Restriction map for
pF1KE2491
(Linear) (Six-base) MAPSORT of: KIEE2491.seq Check: 9497 from: 1 to: 7059
KIEE2491.orf
With 182 enzymes: SgfI *
September 4, 2015 15:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3648 7059
Size: 3648 3411
AatII G_ACGT'C
Cuts at: 0 2201 3103 7059
Size: 2201 902 3956
Fragments arranged by size:
3956 2201 902
AccI GT'mk_AC
Cuts at: 0 1553 4556 5513 7059
Size: 1553 3003 957 1546
Fragments arranged by size:
3003 1553 1546 957
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1402 2110 2328 2367 2545 2695 2769 2848
Size: 1402 708 218 39 178 150 74 79
Cuts at: 2848 3178 5017 5197 5206 5355 6872 7059
Size: 330 1839 180 9 149 1517 187
Fragments arranged by size:
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178 150 149 79 74 39 9
AfeI AGC'GCT
Cuts at: 0 394 6748 7059
Size: 394 6354 311
Fragments arranged by size:
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AflIII A'CryG_T
Cuts at: 0 506 1034 4643 5795 7059
Size: 506 528 3609 1152 1264
Fragments arranged by size:
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AgeI A'CCGG_T
Cuts at: 0 3842 7059
Size: 3842 3217
AhdI GACnn_n'nnGTC
Cuts at: 0 1707 3099 7059
Size: 1707 1392 3960
Fragments arranged by size:
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AleI CACnn'nnGTG
Cuts at: 0 1067 4972 5224 7059
Size: 1067 3905 252 1835
Fragments arranged by size:
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AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3028 7059
Size: 3028 4031
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 847 879 2857 2889 7059
Size: 847 32 1978 32 4170
Fragments arranged by size:
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AlwNI CAG_nnn'CTG
Cuts at: 0 1711 1789 2606 2910 3749 4479 6118 7059
Size: 1711 78 817 304 839 730 1639 941
Fragments arranged by size:
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ApaI G_GGCC'C
Cuts at: 0 1767 3400 3563 3819 4239 4384 5662 6517
Size: 1767 1633 163 256 420 145 1278 855
Cuts at: 6517 7059
Size: 542
Fragments arranged by size:
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145
ApaLI G'TGCA_C
Cuts at: 0 1540 1714 3234 4839 5669 5902 6055 7059
Size: 1540 174 1520 1605 830 233 153 1004
Fragments arranged by size:
1605 1540 1520 1004 830 233 174 153
ApoI r'AATT_y
Cuts at: 0 2185 7059
Size: 2185 4874
AscI GG'CGCG_CC
Cuts at: 0 47 1661 7059
Size: 47 1614 5398
Fragments arranged by size:
5398 1614 47
AvaI C'yCGr_G
Cuts at: 0 97 688 1044 1498 1621 1766 2304 2452
Size: 97 591 356 454 123 145 538 148
Cuts at: 2452 3556 4239 5662 5999 6817 7059
Size: 1104 683 1423 337 818 242
Fragments arranged by size:
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337 242 148 145 123 97
AvrII C'CTAG_G
Cuts at: 0 6091 7059
Size: 6091 968
BamHI G'GATC_C
Cuts at: 0 650 6105 7059
Size: 650 5455 954
Fragments arranged by size:
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BanI G'GyrC_C
Cuts at: 0 36 102 187 275 1069 1502 1630 1825
Size: 36 66 85 88 794 433 128 195
Cuts at: 1825 1924 2705 3191 3406 3794 4162 4736 5719
Size: 99 781 486 215 388 368 574 983
Cuts at: 5719 5811 5823 5875 6715 7059
Size: 92 12 52 840 344
Fragments arranged by size:
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368 344 215 195 128 99 92 88
85 66 52 36 12
BanII G_rGCy'C
Cuts at: 0 97 137 160 1219 1767 2141 3271 3400
Size: 97 40 23 1059 548 374 1130 129
Cuts at: 3400 3563 3779 3819 4239 4384 5662 5890 5981
Size: 163 216 40 420 145 1278 228 91
Cuts at: 5981 6160 6350 6517 6530 6565 6607 6701 6712
Size: 179 190 167 13 35 42 94 11
Cuts at: 6712 7059
Size: 347
Fragments arranged by size:
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216 190 179 167 163 145 129 97
94 91 42 40 40 35 23 13
11
BbeI G_GCGC'C
Cuts at: 0 106 1634 2709 3410 4166 4740 5723 5827
Size: 106 1528 1075 701 756 574 983 104
Cuts at: 5827 6719 7059
Size: 892 340
Fragments arranged by size:
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106 104
BbsI GAAGACnn'nnnn_
Cuts at: 0 228 711 2734 2836 3897 3969 4956 4974
Size: 228 483 2023 102 1061 72 987 18
Cuts at: 4974 5339 7059
Size: 365 1720
Fragments arranged by size:
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72 18
BbvCI CC'TCA_GC
Cuts at: 0 3536 3982 7017 7059
Size: 3536 446 3035 42
Fragments arranged by size:
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BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 6589 6623 7059
Size: 6589 34 436
Fragments arranged by size:
6589 436 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 666 7059
Size: 666 6393
BclI T'GATC_A
Cuts at: 0 1121 1229 3888 7059
Size: 1121 108 2659 3171
Fragments arranged by size:
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BglI GCCn_nnn'nGGC
Cuts at: 0 226 1520 1771 2549 3404 3612 7059
Size: 226 1294 251 778 855 208 3447
Fragments arranged by size:
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BlpI GC'TnA_GC
Cuts at: 0 2140 2422 6773 7059
Size: 2140 282 4351 286
Fragments arranged by size:
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Bme1580I G_kGCm'C
Cuts at: 0 41 1314 1505 1544 1718 1767 1828 1937
Size: 41 1273 191 39 174 49 61 109
Cuts at: 1937 2045 2074 2118 2289 3194 3238 3390 3400
Size: 108 29 44 171 905 44 152 10
Cuts at: 3400 3453 3563 3819 4239 4384 4843 5662 5673
Size: 53 110 256 420 145 459 819 11
Cuts at: 5673 5906 6007 6059 6517 6905 7059
Size: 233 101 52 458 388 154
Fragments arranged by size:
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233 191 174 171 154 152 145 110
109 108 101 61 53 52 49 44
44 41 39 29 11 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 1469 2014 3352 3395 3783 5535 6014 6821
Size: 1469 545 1338 43 388 1752 479 807
Cuts at: 6821 6981 7059
Size: 160 78
Fragments arranged by size:
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78 43
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 402 7059
Size: 402 6657
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 2140 2494 2533 3127 3217 3411 3432
Size: 427 1713 354 39 594 90 194 21
Cuts at: 3432 3453 3793 3958 5113 5341 5608 5665 5920
Size: 21 340 165 1155 228 267 57 255
Cuts at: 5920 6222 6715 7018 7059
Size: 302 493 303 41
Fragments arranged by size:
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41 39 21 21
Bpu10I CC'TnA_GC
Cuts at: 0 3536 3982 4279 5353 5485 6419 6711 7017
Size: 3536 446 297 1074 132 934 292 306
Cuts at: 7017 7059
Size: 42
Fragments arranged by size:
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42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3445 4231 4930 7059
Size: 3445 786 699 2129
Fragments arranged by size:
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BsaI GGTCTCn'nnnn_
Cuts at: 0 3341 5854 6827 7059
Size: 3341 2513 973 232
Fragments arranged by size:
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BsaAI yAC'GTr
Cuts at: 0 312 509 1389 3894 7059
Size: 312 197 880 2505 3165
Fragments arranged by size:
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BsaBI GATnn'nnATC
Cuts at: 0 454 1108 2404 7059
Size: 454 654 1296 4655
Fragments arranged by size:
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BsaHI Gr'CG_yC
Cuts at: 0 82 103 419 743 1166 1631 2177 2198
Size: 82 21 316 324 423 465 546 21
Cuts at: 2198 2706 3100 3407 4163 4655 4737 5630 5720
Size: 508 394 307 756 492 82 893 90
Cuts at: 5720 5824 6716 7059
Size: 104 892 343
Fragments arranged by size:
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394 343 324 316 307 104 90 82
82 21 21
BsaWI w'CCGG_w
Cuts at: 0 2558 2839 3842 6369 7059
Size: 2558 281 1003 2527 690
Fragments arranged by size:
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BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 112 142 544 574 847 877 2834 2857
Size: 112 30 402 30 273 30 1957 23
Cuts at: 2857 2864 2887 3814 3844 4591 4621 6220 6250
Size: 7 23 927 30 747 30 1599 30
Cuts at: 6250 6673 6703 7059
Size: 423 30 356
Fragments arranged by size:
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112 30 30 30 30 30 30 30
23 23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 211 859 864 868 2470 2952 3130 4717
Size: 211 648 5 4 1602 482 178 1587
Cuts at: 4717 4735 4761 5110 5605 6280 6310 7059
Size: 18 26 349 495 675 30 749
Fragments arranged by size:
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211 178 30 26 18 5 4
BseYI C'CCAG_C
Cuts at: 0 183 953 1136 1821 2056 2064 2216 2276
Size: 183 770 183 685 235 8 152 60
Cuts at: 2276 3292 3454 3673 4436 4768 4781 5295 5961
Size: 1016 162 219 763 332 13 514 666
Cuts at: 5961 6285 6477 6749 6790 7059
Size: 324 192 272 41 269
Fragments arranged by size:
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272 269 235 219 192 183 183 162
152 60 41 13 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 927 980 2091 2739 4366 4591 4976 5346
Size: 927 53 1111 648 1627 225 385 370
Cuts at: 5346 7059
Size: 1713
Fragments arranged by size:
1713 1627 1111 927 648 385 370 225
53
BsiEI CG_ry'CG
Cuts at: 0 167 265 829 921 3616 3620 3648 6247
Size: 167 98 564 92 2695 4 28 2599
Cuts at: 6247 7059
Size: 812
Fragments arranged by size:
2695 2599 812 564 167 98 92 28
4
BsiHKAI G_wGCw'C
Cuts at: 0 137 311 907 1544 1718 2141 3017 3238
Size: 137 174 596 637 174 423 876 221
Cuts at: 3238 3271 4070 4640 4762 4843 4913 5464 5576
Size: 33 799 570 122 81 70 551 112
Cuts at: 5576 5673 5906 6059 6779 7059
Size: 97 233 153 720 280
Fragments arranged by size:
876 799 720 637 596 570 551 423
280 233 221 174 174 153 137 122
112 97 81 70 33
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1264 3858 3993 4255 6240 7059
Size: 1264 2594 135 262 1985 819
Fragments arranged by size:
2594 1985 1264 819 262 135
Bsp1286I G_dGCh'C
Cuts at: 0 41 97 137 160 311 907 1219 1314
Size: 41 56 40 23 151 596 312 95
Cuts at: 1314 1505 1544 1718 1767 1828 1937 2045 2074
Size: 191 39 174 49 61 109 108 29
Cuts at: 2074 2118 2141 2289 3017 3194 3238 3271 3390
Size: 44 23 148 728 177 44 33 119
Cuts at: 3390 3400 3453 3563 3779 3819 4070 4239 4384
Size: 10 53 110 216 40 251 169 145
Cuts at: 4384 4640 4762 4843 4913 5464 5576 5662 5673
Size: 256 122 81 70 551 112 86 11
Cuts at: 5673 5890 5906 5981 6007 6059 6160 6350 6517
Size: 217 16 75 26 52 101 190 167
Cuts at: 6517 6530 6565 6607 6701 6712 6779 6905 7059
Size: 13 35 42 94 11 67 126 154
Fragments arranged by size:
728 596 551 312 256 251 217 216
191 190 177 174 169 167 154 151
148 145 126 122 119 112 110 109
108 101 95 94 86 81 75 70
67 61 56 53 52 49 44 44
42 41 40 40 39 35 33 29
26 23 23 16 13 11 11 10
BspEI T'CCGG_A
Cuts at: 0 2558 7059
Size: 2558 4501
BspHI T'CATG_A
Cuts at: 0 1226 2396 2993 5438 5576 7059
Size: 1226 1170 597 2445 138 1483
Fragments arranged by size:
2445 1483 1226 1170 597 138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3032 3648 4085 4103 5501 6401 7059
Size: 3032 616 437 18 1398 900 658
Fragments arranged by size:
3032 1398 900 658 616 437 18
BsrBI CCG'CTC
Cuts at: 0 123 1043 3972 4823 5972 6014 6387 6532
Size: 123 920 2929 851 1149 42 373 145
Cuts at: 6532 7059
Size: 527
Fragments arranged by size:
2929 1149 920 851 527 373 145 123
42
BsrDI GCAATG_nn'
Cuts at: 0 5162 7059
Size: 5162 1897
BsrFI r'CCGG_y
Cuts at: 0 1909 3419 3615 3842 4682 4787 5496 5872
Size: 1909 1510 196 227 840 105 709 376
Cuts at: 5872 6029 6141 6242 6246 6376 7059
Size: 157 112 101 4 130 683
Fragments arranged by size:
1909 1510 840 709 683 376 227 196
157 130 112 105 101 4
BsrGI T'GTAC_A
Cuts at: 0 2939 7059
Size: 2939 4120
BssHII G'CGCG_C
Cuts at: 0 47 1661 5205 6541 7059
Size: 47 1614 3544 1336 518
Fragments arranged by size:
3544 1614 1336 518 47
BssSI C'ACGA_G
Cuts at: 0 2713 3040 7059
Size: 2713 327 4019
Fragments arranged by size:
4019 2713 327
BstAPI GCAn_nnn'nTGC
Cuts at: 0 275 941 1007 1789 3768 4569 7059
Size: 275 666 66 782 1979 801 2490
Fragments arranged by size:
2490 1979 801 782 666 275 66
BstEII G'GTnAC_C
Cuts at: 0 2209 2337 6144 6943 7059
Size: 2209 128 3807 799 116
Fragments arranged by size:
3807 2209 799 128 116
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1821 2017 3042 3936 4017 4173 4905 4932
Size: 1821 196 1025 894 81 156 732 27
Cuts at: 4932 7059
Size: 2127
Fragments arranged by size:
2127 1821 1025 894 732 196 156 81
27
BstYI r'GATC_y
Cuts at: 0 650 1976 2906 3076 4169 5939 6105 7059
Size: 650 1326 930 170 1093 1770 166 954
Fragments arranged by size:
1770 1326 1093 954 930 650 170 166
Bsu36I CC'TnA_GG
Cuts at: 0 1804 1854 6518 7059
Size: 1804 50 4664 541
Fragments arranged by size:
4664 1804 541 50
BtgI C'CryG_G
Cuts at: 0 230 298 1596 1689 2172 2388 2732 3468
Size: 230 68 1298 93 483 216 344 736
Cuts at: 3468 3601 4145 4547 4904 5414 5807 6184 6351
Size: 133 544 402 357 510 393 377 167
Cuts at: 6351 6620 7059
Size: 269 439
Fragments arranged by size:
1298 736 544 510 483 439 402 393
377 357 344 269 230 216 167 133
93 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3080 3915 7059
Size: 3080 835 3144
Fragments arranged by size:
3144 3080 835
BtsI GCAGTG_nn'
Cuts at: 0 1787 3574 5334 5478 6228 6463 7059
Size: 1787 1787 1760 144 750 235 596
Fragments arranged by size:
1787 1787 1760 750 596 235 144
DraIII CAC_nnn'GTG
Cuts at: 0 576 2239 6861 7059
Size: 576 1663 4622 198
Fragments arranged by size:
4622 1663 576 198
DrdI GACnn_nn'nnGTC
Cuts at: 0 6298 6820 7059
Size: 6298 522 239
Fragments arranged by size:
6298 522 239
EaeI y'GGCC_r
Cuts at: 0 164 227 262 826 918 1340 1811 1988
Size: 164 63 35 564 92 422 471 177
Cuts at: 1988 2019 2169 2402 3032 3173 3362 3471 3613
Size: 31 150 233 630 141 189 109 142
Cuts at: 3613 3617 4007 4142 5057 5567 6244 6374 7059
Size: 4 390 135 915 510 677 130 685
Fragments arranged by size:
915 685 677 630 564 510 471 422
390 233 189 177 164 150 142 141
135 130 109 92 63 35 31 4
EagI C'GGCC_G
Cuts at: 0 164 262 826 918 3613 3617 6244 7059
Size: 164 98 564 92 2695 4 2627 815
Fragments arranged by size:
2695 2627 815 564 164 98 92 4
EarI CTCTTCn'nnn_
Cuts at: 0 1367 2086 2776 3022 3832 4357 4867 6891
Size: 1367 719 690 246 810 525 510 2024
Cuts at: 6891 6952 7059
Size: 61 107
Fragments arranged by size:
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107 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 133 2641 3073 3745 4036 4714 5900 6275
Size: 133 2508 432 672 291 678 1186 375
Cuts at: 6275 6309 6487 7059
Size: 34 178 572
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2508 1186 678 672 572 432 375 291
178 133 34
EcoICRI GAG'CTC
Cuts at: 0 135 2139 3269 7059
Size: 135 2004 1130 3790
Fragments arranged by size:
3790 2004 1130 135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 1402 2110 2140 2328 2367 2494 2533
Size: 427 975 708 30 188 39 127 39
Cuts at: 2533 2545 2695 2769 2848 3127 3178 3217 3411
Size: 12 150 74 79 279 51 39 194
Cuts at: 3411 3432 3453 3793 3958 5017 5113 5197 5206
Size: 21 21 340 165 1059 96 84 9
Cuts at: 5206 5341 5355 5608 5665 5920 6222 6715 6872
Size: 135 14 253 57 255 302 493 157
Cuts at: 6872 7018 7059
Size: 146 41
Fragments arranged by size:
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255 253 194 188 165 157 150 146
135 127 96 84 79 74 57 51
41 39 39 39 30 21 21 14
12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 798 1682 2914 3043 4471 7031 7059
Size: 798 884 1232 129 1428 2560 28
Fragments arranged by size:
2560 1428 1232 884 798 129 28
EcoO109I rG'GnC_Cy
Cuts at: 0 1495 1763 2013 2124 2220 3333 3396 3397
Size: 1495 268 250 111 96 1113 63 1
Cuts at: 3397 3560 3816 4236 4380 4381 4476 4941 5349
Size: 163 256 420 144 1 95 465 408
Cuts at: 5349 5658 5659 6390 6513 6514 6656 6726 6822
Size: 309 1 731 123 1 142 70 96
Cuts at: 6822 7010 7059
Size: 188 49
Fragments arranged by size:
1495 1113 731 465 420 408 309 268
256 250 188 163 144 142 123 111
96 96 95 70 63 49 1 1
1 1
EcoRI G'AATT_C
Cuts at: 0 2185 7059
Size: 2185 4874
EcoRV GAT'ATC
Cuts at: 0 4271 7059
Size: 4271 2788
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4473 6234 7059
Size: 4473 1761 825
Fragments arranged by size:
4473 1761 825
FseI GG_CCGG'CC
Cuts at: 0 3619 7059
Size: 3619 3440
FspI TGC'GCA
Cuts at: 0 934 2650 3691 6397 7059
Size: 934 1716 1041 2706 662
Fragments arranged by size:
2706 1716 1041 934 662
HaeII r_GCGC'y
Cuts at: 0 106 396 995 1515 1634 2709 3410 3426
Size: 106 290 599 520 119 1075 701 16
Cuts at: 3426 3468 4166 4740 4788 5114 5723 5827 6719
Size: 42 698 574 48 326 609 104 892
Cuts at: 6719 6750 7059
Size: 31 309
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326 309 290 119 106 104 48 42
31 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 112 144 1156 1188 1693 1725 2454 2486
Size: 112 32 1012 32 505 32 729 32
Cuts at: 2486 3085 3117 3718 3750 3873 3905 6589 6621
Size: 599 32 601 32 123 32 2684 32
Cuts at: 6621 7059
Size: 438
Fragments arranged by size:
2684 1012 729 601 599 505 438 123
112 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2415 4557 4887 7059
Size: 2415 2142 330 2172
Fragments arranged by size:
2415 2172 2142 330
KasI G'GCGC_C
Cuts at: 0 102 1630 2705 3406 4162 4736 5719 5823
Size: 102 1528 1075 701 756 574 983 104
Cuts at: 5823 6715 7059
Size: 892 344
Fragments arranged by size:
1528 1075 983 892 756 701 574 344
104 102
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 1079 1349 3104 7059
Size: 542 537 270 1755 3955
Fragments arranged by size:
3955 1755 542 537 270
MscI TGG'CCA
Cuts at: 0 1342 1813 2021 2404 3034 4144 5059 7059
Size: 1342 471 208 383 630 1110 915 2000
Fragments arranged by size:
2000 1342 1110 915 630 471 383 208
MslI CAynn'nnrTG
Cuts at: 0 460 535 1067 1210 2398 2431 2500 2590
Size: 460 75 532 143 1188 33 69 90
Cuts at: 2590 3040 3250 4015 4138 4648 4897 4903 4972
Size: 450 210 765 123 510 249 6 69
Cuts at: 4972 5152 5224 6577 7059
Size: 180 72 1353 482
Fragments arranged by size:
1353 1188 765 532 510 482 460 450
249 210 180 143 123 90 75 72
69 69 33 6
MspA1I CmG'CkG
Cuts at: 0 272 776 1281 1293 1308 1364 1442 1454
Size: 272 504 505 12 15 56 78 12
Cuts at: 1454 1691 1795 1868 2068 2144 2216 2280 2348
Size: 237 104 73 200 76 72 64 68
Cuts at: 2348 3241 3431 3470 3746 4088 4436 5049 5841
Size: 893 190 39 276 342 348 613 792
Cuts at: 5841 6485 6622 6810 7059
Size: 644 137 188 249
Fragments arranged by size:
893 792 644 613 505 504 348 342
276 272 249 237 200 190 188 137
104 78 76 73 72 68 64 56
39 15 12 12
NaeI GCC'GGC
Cuts at: 0 3421 3617 4684 4789 5498 5874 6031 6244
Size: 3421 196 1067 105 709 376 157 213
Cuts at: 6244 6248 6378 7059
Size: 4 130 681
Fragments arranged by size:
3421 1067 709 681 376 213 196 157
130 105 4
NarI GG'CG_CC
Cuts at: 0 103 1631 2706 3407 4163 4737 5720 5824
Size: 103 1528 1075 701 756 574 983 104
Cuts at: 5824 6716 7059
Size: 892 343
Fragments arranged by size:
1528 1075 983 892 756 701 574 343
104 103
NcoI C'CATG_G
Cuts at: 0 298 1596 2732 4145 4904 6351 7059
Size: 298 1298 1136 1413 759 1447 708
Fragments arranged by size:
1447 1413 1298 1136 759 708 298
NgoMIV G'CCGG_C
Cuts at: 0 3419 3615 4682 4787 5496 5872 6029 6242
Size: 3419 196 1067 105 709 376 157 213
Cuts at: 6242 6246 6376 7059
Size: 4 130 683
Fragments arranged by size:
3419 1067 709 683 376 213 196 157
130 105 4
NotI GC'GGCC_GC
Cuts at: 0 262 7059
Size: 262 6797
NspI r_CATG'y
Cuts at: 0 321 357 648 897 939 1780 1987 2505
Size: 321 36 291 249 42 841 207 518
Cuts at: 2505 2802 4647 5205 5799 7059
Size: 297 1845 558 594 1260
Fragments arranged by size:
1845 1260 841 594 558 518 321 297
291 249 207 42 36
PasI CC'CwG_GG
Cuts at: 0 42 348 948 3392 4047 6150 7033 7059
Size: 42 306 600 2444 655 2103 883 26
Fragments arranged by size:
2444 2103 883 655 600 306 42 26
PciI A'CATG_T
Cuts at: 0 4643 5795 7059
Size: 4643 1152 1264
Fragments arranged by size:
4643 1264 1152
PflMI CCAn_nnn'nTGG
Cuts at: 0 2715 7039 7059
Size: 2715 4324 20
Fragments arranged by size:
4324 2715 20
PfoI T'CCnGG_A
Cuts at: 0 404 782 3278 5764 6428 7059
Size: 404 378 2496 2486 664 631
Fragments arranged by size:
2496 2486 664 631 404 378
PmlI CAC'GTG
Cuts at: 0 312 509 3894 7059
Size: 312 197 3385 3165
Fragments arranged by size:
3385 3165 312 197
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 847 879 2857 2889 7059
Size: 847 32 1978 32 4170
Fragments arranged by size:
4170 1978 847 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1495 2124 2220 3333 6390 6656 6726 6822
Size: 1495 629 96 1113 3057 266 70 96
Cuts at: 6822 7010 7059
Size: 188 49
Fragments arranged by size:
3057 1495 1113 629 266 188 96 96
70 49
PshAI GACnn'nnGTC
Cuts at: 0 3103 7059
Size: 3103 3956
PspOMI G'GGCC_C
Cuts at: 0 1763 3396 3559 3815 4235 4380 5658 6513
Size: 1763 1633 163 256 420 145 1278 855
Cuts at: 6513 7059
Size: 546
Fragments arranged by size:
1763 1633 1278 855 546 420 256 163
145
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1012 1044 4513 4545 7059
Size: 1012 32 3469 32 2514
Fragments arranged by size:
3469 2514 1012 32 32
PstI C_TGCA'G
Cuts at: 0 4082 5846 6043 6114 6167 6735 7059
Size: 4082 1764 197 71 53 568 324
Fragments arranged by size:
4082 1764 568 324 197 71 53
PvuI CG_AT'CG
Cuts at: 0 3648 7059
Size: 3648 3411
PvuII CAG'CTG
Cuts at: 0 272 1293 1364 3241 3431 3746 4088 5049
Size: 272 1021 71 1877 190 315 342 961
Cuts at: 5049 7059
Size: 2010
Fragments arranged by size:
2010 1877 1021 961 342 315 272 190
71
SacI G_AGCT'C
Cuts at: 0 137 2141 3271 7059
Size: 137 2004 1130 3788
Fragments arranged by size:
3788 2004 1130 137
SacII CC_GC'GG
Cuts at: 0 1692 3471 6623 7059
Size: 1692 1779 3152 436
Fragments arranged by size:
3152 1779 1692 436
SalI G'TCGA_C
Cuts at: 0 4555 7059
Size: 4555 2504
SanDI GG'GwC_CC
Cuts at: 0 1495 2220 3333 6726 6822 7010 7059
Size: 1495 725 1113 3393 96 188 49
Fragments arranged by size:
3393 1495 1113 725 188 96 49
SapI GCTCTTCn'nnn_
Cuts at: 0 1367 2086 2776 3022 4357 7059
Size: 1367 719 690 246 1335 2702
Fragments arranged by size:
2702 1367 1335 719 690 246
ScaI AGT'ACT
Cuts at: 0 556 1027 2380 4951 7059
Size: 556 471 1353 2571 2108
Fragments arranged by size:
2571 2108 1353 556 471
SexAI A'CCwGG_T
Cuts at: 0 1547 5738 7059
Size: 1547 4191 1321
Fragments arranged by size:
4191 1547 1321
SfcI C'TryA_G
Cuts at: 0 4078 5842 6039 6110 6163 6405 6731 7059
Size: 4078 1764 197 71 53 242 326 328
Fragments arranged by size:
4078 1764 328 326 242 197 71 53
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 226 3612 7059
Size: 226 3386 3447
Fragments arranged by size:
3447 3386 226
SfoI GGC'GCC
Cuts at: 0 104 1632 2707 3408 4164 4738 5721 5825
Size: 104 1528 1075 701 756 574 983 104
Cuts at: 5825 6717 7059
Size: 892 342
Fragments arranged by size:
1528 1075 983 892 756 701 574 342
104 104
SmaI CCC'GGG
Cuts at: 0 99 1500 1768 2306 3558 4241 5664 7059
Size: 99 1401 268 538 1252 683 1423 1395
Fragments arranged by size:
1423 1401 1395 1252 683 538 268 99
SmlI C'TyrA_G
Cuts at: 0 3460 4246 4945 7059
Size: 3460 786 699 2114
Fragments arranged by size:
3460 2114 786 699
SphI G_CATG'C
Cuts at: 0 321 648 897 939 2802 5205 7059
Size: 321 327 249 42 1863 2403 1854
Fragments arranged by size:
2403 1863 1854 327 321 249 42
SrfI GCCC'GGGC
Cuts at: 0 3558 7059
Size: 3558 3501
StuI AGG'CCT
Cuts at: 0 412 961 3811 3945 5694 6277 6508 6760
Size: 412 549 2850 134 1749 583 231 252
Cuts at: 6760 7059
Size: 299
Fragments arranged by size:
2850 1749 583 549 412 299 252 231
134
StyI C'CwwG_G
Cuts at: 0 221 298 470 1596 1895 2732 4145 4406
Size: 221 77 172 1126 299 837 1413 261
Cuts at: 4406 4505 4904 5882 6068 6091 6351 6737 7059
Size: 99 399 978 186 23 260 386 322
Fragments arranged by size:
1413 1126 978 837 399 386 322 299
261 260 221 186 172 99 77 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 175 238 490 1128 3093 4165 4330
Size: 19 156 63 252 638 1965 1072 165
Cuts at: 4330 4428 4506 4540 6526 7059
Size: 98 78 34 1986 533
Fragments arranged by size:
1986 1965 1072 638 533 252 165 156
98 78 63 34 19
TatI w'GTAC_w
Cuts at: 0 554 1025 1151 2378 2939 4451 4598 4949
Size: 554 471 126 1227 561 1512 147 351
Cuts at: 4949 7059
Size: 2110
Fragments arranged by size:
2110 1512 1227 561 554 471 351 147
126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 364 396 560 592 2834 2866 5977 6009
Size: 364 32 164 32 2242 32 3111 32
Cuts at: 6009 6049 6081 6090 6122 6220 6252 6673 6705
Size: 40 32 9 32 98 32 421 32
Cuts at: 6705 7059
Size: 354
Fragments arranged by size:
3111 2242 421 364 354 164 98 40
32 32 32 32 32 32 32 32
9
Tth111I GACn'n_nGTC
Cuts at: 0 28 4135 6660 7059
Size: 28 4107 2525 399
Fragments arranged by size:
4107 2525 399 28
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 754 1594 1806 2739 3192 6903 7059
Size: 754 840 212 933 453 3711 156
Fragments arranged by size:
3711 933 840 754 453 212 156
XmaI C'CCGG_G
Cuts at: 0 97 1498 1766 2304 3556 4239 5662 7059
Size: 97 1401 268 538 1252 683 1423 1397
Fragments arranged by size:
1423 1401 1397 1252 683 538 268 97
XmnI GAAnn'nnTTC
Cuts at: 0 862 2866 3844 7059
Size: 862 2004 978 3215
Fragments arranged by size:
3215 2004 978 862
ZraI GAC'GTC
Cuts at: 0 2199 3101 7059
Size: 2199 902 3958
Fragments arranged by size:
3958 2199 902
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PasI PciI
PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PsrI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
ScaI SexAI SfcI SfiI SfoI SmaI SmlI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AseI AsiSI BaeI BfrBI BglII
BmgBI BmtI BsiWI BsmI BstBI BstZ17I ClaI CspCI
DraI FspAI HindIII HpaI KpnI MfeI MluI NdeI
NheI NruI NsiI PacI PmeI PsiI PspXI RsrII
SbfI SgfI SgrAI SnaBI SpeI SspI SwaI XbaI
XhoI