Restriction map for
pF1KE2516
(Linear) (Six-base) MAPSORT of: KIEE2516.seq Check: 6651 from: 1 to: 1113
KIEE2516.orf
With 182 enzymes: SgfI *
February 25, 2014 15:45 ..
AflIII A'CryG_T
Cuts at: 0 1018 1113
Size: 1018 95
AlwNI CAG_nnn'CTG
Cuts at: 0 414 692 1113
Size: 414 278 421
Fragments arranged by size:
421 414 278
AvaI C'yCGr_G
Cuts at: 0 252 419 426 486 1113
Size: 252 167 7 60 627
Fragments arranged by size:
627 252 167 60 7
BanI G'GyrC_C
Cuts at: 0 457 1113
Size: 457 656
BanII G_rGCy'C
Cuts at: 0 107 141 352 1113
Size: 107 34 211 761
Fragments arranged by size:
761 211 107 34
BbeI G_GCGC'C
Cuts at: 0 461 1113
Size: 461 652
BglI GCCn_nnn'nGGC
Cuts at: 0 324 515 1113
Size: 324 191 598
Fragments arranged by size:
598 324 191
Bme1580I G_kGCm'C
Cuts at: 0 776 827 1113
Size: 776 51 286
Fragments arranged by size:
776 286 51
BmgBI CAC'GTC
Cuts at: 0 1019 1113
Size: 1019 94
BmtI G_CTAG'C
Cuts at: 0 251 1113
Size: 251 862
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 835 1113
Size: 374 461 278
Fragments arranged by size:
461 374 278
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1090 1113
Size: 1090 23
BsaI GGTCTCn'nnnn_
Cuts at: 0 700 1113
Size: 700 413
BsaBI GATnn'nnATC
Cuts at: 0 677 1113
Size: 677 436
BsaHI Gr'CG_yC
Cuts at: 0 146 458 1113
Size: 146 312 655
Fragments arranged by size:
655 312 146
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 107 137 185 188 215 218 428 458
Size: 107 30 48 3 27 3 210 30
Cuts at: 458 1113
Size: 655
Fragments arranged by size:
655 210 107 48 30 30 27 3
3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 209 277 292 301 322 478 650
Size: 37 172 68 15 9 21 156 172
Cuts at: 650 847 1113
Size: 197 266
Fragments arranged by size:
266 197 172 172 156 68 37 21
15 9
BseYI C'CCAG_C
Cuts at: 0 28 34 1113
Size: 28 6 1079
Fragments arranged by size:
1079 28 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 1113
Size: 602 511
BsiEI CG_ry'CG
Cuts at: 0 57 525 1113
Size: 57 468 588
Fragments arranged by size:
588 468 57
BsiHKAI G_wGCw'C
Cuts at: 0 390 1113
Size: 390 723
BsmI GAATG_Cn'
Cuts at: 0 931 1113
Size: 931 182
Bsp1286I G_dGCh'C
Cuts at: 0 107 141 352 390 776 827 1113
Size: 107 34 211 38 386 51 286
Fragments arranged by size:
386 286 211 107 51 38 34
BsrBI CCG'CTC
Cuts at: 0 230 470 984 1113
Size: 230 240 514 129
Fragments arranged by size:
514 240 230 129
BsrDI GCAATG_nn'
Cuts at: 0 807 1113
Size: 807 306
BsrFI r'CCGG_y
Cuts at: 0 56 514 1113
Size: 56 458 599
Fragments arranged by size:
599 458 56
BsrGI T'GTAC_A
Cuts at: 0 1002 1113
Size: 1002 111
BssHII G'CGCG_C
Cuts at: 0 397 399 1113
Size: 397 2 714
Fragments arranged by size:
714 397 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 385 692 858 1113
Size: 385 307 166 255
Fragments arranged by size:
385 307 255 166
BstYI r'GATC_y
Cuts at: 0 1008 1113
Size: 1008 105
BtgI C'CryG_G
Cuts at: 0 87 200 623 1113
Size: 87 113 423 490
Fragments arranged by size:
490 423 113 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 798 1113
Size: 798 315
BtsI GCAGTG_nn'
Cuts at: 0 328 1113
Size: 328 785
DraIII CAC_nnn'GTG
Cuts at: 0 657 1113
Size: 657 456
EaeI y'GGCC_r
Cuts at: 0 54 1113
Size: 54 1059
EagI C'GGCC_G
Cuts at: 0 54 1113
Size: 54 1059
EarI CTCTTCn'nnn_
Cuts at: 0 290 299 435 1113
Size: 290 9 136 678
Fragments arranged by size:
678 290 136 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 78 1113
Size: 78 1035
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 835 1113
Size: 374 461 278
Fragments arranged by size:
461 374 278
EcoNI CCTnn'n_nnAGG
Cuts at: 0 875 1113
Size: 875 238
EcoO109I rG'GnC_Cy
Cuts at: 0 411 810 1113
Size: 411 399 303
Fragments arranged by size:
411 399 303
FspI TGC'GCA
Cuts at: 0 378 1113
Size: 378 735
HaeII r_GCGC'y
Cuts at: 0 338 461 1113
Size: 338 123 652
Fragments arranged by size:
652 338 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 188 220 855 887 1113
Size: 188 32 635 32 226
Fragments arranged by size:
635 226 188 32 32
KasI G'GCGC_C
Cuts at: 0 457 1113
Size: 457 656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 863 1113
Size: 192 671 250
Fragments arranged by size:
671 250 192
MslI CAynn'nnrTG
Cuts at: 0 847 1113
Size: 847 266
MspA1I CmG'CkG
Cuts at: 0 89 1113
Size: 89 1024
NaeI GCC'GGC
Cuts at: 0 58 516 1113
Size: 58 458 597
Fragments arranged by size:
597 458 58
NarI GG'CG_CC
Cuts at: 0 458 1113
Size: 458 655
NcoI C'CATG_G
Cuts at: 0 623 1113
Size: 623 490
NgoMIV G'CCGG_C
Cuts at: 0 56 514 1113
Size: 56 458 599
Fragments arranged by size:
599 458 56
NheI G'CTAG_C
Cuts at: 0 247 1113
Size: 247 866
PflMI CCAn_nnn'nTGG
Cuts at: 0 174 414 1113
Size: 174 240 699
Fragments arranged by size:
699 240 174
PfoI T'CCnGG_A
Cuts at: 0 725 1113
Size: 725 388
SacII CC_GC'GG
Cuts at: 0 90 1113
Size: 90 1023
SfcI C'TryA_G
Cuts at: 0 907 1113
Size: 907 206
SfoI GGC'GCC
Cuts at: 0 459 1113
Size: 459 654
SmaI CCC'GGG
Cuts at: 0 421 1113
Size: 421 692
SmlI C'TyrA_G
Cuts at: 0 1069 1113
Size: 1069 44
SrfI GCCC'GGGC
Cuts at: 0 421 1113
Size: 421 692
StyI C'CwwG_G
Cuts at: 0 623 876 1113
Size: 623 253 237
Fragments arranged by size:
623 253 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 549 636 1113
Size: 549 87 477
Fragments arranged by size:
549 477 87
TatI w'GTAC_w
Cuts at: 0 749 1002 1113
Size: 749 253 111
Fragments arranged by size:
749 253 111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 107 139 185 217 1113
Size: 107 32 46 32 896
Fragments arranged by size:
896 107 46 32 32
XmaI C'CCGG_G
Cuts at: 0 419 1113
Size: 419 694
XmnI GAAnn'nnTTC
Cuts at: 0 160 1113
Size: 160 953
Enzymes that do cut:
AflIII AlwNI AvaI BanI BanII BbeI BglI Bme1580I
BmgBI BmtI BpmI BpuEI BsaI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstYI BtgI BtgZI
BtsI DraIII EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I KasI MmeI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI PflMI PfoI SacII
SfcI SfoI SmaI SmlI SrfI StyI TaqII TatI
TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmrI BplI
Bpu10I BsaAI BsaWI BsiWI BsmBI BspEI BspHI BspMI
BssSI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraI DrdI EcoICRI EcoRI EcoRV FalI FseI FspAI
HincII HindIII HpaI KpnI MfeI MluI MscI NdeI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SspI StuI SwaI Tth111I XbaI XcmI XhoI
ZraI