Restriction map for
pF1KE2587
(Linear) (Six-base) MAPSORT of: KIEE2587.seq Check: 3175 from: 1 to: 1968
KIEE2587.orf
With 182 enzymes: SgfI *
August 13, 2015 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1491 1968
Size: 1491 477
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 950 1270 1968
Size: 950 320 698
Fragments arranged by size:
950 698 320
AhdI GACnn_n'nnGTC
Cuts at: 0 153 1968
Size: 153 1815
AleI CACnn'nnGTG
Cuts at: 0 397 514 565 1968
Size: 397 117 51 1403
Fragments arranged by size:
1403 397 117 51
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 338 370 1968
Size: 338 32 1598
Fragments arranged by size:
1598 338 32
AlwNI CAG_nnn'CTG
Cuts at: 0 151 529 1968
Size: 151 378 1439
Fragments arranged by size:
1439 378 151
ApaLI G'TGCA_C
Cuts at: 0 220 1968
Size: 220 1748
ApoI r'AATT_y
Cuts at: 0 878 997 1027 1459 1552 1935 1968
Size: 878 119 30 432 93 383 33
Fragments arranged by size:
878 432 383 119 93 33 30
BamHI G'GATC_C
Cuts at: 0 431 1968
Size: 431 1537
BanI G'GyrC_C
Cuts at: 0 111 304 1968
Size: 111 193 1664
Fragments arranged by size:
1664 193 111
BanII G_rGCy'C
Cuts at: 0 897 1968
Size: 897 1071
BbsI GAAGACnn'nnnn_
Cuts at: 0 71 1138 1477 1968
Size: 71 1067 339 491
Fragments arranged by size:
1067 491 339 71
BbvCI CC'TCA_GC
Cuts at: 0 1045 1968
Size: 1045 923
BclI T'GATC_A
Cuts at: 0 533 923 1968
Size: 533 390 1045
Fragments arranged by size:
1045 533 390
BfrBI ATG'CAT
Cuts at: 0 48 649 1968
Size: 48 601 1319
Fragments arranged by size:
1319 601 48
BglI GCCn_nnn'nGGC
Cuts at: 0 970 1968
Size: 970 998
BlpI GC'TnA_GC
Cuts at: 0 706 1968
Size: 706 1262
Bme1580I G_kGCm'C
Cuts at: 0 114 224 1968
Size: 114 110 1744
Fragments arranged by size:
1744 114 110
BmrI ACTGGGnnnn_n'
Cuts at: 0 51 220 508 560 1968
Size: 51 169 288 52 1408
Fragments arranged by size:
1408 288 169 52 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1775 1968
Size: 1775 193
Bpu10I CC'TnA_GC
Cuts at: 0 1045 1968
Size: 1045 923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 965 1968
Size: 965 1003
BsaI GGTCTCn'nnnn_
Cuts at: 0 251 1968
Size: 251 1717
BsaBI GATnn'nnATC
Cuts at: 0 907 1968
Size: 907 1061
BsaWI w'CCGG_w
Cuts at: 0 619 1968
Size: 619 1349
BseYI C'CCAG_C
Cuts at: 0 402 1968
Size: 402 1566
BsiHKAI G_wGCw'C
Cuts at: 0 224 897 1968
Size: 224 673 1071
Fragments arranged by size:
1071 673 224
BsmI GAATG_Cn'
Cuts at: 0 1262 1548 1968
Size: 1262 286 420
Fragments arranged by size:
1262 420 286
Bsp1286I G_dGCh'C
Cuts at: 0 114 224 897 1968
Size: 114 110 673 1071
Fragments arranged by size:
1071 673 114 110
BspEI T'CCGG_A
Cuts at: 0 619 1968
Size: 619 1349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1491 1968
Size: 1491 477
BsrDI GCAATG_nn'
Cuts at: 0 548 688 1430 1639 1677 1853 1968
Size: 548 140 742 209 38 176 115
Fragments arranged by size:
742 548 209 176 140 115 38
BsrGI T'GTAC_A
Cuts at: 0 1115 1825 1968
Size: 1115 710 143
Fragments arranged by size:
1115 710 143
BstAPI GCAn_nnn'nTGC
Cuts at: 0 811 1968
Size: 811 1157
BstEII G'GTnAC_C
Cuts at: 0 183 1968
Size: 183 1785
BstXI CCAn_nnnn'nTGG
Cuts at: 0 342 516 567 1968
Size: 342 174 51 1401
Fragments arranged by size:
1401 342 174 51
BstYI r'GATC_y
Cuts at: 0 431 985 1968
Size: 431 554 983
Fragments arranged by size:
983 554 431
BtgI C'CryG_G
Cuts at: 0 341 1038 1094 1968
Size: 341 697 56 874
Fragments arranged by size:
874 697 341 56
BtsI GCAGTG_nn'
Cuts at: 0 510 1734 1968
Size: 510 1224 234
Fragments arranged by size:
1224 510 234
ClaI AT'CG_AT
Cuts at: 0 1307 1968
Size: 1307 661
DraI TTT'AAA
Cuts at: 0 753 1280 1464 1968
Size: 753 527 184 504
Fragments arranged by size:
753 527 504 184
DraIII CAC_nnn'GTG
Cuts at: 0 228 1968
Size: 228 1740
EaeI y'GGCC_r
Cuts at: 0 479 1968
Size: 479 1489
EcoICRI GAG'CTC
Cuts at: 0 895 1968
Size: 895 1073
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 950 1270 1775 1968
Size: 950 320 505 193
Fragments arranged by size:
950 505 320 193
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1498 1968
Size: 1498 470
EcoO109I rG'GnC_Cy
Cuts at: 0 1192 1968
Size: 1192 776
EcoRI G'AATT_C
Cuts at: 0 878 1968
Size: 878 1090
EcoRV GAT'ATC
Cuts at: 0 1617 1968
Size: 1617 351
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 709 790 1968
Size: 709 81 1178
Fragments arranged by size:
1178 709 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 139 171 342 374 1968
Size: 139 32 171 32 1594
Fragments arranged by size:
1594 171 139 32 32
HindIII A'AGCT_T
Cuts at: 0 932 1679 1968
Size: 932 747 289
Fragments arranged by size:
932 747 289
MfeI C'AATT_G
Cuts at: 0 1010 1968
Size: 1010 958
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 1968
Size: 437 1531
MscI TGG'CCA
Cuts at: 0 481 1968
Size: 481 1487
MslI CAynn'nnrTG
Cuts at: 0 397 487 514 565 829 1968
Size: 397 90 27 51 264 1139
Fragments arranged by size:
1139 397 264 90 51 27
NcoI C'CATG_G
Cuts at: 0 1038 1094 1968
Size: 1038 56 874
Fragments arranged by size:
1038 874 56
NdeI CA'TA_TG
Cuts at: 0 651 843 1332 1968
Size: 651 192 489 636
Fragments arranged by size:
651 636 489 192
NsiI A_TGCA'T
Cuts at: 0 50 651 1968
Size: 50 601 1317
Fragments arranged by size:
1317 601 50
NspI r_CATG'y
Cuts at: 0 33 369 1968
Size: 33 336 1599
Fragments arranged by size:
1599 336 33
PflMI CCAn_nnn'nTGG
Cuts at: 0 195 232 1968
Size: 195 37 1736
Fragments arranged by size:
1736 195 37
PfoI T'CCnGG_A
Cuts at: 0 1718 1968
Size: 1718 250
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 898 930 1968
Size: 898 32 1038
Fragments arranged by size:
1038 898 32
PpuMI rG'GwC_Cy
Cuts at: 0 1192 1968
Size: 1192 776
PstI C_TGCA'G
Cuts at: 0 447 1499 1968
Size: 447 1052 469
Fragments arranged by size:
1052 469 447
SacI G_AGCT'C
Cuts at: 0 897 1968
Size: 897 1071
SfcI C'TryA_G
Cuts at: 0 443 779 1397 1495 1968
Size: 443 336 618 98 473
Fragments arranged by size:
618 473 443 336 98
SmlI C'TyrA_G
Cuts at: 0 980 1968
Size: 980 988
SphI G_CATG'C
Cuts at: 0 33 1968
Size: 33 1935
SspI AAT'ATT
Cuts at: 0 1006 1288 1968
Size: 1006 282 680
Fragments arranged by size:
1006 680 282
StyI C'CwwG_G
Cuts at: 0 189 1038 1094 1968
Size: 189 849 56 874
Fragments arranged by size:
874 849 189 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 239 577 1968
Size: 239 338 1391
Fragments arranged by size:
1391 338 239
TatI w'GTAC_w
Cuts at: 0 1115 1327 1825 1968
Size: 1115 212 498 143
Fragments arranged by size:
1115 498 212 143
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 279 1968
Size: 279 1689
Enzymes that do cut:
AarI AcuI AhdI AleI AloI AlwNI ApaLI ApoI
BamHI BanI BanII BbsI BbvCI BclI BfrBI BglI
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaBI
BsaWI BseYI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI
BsrGI BstAPI BstEII BstXI BstYI BtgI BtsI ClaI
DraI DraIII EaeI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI Hin4I HindIII MfeI MmeI MscI MslI
NcoI NdeI NsiI NspI PflMI PfoI PpiI PpuMI
PstI SacI SfcI SmlI SphI SspI StyI TaqII
TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AlfI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BcgI BciVI BglII BmgBI BmtI BplI BsaAI
BsaHI BsaXI BseRI BsgI BsiEI BsiWI BsmBI BspHI
BsrBI BsrFI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI
CspCI DrdI EagI EarI EciI FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MluI MspA1I NaeI
NarI NgoMIV NheI NotI NruI PacI PasI PciI
PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI StuI SwaI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI