Restriction map for
pF1KE2518
(Linear) (Six-base) MAPSORT of: KIEE2518.seq Check: 6563 from: 1 to: 4368
KIEE2518.orf
With 182 enzymes: SgfI *
February 23, 2015 11:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 401 1177 4368
Size: 401 776 3191
Fragments arranged by size:
3191 776 401
AccI GT'mk_AC
Cuts at: 0 469 4368
Size: 469 3899
AclI AA'CG_TT
Cuts at: 0 1330 4368
Size: 1330 3038
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2150 2420 4368
Size: 2150 270 1948
Fragments arranged by size:
2150 1948 270
AfeI AGC'GCT
Cuts at: 0 100 4368
Size: 100 4268
AflII C'TTAA_G
Cuts at: 0 1668 4368
Size: 1668 2700
AflIII A'CryG_T
Cuts at: 0 2645 3053 3453 4242 4368
Size: 2645 408 400 789 126
Fragments arranged by size:
2645 789 408 400 126
AhdI GACnn_n'nnGTC
Cuts at: 0 3079 4368
Size: 3079 1289
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1631 1663 2175 2207 3041 3073 4368
Size: 1631 32 512 32 834 32 1295
Fragments arranged by size:
1631 1295 834 512 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1005 2398 3020 4368
Size: 1005 1393 622 1348
Fragments arranged by size:
1393 1348 1005 622
ApaLI G'TGCA_C
Cuts at: 0 4246 4368
Size: 4246 122
ApoI r'AATT_y
Cuts at: 0 169 286 634 1041 1216 1767 1864 2490
Size: 169 117 348 407 175 551 97 626
Cuts at: 2490 2712 2847 3337 4368
Size: 222 135 490 1031
Fragments arranged by size:
1031 626 551 490 407 348 222 175
169 135 117 97
AseI AT'TA_AT
Cuts at: 0 2104 2300 4368
Size: 2104 196 2068
Fragments arranged by size:
2104 2068 196
AvaI C'yCGr_G
Cuts at: 0 2027 4368
Size: 2027 2341
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2766 2799 4368
Size: 2766 33 1569
Fragments arranged by size:
2766 1569 33
BamHI G'GATC_C
Cuts at: 0 3402 4368
Size: 3402 966
BanI G'GyrC_C
Cuts at: 0 719 1951 4264 4368
Size: 719 1232 2313 104
Fragments arranged by size:
2313 1232 719 104
BanII G_rGCy'C
Cuts at: 0 761 2046 3551 4368
Size: 761 1285 1505 817
Fragments arranged by size:
1505 1285 817 761
BbeI G_GCGC'C
Cuts at: 0 1955 4268 4368
Size: 1955 2313 100
Fragments arranged by size:
2313 1955 100
BbsI GAAGACnn'nnnn_
Cuts at: 0 1634 3604 3781 4004 4145 4368
Size: 1634 1970 177 223 141 223
Fragments arranged by size:
1970 1634 223 223 177 141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1173 1207 2462 2496 4368
Size: 1173 34 1255 34 1872
Fragments arranged by size:
1872 1255 1173 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1210 4368
Size: 1210 3158
BclI T'GATC_A
Cuts at: 0 2594 4368
Size: 2594 1774
BfrBI ATG'CAT
Cuts at: 0 3418 4368
Size: 3418 950
BglII A'GATC_T
Cuts at: 0 419 4326 4368
Size: 419 3907 42
Fragments arranged by size:
3907 419 42
BlpI GC'TnA_GC
Cuts at: 0 755 4368
Size: 755 3613
Bme1580I G_kGCm'C
Cuts at: 0 4250 4368
Size: 4250 118
BmgBI CAC'GTC
Cuts at: 0 3917 4243 4368
Size: 3917 326 125
Fragments arranged by size:
3917 326 125
BmrI ACTGGGnnnn_n'
Cuts at: 0 116 255 1015 1425 1890 2223 3035 3105
Size: 116 139 760 410 465 333 812 70
Cuts at: 3105 3543 3774 4368
Size: 438 231 594
Fragments arranged by size:
812 760 594 465 438 410 333 231
139 116 70
BmtI G_CTAG'C
Cuts at: 0 2066 4368
Size: 2066 2302
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1175 4368
Size: 1175 3193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 3254 4368
Size: 432 2822 1114
Fragments arranged by size:
2822 1114 432
Bpu10I CC'TnA_GC
Cuts at: 0 763 2360 4368
Size: 763 1597 2008
Fragments arranged by size:
2008 1597 763
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2363 4241 4368
Size: 2363 1878 127
Fragments arranged by size:
2363 1878 127
BsaI GGTCTCn'nnnn_
Cuts at: 0 2958 3948 4368
Size: 2958 990 420
Fragments arranged by size:
2958 990 420
BsaAI yAC'GTr
Cuts at: 0 2646 4368
Size: 2646 1722
BsaHI Gr'CG_yC
Cuts at: 0 1952 4265 4368
Size: 1952 2313 103
Fragments arranged by size:
2313 1952 103
BsaWI w'CCGG_w
Cuts at: 0 1938 3939 4368
Size: 1938 2001 429
Fragments arranged by size:
2001 1938 429
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 29 1905 1935 2175 2205 2358 2388 3252
Size: 29 1876 30 240 30 153 30 864
Cuts at: 3252 3282 4368
Size: 30 1086
Fragments arranged by size:
1876 1086 864 240 153 30 30 30
30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2366 3092 4368
Size: 2366 726 1276
Fragments arranged by size:
2366 1276 726
BseYI C'CCAG_C
Cuts at: 0 553 1235 4368
Size: 553 682 3133
Fragments arranged by size:
3133 682 553
BsiEI CG_ry'CG
Cuts at: 0 562 4368
Size: 562 3806
BsiHKAI G_wGCw'C
Cuts at: 0 448 761 2046 4250 4368
Size: 448 313 1285 2204 118
Fragments arranged by size:
2204 1285 448 313 118
BsmI GAATG_Cn'
Cuts at: 0 505 1611 3395 4368
Size: 505 1106 1784 973
Fragments arranged by size:
1784 1106 973 505
Bsp1286I G_dGCh'C
Cuts at: 0 448 761 2046 3551 4250 4368
Size: 448 313 1285 1505 699 118
Fragments arranged by size:
1505 1285 699 448 313 118
BspEI T'CCGG_A
Cuts at: 0 1938 4368
Size: 1938 2430
BspHI T'CATG_A
Cuts at: 0 780 3326 4098 4368
Size: 780 2546 772 270
Fragments arranged by size:
2546 780 772 270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 401 1177 3217 4368
Size: 401 776 2040 1151
Fragments arranged by size:
2040 1151 776 401
BsrDI GCAATG_nn'
Cuts at: 0 489 3606 3618 4368
Size: 489 3117 12 750
Fragments arranged by size:
3117 750 489 12
BsrFI r'CCGG_y
Cuts at: 0 1108 4368
Size: 1108 3260
BssSI C'ACGA_G
Cuts at: 0 546 4330 4368
Size: 546 3784 38
Fragments arranged by size:
3784 546 38
BstAPI GCAn_nnn'nTGC
Cuts at: 0 502 2572 4368
Size: 502 2070 1796
Fragments arranged by size:
2070 1796 502
BstBI TT'CG_AA
Cuts at: 0 3792 4368
Size: 3792 576
BstEII G'GTnAC_C
Cuts at: 0 908 2272 3106 4368
Size: 908 1364 834 1262
Fragments arranged by size:
1364 1262 908 834
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3399 4368
Size: 3399 969
BstYI r'GATC_y
Cuts at: 0 419 3402 3579 3969 4326 4368
Size: 419 2983 177 390 357 42
Fragments arranged by size:
2983 419 390 357 177 42
BstZ17I GTA'TAC
Cuts at: 0 470 4368
Size: 470 3898
Bsu36I CC'TnA_GG
Cuts at: 0 822 4368
Size: 822 3546
BtgI C'CryG_G
Cuts at: 0 2459 3723 4368
Size: 2459 1264 645
Fragments arranged by size:
2459 1264 645
BtsI GCAGTG_nn'
Cuts at: 0 119 651 877 1191 4368
Size: 119 532 226 314 3177
Fragments arranged by size:
3177 532 314 226 119
DraI TTT'AAA
Cuts at: 0 213 1040 4368
Size: 213 827 3328
Fragments arranged by size:
3328 827 213
DrdI GACnn_nn'nnGTC
Cuts at: 0 845 4368
Size: 845 3523
EaeI y'GGCC_r
Cuts at: 0 1099 4163 4368
Size: 1099 3064 205
Fragments arranged by size:
3064 1099 205
EarI CTCTTCn'nnn_
Cuts at: 0 1739 4368
Size: 1739 2629
EcoICRI GAG'CTC
Cuts at: 0 759 2044 4368
Size: 759 1285 2324
Fragments arranged by size:
2324 1285 759
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 2150 2420 3254 4368
Size: 432 1718 270 834 1114
Fragments arranged by size:
1718 1114 834 432 270
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2168 4368
Size: 2168 2200
EcoO109I rG'GnC_Cy
Cuts at: 0 1319 4368
Size: 1319 3049
EcoRI G'AATT_C
Cuts at: 0 1216 1767 4368
Size: 1216 551 2601
Fragments arranged by size:
2601 1216 551
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3001 4368
Size: 3001 1367
HaeII r_GCGC'y
Cuts at: 0 102 1955 4268 4368
Size: 102 1853 2313 100
Fragments arranged by size:
2313 1853 102 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 145 177 2554 2586 2632 2664 3065 3097
Size: 145 32 2377 32 46 32 401 32
Cuts at: 3097 3252 3284 3474 3506 4160 4192 4368
Size: 155 32 190 32 654 32 176
Fragments arranged by size:
2377 654 401 190 176 155 145 46
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 3954 4368
Size: 3954 414
HindIII A'AGCT_T
Cuts at: 0 651 1968 2924 3370 3773 4368
Size: 651 1317 956 446 403 595
Fragments arranged by size:
1317 956 651 595 446 403
KasI G'GCGC_C
Cuts at: 0 1951 4264 4368
Size: 1951 2313 104
Fragments arranged by size:
2313 1951 104
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 2824 3272 4181 4368
Size: 542 2282 448 909 187
Fragments arranged by size:
2282 909 542 448 187
MslI CAynn'nnrTG
Cuts at: 0 1133 2800 2829 3423 3611 4180 4368
Size: 1133 1667 29 594 188 569 188
Fragments arranged by size:
1667 1133 594 569 188 188 29
MspA1I CmG'CkG
Cuts at: 0 672 863 928 4368
Size: 672 191 65 3440
Fragments arranged by size:
3440 672 191 65
NarI GG'CG_CC
Cuts at: 0 1952 4265 4368
Size: 1952 2313 103
Fragments arranged by size:
2313 1952 103
NcoI C'CATG_G
Cuts at: 0 2459 3723 4368
Size: 2459 1264 645
Fragments arranged by size:
2459 1264 645
NdeI CA'TA_TG
Cuts at: 0 2154 2205 3232 4368
Size: 2154 51 1027 1136
Fragments arranged by size:
2154 1136 1027 51
NheI G'CTAG_C
Cuts at: 0 2062 4368
Size: 2062 2306
NsiI A_TGCA'T
Cuts at: 0 3420 4368
Size: 3420 948
NspI r_CATG'y
Cuts at: 0 3569 4009 4368
Size: 3569 440 359
Fragments arranged by size:
3569 440 359
PflMI CCAn_nnn'nTGG
Cuts at: 0 3884 4368
Size: 3884 484
PfoI T'CCnGG_A
Cuts at: 0 960 4368
Size: 960 3408
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1738 1770 2175 2207 4368
Size: 1738 32 405 32 2161
Fragments arranged by size:
2161 1738 405 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1319 4368
Size: 1319 3049
PshAI GACnn'nnGTC
Cuts at: 0 884 4368
Size: 884 3484
PsiI TTA'TAA
Cuts at: 0 4232 4368
Size: 4232 136
PstI C_TGCA'G
Cuts at: 0 1174 1513 3434 3831 4368
Size: 1174 339 1921 397 537
Fragments arranged by size:
1921 1174 537 397 339
PvuII CAG'CTG
Cuts at: 0 928 4368
Size: 928 3440
SacI G_AGCT'C
Cuts at: 0 761 2046 4368
Size: 761 1285 2322
Fragments arranged by size:
2322 1285 761
SanDI GG'GwC_CC
Cuts at: 0 1319 4368
Size: 1319 3049
SbfI CC_TGCA'GG
Cuts at: 0 1174 4368
Size: 1174 3194
SfcI C'TryA_G
Cuts at: 0 1170 1509 2853 3408 3430 3827 4368
Size: 1170 339 1344 555 22 397 541
Fragments arranged by size:
1344 1170 555 541 397 339 22
SfoI GGC'GCC
Cuts at: 0 1953 4266 4368
Size: 1953 2313 102
Fragments arranged by size:
2313 1953 102
SmlI C'TyrA_G
Cuts at: 0 1668 2027 2378 4256 4368
Size: 1668 359 351 1878 112
Fragments arranged by size:
1878 1668 359 351 112
SpeI A'CTAG_T
Cuts at: 0 1654 4312 4368
Size: 1654 2658 56
Fragments arranged by size:
2658 1654 56
SspI AAT'ATT
Cuts at: 0 378 1684 4032 4341 4368
Size: 378 1306 2348 309 27
Fragments arranged by size:
2348 1306 378 309 27
StuI AGG'CCT
Cuts at: 0 1674 4368
Size: 1674 2694
StyI C'CwwG_G
Cuts at: 0 1476 2459 3723 4368
Size: 1476 983 1264 645
Fragments arranged by size:
1476 1264 983 645
SwaI ATTT'AAAT
Cuts at: 0 213 4368
Size: 213 4155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 168 1774 1916 2038 2396 2808 4350 4368
Size: 168 1606 142 122 358 412 1542 18
Fragments arranged by size:
1606 1542 412 358 168 142 122 18
TatI w'GTAC_w
Cuts at: 0 1298 4368
Size: 1298 3070
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 155 187 1905 1937 2481 2513 2621 2653
Size: 155 32 1718 32 544 32 108 32
Cuts at: 2653 3954 3978 3986 4010 4027 4059 4368
Size: 1301 24 8 24 17 32 309
Fragments arranged by size:
1718 1301 544 309 155 108 32 32
32 32 32 24 24 17 8
XhoI C'TCGA_G
Cuts at: 0 2027 4368
Size: 2027 2341
XmnI GAAnn'nnTTC
Cuts at: 0 995 1748 2163 3788 3798 4368
Size: 995 753 415 1625 10 570
Fragments arranged by size:
1625 995 753 570 415 10
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflII AflIII AhdI
AloI AlwNI ApaLI ApoI AseI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtsI DraI DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HincII HindIII KasI MmeI MslI MspA1I NarI NcoI
NdeI NheI NsiI NspI PflMI PfoI PpiI PpuMI
PshAI PsiI PstI PvuII SacI SanDI SbfI SfcI
SfoI SmlI SpeI SspI StuI StyI SwaI TaqII
TatI TstI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AgeI AleI AlfI ApaI AscI AsiSI
AvrII BbvCI BglI BsaBI BsgI BsiWI BsmBI BsrBI
BsrGI BssHII BtgZI ClaI CspCI DraIII EagI EciI
EcoRV FseI FspI FspAI HpaI KpnI MfeI MluI
MscI NaeI NgoMIV NotI NruI PacI PasI PciI
PmeI PmlI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SapI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SphI SrfI Tth111I XbaI XcmI XmaI ZraI