Restriction map for
pF1KE2665
(Linear) (Six-base) MAPSORT of: KIEE2665.seq Check: 4773 from: 1 to: 1119
KIEE2665.orf
With 182 enzymes: SgfI *
January 12, 2017 11:32 ..
AccI GT'mk_AC
Cuts at: 0 356 867 1119
Size: 356 511 252
Fragments arranged by size:
511 356 252
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 1119
Size: 340 779
AflIII A'CryG_T
Cuts at: 0 806 1119
Size: 806 313
AlwNI CAG_nnn'CTG
Cuts at: 0 249 365 915 1041 1110 1119
Size: 249 116 550 126 69 9
Fragments arranged by size:
550 249 126 116 69 9
ApoI r'AATT_y
Cuts at: 0 18 252 1119
Size: 18 234 867
Fragments arranged by size:
867 234 18
AseI AT'TA_AT
Cuts at: 0 674 1119
Size: 674 445
AvaI C'yCGr_G
Cuts at: 0 764 1119
Size: 764 355
BamHI G'GATC_C
Cuts at: 0 685 1119
Size: 685 434
BbsI GAAGACnn'nnnn_
Cuts at: 0 653 1119
Size: 653 466
BbvCI CC'TCA_GC
Cuts at: 0 4 1103 1119
Size: 4 1099 16
Fragments arranged by size:
1099 16 4
BclI T'GATC_A
Cuts at: 0 536 1119
Size: 536 583
BfrBI ATG'CAT
Cuts at: 0 47 1119
Size: 47 1072
Bme1580I G_kGCm'C
Cuts at: 0 61 1119
Size: 61 1058
Bpu10I CC'TnA_GC
Cuts at: 0 4 1103 1119
Size: 4 1099 16
Fragments arranged by size:
1099 16 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 86 752 1119
Size: 86 666 367
Fragments arranged by size:
666 367 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 763 1119
Size: 580 183 356
Fragments arranged by size:
580 356 183
Bsp1286I G_dGCh'C
Cuts at: 0 61 1119
Size: 61 1058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 923 1119
Size: 923 196
BsrDI GCAATG_nn'
Cuts at: 0 143 1119
Size: 143 976
BstYI r'GATC_y
Cuts at: 0 315 685 995 1038 1119
Size: 315 370 310 43 81
Fragments arranged by size:
370 315 310 81 43
BstZ17I GTA'TAC
Cuts at: 0 357 1119
Size: 357 762
BtsI GCAGTG_nn'
Cuts at: 0 746 1017 1119
Size: 746 271 102
Fragments arranged by size:
746 271 102
DrdI GACnn_nn'nnGTC
Cuts at: 0 207 1119
Size: 207 912
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 1119
Size: 340 779
EcoO109I rG'GnC_Cy
Cuts at: 0 912 1119
Size: 912 207
EcoRI G'AATT_C
Cuts at: 0 252 1119
Size: 252 867
HincII GTy'rAC
Cuts at: 0 1046 1119
Size: 1046 73
HindIII A'AGCT_T
Cuts at: 0 1088 1119
Size: 1088 31
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 504 791 1119
Size: 244 260 287 328
Fragments arranged by size:
328 287 260 244
NsiI A_TGCA'T
Cuts at: 0 49 1119
Size: 49 1070
NspI r_CATG'y
Cuts at: 0 810 1119
Size: 810 309
PciI A'CATG_T
Cuts at: 0 806 1119
Size: 806 313
PpuMI rG'GwC_Cy
Cuts at: 0 912 1119
Size: 912 207
PstI C_TGCA'G
Cuts at: 0 138 1119
Size: 138 981
ScaI AGT'ACT
Cuts at: 0 345 1119
Size: 345 774
SfcI C'TryA_G
Cuts at: 0 134 1119
Size: 134 985
SmlI C'TyrA_G
Cuts at: 0 764 1119
Size: 764 355
StuI AGG'CCT
Cuts at: 0 235 288 748 1119
Size: 235 53 460 371
Fragments arranged by size:
460 371 235 53
StyI C'CwwG_G
Cuts at: 0 66 1119
Size: 66 1053
TatI w'GTAC_w
Cuts at: 0 343 829 1119
Size: 343 486 290
Fragments arranged by size:
486 343 290
XbaI T'CTAG_A
Cuts at: 0 873 1119
Size: 873 246
XhoI C'TCGA_G
Cuts at: 0 764 1119
Size: 764 355
XmnI GAAnn'nnTTC
Cuts at: 0 579 1119
Size: 579 540
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApoI AseI AvaI BamHI
BbsI BbvCI BclI BfrBI Bme1580I Bpu10I BseRI BsgI
Bsp1286I BspMI BsrDI BstYI BstZ17I BtsI DrdI Eco57MI
EcoO109I EcoRI HincII HindIII MmeI NsiI NspI PciI
PpuMI PstI ScaI SfcI SmlI StuI StyI TatI
XbaI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvrII
BaeI BanI BanII BbeI BcgI BciVI BglI BglII
BlpI BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII EaeI EagI EarI
EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HpaI KasI KpnI MfeI MluI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PasI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TstI Tth111I XcmI XmaI
ZraI