Restriction map for
pF1KE2607
(Linear) (Six-base) MAPSORT of: KIEE2607.seq Check: 5897 from: 1 to: 3312
KIEE2607.orf
With 182 enzymes: SgfI *
September 11, 2017 11:55 ..
AccI GT'mk_AC
Cuts at: 0 2150 3312
Size: 2150 1162
Acc65I G'GTAC_C
Cuts at: 0 2761 3312
Size: 2761 551
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 477 490 1230 1309 1485 1693 1813 3312
Size: 477 13 740 79 176 208 120 1499
Fragments arranged by size:
1499 740 477 208 176 120 79 13
AfeI AGC'GCT
Cuts at: 0 2683 3312
Size: 2683 629
AflIII A'CryG_T
Cuts at: 0 1655 2888 3312
Size: 1655 1233 424
Fragments arranged by size:
1655 1233 424
AleI CACnn'nnGTG
Cuts at: 0 1510 3312
Size: 1510 1802
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2685 2717 3312
Size: 2685 32 595
Fragments arranged by size:
2685 595 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1757 1929 3312
Size: 1757 172 1383
Fragments arranged by size:
1757 1383 172
ApoI r'AATT_y
Cuts at: 0 126 141 289 968 1148 1539 2025 3312
Size: 126 15 148 679 180 391 486 1287
Fragments arranged by size:
1287 679 486 391 180 148 126 15
AvaI C'yCGr_G
Cuts at: 0 993 1868 2060 2299 2854 3312
Size: 993 875 192 239 555 458
Fragments arranged by size:
993 875 555 458 239 192
BamHI G'GATC_C
Cuts at: 0 2660 3312
Size: 2660 652
BanI G'GyrC_C
Cuts at: 0 238 410 451 547 1673 2286 2742 2761
Size: 238 172 41 96 1126 613 456 19
Cuts at: 2761 2950 3312
Size: 189 362
Fragments arranged by size:
1126 613 456 362 238 189 172 96
41 19
BanII G_rGCy'C
Cuts at: 0 2720 3312
Size: 2720 592
BbeI G_GCGC'C
Cuts at: 0 455 3312
Size: 455 2857
BbsI GAAGACnn'nnnn_
Cuts at: 0 156 1619 1801 1916 2202 2220 3042 3110
Size: 156 1463 182 115 286 18 822 68
Cuts at: 3110 3149 3274 3312
Size: 39 125 38
Fragments arranged by size:
1463 822 286 182 156 125 115 68
39 38 18
BbvCI CC'TCA_GC
Cuts at: 0 695 3312
Size: 695 2617
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1807 1841 3312
Size: 1807 34 1471
Fragments arranged by size:
1807 1471 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3249 3312
Size: 3249 63
BglII A'GATC_T
Cuts at: 0 488 3312
Size: 488 2824
BlpI GC'TnA_GC
Cuts at: 0 22 3312
Size: 22 3290
Bme1580I G_kGCm'C
Cuts at: 0 2747 2953 3312
Size: 2747 206 359
Fragments arranged by size:
2747 359 206
BmgBI CAC'GTC
Cuts at: 0 1656 2754 3312
Size: 1656 1098 558
Fragments arranged by size:
1656 1098 558
BmrI ACTGGGnnnn_n'
Cuts at: 0 340 708 2741 2804 3312
Size: 340 368 2033 63 508
Fragments arranged by size:
2033 508 368 340 63
BmtI G_CTAG'C
Cuts at: 0 1022 3312
Size: 1022 2290
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 1321 1453 1990 2706 3312
Size: 60 1261 132 537 716 606
Fragments arranged by size:
1261 716 606 537 132 60
Bpu10I CC'TnA_GC
Cuts at: 0 695 3312
Size: 695 2617
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3259 3312
Size: 3259 53
BsaI GGTCTCn'nnnn_
Cuts at: 0 663 1858 3312
Size: 663 1195 1454
Fragments arranged by size:
1454 1195 663
BsaAI yAC'GTr
Cuts at: 0 2262 2361 2768 3312
Size: 2262 99 407 544
Fragments arranged by size:
2262 544 407 99
BsaBI GATnn'nnATC
Cuts at: 0 2590 2866 3312
Size: 2590 276 446
Fragments arranged by size:
2590 446 276
BsaHI Gr'CG_yC
Cuts at: 0 302 452 3312
Size: 302 150 2860
Fragments arranged by size:
2860 302 150
BsaWI w'CCGG_w
Cuts at: 0 830 1517 1604 3312
Size: 830 687 87 1708
Fragments arranged by size:
1708 830 687 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 184 214 3312
Size: 184 30 3098
Fragments arranged by size:
3098 184 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 631 1126 1129 1549 1858 3312
Size: 631 495 3 420 309 1454
Fragments arranged by size:
1454 631 495 420 309 3
BseYI C'CCAG_C
Cuts at: 0 401 1195 1831 2009 2409 3312
