Restriction map for
pF1KE2600
(Linear) (Six-base) MAPSORT of: KIEE2600.seq Check: 10 from: 1 to: 891
KIEE2600.orf
With 182 enzymes: SgfI *
January 12, 2016 10:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 891
Size: 709 182
AfeI AGC'GCT
Cuts at: 0 259 571 891
Size: 259 312 320
Fragments arranged by size:
320 312 259
AhdI GACnn_n'nnGTC
Cuts at: 0 588 891
Size: 588 303
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 604 636 891
Size: 604 32 255
Fragments arranged by size:
604 255 32
AlwNI CAG_nnn'CTG
Cuts at: 0 318 891
Size: 318 573
ApaLI G'TGCA_C
Cuts at: 0 137 865 891
Size: 137 728 26
Fragments arranged by size:
728 137 26
AvaI C'yCGr_G
Cuts at: 0 90 891
Size: 90 801
BanII G_rGCy'C
Cuts at: 0 91 338 472 891
Size: 91 247 134 419
Fragments arranged by size:
419 247 134 91
BbsI GAAGACnn'nnnn_
Cuts at: 0 449 677 891
Size: 449 228 214
Fragments arranged by size:
449 228 214
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 118 152 891
Size: 118 34 739
Fragments arranged by size:
739 118 34
Bme1580I G_kGCm'C
Cuts at: 0 141 869 891
Size: 141 728 22
Fragments arranged by size:
728 141 22
BmgBI CAC'GTC
Cuts at: 0 215 864 891
Size: 215 649 27
Fragments arranged by size:
649 215 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 141 891
Size: 141 750
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 465 891
Size: 383 82 426
Fragments arranged by size:
426 383 82
BsaBI GATnn'nnATC
Cuts at: 0 535 891
Size: 535 356
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 457 487 604 634 891
Size: 457 30 117 30 257
Fragments arranged by size:
457 257 117 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 609 891
Size: 609 282
BsiEI CG_ry'CG
Cuts at: 0 307 330 891
Size: 307 23 561
Fragments arranged by size:
561 307 23
BsiHKAI G_wGCw'C
Cuts at: 0 91 141 601 869 891
Size: 91 50 460 268 22
Fragments arranged by size:
460 268 91 50 22
BsmBI CGTCTCn'nnnn_
Cuts at: 0 483 891
Size: 483 408
Bsp1286I G_dGCh'C
Cuts at: 0 91 141 338 472 601 869 891
Size: 91 50 197 134 129 268 22
Fragments arranged by size:
268 197 134 129 91 50 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 386 891
Size: 386 505
BsrFI r'CCGG_y
Cuts at: 0 66 454 891
Size: 66 388 437
Fragments arranged by size:
437 388 66
BsrGI T'GTAC_A
Cuts at: 0 720 891
Size: 720 171
BssHII G'CGCG_C
Cuts at: 0 152 369 891
Size: 152 217 522
Fragments arranged by size:
522 217 152
BstAPI GCAn_nnn'nTGC
Cuts at: 0 318 891
Size: 318 573
BtgI C'CryG_G
Cuts at: 0 57 107 330 891
Size: 57 50 223 561
Fragments arranged by size:
561 223 57 50
BtsI GCAGTG_nn'
Cuts at: 0 465 772 867 891
Size: 465 307 95 24
Fragments arranged by size:
465 307 95 24
DraIII CAC_nnn'GTG
Cuts at: 0 495 891
Size: 495 396
EaeI y'GGCC_r
Cuts at: 0 327 891
Size: 327 564
EagI C'GGCC_G
Cuts at: 0 327 891
Size: 327 564
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 149 891
Size: 149 742
EcoICRI GAG'CTC
Cuts at: 0 89 891
Size: 89 802
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 465 709 891
Size: 383 82 244 182
Fragments arranged by size:
383 244 182 82
EcoNI CCTnn'n_nnAGG
Cuts at: 0 779 833 891
Size: 779 54 58
Fragments arranged by size:
779 58 54
EcoO109I rG'GnC_Cy
Cuts at: 0 103 891
Size: 103 788
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 813 891
Size: 813 78
FspI TGC'GCA
Cuts at: 0 219 891
Size: 219 672
FspAI rTGC'GCAy
Cuts at: 0 219 891
Size: 219 672
HaeII r_GCGC'y
Cuts at: 0 261 319 391 455 573 891
Size: 261 58 72 64 118 318
Fragments arranged by size:
318 261 118 72 64 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 5 37 457 489 574 606 891
Size: 5 32 420 32 85 32 285
Fragments arranged by size:
420 285 85 32 32 32 5
HincII GTy'rAC
Cuts at: 0 345 582 891
Size: 345 237 309
Fragments arranged by size:
345 309 237
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 891
Size: 416 475
MspA1I CmG'CkG
Cuts at: 0 6 31 59 309 332 891
Size: 6 25 28 250 23 559
Fragments arranged by size:
559 250 28 25 23 6
NaeI GCC'GGC
Cuts at: 0 456 891
Size: 456 435
NgoMIV G'CCGG_C
Cuts at: 0 454 891
Size: 454 437
NspI r_CATG'y
Cuts at: 0 556 882 891
Size: 556 326 9
Fragments arranged by size:
556 326 9
PasI CC'CwG_GG
Cuts at: 0 713 891
Size: 713 178
PflMI CCAn_nnn'nTGG
Cuts at: 0 23 891
Size: 23 868
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 604 636 891
Size: 604 32 255
Fragments arranged by size:
604 255 32
PpuMI rG'GwC_Cy
Cuts at: 0 103 891
Size: 103 788
PsiI TTA'TAA
Cuts at: 0 755 891
Size: 755 136
PstI C_TGCA'G
Cuts at: 0 314 891
Size: 314 577
SacI G_AGCT'C
Cuts at: 0 91 891
Size: 91 800
SacII CC_GC'GG
Cuts at: 0 60 333 891
Size: 60 273 558
Fragments arranged by size:
558 273 60
SanDI GG'GwC_CC
Cuts at: 0 103 891
Size: 103 788
SfcI C'TryA_G
Cuts at: 0 310 873 891
Size: 310 563 18
Fragments arranged by size:
563 310 18
SgrAI Cr'CCGG_yG
Cuts at: 0 66 891
Size: 66 825
SphI G_CATG'C
Cuts at: 0 556 882 891
Size: 556 326 9
Fragments arranged by size:
556 326 9
StyI C'CwwG_G
Cuts at: 0 848 891
Size: 848 43
TatI w'GTAC_w
Cuts at: 0 720 891
Size: 720 171
Enzymes that do cut:
AcuI AfeI AhdI AloI AlwNI ApaLI AvaI BanII
BbsI BcgI Bme1580I BmgBI BmrI BpmI BsaBI BsaXI
BseRI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BsrGI
BssHII BstAPI BtgI BtsI DraIII EaeI EagI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII
Hin4I HincII MmeI MspA1I NaeI NgoMIV NspI PasI
PflMI PpiI PpuMI PsiI PstI SacI SacII SanDI
SfcI SgrAI SphI StyI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AleI AlfI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BciVI BclI BfrBI
BglI BglII BlpI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BseYI BsgI BsiWI BsmI BspEI
BspHI BsrBI BsrDI BssSI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EarI
EcoRI EcoRV FseI HindIII HpaI KasI KpnI MfeI
MluI MscI MslI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PfoI PmeI PmlI PshAI
PspOMI PspXI PsrI PvuI PvuII RsrII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SmaI SmlI
SnaBI SpeI SrfI SspI StuI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI