Restriction map for
pF1KE2501
(Linear) (Six-base) MAPSORT of: KIEE2501.seq Check: 7188 from: 1 to: 966
KIEE2501.orf
With 182 enzymes: SgfI *
February 16, 2012 13:35 ..
AccI GT'mk_AC
Cuts at: 0 104 966
Size: 104 862
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 792 966
Size: 792 174
AflIII A'CryG_T
Cuts at: 0 62 966
Size: 62 904
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 148 397 966
Size: 148 249 569
Fragments arranged by size:
569 249 148
AlwNI CAG_nnn'CTG
Cuts at: 0 408 519 727 966
Size: 408 111 208 239
Fragments arranged by size:
408 239 208 111
ApaI G_GGCC'C
Cuts at: 0 713 966
Size: 713 253
AvaI C'yCGr_G
Cuts at: 0 705 954 966
Size: 705 249 12
Fragments arranged by size:
705 249 12
BanII G_rGCy'C
Cuts at: 0 287 713 966
Size: 287 426 253
Fragments arranged by size:
426 287 253
BlpI GC'TnA_GC
Cuts at: 0 351 966
Size: 351 615
Bme1580I G_kGCm'C
Cuts at: 0 386 682 713 966
Size: 386 296 31 253
Fragments arranged by size:
386 296 253 31
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 672 966
Size: 672 294
Bpu10I CC'TnA_GC
Cuts at: 0 941 966
Size: 941 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 904 966
Size: 904 62
BsaAI yAC'GTr
Cuts at: 0 63 966
Size: 63 903
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 917 947 966
Size: 917 30 19
Fragments arranged by size:
917 30 19
BseYI C'CCAG_C
Cuts at: 0 401 966
Size: 401 565
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 966
Size: 793 173
BsiHKAI G_wGCw'C
Cuts at: 0 287 966
Size: 287 679
Bsp1286I G_dGCh'C
Cuts at: 0 287 386 682 713 966
Size: 287 99 296 31 253
Fragments arranged by size:
296 287 253 99 31
BspHI T'CATG_A
Cuts at: 0 845 966
Size: 845 121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 765 966
Size: 765 201
BsrDI GCAATG_nn'
Cuts at: 0 83 966
Size: 83 883
BstAPI GCAn_nnn'nTGC
Cuts at: 0 519 727 966
Size: 519 208 239
Fragments arranged by size:
519 239 208
BstXI CCAn_nnnn'nTGG
Cuts at: 0 750 966
Size: 750 216
BstYI r'GATC_y
Cuts at: 0 188 515 966
Size: 188 327 451
Fragments arranged by size:
451 327 188
Bsu36I CC'TnA_GG
Cuts at: 0 671 966
Size: 671 295
BtgI C'CryG_G
Cuts at: 0 162 749 966
Size: 162 587 217
Fragments arranged by size:
587 217 162
BtsI GCAGTG_nn'
Cuts at: 0 565 725 966
Size: 565 160 241
Fragments arranged by size:
565 241 160
EaeI y'GGCC_r
Cuts at: 0 258 489 860 966
Size: 258 231 371 106
Fragments arranged by size:
371 258 231 106
EarI CTCTTCn'nnn_
Cuts at: 0 145 966
Size: 145 821
EcoICRI GAG'CTC
Cuts at: 0 285 966
Size: 285 681
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 792 966
Size: 792 174
EcoNI CCTnn'n_nnAGG
Cuts at: 0 847 966
Size: 847 119
EcoO109I rG'GnC_Cy
Cuts at: 0 167 709 710 832 840 852 966
Size: 167 542 1 122 8 12 114
Fragments arranged by size:
542 167 122 114 12 8 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 382 414 966
Size: 382 32 552
Fragments arranged by size:
552 382 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 960 966
Size: 960 6
MscI TGG'CCA
Cuts at: 0 260 491 966
Size: 260 231 475
Fragments arranged by size:
475 260 231
MslI CAynn'nnrTG
Cuts at: 0 379 617 901 966
Size: 379 238 284 65
Fragments arranged by size:
379 284 238 65
MspA1I CmG'CkG
Cuts at: 0 816 966
Size: 816 150
NcoI C'CATG_G
Cuts at: 0 162 749 966
Size: 162 587 217
Fragments arranged by size:
587 217 162
PpuMI rG'GwC_Cy
Cuts at: 0 167 966
Size: 167 799
PspOMI G'GGCC_C
Cuts at: 0 709 966
Size: 709 257
PstI C_TGCA'G
Cuts at: 0 524 723 966
Size: 524 199 243
Fragments arranged by size:
524 243 199
PvuII CAG'CTG
Cuts at: 0 816 966
Size: 816 150
SacI G_AGCT'C
Cuts at: 0 287 966
Size: 287 679
SapI GCTCTTCn'nnn_
Cuts at: 0 145 966
Size: 145 821
SbfI CC_TGCA'GG
Cuts at: 0 524 966
Size: 524 442
SfcI C'TryA_G
Cuts at: 0 309 520 719 966
Size: 309 211 199 247
Fragments arranged by size:
309 247 211 199
SmlI C'TyrA_G
Cuts at: 0 919 954 966
Size: 919 35 12
Fragments arranged by size:
919 35 12
StyI C'CwwG_G
Cuts at: 0 162 497 749 835 966
Size: 162 335 252 86 131
Fragments arranged by size:
335 252 162 131 86
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 897 966
Size: 897 69
XhoI C'TCGA_G
Cuts at: 0 954 966
Size: 954 12
XmnI GAAnn'nnTTC
Cuts at: 0 55 966
Size: 55 911
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI ApaI AvaI BanII
BlpI Bme1580I BplI Bpu10I BpuEI BsaAI BsaXI BseYI
BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BstAPI BstXI
BstYI Bsu36I BtgI BtsI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I Hin4I MmeI MscI MslI MspA1I NcoI
PpuMI PspOMI PstI PvuII SacI SapI SbfI SfcI
SmlI StyI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BmgBI BmrI BmtI BpmI
BsaI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmI
BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI TstI Tth111I XbaI XmaI ZraI