Size: 401 794 636 178 400 903
Fragments arranged by size:
903 794 636 401 400 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1743 2769 3025 3312
Size: 1743 1026 256 287
Fragments arranged by size:
1743 1026 287 256
BsiHKAI G_wGCw'C
Cuts at: 0 94 2837 3312
Size: 94 2743 475
Fragments arranged by size:
2743 475 94
BsmBI CGTCTCn'nnnn_
Cuts at: 0 89 3312
Size: 89 3223
Bsp1286I G_dGCh'C
Cuts at: 0 94 2720 2747 2837 2953 3312
Size: 94 2626 27 90 116 359
Fragments arranged by size:
2626 359 116 94 90 27
BspEI T'CCGG_A
Cuts at: 0 830 1604 3312
Size: 830 774 1708
Fragments arranged by size:
1708 830 774
BspHI T'CATG_A
Cuts at: 0 3173 3312
Size: 3173 139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2849 2927 3312
Size: 2849 78 385
Fragments arranged by size:
2849 385 78
BsrDI GCAATG_nn'
Cuts at: 0 1221 2047 3312
Size: 1221 826 1265
Fragments arranged by size:
1265 1221 826
BsrFI r'CCGG_y
Cuts at: 0 497 3312
Size: 497 2815
BssSI C'ACGA_G
Cuts at: 0 784 3312
Size: 784 2528
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1680 2586 2841 3312
Size: 1680 906 255 471
Fragments arranged by size:
1680 906 471 255
BstXI CCAn_nnnn'nTGG
Cuts at: 0 922 2754 2874 3312
Size: 922 1832 120 438
Fragments arranged by size:
1832 922 438 120
BstYI r'GATC_y
Cuts at: 0 488 741 893 2660 3312
Size: 488 253 152 1767 652
Fragments arranged by size:
1767 652 488 253 152
Bsu36I CC'TnA_GG
Cuts at: 0 1109 2665 3312
Size: 1109 1556 647
Fragments arranged by size:
1556 1109 647
BtgI C'CryG_G
Cuts at: 0 188 3312
Size: 188 3124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 495 704 1919 1989 2220 3312
Size: 495 209 1215 70 231 1092
Fragments arranged by size:
1215 1092 495 231 209 70
BtsI GCAGTG_nn'
Cuts at: 0 2781 3312
Size: 2781 531
ClaI AT'CG_AT
Cuts at: 0 2594 3240 3312
Size: 2594 646 72
Fragments arranged by size:
2594 646 72
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 867 902 3312
Size: 867 35 2410
Fragments arranged by size:
2410 867 35
DraIII CAC_nnn'GTG
Cuts at: 0 1656 1892 2818 3312
Size: 1656 236 926 494
Fragments arranged by size:
1656 926 494 236
EaeI y'GGCC_r
Cuts at: 0 629 1623 1802 2648 2726 2736 3312
Size: 629 994 179 846 78 10 576
Fragments arranged by size:
994 846 629 576 179 78 10
EarI CTCTTCn'nnn_
Cuts at: 0 1492 1590 1696 2338 2460 3312
Size: 1492 98 106 642 122 852
Fragments arranged by size:
1492 852 642 122 106 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1411 3312
Size: 1411 1901
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 477 490 1230 1309 1321 1453 1485
Size: 60 417 13 740 79 12 132 32
Cuts at: 1485 1693 1813 1990 2706 3312
Size: 208 120 177 716 606
Fragments arranged by size:
740 716 606 417 208 177 132 120
79 60 32 13 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1027 3004 3312
Size: 1027 1977 308
Fragments arranged by size:
1977 1027 308
EcoO109I rG'GnC_Cy
Cuts at: 0 2560 3312
Size: 2560 752
EcoRI G'AATT_C
Cuts at: 0 1539 3312
Size: 1539 1773
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2680 3099 3312
Size: 2680 419 213
Fragments arranged by size:
2680 419 213
FspI TGC'GCA
Cuts at: 0 484 3312
Size: 484 2828
HaeII r_GCGC'y
Cuts at: 0 455 2685 3312
Size: 455 2230 627
Fragments arranged by size:
2230 627 455
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 146 178 3312
Size: 146 32 3134
Fragments arranged by size:
3134 146 32
HincII GTy'rAC
Cuts at: 0 2151 2916 3312
Size: 2151 765 396
Fragments arranged by size:
2151 765 396
HindIII A'AGCT_T
Cuts at: 0 1291 1786 1946 3262 3312
Size: 1291 495 160 1316 50
Fragments arranged by size:
1316 1291 495 160 50
KasI G'GCGC_C
Cuts at: 0 451 3312
Size: 451 2861
KpnI G_GTAC'C
Cuts at: 0 2765 3312
Size: 2765 547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 902 1108 1517 3312
Size: 686 216 206 409 1795
Fragments arranged by size:
1795 686 409 216 206
MscI TGG'CCA
Cuts at: 0 631 1804 2650 2728 3312
Size: 631 1173 846 78 584
Fragments arranged by size:
1173 846 631 584 78
MslI CAynn'nnrTG
Cuts at: 0 439 1510 2887 3070 3312
Size: 439 1071 1377 183 242
Fragments arranged by size:
1377 1071 439 242 183
MspA1I CmG'CkG
Cuts at: 0 1287 1314 1926 3312
Size: 1287 27 612 1386
Fragments arranged by size:
1386 1287 612 27
NaeI GCC'GGC
Cuts at: 0 499 3312
Size: 499 2813
NarI GG'CG_CC
Cuts at: 0 452 3312
Size: 452 2860
NgoMIV G'CCGG_C
Cuts at: 0 497 3312
Size: 497 2815
NheI G'CTAG_C
Cuts at: 0 1018 3312
Size: 1018 2294
NspI r_CATG'y
Cuts at: 0 483 2892 3312
Size: 483 2409 420
Fragments arranged by size:
2409 483 420
PasI CC'CwG_GG
Cuts at: 0 1773 3312
Size: 1773 1539
PciI A'CATG_T
Cuts at: 0 2888 3312
Size: 2888 424
PflMI CCAn_nnn'nTGG
Cuts at: 0 231 2075 3312
Size: 231 1844 1237
Fragments arranged by size:
1844 1237 231
PfoI T'CCnGG_A
Cuts at: 0 755 2215 2957 3312
Size: 755 1460 742 355
Fragments arranged by size:
1460 755 742 355
PpuMI rG'GwC_Cy
Cuts at: 0 2560 3312
Size: 2560 752
PsiI TTA'TAA
Cuts at: 0 2572 3312
Size: 2572 740
PstI C_TGCA'G
Cuts at: 0 1529 2582 3019 3312
Size: 1529 1053 437 293
Fragments arranged by size:
1529 1053 437 293
PvuII CAG'CTG
Cuts at: 0 1287 1314 1926 3312
Size: 1287 27 612 1386
Fragments arranged by size:
1386 1287 612 27
SalI G'TCGA_C
Cuts at: 0 2149 3312
Size: 2149 1163
ScaI AGT'ACT
Cuts at: 0 3013 3312
Size: 3013 299
SexAI A'CCwGG_T
Cuts at: 0 428 2972 3312
Size: 428 2544 340
Fragments arranged by size:
2544 428 340
SfcI C'TryA_G
Cuts at: 0 1525 2578 3015 3312
Size: 1525 1053 437 297
Fragments arranged by size:
1525 1053 437 297
SfoI GGC'GCC
Cuts at: 0 453 3312
Size: 453 2859
SmaI CCC'GGG
Cuts at: 0 1870 3312
Size: 1870 1442
SmlI C'TyrA_G
Cuts at: 0 3274 3312
Size: 3274 38
SnaBI TAC'GTA
Cuts at: 0 2361 3312
Size: 2361 951
SphI G_CATG'C
Cuts at: 0 483 3312
Size: 483 2829
SspI AAT'ATT
Cuts at: 0 624 3312
Size: 624 2688
StyI C'CwwG_G
Cuts at: 0 182 284 915 2108 3312
Size: 182 102 631 1193 1204
Fragments arranged by size:
1204 1193 631 182 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 256 438 565 1480 2694 3312
Size: 256 182 127 915 1214 618
Fragments arranged by size:
1214 915 618 256 182 127
TatI w'GTAC_w
Cuts at: 0 71 569 2312 3011 3312
Size: 71 498 1743 699 301
Fragments arranged by size:
1743 699 498 301 71
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 184 216 726 737 758 769 3312
Size: 184 32 510 11 21 11 2543
Fragments arranged by size:
2543 510 184 32 21 11 11
Tth111I GACn'n_nGTC
Cuts at: 0 2974 3312
Size: 2974 338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2907 3312
Size: 2907 405
XmaI C'CCGG_G
Cuts at: 0 1868 3312
Size: 1868 1444
XmnI GAAnn'nnTTC
Cuts at: 0 3082 3312
Size: 3082 230
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AleI AloI AlwNI
ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrDI BsrFI BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII EaeI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NheI NspI PasI
PciI PflMI PfoI PpuMI PsiI PstI PvuII SalI
ScaI SexAI SfcI SfoI SmaI SmlI SnaBI SphI
SspI StyI TaqII TatI TstI Tth111I XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AhdI AlfI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BclI BfrBI
BglI BplI BsiEI BsiWI BsmI BsrBI BsrGI BssHII
BstBI BstEII BstZ17I DraI DrdI EagI EcoICRI EcoRV
FseI FspAI HpaI MfeI MluI NcoI NdeI NotI
NruI NsiI PacI PmeI PmlI PpiI PshAI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SanDI SapI
SbfI SfiI SgfI SgrAI SpeI SrfI StuI SwaI
XbaI XhoI ZraI