Restriction map for
pF1KE2677
(Linear) (Six-base) MAPSORT of: KIEE2677.seq Check: 7423 from: 1 to: 5460
KIEE2677.orf
With 182 enzymes: SgfI *
March 1, 2017 16:47 ..
AccI GT'mk_AC
Cuts at: 0 562 5460
Size: 562 4898
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 1646 2018 2500 2594 3607 3817 5460
Size: 59 1587 372 482 94 1013 210 1643
Fragments arranged by size:
1643 1587 1013 482 372 210 94 59
AfeI AGC'GCT
Cuts at: 0 3443 5460
Size: 3443 2017
AflIII A'CryG_T
Cuts at: 0 2841 5460
Size: 2841 2619
AleI CACnn'nnGTG
Cuts at: 0 1010 4306 4933 5460
Size: 1010 3296 627 527
Fragments arranged by size:
3296 1010 627 527
AlwNI CAG_nnn'CTG
Cuts at: 0 3369 3819 4729 5460
Size: 3369 450 910 731
Fragments arranged by size:
3369 910 731 450
ApoI r'AATT_y
Cuts at: 0 84 118 334 379 802 830 877 1346
Size: 84 34 216 45 423 28 47 469
Cuts at: 1346 1455 1792 1799 2344 2775 2804 3128 3150
Size: 109 337 7 545 431 29 324 22
Cuts at: 3150 3597 3607 4769 5041 5064 5194 5351 5460
Size: 447 10 1162 272 23 130 157 109
Fragments arranged by size:
1162 545 469 447 431 423 337 324
272 216 157 130 109 109 84 47
45 34 29 28 23 22 10 7
AseI AT'TA_AT
Cuts at: 0 2191 2950 3959 5460
Size: 2191 759 1009 1501
Fragments arranged by size:
2191 1501 1009 759
AvaI C'yCGr_G
Cuts at: 0 1952 5460
Size: 1952 3508
BanII G_rGCy'C
Cuts at: 0 1842 5269 5460
Size: 1842 3427 191
Fragments arranged by size:
3427 1842 191
BbsI GAAGACnn'nnnn_
Cuts at: 0 178 538 1171 1598 1748 2582 2630 4130
Size: 178 360 633 427 150 834 48 1500
Cuts at: 4130 5460
Size: 1330
Fragments arranged by size:
1500 1330 834 633 427 360 178 150
48
BbvCI CC'TCA_GC
Cuts at: 0 4463 5460
Size: 4463 997
BclI T'GATC_A
Cuts at: 0 2319 4836 5415 5460
Size: 2319 2517 579 45
Fragments arranged by size:
2517 2319 579 45
BfrBI ATG'CAT
Cuts at: 0 287 5460
Size: 287 5173
BglII A'GATC_T
Cuts at: 0 939 3828 4095 5460
Size: 939 2889 267 1365
Fragments arranged by size:
2889 1365 939 267
BmrI ACTGGGnnnn_n'
Cuts at: 0 1208 5460
Size: 1208 4252
BmtI G_CTAG'C
Cuts at: 0 644 5460
Size: 644 4816
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3807 5460
Size: 3807 1653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 3988 5460
Size: 160 3828 1472
Fragments arranged by size:
3828 1472 160
Bpu10I CC'TnA_GC
Cuts at: 0 315 4463 5460
Size: 315 4148 997
Fragments arranged by size:
4148 997 315
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 1448 1481 2275 3652 4015 4204 4397
Size: 244 1204 33 794 1377 363 189 193
Cuts at: 4397 5460
Size: 1063
Fragments arranged by size:
1377 1204 1063 794 363 244 193 189
33
BsaAI yAC'GTr
Cuts at: 0 2977 5460
Size: 2977 2483
BsaBI GATnn'nnATC
Cuts at: 0 4405 5460
Size: 4405 1055
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1624 1726 3064 4999 5460
Size: 1624 102 1338 1935 461
Fragments arranged by size:
1935 1624 1338 461 102
BseYI C'CCAG_C
Cuts at: 0 360 4517 5460
Size: 360 4157 943
Fragments arranged by size:
4157 943 360
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2866 5460
Size: 2866 2594
BsiEI CG_ry'CG
Cuts at: 0 2605 5460
Size: 2605 2855
BsiHKAI G_wGCw'C
Cuts at: 0 5269 5460
Size: 5269 191
BsmI GAATG_Cn'
Cuts at: 0 509 5022 5460
Size: 509 4513 438
Fragments arranged by size:
4513 509 438
Bsp1286I G_dGCh'C
Cuts at: 0 1842 5269 5460
Size: 1842 3427 191
Fragments arranged by size:
3427 1842 191
BspHI T'CATG_A
Cuts at: 0 114 2882 5460
Size: 114 2768 2578
Fragments arranged by size:
2768 2578 114
BsrDI GCAATG_nn'
Cuts at: 0 652 1421 2394 2609 4254 5460
Size: 652 769 973 215 1645 1206
Fragments arranged by size:
1645 1206 973 769 652 215
BsrGI T'GTAC_A
Cuts at: 0 4620 5460
Size: 4620 840
BssSI C'ACGA_G
Cuts at: 0 1103 1708 5460
Size: 1103 605 3752
Fragments arranged by size:
3752 1103 605
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3802 5460
Size: 3802 1658
BstBI TT'CG_AA
Cuts at: 0 158 189 5442 5460
Size: 158 31 5253 18
Fragments arranged by size:
5253 158 31 18
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4224 5460
Size: 4224 1236
BstYI r'GATC_y
Cuts at: 0 589 939 3030 3828 4095 4725 4795 5460
Size: 589 350 2091 798 267 630 70 665
Fragments arranged by size:
2091 798 665 630 589 350 267 70
BstZ17I GTA'TAC
Cuts at: 0 563 5460
Size: 563 4897
Bsu36I CC'TnA_GG
Cuts at: 0 2459 5460
Size: 2459 3001
BtsI GCAGTG_nn'
Cuts at: 0 4913 5460
Size: 4913 547
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1971 2006 5460
Size: 1971 35 3454
Fragments arranged by size:
3454 1971 35
DraI TTT'AAA
Cuts at: 0 989 1454 1797 2382 3653 5460
Size: 989 465 343 585 1271 1807
Fragments arranged by size:
1807 1271 989 585 465 343
DraIII CAC_nnn'GTG
Cuts at: 0 3463 5460
Size: 3463 1997
DrdI GACnn_nn'nnGTC
Cuts at: 0 434 5460
Size: 434 5026
EaeI y'GGCC_r
Cuts at: 0 4214 5162 5460
Size: 4214 948 298
Fragments arranged by size:
4214 948 298
EarI CTCTTCn'nnn_
Cuts at: 0 2048 2111 2201 3216 4957 4963 5274 5460
Size: 2048 63 90 1015 1741 6 311 186
Fragments arranged by size:
2048 1741 1015 311 186 90 63 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1407 5460
Size: 1407 4053
EcoICRI GAG'CTC
Cuts at: 0 5267 5460
Size: 5267 193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 160 1646 2018 2500 2594 3607 3817
Size: 59 101 1486 372 482 94 1013 210
Cuts at: 3817 3988 5460
Size: 171 1472
Fragments arranged by size:
1486 1472 1013 482 372 210 171 101
94 59
EcoRI G'AATT_C
Cuts at: 0 379 3128 5460
Size: 379 2749 2332
Fragments arranged by size:
2749 2332 379
EcoRV GAT'ATC
Cuts at: 0 4610 4698 5460
Size: 4610 88 762
Fragments arranged by size:
4610 762 88
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 399 3373 3637 3847 5460
Size: 399 2974 264 210 1613
Fragments arranged by size:
2974 1613 399 264 210
HaeII r_GCGC'y
Cuts at: 0 3445 5460
Size: 3445 2015
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2177 2209 2455 2487 4780 4787 4812 4819
Size: 2177 32 246 32 2293 7 25 7
Cuts at: 4819 5460
Size: 641
Fragments arranged by size:
2293 2177 641 246 32 32 25 7
7
HincII GTy'rAC
Cuts at: 0 3695 4826 5460
Size: 3695 1131 634
Fragments arranged by size:
3695 1131 634
HindIII A'AGCT_T
Cuts at: 0 100 5460
Size: 100 5360
HpaI GTT'AAC
Cuts at: 0 3695 5460
Size: 3695 1765
MfeI C'AATT_G
Cuts at: 0 62 2828 5460
Size: 62 2766 2632
Fragments arranged by size:
2766 2632 62
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1709 2823 5460
Size: 1709 1114 2637
Fragments arranged by size:
2637 1709 1114
MscI TGG'CCA
Cuts at: 0 4216 5164 5460
Size: 4216 948 296
Fragments arranged by size:
4216 948 296
MslI CAynn'nnrTG
Cuts at: 0 1010 2012 2382 4132 4306 4933 5460
Size: 1010 1002 370 1750 174 627 527
Fragments arranged by size:
1750 1010 1002 627 527 370 174
MspA1I CmG'CkG
Cuts at: 0 3369 4521 5460
Size: 3369 1152 939
Fragments arranged by size:
3369 1152 939
NdeI CA'TA_TG
Cuts at: 0 2366 5460
Size: 2366 3094
NheI G'CTAG_C
Cuts at: 0 640 5460
Size: 640 4820
NsiI A_TGCA'T
Cuts at: 0 289 5460
Size: 289 5171
NspI r_CATG'y
Cuts at: 0 1285 2845 3424 4091 4131 5460
Size: 1285 1560 579 667 40 1329
Fragments arranged by size:
1560 1329 1285 667 579 40
PciI A'CATG_T
Cuts at: 0 2841 5460
Size: 2841 2619
PflMI CCAn_nnn'nTGG
Cuts at: 0 1562 5460
Size: 1562 3898
PfoI T'CCnGG_A
Cuts at: 0 1292 5460
Size: 1292 4168
PsiI TTA'TAA
Cuts at: 0 934 5460
Size: 934 4526
PvuII CAG'CTG
Cuts at: 0 3369 4521 5460
Size: 3369 1152 939
Fragments arranged by size:
3369 1152 939
SacI G_AGCT'C
Cuts at: 0 5269 5460
Size: 5269 191
SapI GCTCTTCn'nnn_
Cuts at: 0 5274 5460
Size: 5274 186
SfcI C'TryA_G
Cuts at: 0 978 4799 5188 5460
Size: 978 3821 389 272
Fragments arranged by size:
3821 978 389 272
SmlI C'TyrA_G
Cuts at: 0 223 1463 1496 1952 2254 3667 4030 4183
Size: 223 1240 33 456 302 1413 363 153
Cuts at: 4183 4412 5460
Size: 229 1048
Fragments arranged by size:
1413 1240 1048 456 363 302 229 223
153 33
SnaBI TAC'GTA
Cuts at: 0 2977 5460
Size: 2977 2483
SphI G_CATG'C
Cuts at: 0 4091 5460
Size: 4091 1369
SspI AAT'ATT
Cuts at: 0 504 1076 1535 5460
Size: 504 572 459 3925
Fragments arranged by size:
3925 572 504 459
StuI AGG'CCT
Cuts at: 0 72 3208 5460
Size: 72 3136 2252
Fragments arranged by size:
3136 2252 72
StyI C'CwwG_G
Cuts at: 0 291 347 1787 4952 5460
Size: 291 56 1440 3165 508
Fragments arranged by size:
3165 1440 508 291 56
SwaI ATTT'AAAT
Cuts at: 0 2382 3653 5460
Size: 2382 1271 1807
Fragments arranged by size:
2382 1807 1271
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2619 5040 5460
Size: 2619 2421 420
Fragments arranged by size:
2619 2421 420
TatI w'GTAC_w
Cuts at: 0 3903 4620 5460
Size: 3903 717 840
Fragments arranged by size:
3903 840 717
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4836 4868 5460
Size: 4836 32 592
Fragments arranged by size:
4836 592 32
Tth111I GACn'n_nGTC
Cuts at: 0 431 5460
Size: 431 5029
XbaI T'CTAG_A
Cuts at: 0 1575 5460
Size: 1575 3885
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 783 5460
Size: 783 4677
XhoI C'TCGA_G
Cuts at: 0 1952 5460
Size: 1952 3508
XmnI GAAnn'nnTTC
Cuts at: 0 379 383 3620 5067 5460
Size: 379 4 3237 1447 393
Fragments arranged by size:
3237 1447 393 379 4
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI AlwNI ApoI AseI
AvaI BanII BbsI BbvCI BclI BfrBI BglII BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrDI
BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I
BtsI CspCI DraI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoRI EcoRV FalI HaeII Hin4I HincII
HindIII HpaI MfeI MmeI MscI MslI MspA1I NdeI
NheI NsiI NspI PciI PflMI PfoI PsiI PvuII
SacI SapI SfcI SmlI SnaBI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BanI BbeI BcgI BciVI BglI BlpI Bme1580I BmgBI
BsaI BsaHI BsaWI BsaXI BsiWI BsmBI BspEI BspMI
BsrBI BsrFI BssHII BstEII BtgI BtgZI ClaI EagI
EcoNI EcoO109I FseI FspI FspAI KasI KpnI MluI
NaeI NarI NcoI NgoMIV NotI NruI PacI PasI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SrfI
XmaI ZraI