Restriction map for
pF1KE2659
(Linear) (Six-base) MAPSORT of: KIEE2659.seq Check: 6671 from: 1 to: 1470
KIEE2659.orf
With 182 enzymes: SgfI *
January 20, 2017 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1054 1470
Size: 1054 416
AatII G_ACGT'C
Cuts at: 0 77 1470
Size: 77 1393
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 462 1470
Size: 462 1008
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 291 1470
Size: 291 1179
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1118 1150 1470
Size: 1118 32 320
Fragments arranged by size:
1118 320 32
ApaI G_GGCC'C
Cuts at: 0 313 1470
Size: 313 1157
ApoI r'AATT_y
Cuts at: 0 1164 1470
Size: 1164 306
AvaI C'yCGr_G
Cuts at: 0 296 306 383 1470
Size: 296 10 77 1087
Fragments arranged by size:
1087 296 77 10
BamHI G'GATC_C
Cuts at: 0 227 1470
Size: 227 1243
BanI G'GyrC_C
Cuts at: 0 327 1470
Size: 327 1143
BanII G_rGCy'C
Cuts at: 0 209 313 454 1093 1423 1452 1470
Size: 209 104 141 639 330 29 18
Fragments arranged by size:
639 330 209 141 104 29 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 1257 1470
Size: 1257 213
BbvCI CC'TCA_GC
Cuts at: 0 927 1216 1470
Size: 927 289 254
Fragments arranged by size:
927 289 254
BclI T'GATC_A
Cuts at: 0 1242 1352 1470
Size: 1242 110 118
Fragments arranged by size:
1242 118 110
BglI GCCn_nnn'nGGC
Cuts at: 0 204 1470
Size: 204 1266
BglII A'GATC_T
Cuts at: 0 401 1470
Size: 401 1069
Bme1580I G_kGCm'C
Cuts at: 0 313 563 1470
Size: 313 250 907
Fragments arranged by size:
907 313 250
BmgBI CAC'GTC
Cuts at: 0 212 377 564 1470
Size: 212 165 187 906
Fragments arranged by size:
906 212 187 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1107 1470
Size: 1107 363
Bpu10I CC'TnA_GC
Cuts at: 0 927 1216 1417 1422 1470
Size: 927 289 201 5 48
Fragments arranged by size:
927 289 201 48 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 379 1470
Size: 106 273 1091
Fragments arranged by size:
1091 273 106
BsaAI yAC'GTr
Cuts at: 0 509 555 1470
Size: 509 46 915
Fragments arranged by size:
915 509 46
BsaHI Gr'CG_yC
Cuts at: 0 74 1470
Size: 74 1396
BsaWI w'CCGG_w
Cuts at: 0 69 1470
Size: 69 1401
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 345 375 880 910 1470
Size: 345 30 505 30 560
Fragments arranged by size:
560 505 345 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 75 235 301 429 958 1306 1348 1405
Size: 75 160 66 128 529 348 42 57
Cuts at: 1405 1470
Size: 65
Fragments arranged by size:
529 348 160 128 75 66 65 57
42
BseYI C'CCAG_C
Cuts at: 0 19 107 1470
Size: 19 88 1363
Fragments arranged by size:
1363 88 19
BsiEI CG_ry'CG
Cuts at: 0 335 422 1470
Size: 335 87 1048
Fragments arranged by size:
1048 335 87
BsiHKAI G_wGCw'C
Cuts at: 0 382 439 1093 1423 1470
Size: 382 57 654 330 47
Fragments arranged by size:
654 382 330 57 47
BsmI GAATG_Cn'
Cuts at: 0 616 1049 1236 1470
Size: 616 433 187 234
Fragments arranged by size:
616 433 234 187
Bsp1286I G_dGCh'C
Cuts at: 0 209 313 382 439 454 563 1093 1423
Size: 209 104 69 57 15 109 530 330
Cuts at: 1423 1452 1470
Size: 29 18
Fragments arranged by size:
530 330 209 109 104 69 57 29
18 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1054 1470
Size: 1054 416
BsrBI CCG'CTC
Cuts at: 0 225 1470
Size: 225 1245
BsrDI GCAATG_nn'
Cuts at: 0 44 1470
Size: 44 1426
BsrFI r'CCGG_y
Cuts at: 0 198 330 346 1470
Size: 198 132 16 1124
Fragments arranged by size:
1124 198 132 16
BssSI C'ACGA_G
Cuts at: 0 441 1470
Size: 441 1029
BstEII G'GTnAC_C
Cuts at: 0 65 1470
Size: 65 1405
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1337 1470
Size: 1337 133
BstYI r'GATC_y
Cuts at: 0 156 183 227 401 1470
Size: 156 27 44 174 1069
Fragments arranged by size:
1069 174 156 44 27
BtgI C'CryG_G
Cuts at: 0 176 1108 1470
Size: 176 932 362
Fragments arranged by size:
932 362 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 834 894 1269 1470
Size: 834 60 375 201
Fragments arranged by size:
834 375 201 60
BtsI GCAGTG_nn'
Cuts at: 0 984 1241 1470
Size: 984 257 229
Fragments arranged by size:
984 257 229
ClaI AT'CG_AT
Cuts at: 0 934 1470
Size: 934 536
EaeI y'GGCC_r
Cuts at: 0 332 833 1470
Size: 332 501 637
Fragments arranged by size:
637 501 332
EagI C'GGCC_G
Cuts at: 0 332 1470
Size: 332 1138
EarI CTCTTCn'nnn_
Cuts at: 0 1042 1440 1470
Size: 1042 398 30
Fragments arranged by size:
1042 398 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 331 1470
Size: 331 1139
EcoICRI GAG'CTC
Cuts at: 0 1091 1421 1470
Size: 1091 330 49
Fragments arranged by size:
1091 330 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 462 1107 1470
Size: 462 645 363
Fragments arranged by size:
645 462 363
EcoNI CCTnn'n_nnAGG
Cuts at: 0 949 1330 1470
Size: 949 381 140
Fragments arranged by size:
949 381 140
EcoO109I rG'GnC_Cy
Cuts at: 0 1426 1470
Size: 1426 44
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 420 492 1470
Size: 420 72 978
Fragments arranged by size:
978 420 72
FspI TGC'GCA
Cuts at: 0 235 1470
Size: 235 1235
HaeII r_GCGC'y
Cuts at: 0 294 1409 1470
Size: 294 1115 61
Fragments arranged by size:
1115 294 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 345 377 571 603 1405 1437 1470
Size: 345 32 194 32 802 32 33
Fragments arranged by size:
802 345 194 33 32 32 32
HindIII A'AGCT_T
Cuts at: 0 770 1470
Size: 770 700
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 1470
Size: 119 1351
MslI CAynn'nnrTG
Cuts at: 0 820 862 1195 1225 1470
Size: 820 42 333 30 245
Fragments arranged by size:
820 333 245 42 30
MspA1I CmG'CkG
Cuts at: 0 23 194 368 1470
Size: 23 171 174 1102
Fragments arranged by size:
1102 174 171 23
NaeI GCC'GGC
Cuts at: 0 348 1470
Size: 348 1122
NcoI C'CATG_G
Cuts at: 0 1108 1470
Size: 1108 362
NgoMIV G'CCGG_C
Cuts at: 0 346 1470
Size: 346 1124
NspI r_CATG'y
Cuts at: 0 718 1470
Size: 718 752
PasI CC'CwG_GG
Cuts at: 0 32 263 1470
Size: 32 231 1207
Fragments arranged by size:
1207 231 32
PflMI CCAn_nnn'nTGG
Cuts at: 0 524 1470
Size: 524 946
PmlI CAC'GTG
Cuts at: 0 509 1470
Size: 509 961
PpuMI rG'GwC_Cy
Cuts at: 0 1426 1470
Size: 1426 44
PspOMI G'GGCC_C
Cuts at: 0 309 1470
Size: 309 1161
PstI C_TGCA'G
Cuts at: 0 1401 1470
Size: 1401 69
SacI G_AGCT'C
Cuts at: 0 1093 1423 1470
Size: 1093 330 47
Fragments arranged by size:
1093 330 47
SapI GCTCTTCn'nnn_
Cuts at: 0 1440 1470
Size: 1440 30
ScaI AGT'ACT
Cuts at: 0 127 1470
Size: 127 1343
SfcI C'TryA_G
Cuts at: 0 1397 1470
Size: 1397 73
SmaI CCC'GGG
Cuts at: 0 298 308 385 1470
Size: 298 10 77 1085
Fragments arranged by size:
1085 298 77 10
SmlI C'TyrA_G
Cuts at: 0 121 394 1470
Size: 121 273 1076
Fragments arranged by size:
1076 273 121
SspI AAT'ATT
Cuts at: 0 1155 1470
Size: 1155 315
StyI C'CwwG_G
Cuts at: 0 662 1108 1470
Size: 662 446 362
Fragments arranged by size:
662 446 362
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 45 1009 1470
Size: 45 964 461
Fragments arranged by size:
964 461 45
TatI w'GTAC_w
Cuts at: 0 125 548 707 1470
Size: 125 423 159 763
Fragments arranged by size:
763 423 159 125
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 880 912 1035 1067 1470
Size: 880 32 123 32 403
Fragments arranged by size:
880 403 123 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 198 1470
Size: 198 1272
XmaI C'CCGG_G
Cuts at: 0 296 306 383 1470
Size: 296 10 77 1087
Fragments arranged by size:
1087 296 77 10
XmnI GAAnn'nnTTC
Cuts at: 0 403 1470
Size: 403 1067
ZraI GAC'GTC
Cuts at: 0 75 1470
Size: 75 1395
Enzymes that do cut:
AarI AatII AcuI AlfI AloI ApaI ApoI AvaI
BamHI BanI BanII BbsI BbvCI BclI BglI BglII
Bme1580I BmgBI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssSI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HindIII
MmeI MslI MspA1I NaeI NcoI NgoMIV NspI PasI
PflMI PmlI PpuMI PspOMI PstI SacI SapI ScaI
SfcI SmaI SmlI SspI StyI TaqII TatI TstI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BcgI BciVI BfrBI BlpI BmrI BmtI BplI
BsaI BsaBI BsgI BsiWI BsmBI BspEI BspHI BsrGI
BssHII BstAPI BstBI BstZ17I Bsu36I CspCI DraI DraIII
DrdI EcoRI EcoRV FseI FspAI HincII HpaI KasI
KpnI MfeI MluI MscI NarI NdeI NheI NotI
NruI NsiI PacI PciI PfoI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI StuI SwaI Tth111I XbaI XhoI