Restriction map for
pF1KE2663
(Linear) (Six-base) MAPSORT of: KIEE2663.seq Check: 1774 from: 1 to: 1473
KIEE2663.orf
With 182 enzymes: SgfI *
January 20, 2017 09:25 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 947 1473
Size: 947 526
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 71 1473
Size: 71 1402
ApoI r'AATT_y
Cuts at: 0 960 1473
Size: 960 513
AvaI C'yCGr_G
Cuts at: 0 131 606 639 1473
Size: 131 475 33 834
Fragments arranged by size:
834 475 131 33
AvrII C'CTAG_G
Cuts at: 0 1258 1473
Size: 1258 215
BanI G'GyrC_C
Cuts at: 0 122 1473
Size: 122 1351
BanII G_rGCy'C
Cuts at: 0 598 908 1473
Size: 598 310 565
Fragments arranged by size:
598 565 310
BbeI G_GCGC'C
Cuts at: 0 126 1473
Size: 126 1347
BbsI GAAGACnn'nnnn_
Cuts at: 0 1143 1316 1473
Size: 1143 173 157
Fragments arranged by size:
1143 173 157
BbvCI CC'TCA_GC
Cuts at: 0 82 1473
Size: 82 1391
BclI T'GATC_A
Cuts at: 0 875 1473
Size: 875 598
BglI GCCn_nnn'nGGC
Cuts at: 0 120 1473
Size: 120 1353
BglII A'GATC_T
Cuts at: 0 1393 1473
Size: 1393 80
Bme1580I G_kGCm'C
Cuts at: 0 1337 1473
Size: 1337 136
BmrI ACTGGGnnnn_n'
Cuts at: 0 902 1473
Size: 902 571
Bpu10I CC'TnA_GC
Cuts at: 0 82 1473
Size: 82 1391
BsaI GGTCTCn'nnnn_
Cuts at: 0 454 1473
Size: 454 1019
BsaHI Gr'CG_yC
Cuts at: 0 123 1473
Size: 123 1350
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 585 615 1048 1078 1473
Size: 585 30 433 30 395
Fragments arranged by size:
585 433 395 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 694 1473
Size: 694 779
BsiEI CG_ry'CG
Cuts at: 0 629 1473
Size: 629 844
BsiHKAI G_wGCw'C
Cuts at: 0 209 598 1473
Size: 209 389 875
Fragments arranged by size:
875 389 209
BsmI GAATG_Cn'
Cuts at: 0 360 1473
Size: 360 1113
BsmBI CGTCTCn'nnnn_
Cuts at: 0 95 1473
Size: 95 1378
Bsp1286I G_dGCh'C
Cuts at: 0 209 598 908 1337 1473
Size: 209 389 310 429 136
Fragments arranged by size:
429 389 310 209 136
BspHI T'CATG_A
Cuts at: 0 441 1473
Size: 441 1032
BsrDI GCAATG_nn'
Cuts at: 0 865 1473
Size: 865 608
BsrFI r'CCGG_y
Cuts at: 0 125 1473
Size: 125 1348
BsrGI T'GTAC_A
Cuts at: 0 551 1473
Size: 551 922
BstAPI GCAn_nnn'nTGC
Cuts at: 0 357 726 1473
Size: 357 369 747
Fragments arranged by size:
747 369 357
BstBI TT'CG_AA
Cuts at: 0 660 1473
Size: 660 813
BstYI r'GATC_y
Cuts at: 0 1393 1473
Size: 1393 80
BtgI C'CryG_G
Cuts at: 0 395 674 1473
Size: 395 279 799
Fragments arranged by size:
799 395 279
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 483 518 1473
Size: 483 35 955
Fragments arranged by size:
955 483 35
EaeI y'GGCC_r
Cuts at: 0 32 233 1473
Size: 32 201 1240
Fragments arranged by size:
1240 201 32
EcoICRI GAG'CTC
Cuts at: 0 596 1473
Size: 596 877
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 947 1473
Size: 947 526
EcoNI CCTnn'n_nnAGG
Cuts at: 0 42 1473
Size: 42 1431
EcoO109I rG'GnC_Cy
Cuts at: 0 1207 1473
Size: 1207 266
EcoRV GAT'ATC
Cuts at: 0 486 1473
Size: 486 987
HaeII r_GCGC'y
Cuts at: 0 126 1473
Size: 126 1347
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 194 226 1048 1080 1473
Size: 194 32 822 32 393
Fragments arranged by size:
822 393 194 32 32
HincII GTy'rAC
Cuts at: 0 78 293 1473
Size: 78 215 1180
Fragments arranged by size:
1180 215 78
HindIII A'AGCT_T
Cuts at: 0 1375 1473
Size: 1375 98
KasI G'GCGC_C
Cuts at: 0 122 1473
Size: 122 1351
MfeI C'AATT_G
Cuts at: 0 836 1473
Size: 836 637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1244 1473
Size: 1244 229
MslI CAynn'nnrTG
Cuts at: 0 475 1473
Size: 475 998
MspA1I CmG'CkG
Cuts at: 0 809 1473
Size: 809 664
NaeI GCC'GGC
Cuts at: 0 127 1473
Size: 127 1346
NarI GG'CG_CC
Cuts at: 0 123 1473
Size: 123 1350
NcoI C'CATG_G
Cuts at: 0 395 1473
Size: 395 1078
NgoMIV G'CCGG_C
Cuts at: 0 125 1473
Size: 125 1348
NspI r_CATG'y
Cuts at: 0 76 552 1473
Size: 76 476 921
Fragments arranged by size:
921 476 76
PpuMI rG'GwC_Cy
Cuts at: 0 1207 1473
Size: 1207 266
PshAI GACnn'nnGTC
Cuts at: 0 1205 1473
Size: 1205 268
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 535 567 1473
Size: 535 32 906
Fragments arranged by size:
906 535 32
PvuI CG_AT'CG
Cuts at: 0 629 1473
Size: 629 844
SacI G_AGCT'C
Cuts at: 0 598 1473
Size: 598 875
SanDI GG'GwC_CC
Cuts at: 0 1207 1473
Size: 1207 266
SfoI GGC'GCC
Cuts at: 0 124 1473
Size: 124 1349
SmaI CCC'GGG
Cuts at: 0 133 1473
Size: 133 1340
SmlI C'TyrA_G
Cuts at: 0 606 1473
Size: 606 867
StyI C'CwwG_G
Cuts at: 0 395 1036 1190 1258 1473
Size: 395 641 154 68 215
Fragments arranged by size:
641 395 215 154 68
TatI w'GTAC_w
Cuts at: 0 551 1073 1473
Size: 551 522 400
Fragments arranged by size:
551 522 400
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 585 617 1171 1203 1473
Size: 585 32 554 32 270
Fragments arranged by size:
585 554 270 32 32
XhoI C'TCGA_G
Cuts at: 0 606 1473
Size: 606 867
XmaI C'CCGG_G
Cuts at: 0 131 1473
Size: 131 1342
Enzymes that do cut:
AcuI AlfI ApoI AvaI AvrII BanI BanII BbeI
BbsI BbvCI BclI BglI BglII Bme1580I BmrI Bpu10I
BsaI BsaHI BsaXI BseRI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BsrDI BsrFI BsrGI BstAPI BstBI BstYI
BtgI CspCI EaeI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
HaeII Hin4I HincII HindIII KasI MfeI MmeI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PpuMI PshAI
PsrI PvuI SacI SanDI SfoI SmaI SmlI StyI
TatI TstI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI BaeI BamHI BcgI BciVI BfrBI BlpI
BmgBI BmtI BplI BpmI BpuEI BsaAI BsaBI BsaWI
BseYI BsgI BsiWI BspEI BspMI BsrBI BssHII BssSI
BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI DraI
DraIII DrdI EagI EarI EciI EcoRI FalI FseI
FspI FspAI HpaI KpnI MluI MscI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PsiI PspOMI PspXI PstI PvuII
RsrII SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII Tth111I XbaI XcmI XmnI ZraI