Restriction map for
pF1KE2618
(Linear) (Six-base) MAPSORT of: KIEE2618.seq Check: 2913 from: 1 to: 1179
KIEE2618.orf
With 182 enzymes: SgfI *
April 12, 2016 09:51 ..
AatII G_ACGT'C
Cuts at: 0 1136 1179
Size: 1136 43
AccI GT'mk_AC
Cuts at: 0 407 1179
Size: 407 772
Acc65I G'GTAC_C
Cuts at: 0 579 1179
Size: 579 600
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 1042 1068 1179
Size: 331 711 26 111
Fragments arranged by size:
711 331 111 26
AflIII A'CryG_T
Cuts at: 0 251 1179
Size: 251 928
AleI CACnn'nnGTG
Cuts at: 0 433 1179
Size: 433 746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 357 1179
Size: 357 822
ApoI r'AATT_y
Cuts at: 0 472 610 1179
Size: 472 138 569
Fragments arranged by size:
569 472 138
BanI G'GyrC_C
Cuts at: 0 150 446 579 1179
Size: 150 296 133 600
Fragments arranged by size:
600 296 150 133
BanII G_rGCy'C
Cuts at: 0 65 1106 1179
Size: 65 1041 73
Fragments arranged by size:
1041 73 65
BbeI G_GCGC'C
Cuts at: 0 154 1179
Size: 154 1025
BbsI GAAGACnn'nnnn_
Cuts at: 0 570 1128 1179
Size: 570 558 51
Fragments arranged by size:
570 558 51
BbvCI CC'TCA_GC
Cuts at: 0 1001 1179
Size: 1001 178
BciVI GTATCCnnnnn_n'
Cuts at: 0 880 1179
Size: 880 299
BglI GCCn_nnn'nGGC
Cuts at: 0 283 1179
Size: 283 896
BlpI GC'TnA_GC
Cuts at: 0 419 463 1179
Size: 419 44 716
Fragments arranged by size:
716 419 44
Bme1580I G_kGCm'C
Cuts at: 0 451 1179
Size: 451 728
BmgBI CAC'GTC
Cuts at: 0 252 426 1179
Size: 252 174 753
Fragments arranged by size:
753 252 174
BmrI ACTGGGnnnn_n'
Cuts at: 0 531 1179
Size: 531 648
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1133 1179
Size: 1133 46
Bpu10I CC'TnA_GC
Cuts at: 0 1001 1179
Size: 1001 178
BsaHI Gr'CG_yC
Cuts at: 0 151 1133 1179
Size: 151 982 46
Fragments arranged by size:
982 151 46
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 357 387 1179
Size: 357 30 792
Fragments arranged by size:
792 357 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 90 365 1179
Size: 90 275 814
Fragments arranged by size:
814 275 90
BseYI C'CCAG_C
Cuts at: 0 172 1179
Size: 172 1007
BsiHKAI G_wGCw'C
Cuts at: 0 274 425 1179
Size: 274 151 754
Fragments arranged by size:
754 274 151
Bsp1286I G_dGCh'C
Cuts at: 0 65 274 425 451 1106 1179
Size: 65 209 151 26 655 73
Fragments arranged by size:
655 209 151 73 65 26
BsrGI T'GTAC_A
Cuts at: 0 626 1179
Size: 626 553
BstYI r'GATC_y
Cuts at: 0 549 746 898 1179
Size: 549 197 152 281
Fragments arranged by size:
549 281 197 152
Bsu36I CC'TnA_GG
Cuts at: 0 377 1179
Size: 377 802
EaeI y'GGCC_r
Cuts at: 0 284 1179
Size: 284 895
EarI CTCTTCn'nnn_
Cuts at: 0 759 1179
Size: 759 420
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 793 1179
Size: 793 386
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 1042 1068 1179
Size: 331 711 26 111
Fragments arranged by size:
711 331 111 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1083 1179
Size: 1083 96
EcoRI G'AATT_C
Cuts at: 0 472 610 1179
Size: 472 138 569
Fragments arranged by size:
569 472 138
HaeII r_GCGC'y
Cuts at: 0 154 846 1179
Size: 154 692 333
Fragments arranged by size:
692 333 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 178 210 357 374 389 406 480 512
Size: 178 32 147 17 15 17 74 32
Cuts at: 512 1179
Size: 667
Fragments arranged by size:
667 178 147 74 32 32 17 17
15
KasI G'GCGC_C
Cuts at: 0 150 1179
Size: 150 1029
KpnI G_GTAC'C
Cuts at: 0 583 1179
Size: 583 596
MfeI C'AATT_G
Cuts at: 0 1010 1179
Size: 1010 169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 636 1179
Size: 242 394 543
Fragments arranged by size:
543 394 242
MslI CAynn'nnrTG
Cuts at: 0 433 1179
Size: 433 746
NarI GG'CG_CC
Cuts at: 0 151 1179
Size: 151 1028
NspI r_CATG'y
Cuts at: 0 344 1179
Size: 344 835
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 85 117 1179
Size: 85 32 1062
Fragments arranged by size:
1062 85 32
SfoI GGC'GCC
Cuts at: 0 152 1179
Size: 152 1027
TatI w'GTAC_w
Cuts at: 0 47 626 817 1179
Size: 47 579 191 362
Fragments arranged by size:
579 362 191 47
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 194 226 854 886 1179
Size: 194 32 628 32 293
Fragments arranged by size:
628 293 194 32 32
ZraI GAC'GTC
Cuts at: 0 1134 1179
Size: 1134 45
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AleI AlfI ApoI
BanI BanII BbeI BbsI BbvCI BciVI BglI BlpI
Bme1580I BmgBI BmrI BplI Bpu10I BsaHI BsaXI BseRI
BseYI BsiHKAI Bsp1286I BsrGI BstYI Bsu36I EaeI EarI
EciI Eco57MI EcoNI EcoRI HaeII Hin4I KasI KpnI
MfeI MmeI MslI NarI NspI PpiI SfoI TatI
TstI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AhdI AloI AlwNI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BcgI BclI BfrBI BglII BmtI BpmI BpuEI
BsaI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoO109I EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI MluI MscI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII Tth111I XbaI XcmI XhoI
XmaI XmnI