Restriction map for
pF1KE2672
(Linear) (Six-base) MAPSORT of: KIEE2672.seq Check: 1093 from: 1 to: 2295
KIEE2672.orf
With 182 enzymes: SgfI *
February 10, 2017 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 812 2295
Size: 812 1483
Acc65I G'GTAC_C
Cuts at: 0 1473 2295
Size: 1473 822
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1222 1326 1619 2121 2295
Size: 1222 104 293 502 174
Fragments arranged by size:
1222 502 293 174 104
AfeI AGC'GCT
Cuts at: 0 365 2295
Size: 365 1930
AleI CACnn'nnGTG
Cuts at: 0 1921 2295
Size: 1921 374
AlwNI CAG_nnn'CTG
Cuts at: 0 77 231 300 568 813 894 993 1041
Size: 77 154 69 268 245 81 99 48
Cuts at: 1041 2295
Size: 1254
Fragments arranged by size:
1254 268 245 154 99 81 77 69
48
ApaI G_GGCC'C
Cuts at: 0 210 693 1390 1905 2295
Size: 210 483 697 515 390
Fragments arranged by size:
697 515 483 390 210
ApaLI G'TGCA_C
Cuts at: 0 334 2295
Size: 334 1961
AvaI C'yCGr_G
Cuts at: 0 1205 1526 1669 2078 2295
Size: 1205 321 143 409 217
Fragments arranged by size:
1205 409 321 217 143
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1465 1498 2295
Size: 1465 33 797
Fragments arranged by size:
1465 797 33
BamHI G'GATC_C
Cuts at: 0 2048 2295
Size: 2048 247
BanI G'GyrC_C
Cuts at: 0 1117 1473 2295
Size: 1117 356 822
Fragments arranged by size:
1117 822 356
BanII G_rGCy'C
Cuts at: 0 210 446 461 525 693 1204 1390 1905
Size: 210 236 15 64 168 511 186 515
Cuts at: 1905 2086 2295
Size: 181 209
Fragments arranged by size:
515 511 236 210 209 186 181 168
64 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 2027 2295
Size: 2027 268
BglI GCCn_nnn'nGGC
Cuts at: 0 538 2295
Size: 538 1757
BglII A'GATC_T
Cuts at: 0 2031 2295
Size: 2031 264
Bme1580I G_kGCm'C
Cuts at: 0 210 338 693 827 1390 1905 2295
Size: 210 128 355 134 563 515 390
Fragments arranged by size:
563 515 390 355 210 134 128
BmrI ACTGGGnnnn_n'
Cuts at: 0 912 2295
Size: 912 1383
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 987 2295
Size: 987 1308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 718 881 1951 2284 2295
Size: 415 303 163 1070 333 11
Fragments arranged by size:
1070 415 333 303 163 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 1060 1159 2295
Size: 298 762 99 1136
Fragments arranged by size:
1136 762 298 99
BsaI GGTCTCn'nnnn_
Cuts at: 0 844 1991 2228 2295
Size: 844 1147 237 67
Fragments arranged by size:
1147 844 237 67
BsaHI Gr'CG_yC
Cuts at: 0 1051 2295
Size: 1051 1244
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 251 359 661 963 1687 1828 2244 2295
Size: 251 108 302 302 724 141 416 51
Fragments arranged by size:
724 416 302 302 251 141 108 51
BseYI C'CCAG_C
Cuts at: 0 1113 1497 1749 2124 2279 2295
Size: 1113 384 252 375 155 16
Fragments arranged by size:
1113 384 375 252 155 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 466 2295
Size: 461 5 1829
Fragments arranged by size:
1829 461 5
BsiEI CG_ry'CG
Cuts at: 0 1099 2295
Size: 1099 1196
BsiHKAI G_wGCw'C
Cuts at: 0 338 461 614 2295
Size: 338 123 153 1681
Fragments arranged by size:
1681 338 153 123
BsmBI CGTCTCn'nnnn_
Cuts at: 0 11 186 2295
Size: 11 175 2109
Fragments arranged by size:
2109 175 11
Bsp1286I G_dGCh'C
Cuts at: 0 210 338 446 461 525 614 693 827
Size: 210 128 108 15 64 89 79 134
Cuts at: 827 1204 1390 1905 2086 2295
Size: 377 186 515 181 209
Fragments arranged by size:
515 377 210 209 186 181 134 128
108 89 79 64 15
BspHI T'CATG_A
Cuts at: 0 1559 2295
Size: 1559 736
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 239 812 1778 2246 2295
Size: 239 573 966 468 49
Fragments arranged by size:
966 573 468 239 49
BsrBI CCG'CTC
Cuts at: 0 1884 1935 2295
Size: 1884 51 360
Fragments arranged by size:
1884 360 51
BsrDI GCAATG_nn'
Cuts at: 0 1841 2295
Size: 1841 454
BsrFI r'CCGG_y
Cuts at: 0 27 105 1053 2039 2295
Size: 27 78 948 986 256
Fragments arranged by size:
986 948 256 78 27
BsrGI T'GTAC_A
Cuts at: 0 764 1133 1781 2295
Size: 764 369 648 514
Fragments arranged by size:
764 648 514 369
BssSI C'ACGA_G
Cuts at: 0 820 1197 1492 2295
Size: 820 377 295 803
Fragments arranged by size:
820 803 377 295
BstAPI GCAn_nnn'nTGC
Cuts at: 0 176 231 897 993 2295
Size: 176 55 666 96 1302
Fragments arranged by size:
1302 666 176 96 55
BstEII G'GTnAC_C
Cuts at: 0 1431 2295
Size: 1431 864
BstYI r'GATC_y
Cuts at: 0 248 1037 1970 2031 2048 2295
Size: 248 789 933 61 17 247
Fragments arranged by size:
933 789 248 247 61 17
Bsu36I CC'TnA_GG
Cuts at: 0 89 975 1259 2227 2295
Size: 89 886 284 968 68
Fragments arranged by size:
968 886 284 89 68
BtgI C'CryG_G
Cuts at: 0 119 350 404 1436 1700 1919 2295
Size: 119 231 54 1032 264 219 376
Fragments arranged by size:
1032 376 264 231 219 119 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 471 1797 2295
Size: 471 1326 498
Fragments arranged by size:
1326 498 471
BtsI GCAGTG_nn'
Cuts at: 0 130 478 566 1819 2199 2295
Size: 130 348 88 1253 380 96
Fragments arranged by size:
1253 380 348 130 96 88
ClaI AT'CG_AT
Cuts at: 0 1742 2295
Size: 1742 553
DraIII CAC_nnn'GTG
Cuts at: 0 920 1154 2295
Size: 920 234 1141
Fragments arranged by size:
1141 920 234
EaeI y'GGCC_r
Cuts at: 0 1096 1913 2295
Size: 1096 817 382
Fragments arranged by size:
1096 817 382
EagI C'GGCC_G
Cuts at: 0 1096 2295
Size: 1096 1199
EarI CTCTTCn'nnn_
Cuts at: 0 238 2295
Size: 238 2057
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 565 2295
Size: 565 1730
EcoICRI GAG'CTC
Cuts at: 0 459 2295
Size: 459 1836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 718 881 1222 1326 1619 1951 2121
Size: 415 303 163 341 104 293 332 170
Cuts at: 2121 2284 2295
Size: 163 11
Fragments arranged by size:
415 341 332 303 293 170 163 163
104 11
EcoO109I rG'GnC_Cy
Cuts at: 0 4 93 297 1386 1546 1665 1901 2222
Size: 4 89 204 1089 160 119 236 321
Cuts at: 2222 2295
Size: 73
Fragments arranged by size:
1089 321 236 204 160 119 89 73
4
EcoRV GAT'ATC
Cuts at: 0 1269 2295
Size: 1269 1026
FseI GG_CCGG'CC
Cuts at: 0 31 2295
Size: 31 2264
HaeII r_GCGC'y
Cuts at: 0 367 2295
Size: 367 1928
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 668 700 930 962 973 1005 1361 1393
Size: 668 32 230 32 11 32 356 32
Cuts at: 1393 2089 2121 2295
Size: 696 32 174
Fragments arranged by size:
696 668 356 230 174 32 32 32
32 32 11
KpnI G_GTAC'C
Cuts at: 0 1477 2295
Size: 1477 818
MfeI C'AATT_G
Cuts at: 0 2199 2295
Size: 2199 96
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2076 2295
Size: 2076 219
MscI TGG'CCA
Cuts at: 0 1915 2295
Size: 1915 380
MslI CAynn'nnrTG
Cuts at: 0 880 1441 1558 1846 1921 1924 2295
Size: 880 561 117 288 75 3 371
Fragments arranged by size:
880 561 371 288 117 75 3
MspA1I CmG'CkG
Cuts at: 0 1463 2003 2046 2295
Size: 1463 540 43 249
Fragments arranged by size:
1463 540 249 43
NaeI GCC'GGC
Cuts at: 0 29 107 2295
Size: 29 78 2188
Fragments arranged by size:
2188 78 29
NcoI C'CATG_G
Cuts at: 0 119 350 404 1436 1700 2295
Size: 119 231 54 1032 264 595
Fragments arranged by size:
1032 595 264 231 119 54
NgoMIV G'CCGG_C
Cuts at: 0 27 105 2295
Size: 27 78 2190
Fragments arranged by size:
2190 78 27
NspI r_CATG'y
Cuts at: 0 1113 2295
Size: 1113 1182
PasI CC'CwG_GG
Cuts at: 0 702 2295
Size: 702 1593
PflMI CCAn_nnn'nTGG
Cuts at: 0 1041 1911 2295
Size: 1041 870 384
Fragments arranged by size:
1041 870 384
PfoI T'CCnGG_A
Cuts at: 0 1040 1966 2295
Size: 1040 926 329
Fragments arranged by size:
1040 926 329
PpuMI rG'GwC_Cy
Cuts at: 0 4 1665 2222 2295
Size: 4 1661 557 73
Fragments arranged by size:
1661 557 73 4
PshAI GACnn'nnGTC
Cuts at: 0 198 2295
Size: 198 2097
PspOMI G'GGCC_C
Cuts at: 0 206 689 1386 1901 2295
Size: 206 483 697 515 394
Fragments arranged by size:
697 515 483 394 206
PstI C_TGCA'G
Cuts at: 0 181 239 809 2295
Size: 181 58 570 1486
Fragments arranged by size:
1486 570 181 58
SacI G_AGCT'C
Cuts at: 0 461 2295
Size: 461 1834
SanDI GG'GwC_CC
Cuts at: 0 2222 2295
Size: 2222 73
SexAI A'CCwGG_T
Cuts at: 0 512 2295
Size: 512 1783
SfcI C'TryA_G
Cuts at: 0 10 177 235 805 2295
Size: 10 167 58 570 1490
Fragments arranged by size:
1490 570 167 58 10
SgrAI Cr'CCGG_yG
Cuts at: 0 1053 2295
Size: 1053 1242
SmaI CCC'GGG
Cuts at: 0 1207 2080 2295
Size: 1207 873 215
Fragments arranged by size:
1207 873 215
SmlI C'TyrA_G
Cuts at: 0 313 1075 1174 2295
Size: 313 762 99 1121
Fragments arranged by size:
1121 762 313 99
SphI G_CATG'C
Cuts at: 0 1113 2295
Size: 1113 1182
StuI AGG'CCT
Cuts at: 0 2068 2295
Size: 2068 227
StyI C'CwwG_G
Cuts at: 0 33 55 98 119 137 350 404 1380
Size: 33 22 43 21 18 213 54 976
Cuts at: 1380 1436 1700 2295
Size: 56 264 595
Fragments arranged by size:
976 595 264 213 56 54 43 33
22 21 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 931 1105 2295
Size: 931 174 1190
Fragments arranged by size:
1190 931 174
TatI w'GTAC_w
Cuts at: 0 764 1133 1517 1781 2295
Size: 764 369 384 264 514
Fragments arranged by size:
764 514 384 369 264
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 604 636 698 730 1879 1911 2054 2086
Size: 604 32 62 32 1149 32 143 32
Cuts at: 2086 2295
Size: 209
Fragments arranged by size:
1149 604 209 143 62 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 411 925 1467 1761 1926 2295
Size: 411 514 542 294 165 369
Fragments arranged by size:
542 514 411 369 294 165
XmaI C'CCGG_G
Cuts at: 0 1205 2078 2295
Size: 1205 873 217
Fragments arranged by size:
1205 873 217
XmnI GAAnn'nnTTC
Cuts at: 0 2210 2295
Size: 2210 85
Enzymes that do cut:
AarI Acc65I AcuI AfeI AleI AlwNI ApaI ApaLI
AvaI BaeI BamHI BanI BanII BbsI BglI BglII
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRV FseI HaeII
Hin4I KpnI MfeI MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PasI PflMI PfoI PpuMI PshAI
PspOMI PstI SacI SanDI SexAI SfcI SgrAI SmaI
SmlI SphI StuI StyI TaqII TatI TstI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AhdI AlfI
AloI ApoI AscI AseI AsiSI AvrII BbeI BbvCI
BcgI BciVI BclI BfrBI BlpI BmgBI BmtI Bpu10I
BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BspEI BssHII
BstBI BstXI BstZ17I CspCI DraI DrdI EcoNI EcoRI
FalI FspI FspAI HincII HindIII HpaI KasI MluI
NarI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PsiI PspXI PsrI PvuI PvuII
RsrII SacII SalI SapI SbfI ScaI SfiI SfoI
SgfI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI
XhoI ZraI