(Linear) (Six-base) MAPSORT of: KIEE2506.seq  Check: 3245  from: 1  to: 1368



KIEE2506.orf

 With 182 enzymes: SgfI * 

                        February 25, 2014 15:45  ..

AccI GT'mk_AC

Cuts at:      0     401     523    1368 
   Size:        401     122     845

  Fragments arranged by size: 

                 845     401     122
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     551     950     971    1368 
   Size:        551     399      21     397

  Fragments arranged by size: 

                 551     399     397      21
 
AflIII A'CryG_T

Cuts at:      0     396     642    1368 
   Size:        396     246     726

  Fragments arranged by size: 

                 726     396     246
 
AhdI GACnn_n'nnGTC

Cuts at:      0     354     490    1368 
   Size:        354     136     878

  Fragments arranged by size: 

                 878     354     136
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      20      52    1368 
   Size:         20      32    1316

  Fragments arranged by size: 

                1316      32      20
 
ApoI r'AATT_y

Cuts at:      0     333    1368 
   Size:        333    1035
 
AvaI C'yCGr_G

Cuts at:      0    1189    1368 
   Size:       1189     179
 
BamHI G'GATC_C

Cuts at:      0     621    1368 
   Size:        621     747
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     489    1058    1368 
   Size:        489     569     310

  Fragments arranged by size: 

                 569     489     310
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     738     772    1368 
   Size:        738      34     596

  Fragments arranged by size: 

                 738     596      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     568    1368 
   Size:        568     800
 
BclI T'GATC_A

Cuts at:      0    1086    1247    1368 
   Size:       1086     161     121

  Fragments arranged by size: 

                1086     161     121
 
BglII A'GATC_T

Cuts at:      0     288    1368 
   Size:        288    1080
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     245    1145    1368 
   Size:        245     900     223

  Fragments arranged by size: 

                 900     245     223
 
BsaAI yAC'GTr

Cuts at:      0     397    1368 
   Size:        397     971
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     497     527    1010    1040    1118    1148    1368 
   Size:        497      30     483      30      78      30     220

  Fragments arranged by size: 

                 497     483     220      78      30      30      30
 
BseYI C'CCAG_C

Cuts at:      0    1158    1368 
   Size:       1158     210
 
BspHI T'CATG_A

Cuts at:      0     420    1368 
   Size:        420     948
 
BsrBI CCG'CTC

Cuts at:      0     259    1368 
   Size:        259    1109
 
BsrDI GCAATG_nn'

Cuts at:      0     449     944    1368 
   Size:        449     495     424

  Fragments arranged by size: 

                 495     449     424
 
BstBI TT'CG_AA

Cuts at:      0     269     337    1368 
   Size:        269      68    1031

  Fragments arranged by size: 

                1031     269      68
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     709    1368 
   Size:        709     659
 
BstYI r'GATC_y

Cuts at:      0     107     288     621    1368 
   Size:        107     181     333     747

  Fragments arranged by size: 

                 747     333     181     107
 
BstZ17I GTA'TAC

Cuts at:      0     524    1368 
   Size:        524     844
 
DraI TTT'AAA

Cuts at:      0     888    1368 
   Size:        888     480
 
EaeI y'GGCC_r

Cuts at:      0     254    1176    1368 
   Size:        254     922     192

  Fragments arranged by size: 

                 922     254     192
 
EarI CTCTTCn'nnn_

Cuts at:      0      50     527    1348    1368 
   Size:         50     477     821      20

  Fragments arranged by size: 

                 821     477      50      20
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     245     551     950     971    1145    1368 
   Size:        245     306     399      21     174     223

  Fragments arranged by size: 

                 399     306     245     223     174      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0     900    1368 
   Size:        900     468
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     241    1368 
   Size:        241    1127
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     243     275     340     372     476     508    1118    1150 
   Size:        243      32      65      32     104      32     610      32

Cuts at:   1150    1368 
   Size:        218

  Fragments arranged by size: 

                 610     243     218     104      65      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0     800    1368 
   Size:        800     568
 
MscI TGG'CCA

Cuts at:      0    1178    1368 
   Size:       1178     190
 
NspI r_CATG'y

Cuts at:      0     570    1368 
   Size:        570     798
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1158    1368 
   Size:       1158     210
 
PfoI T'CCnGG_A

Cuts at:      0     999    1368 
   Size:        999     369
 
PspXI vC'TCGA_Gb

Cuts at:      0    1189    1368 
   Size:       1189     179
 
SapI GCTCTTCn'nnn_

Cuts at:      0     527    1368 
   Size:        527     841
 
SfcI C'TryA_G

Cuts at:      0     860    1368 
   Size:        860     508
 
SmlI C'TyrA_G

Cuts at:      0    1189    1368 
   Size:       1189     179
 
SspI AAT'ATT

Cuts at:      0     432     794    1368 
   Size:        432     362     574

  Fragments arranged by size: 

                 574     432     362
 
StyI C'CwwG_G

Cuts at:      0     501    1368 
   Size:        501     867
 
TatI w'GTAC_w

Cuts at:      0     694    1368 
   Size:        694     674
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     819     851    1368 
   Size:        819      32     517

  Fragments arranged by size: 

                 819     517      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     709    1368 
   Size:        709     659
 
XhoI C'TCGA_G

Cuts at:      0    1189    1368 
   Size:       1189     179
 
XmnI GAAnn'nnTTC

Cuts at:      0     221    1368 
   Size:        221    1147
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AhdI     AloI     ApoI     AvaI    BamHI
     BbsI     BcgI    BciVI     BclI    BglII     BpmI    BsaAI    BsaXI
    BseYI    BspHI    BsrBI    BsrDI    BstBI    BstXI    BstYI  BstZ17I
     DraI     EaeI     EarI  Eco57MI EcoO109I     FalI    Hin4I  HindIII
     MscI     NspI    PflMI     PfoI    PspXI     SapI     SfcI     SmlI
     SspI     StyI     TatI     TstI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BanI    BanII     BbeI    BbvCI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI   BstEII   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
    TaqII  Tth111I     XbaI     XmaI     ZraI