Restriction map for
pF1KE2575
(Linear) (Six-base) MAPSORT of: KIEE2575.seq Check: 9665 from: 1 to: 525
KIEE2575.orf
With 182 enzymes: SgfI *
May 26, 2015 10:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 525
Size: 105 420
AhdI GACnn_n'nnGTC
Cuts at: 0 480 525
Size: 480 45
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 421 453 525
Size: 421 32 72
Fragments arranged by size:
421 72 32
ApaI G_GGCC'C
Cuts at: 0 250 525
Size: 250 275
AvaI C'yCGr_G
Cuts at: 0 23 307 525
Size: 23 284 218
Fragments arranged by size:
284 218 23
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 91 124 525
Size: 91 33 401
Fragments arranged by size:
401 91 33
BanII G_rGCy'C
Cuts at: 0 250 525
Size: 250 275
BbsI GAAGACnn'nnnn_
Cuts at: 0 258 525
Size: 258 267
BbvCI CC'TCA_GC
Cuts at: 0 422 525
Size: 422 103
Bme1580I G_kGCm'C
Cuts at: 0 250 525
Size: 250 275
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 198 525
Size: 198 327
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 525
Size: 401 124
Bpu10I CC'TnA_GC
Cuts at: 0 422 525
Size: 422 103
BsaI GGTCTCn'nnnn_
Cuts at: 0 169 525
Size: 169 356
BsaHI Gr'CG_yC
Cuts at: 0 224 525
Size: 224 301
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 421 451 525
Size: 421 30 74
Fragments arranged by size:
421 74 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 226 229 525
Size: 226 3 296
Fragments arranged by size:
296 226 3
BseYI C'CCAG_C
Cuts at: 0 185 189 346 525
Size: 185 4 157 179
Fragments arranged by size:
185 179 157 4
BsmBI CGTCTCn'nnnn_
Cuts at: 0 503 525
Size: 503 22
Bsp1286I G_dGCh'C
Cuts at: 0 250 525
Size: 250 275
BstAPI GCAn_nnn'nTGC
Cuts at: 0 155 525
Size: 155 370
BstXI CCAn_nnnn'nTGG
Cuts at: 0 459 525
Size: 459 66
Bsu36I CC'TnA_GG
Cuts at: 0 169 525
Size: 169 356
BtgI C'CryG_G
Cuts at: 0 452 504 525
Size: 452 52 21
Fragments arranged by size:
452 52 21
EaeI y'GGCC_r
Cuts at: 0 2 525
Size: 2 523
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 401 525
Size: 105 296 124
Fragments arranged by size:
296 124 105
EcoNI CCTnn'n_nnAGG
Cuts at: 0 397 525
Size: 397 128
EcoO109I rG'GnC_Cy
Cuts at: 0 20 246 247 525
Size: 20 226 1 278
Fragments arranged by size:
278 226 20 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 397 429 466 498 525
Size: 397 32 37 32 27
Fragments arranged by size:
397 37 32 32 27
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 525
Size: 92 433
MscI TGG'CCA
Cuts at: 0 4 525
Size: 4 521
MspA1I CmG'CkG
Cuts at: 0 125 189 350 421 525
Size: 125 64 161 71 104
Fragments arranged by size:
161 125 104 71 64
NcoI C'CATG_G
Cuts at: 0 452 504 525
Size: 452 52 21
Fragments arranged by size:
452 52 21
NspI r_CATG'y
Cuts at: 0 339 525
Size: 339 186
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 421 453 525
Size: 421 32 72
Fragments arranged by size:
421 72 32
PpuMI rG'GwC_Cy
Cuts at: 0 20 525
Size: 20 505
PspOMI G'GGCC_C
Cuts at: 0 246 525
Size: 246 279
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 460 492 525
Size: 460 32 33
Fragments arranged by size:
460 33 32
PvuII CAG'CTG
Cuts at: 0 125 189 421 525
Size: 125 64 232 104
Fragments arranged by size:
232 125 104 64
SanDI GG'GwC_CC
Cuts at: 0 20 525
Size: 20 505
SmaI CCC'GGG
Cuts at: 0 25 525
Size: 25 500
StyI C'CwwG_G
Cuts at: 0 452 504 525
Size: 452 52 21
Fragments arranged by size:
452 52 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 465 517 525
Size: 465 52 8
Fragments arranged by size:
465 52 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 186 218 525
Size: 186 32 307
Fragments arranged by size:
307 186 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 142 525
Size: 142 383
XmaI C'CCGG_G
Cuts at: 0 23 525
Size: 23 502
Enzymes that do cut:
AcuI AhdI AloI ApaI AvaI BaeI BanII BbsI
BbvCI Bme1580I BplI BpmI Bpu10I BsaI BsaHI BsaXI
BseRI BseYI BsmBI Bsp1286I BstAPI BstXI Bsu36I BtgI
EaeI Eco57MI EcoNI EcoO109I Hin4I MmeI MscI MspA1I
NcoI NspI PpiI PpuMI PspOMI PsrI PvuII SanDI
SmaI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BamHI BanI BbeI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BpuEI
BsaAI BsaBI BsaWI BsgI BsiEI BsiHKAI BsiWI BsmI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstYI BstZ17I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MslI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PshAI PsiI
PspXI PstI PvuI RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XhoI XmnI ZraI