Restriction map for
pF1KE2551
(Linear) (Six-base) MAPSORT of: KIEE2551.seq Check: 3729 from: 1 to: 1890
KIEE2551.orf
With 182 enzymes: SgfI *
December 2, 2014 13:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1423 1890
Size: 1423 467
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 786 1575 1890
Size: 704 82 789 315
Fragments arranged by size:
789 704 315 82
AflIII A'CryG_T
Cuts at: 0 1365 1890
Size: 1365 525
AgeI A'CCGG_T
Cuts at: 0 719 1890
Size: 719 1171
AleI CACnn'nnGTG
Cuts at: 0 208 1345 1890
Size: 208 1137 545
Fragments arranged by size:
1137 545 208
AlwNI CAG_nnn'CTG
Cuts at: 0 741 809 1531 1890
Size: 741 68 722 359
Fragments arranged by size:
741 722 359 68
ApaI G_GGCC'C
Cuts at: 0 1496 1890
Size: 1496 394
ApoI r'AATT_y
Cuts at: 0 18 688 1856 1890
Size: 18 670 1168 34
Fragments arranged by size:
1168 670 34 18
AvaI C'yCGr_G
Cuts at: 0 1596 1890
Size: 1596 294
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1704 1737 1890
Size: 1704 33 153
Fragments arranged by size:
1704 153 33
BamHI G'GATC_C
Cuts at: 0 1426 1890
Size: 1426 464
BanI G'GyrC_C
Cuts at: 0 895 1890
Size: 895 995
BanII G_rGCy'C
Cuts at: 0 410 734 1496 1678 1890
Size: 410 324 762 182 212
Fragments arranged by size:
762 410 324 212 182
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 419 453 1890
Size: 419 34 1437
Fragments arranged by size:
1437 419 34
BclI T'GATC_A
Cuts at: 0 1337 1790 1890
Size: 1337 453 100
Fragments arranged by size:
1337 453 100
BfrBI ATG'CAT
Cuts at: 0 440 1890
Size: 440 1450
BglII A'GATC_T
Cuts at: 0 995 1890
Size: 995 895
BlpI GC'TnA_GC
Cuts at: 0 215 1890
Size: 215 1675
Bme1580I G_kGCm'C
Cuts at: 0 1496 1890
Size: 1496 394
BmgBI CAC'GTC
Cuts at: 0 674 705 1368 1572 1890
Size: 674 31 663 204 318
Fragments arranged by size:
674 663 318 204 31
BmrI ACTGGGnnnn_n'
Cuts at: 0 424 1890
Size: 424 1466
BmtI G_CTAG'C
Cuts at: 0 1046 1890
Size: 1046 844
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 929 936 982 1495 1629 1737 1784
Size: 718 211 7 46 513 134 108 47
Cuts at: 1784 1890
Size: 106
Fragments arranged by size:
718 513 211 134 108 106 47 46
7
BsaI GGTCTCn'nnnn_
Cuts at: 0 232 1890
Size: 232 1658
BsaAI yAC'GTr
Cuts at: 0 1226 1461 1890
Size: 1226 235 429
Fragments arranged by size:
1226 429 235
BsaBI GATnn'nnATC
Cuts at: 0 1291 1890
Size: 1291 599
BsaHI Gr'CG_yC
Cuts at: 0 10 818 1890
Size: 10 808 1072
Fragments arranged by size:
1072 808 10
BsaWI w'CCGG_w
Cuts at: 0 719 1890
Size: 719 1171
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 547 577 1890
Size: 547 30 1313
Fragments arranged by size:
1313 547 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 555 1204 1492 1890
Size: 343 212 649 288 398
Fragments arranged by size:
649 398 343 288 212
BseYI C'CCAG_C
Cuts at: 0 189 1207 1299 1619 1890
Size: 189 1018 92 320 271
Fragments arranged by size:
1018 320 271 189 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 609 1890
Size: 609 1281
BsiHKAI G_wGCw'C
Cuts at: 0 410 1148 1400 1505 1890
Size: 410 738 252 105 385
Fragments arranged by size:
738 410 385 252 105
BsmI GAATG_Cn'
Cuts at: 0 346 1887 1890
Size: 346 1541 3
Fragments arranged by size:
1541 346 3
Bsp1286I G_dGCh'C
Cuts at: 0 410 734 1148 1400 1496 1505 1678 1890
Size: 410 324 414 252 96 9 173 212
Fragments arranged by size:
414 410 324 252 212 173 96 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1192 1423 1890
Size: 1192 231 467
Fragments arranged by size:
1192 467 231
BsrBI CCG'CTC
Cuts at: 0 393 991 1389 1890
Size: 393 598 398 501
Fragments arranged by size:
598 501 398 393
BsrDI GCAATG_nn'
Cuts at: 0 306 500 512 1890
Size: 306 194 12 1378
Fragments arranged by size:
1378 306 194 12
BsrFI r'CCGG_y
Cuts at: 0 719 1890
Size: 719 1171
BstEII G'GTnAC_C
Cuts at: 0 1086 1434 1890
Size: 1086 348 456
Fragments arranged by size:
1086 456 348
BstXI CCAn_nnnn'nTGG
Cuts at: 0 210 433 1370 1890
Size: 210 223 937 520
Fragments arranged by size:
937 520 223 210
BstYI r'GATC_y
