Restriction map for
pF1KE2582
(Linear) (Six-base) MAPSORT of: KIEE2582.seq Check: 5008 from: 1 to: 2868
KIEE2582.orf
With 182 enzymes: SgfI *
June 20, 2019 10:05 ..
AccI GT'mk_AC
Cuts at: 0 455 1465 2868
Size: 455 1010 1403
Fragments arranged by size:
1403 1010 455
Acc65I G'GTAC_C
Cuts at: 0 1088 2868
Size: 1088 1780
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 585 769 778 1360 1411 2868
Size: 585 184 9 582 51 1457
Fragments arranged by size:
1457 585 582 184 51 9
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2506 2540 2836 2868
Size: 2506 34 296 32
Fragments arranged by size:
2506 296 34 32
AlwNI CAG_nnn'CTG
Cuts at: 0 303 1845 2119 2128 2401 2868
Size: 303 1542 274 9 273 467
Fragments arranged by size:
1542 467 303 274 273 9
ApaI G_GGCC'C
Cuts at: 0 881 2868
Size: 881 1987
ApoI r'AATT_y
Cuts at: 0 1433 1654 2868
Size: 1433 221 1214
Fragments arranged by size:
1433 1214 221
AvaI C'yCGr_G
Cuts at: 0 843 917 2830 2868
Size: 843 74 1913 38
Fragments arranged by size:
1913 843 74 38
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 919 952 2868
Size: 919 33 1916
Fragments arranged by size:
1916 919 33
BamHI G'GATC_C
Cuts at: 0 2027 2868
Size: 2027 841
BanI G'GyrC_C
Cuts at: 0 328 667 1088 2868
Size: 328 339 421 1780
Fragments arranged by size:
1780 421 339 328
BanII G_rGCy'C
Cuts at: 0 881 1323 2185 2654 2868
Size: 881 442 862 469 214
Fragments arranged by size:
881 862 469 442 214
BbsI GAAGACnn'nnnn_
Cuts at: 0 104 446 1533 1619 2350 2473 2868
Size: 104 342 1087 86 731 123 395
Fragments arranged by size:
1087 731 395 342 123 104 86
BbvCI CC'TCA_GC
Cuts at: 0 542 1055 2447 2553 2868
Size: 542 513 1392 106 315
Fragments arranged by size:
1392 542 513 315 106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2308 2342 2868
Size: 2308 34 526
Fragments arranged by size:
2308 526 34
BglI GCCn_nnn'nGGC
Cuts at: 0 2041 2868
Size: 2041 827
BlpI GC'TnA_GC
Cuts at: 0 781 2695 2868
Size: 781 1914 173
Fragments arranged by size:
1914 781 173
Bme1580I G_kGCm'C
Cuts at: 0 670 881 2598 2868
Size: 670 211 1717 270
Fragments arranged by size:
1717 670 270 211
BmgBI CAC'GTC
Cuts at: 0 771 2868
Size: 771 2097
BmrI ACTGGGnnnn_n'
Cuts at: 0 68 961 2411 2868
Size: 68 893 1450 457
Fragments arranged by size:
1450 893 457 68
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2538 2868
Size: 2538 330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 829 1930 2059 2095 2209 2340 2782 2868
Size: 829 1101 129 36 114 131 442 86
Fragments arranged by size:
1101 829 442 131 129 114 86 36
Bpu10I CC'TnA_GC
Cuts at: 0 542 1055 2447 2553 2868
Size: 542 513 1392 106 315
Fragments arranged by size:
1392 542 513 315 106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 2868
Size: 419 2449
BsaI GGTCTCn'nnnn_
Cuts at: 0 1541 2868
Size: 1541 1327
BsaWI w'CCGG_w
Cuts at: 0 527 2868
Size: 527 2341
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 208 238 1198 1228 2053 2083 2868
Size: 208 30 960 30 825 30 785
Fragments arranged by size:
960 825 785 208 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 589 1043 1363 1804 2035 2092 2224 2227
Size: 589 454 320 441 231 57 132 3
Cuts at: 2227 2230 2343 2868
Size: 3 113 525
Fragments arranged by size:
589 525 454 441 320 231 132 113
57 3 3
BseYI C'CCAG_C
Cuts at: 0 890 1276 1322 1850 1937 1989 2496 2662
Size: 890 386 46 528 87 52 507 166
Cuts at: 2662 2680 2733 2868
Size: 18 53 135
Fragments arranged by size:
890 528 507 386 166 135 87 53
52 46 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 1189 2245 2806 2868
Size: 404 785 1056 561 62
Fragments arranged by size:
1056 785 561 404 62
BsiEI CG_ry'CG
Cuts at: 0 2691 2868
Size: 2691 177
BsiHKAI G_wGCw'C
Cuts at: 0 989 1251 2086 2185 2510 2654 2701 2868
Size: 989 262 835 99 325 144 47 167
Fragments arranged by size:
989 835 325 262 167 144 99 47
BsmI GAATG_Cn'
Cuts at: 0 2112 2868
Size: 2112 756
Bsp1286I G_dGCh'C
Cuts at: 0 670 881 989 1251 1323 2086 2185 2510
Size: 670 211 108 262 72 763 99 325
Cuts at: 2510 2598 2654 2701 2868
Size: 88 56 47 167
Fragments arranged by size:
763 670 325 262 211 167 108 99
88 72 56 47
BspHI T'CATG_A
Cuts at: 0 1533 2868
Size: 1533 1335
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 889 2868
Size: 889 1979
BsrBI CCG'CTC
Cuts at: 0 776 1890 2025 2375 2868
Size: 776 1114 135 350 493
Fragments arranged by size:
1114 776 493 350 135
BsrFI r'CCGG_y
Cuts at: 0 2690 2868
Size: 2690 178
BssSI C'ACGA_G
Cuts at: 0 1027 2868
Size: 1027 1841
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2119 2868
Size: 2119 749
BstEII G'GTnAC_C
Cuts at: 0 1003 2868
Size: 1003 1865
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1015 1288 2868
Size: 1015 273 1580
Fragments arranged by size:
1580 1015 273
BstYI r'GATC_y
Cuts at: 0 204 242 1736 2027 2868
Size: 204 38 1494 291 841
Fragments arranged by size:
1494 841 291 204 38
Bsu36I CC'TnA_GG
Cuts at: 0 238 2868
Size: 238 2630
BtgI C'CryG_G
Cuts at: 0 419 645 2685 2778 2868
Size: 419 226 2040 93 90
Fragments arranged by size:
2040 419 226 93 90
BtsI GCAGTG_nn'
Cuts at: 0 1201 1703 2117 2530 2598 2868
Size: 1201 502 414 413 68 270
Fragments arranged by size:
1201 502 414 413 270 68
DraIII CAC_nnn'GTG
Cuts at: 0 498 2868
Size: 498 2370
EaeI y'GGCC_r
Cuts at: 0 356 1076 1956 2688 2738 2775 2868
Size: 356 720 880 732 50 37 93
Fragments arranged by size:
880 732 720 356 93 50 37
EagI C'GGCC_G
Cuts at: 0 2688 2868
Size: 2688 180
EarI CTCTTCn'nnn_
Cuts at: 0 1191 1781 2456 2659 2868
Size: 1191 590 675 203 209
Fragments arranged by size:
1191 675 590 209 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1179 2868
Size: 1179 1689
EcoICRI GAG'CTC
Cuts at: 0 2183 2652 2868
Size: 2183 469 216
Fragments arranged by size:
2183 469 216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 585 769 778 829 1360 1411 1930 2059
Size: 585 184 9 51 531 51 519 129
Cuts at: 2059 2095 2209 2340 2782 2868
Size: 36 114 131 442 86
Fragments arranged by size:
585 531 519 442 184 131 129 114
86 51 51 36 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1744 2868
Size: 1744 1124
EcoO109I rG'GnC_Cy
Cuts at: 0 50 877 878 922 1842 2310 2868
Size: 50 827 1 44 920 468 558
Fragments arranged by size:
920 827 558 468 50 44 1
EcoRI G'AATT_C
Cuts at: 0 1654 2868
Size: 1654 1214
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 226 2868
Size: 226 2642
HaeII r_GCGC'y
Cuts at: 0 748 2868
Size: 748 2120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2397 2429 2694 2726 2792 2824 2868
Size: 2397 32 265 32 66 32 44
Fragments arranged by size:
2397 265 66 44 32 32 32
KpnI G_GTAC'C
Cuts at: 0 1092 2868
Size: 1092 1776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2115 2868
Size: 2115 753
MscI TGG'CCA
Cuts at: 0 358 1958 2777 2868
Size: 358 1600 819 91
Fragments arranged by size:
1600 819 358 91
MslI CAynn'nnrTG
Cuts at: 0 1952 2516 2868
Size: 1952 564 352
Fragments arranged by size:
1952 564 352
MspA1I CmG'CkG
Cuts at: 0 283 300 801 1196 1854 2128 2442 2687
Size: 283 17 501 395 658 274 314 245
Cuts at: 2687 2737 2868
Size: 50 131
Fragments arranged by size:
658 501 395 314 283 274 245 131
50 17
NaeI GCC'GGC
Cuts at: 0 2692 2868
Size: 2692 176
NcoI C'CATG_G
Cuts at: 0 645 2778 2868
Size: 645 2133 90
Fragments arranged by size:
2133 645 90
NdeI CA'TA_TG
Cuts at: 0 1812 2868
Size: 1812 1056
NgoMIV G'CCGG_C
Cuts at: 0 2690 2868
Size: 2690 178
NspI r_CATG'y
Cuts at: 0 1868 1974 2521 2868
Size: 1868 106 547 347
Fragments arranged by size:
1868 547 347 106
PasI CC'CwG_GG
Cuts at: 0 1015 1242 2868
Size: 1015 227 1626
Fragments arranged by size:
1626 1015 227
PflMI CCAn_nnn'nTGG
Cuts at: 0 1032 1329 2868
Size: 1032 297 1539
Fragments arranged by size:
1539 1032 297
PfoI T'CCnGG_A
Cuts at: 0 308 1739 1997 2868
Size: 308 1431 258 871
Fragments arranged by size:
1431 871 308 258
PpuMI rG'GwC_Cy
Cuts at: 0 50 922 2310 2868
Size: 50 872 1388 558
Fragments arranged by size:
1388 872 558 50
PspOMI G'GGCC_C
Cuts at: 0 877 2868
Size: 877 1991
PstI C_TGCA'G
Cuts at: 0 900 1626 1838 1859 2124 2318 2868
Size: 900 726 212 21 265 194 550
Fragments arranged by size:
900 726 550 265 212 194 21
PvuII CAG'CTG
Cuts at: 0 283 801 1854 2128 2442 2868
Size: 283 518 1053 274 314 426
Fragments arranged by size:
1053 518 426 314 283 274
SacI G_AGCT'C
Cuts at: 0 2185 2654 2868
Size: 2185 469 214
Fragments arranged by size:
2185 469 214
SacII CC_GC'GG
Cuts at: 0 2688 2868
Size: 2688 180
SanDI GG'GwC_CC
Cuts at: 0 50 2868
Size: 50 2818
SapI GCTCTTCn'nnn_
Cuts at: 0 2659 2868
Size: 2659 209
SbfI CC_TGCA'GG
Cuts at: 0 900 2868
Size: 900 1968
SexAI A'CCwGG_T
Cuts at: 0 494 2868
Size: 494 2374
SfcI C'TryA_G
Cuts at: 0 896 1622 1834 1855 2120 2314 2868
Size: 896 726 212 21 265 194 554
Fragments arranged by size:
896 726 554 265 212 194 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2041 2868
Size: 2041 827
SmlI C'TyrA_G
Cuts at: 0 434 2868
Size: 434 2434
SphI G_CATG'C
Cuts at: 0 2521 2868
Size: 2521 347
StyI C'CwwG_G
Cuts at: 0 645 2778 2868
Size: 645 2133 90
Fragments arranged by size:
2133 645 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1234 2562 2747 2868
Size: 1234 1328 185 121
Fragments arranged by size:
1328 1234 185 121
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1198 1230 1695 1727 2868
Size: 1198 32 465 32 1141
Fragments arranged by size:
1198 1141 465 32 32
XbaI T'CTAG_A
Cuts at: 0 93 2868
Size: 93 2775
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1945 2161 2439 2868
Size: 1945 216 278 429
Fragments arranged by size:
1945 429 278 216
Enzymes that do cut:
AccI Acc65I AcuI AlfI AlwNI ApaI ApoI AvaI
BaeI BamHI BanI BanII BbsI BbvCI BcgI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I KpnI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NspI PasI PflMI PfoI
PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI
SbfI SexAI SfcI SfiI SmlI SphI StyI TaqII
TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaLI AscI AseI AsiSI AvrII BbeI
BciVI BclI BfrBI BglII BmtI BsaAI BsaBI BsaHI
BsiWI BsmBI BspEI BsrDI BsrGI BssHII BstBI BstZ17I
BtgZI ClaI CspCI DraI DrdI EcoRV FseI FspI
FspAI HincII HindIII HpaI KasI MfeI MluI NarI
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI
ScaI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI TatI Tth111I XhoI XmaI XmnI
ZraI