Restriction map for
pF1KE2626
(Linear) (Six-base) MAPSORT of: KIEE2626.seq Check: 132 from: 1 to: 906
KIEE2626.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 694 906
Size: 694 212
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 280 452 458 906
Size: 26 254 172 6 448
Fragments arranged by size:
448 254 172 26 6
AflII C'TTAA_G
Cuts at: 0 346 906
Size: 346 560
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 185 906
Size: 185 721
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 32 64 167 199 906
Size: 32 32 103 32 707
Fragments arranged by size:
707 103 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 140 906
Size: 140 766
ApaLI G'TGCA_C
Cuts at: 0 707 906
Size: 707 199
ApoI r'AATT_y
Cuts at: 0 55 310 482 579 906
Size: 55 255 172 97 327
Fragments arranged by size:
327 255 172 97 55
BglII A'GATC_T
Cuts at: 0 873 906
Size: 873 33
Bme1580I G_kGCm'C
Cuts at: 0 711 906
Size: 711 195
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 476 906
Size: 476 430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 906
Size: 473 433
BsaBI GATnn'nnATC
Cuts at: 0 70 906
Size: 70 836
BseYI C'CCAG_C
Cuts at: 0 880 906
Size: 880 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 764 906
Size: 153 611 142
Fragments arranged by size:
611 153 142
BsiHKAI G_wGCw'C
Cuts at: 0 711 906
Size: 711 195
Bsp1286I G_dGCh'C
Cuts at: 0 711 906
Size: 711 195
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 694 906
Size: 694 212
BsrDI GCAATG_nn'
Cuts at: 0 605 906
Size: 605 301
BstAPI GCAn_nnn'nTGC
Cuts at: 0 772 906
Size: 772 134
BstYI r'GATC_y
Cuts at: 0 273 397 873 906
Size: 273 124 476 33
Fragments arranged by size:
476 273 124 33
DraIII CAC_nnn'GTG
Cuts at: 0 787 906
Size: 787 119
EarI CTCTTCn'nnn_
Cuts at: 0 152 256 638 906
Size: 152 104 382 268
Fragments arranged by size:
382 268 152 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 280 452 458 906
Size: 26 254 172 6 448
Fragments arranged by size:
448 254 172 26 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 701 906
Size: 701 205
EcoO109I rG'GnC_Cy
Cuts at: 0 733 906
Size: 733 173
EcoRI G'AATT_C
Cuts at: 0 579 906
Size: 579 327
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 188 220 906
Size: 188 32 686
Fragments arranged by size:
686 188 32
HincII GTy'rAC
Cuts at: 0 621 906
Size: 621 285
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 342 906
Size: 342 564
MslI CAynn'nnrTG
Cuts at: 0 167 906
Size: 167 739
MspA1I CmG'CkG
Cuts at: 0 778 866 906
Size: 778 88 40
Fragments arranged by size:
778 88 40
PpuMI rG'GwC_Cy
Cuts at: 0 733 906
Size: 733 173
PstI C_TGCA'G
Cuts at: 0 507 906
Size: 507 399
PvuII CAG'CTG
Cuts at: 0 778 866 906
Size: 778 88 40
Fragments arranged by size:
778 88 40
SapI GCTCTTCn'nnn_
Cuts at: 0 152 906
Size: 152 754
ScaI AGT'ACT
Cuts at: 0 254 906
Size: 254 652
SfcI C'TryA_G
Cuts at: 0 335 379 503 906
Size: 335 44 124 403
Fragments arranged by size:
403 335 124 44
SmlI C'TyrA_G
Cuts at: 0 346 488 906
Size: 346 142 418
Fragments arranged by size:
418 346 142
SspI AAT'ATT
Cuts at: 0 114 906
Size: 114 792
TatI w'GTAC_w
Cuts at: 0 252 906
Size: 252 654
Tth111I GACn'n_nGTC
Cuts at: 0 616 906
Size: 616 290
Enzymes that do cut:
AarI AcuI AflII AlfI AloI AlwNI ApaLI ApoI
BglII Bme1580I BplI BpuEI BsaBI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrDI BstAPI BstYI DraIII EarI Eco57MI
EcoNI EcoO109I EcoRI Hin4I HincII MmeI MslI MspA1I
PpuMI PstI PvuII SapI ScaI SfcI SmlI SspI
TatI Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI BmgBI BmrI BmtI BpmI
Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EciI EcoICRI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
StyI SwaI TaqII TstI XbaI XcmI XhoI XmaI
XmnI ZraI