Cuts at: 0 713 995 1426 1613 1890
Size: 713 282 431 187 277
Fragments arranged by size:
713 431 282 277 187
BtgI C'CryG_G
Cuts at: 0 1403 1487 1890
Size: 1403 84 403
Fragments arranged by size:
1403 403 84
BtsI GCAGTG_nn'
Cuts at: 0 1505 1890
Size: 1505 385
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1248 1283 1890
Size: 1248 35 607
Fragments arranged by size:
1248 607 35
DraI TTT'AAA
Cuts at: 0 1812 1890
Size: 1812 78
EaeI y'GGCC_r
Cuts at: 0 371 1190 1625 1890
Size: 371 819 435 265
Fragments arranged by size:
819 435 371 265
EarI CTCTTCn'nnn_
Cuts at: 0 934 1222 1890
Size: 934 288 668
Fragments arranged by size:
934 668 288
EcoICRI GAG'CTC
Cuts at: 0 408 1890
Size: 408 1482
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 718 786 929 936 982 1495 1575
Size: 704 14 68 143 7 46 513 80
Cuts at: 1575 1629 1737 1784 1890
Size: 54 108 47 106
Fragments arranged by size:
704 513 143 108 106 80 68 54
47 46 14 7
EcoO109I rG'GnC_Cy
Cuts at: 0 738 1026 1204 1454 1492 1890
Size: 738 288 178 250 38 398
Fragments arranged by size:
738 398 288 250 178 38
EcoRI G'AATT_C
Cuts at: 0 18 1890
Size: 18 1872
HaeII r_GCGC'y
Cuts at: 0 312 550 1383 1890
Size: 312 238 833 507
Fragments arranged by size:
833 507 312 238
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1118 1150 1418 1450 1782 1814 1890
Size: 1118 32 268 32 332 32 76
Fragments arranged by size:
1118 332 268 76 32 32 32
MfeI C'AATT_G
Cuts at: 0 945 1890
Size: 945 945
MscI TGG'CCA
Cuts at: 0 373 1192 1627 1890
Size: 373 819 435 263
Fragments arranged by size:
819 435 373 263
MslI CAynn'nnrTG
Cuts at: 0 208 367 431 535 1345 1890
Size: 208 159 64 104 810 545
Fragments arranged by size:
810 545 208 159 104 64
MspA1I CmG'CkG
Cuts at: 0 582 596 755 762 1890
Size: 582 14 159 7 1128
Fragments arranged by size:
1128 582 159 14 7
NdeI CA'TA_TG
Cuts at: 0 322 1890
Size: 322 1568
NheI G'CTAG_C
Cuts at: 0 1042 1890
Size: 1042 848
NsiI A_TGCA'T
Cuts at: 0 442 1890
Size: 442 1448
NspI r_CATG'y
Cuts at: 0 1251 1890
Size: 1251 639
PasI CC'CwG_GG
Cuts at: 0 100 1890
Size: 100 1790
PflMI CCAn_nnn'nTGG
Cuts at: 0 157 741 1890
Size: 157 584 1149
Fragments arranged by size:
1149 584 157
PfoI T'CCnGG_A
Cuts at: 0 607 734 959 1890
Size: 607 127 225 931
Fragments arranged by size:
931 607 225 127
PpuMI rG'GwC_Cy
Cuts at: 0 738 1026 1204 1890
Size: 738 288 178 686
Fragments arranged by size:
738 686 288 178
PshAI GACnn'nnGTC
Cuts at: 0 1202 1890
Size: 1202 688
PspOMI G'GGCC_C
Cuts at: 0 1492 1890
Size: 1492 398
PstI C_TGCA'G
Cuts at: 0 713 1567 1890
Size: 713 854 323
Fragments arranged by size:
854 713 323
PvuII CAG'CTG
Cuts at: 0 582 596 755 762 1890
Size: 582 14 159 7 1128
Fragments arranged by size:
1128 582 159 14 7
SacI G_AGCT'C
Cuts at: 0 410 1890
Size: 410 1480
SfcI C'TryA_G
Cuts at: 0 79 709 1563 1890
Size: 79 630 854 327
Fragments arranged by size:
854 630 327 79
SmaI CCC'GGG
Cuts at: 0 1598 1890
Size: 1598 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 834 1611 1890
Size: 834 777 279
Fragments arranged by size:
834 777 279
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 207 1890
Size: 207 1683
XmaI C'CCGG_G
Cuts at: 0 1596 1890
Size: 1596 294
Enzymes that do cut:
AarI AcuI AflIII AgeI AleI AlwNI ApaI ApoI
AvaI BaeI BamHI BanI BanII BcgI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BstEII
BstXI BstYI BtgI BtsI CspCI DraI EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I MfeI MscI
MslI MspA1I NdeI NheI NsiI NspI PasI PflMI
PfoI PpuMI PshAI PspOMI PstI PvuII SacI SfcI
SmaI TaqII XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AhdI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BbeI BbsI
BbvCI BciVI BglI BplI Bpu10I BpuEI BsiEI BsiWI
BsmBI BspEI BspHI BsrGI BssHII BssSI BstAPI BstBI
BstZ17I Bsu36I BtgZI ClaI DraIII DrdI EagI EciI
EcoNI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MluI MmeI NaeI NarI NcoI
NgoMIV NotI NruI PacI PciI PmeI PmlI PpiI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI