 4452
Size: 698 1785 133 970 277 331 258
Fragments arranged by size:
1785 970 698 331 277 258 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 241 985 1665 2300 2542 3052 3469 4452
Size: 241 744 680 635 242 510 417 983
Fragments arranged by size:
983 744 680 635 510 417 242 241
TatI w'GTAC_w
Cuts at: 0 1160 1733 4452
Size: 1160 573 2719
Fragments arranged by size:
2719 1160 573
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 169 201 370 402 1099 1131 2024 2056
Size: 169 32 169 32 697 32 893 32
Cuts at: 2056 3658 3690 4168 4200 4452
Size: 1602 32 478 32 252
Fragments arranged by size:
1602 893 697 478 252 169 169 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3496 4141 4452
Size: 3496 645 311
Fragments arranged by size:
3496 645 311
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 36 2305 4284 4452
Size: 36 2269 1979 168
Fragments arranged by size:
2269 1979 168 36
XmaI C'CCGG_G
Cuts at: 0 197 4452
Size: 197 4255
XmnI GAAnn'nnTTC
Cuts at: 0 2872 4452
Size: 2872 1580
ZraI GAC'GTC
Cuts at: 0 3818 4452
Size: 3818 634
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlwNI ApaI ApoI AvaI BanI
BanII BbsI BcgI BciVI BclI BfrBI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI DraIII EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI HaeII Hin4I HincII HindIII KpnI
MfeI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NsiI NspI PasI PciI PflMI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SmaI SmlI SphI SspI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BglII BmgBI BmtI BsiWI
BspEI BsrGI BssHII BstBI BstZ17I Bsu36I CspCI DraI
DrdI EagI EciI EcoRI EcoRV FseI FspAI HpaI
KasI MluI NarI NheI NotI NruI PacI PfoI
PmeI PspXI PsrI SacII SanDI SfoI SgfI SgrAI
SnaBI SpeI SrfI StuI SwaI XbaI XhoI