(Linear) (Six-base) MAPSORT of: KIEE2508.seq  Check: 4700  from: 1  to: 1362



KIEE2508.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AccI GT'mk_AC

Cuts at:      0     271    1362 
   Size:        271    1091
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     366    1019    1362 
   Size:        366     653     343

  Fragments arranged by size: 

                 653     366     343
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1219    1362 
   Size:       1219     143
 
AseI AT'TA_AT

Cuts at:      0     328     400    1362 
   Size:        328      72     962

  Fragments arranged by size: 

                 962     328      72
 
AvaI C'yCGr_G

Cuts at:      0     204    1362 
   Size:        204    1158
 
BanII G_rGCy'C

Cuts at:      0    1241    1362 
   Size:       1241     121
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      32     836    1007    1060    1362 
   Size:         32     804     171      53     302

  Fragments arranged by size: 

                 804     302     171      53      32
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     483    1362 
   Size:        483     879
 
BfrBI ATG'CAT

Cuts at:      0     282     765    1362 
   Size:        282     483     597

  Fragments arranged by size: 

                 597     483     282
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     201    1333    1362 
   Size:        201    1132      29

  Fragments arranged by size: 

                1132     201      29
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     914    1362 
   Size:        914     448
 
BsaWI w'CCGG_w

Cuts at:      0     413    1362 
   Size:        413     949
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1297    1327    1362 
   Size:       1297      30      35

  Fragments arranged by size: 

                1297      35      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1321    1362 
   Size:       1321      41
 
Bsp1286I G_dGCh'C

Cuts at:      0    1241    1362 
   Size:       1241     121
 
BspHI T'CATG_A

Cuts at:      0    1044    1362 
   Size:       1044     318
 
BsrBI CCG'CTC

Cuts at:      0     696    1362 
   Size:        696     666
 
BsrDI GCAATG_nn'

Cuts at:      0     744     993    1362 
   Size:        744     249     369

  Fragments arranged by size: 

                 744     369     249
 
BsrGI T'GTAC_A

Cuts at:      0       2    1250    1362 
   Size:          2    1248     112

  Fragments arranged by size: 

                1248     112       2
 
BtsI GCAGTG_nn'

Cuts at:      0     106    1362 
   Size:        106    1256
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     366    1019    1362 
   Size:        366     653     343

  Fragments arranged by size: 

                 653     366     343
 
EcoO109I rG'GnC_Cy

Cuts at:      0     248    1362 
   Size:        248    1114
 
EcoRV GAT'ATC

Cuts at:      0     201    1362 
   Size:        201    1161
 
HincII GTy'rAC

Cuts at:      0     941    1362 
   Size:        941     421
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1047    1362 
   Size:       1047     315
 
MslI CAynn'nnrTG

Cuts at:      0     347     770    1046    1362 
   Size:        347     423     276     316

  Fragments arranged by size: 

                 423     347     316     276
 
MspA1I CmG'CkG

Cuts at:      0     850     906    1362 
   Size:        850      56     456

  Fragments arranged by size: 

                 850     456      56
 
NsiI A_TGCA'T

Cuts at:      0     284     767    1362 
   Size:        284     483     595

  Fragments arranged by size: 

                 595     483     284
 
PfoI T'CCnGG_A

Cuts at:      0    1133    1362 
   Size:       1133     229
 
PpuMI rG'GwC_Cy

Cuts at:      0     248    1362 
   Size:        248    1114
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1259    1291    1362 
   Size:       1259      32      71

  Fragments arranged by size: 

                1259      71      32
 
PvuII CAG'CTG

Cuts at:      0     850     906    1362 
   Size:        850      56     456

  Fragments arranged by size: 

                 850     456      56
 
SfcI C'TryA_G

Cuts at:      0     137     521    1169    1362 
   Size:        137     384     648     193

  Fragments arranged by size: 

                 648     384     193     137
 
SmlI C'TyrA_G

Cuts at:      0     204    1362 
   Size:        204    1158
 
SspI AAT'ATT

Cuts at:      0     338     513     668    1009    1362 
   Size:        338     175     155     341     353

  Fragments arranged by size: 

                 353     341     338     175     155
 
TatI w'GTAC_w

Cuts at:      0       2    1250    1362 
   Size:          2    1248     112

  Fragments arranged by size: 

                1248     112       2
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1297    1329    1362 
   Size:       1297      32      33

  Fragments arranged by size: 

                1297      33      32
 
XhoI C'TCGA_G

Cuts at:      0     204    1362 
   Size:        204    1158
 
XmnI GAAnn'nnTTC

Cuts at:      0     227    1362 
   Size:        227    1135
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     AseI     AvaI    BanII     BbsI    BciVI
    BfrBI     BmrI     BsaI    BsaWI    BsaXI     BsgI Bsp1286I    BspHI
    BsrBI    BsrDI    BsrGI     BtsI  Eco57MI EcoO109I    EcoRV   HincII
     MmeI     MslI   MspA1I     NsiI     PfoI    PpuMI     PsrI    PvuII
     SfcI     SmlI     SspI     TatI     TstI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BseRI    BseYI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI     FalI
     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII
  Tth111I     XbaI     XcmI     XmaI     ZraI